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اسد معصومی اصل آمنه آریایی نژاد مسعود دهداری

چکیده

بابونههای آلمانی (Matricaria chamomilla L.) و شیرازی (Matricaria recutita L.) از مهم‌ترین گیاهان دارویی خانواده کاسنی می‌باشند که در صنایع داروسازی، بهداشتی، آرایشی و غذایی استفاده می‌شوند. ریزازدیادی درون شیشه‌ای گیاهان، پتانسیل بالایی جهت تولید داروهای گیاهی، احیاء و حفظ گیاهان، ایجاد تنوع سوماکلونی، تکثیر به صورت صنعتی، تولید متابولیت‌های ثانویه با ارزش و افزایش مواد موثره دارد. در این مطالعه تاثیر هورمون‌های گیاهی NAA، BAP، 2ip، Zeatin و کینتین بر باززایی مستقیم گیاهچه از ریزنمونه‌های برگ، کوتیلدون و ساقه (فاقد گره و دارای گره) بابونه‌های آلمانی و شیرازی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار مورد مطالعه قرار گرفت. نتایج نشان دادند که در باززایی مستقیم هر دو نوع بابونه بهترین ریزنمونه‌، ساقه دارای گره و کوتیلدون به ترتیب با 75/78 و 75 درصد شاخساره‌زایی بودند. همچنین برترین رقم و ترکیب هورمونی، رقم بابونه شیرازی با ترکیب هورمونی 5/1 میلی‌گرم در لیتر 2-ip به همراه 1/0 میلی‌گرم در لیترNAA در محیط کشت MS بود. تولید ریشه هم تحت‌تاثیر هورمون اکسین (5/0 میلی‌گرم در لیتر IBA) و هم به‌صورت خودبخودی انجام شد. بر اساس نتایج این تحقیق، بابونه پاسخ مناسبی به باززایی مستقیم نشان داد.

جزئیات مقاله

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ارجاع به مقاله
معصومی اصل ا., آریایی نژاد آ., & دهداری م. (2015). بررسی باززایی مستقیم در بابونه‌های آلمانی (Matricaria chamomilla L.) و شیرازی (Matricaria recutita L.) در شرایط درون شیشه ای. علوم باغبانی, 29(4), 601-609. https://doi.org/10.22067/jhorts4.v29i4.32417
نوع مقاله
علمی - پژوهشی