اثر پوشش‌دهی با عصاره‌ پوست انار و نشاء گوجه‌فرنگی بر ویژگی‌های فیتوشیمیایی و حفظ کیفیت میوه توت‌فرنگی رقم ’پاروس‘ طی نگهداری در انبار سرد

نوع مقاله : مقالات پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 گروه شیمی آلی، دانشکده شیمی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

ضایعات محصولات باغبانی از جدی‌ترین نگرانی‌های امروزی است. با افزایش جمعیت، خسارات و ضایعات محصولات غذایی نیز به‌طبع آن افزایش می­یابد که به‌شدت بر سلامت محیط‌زیست و انسان اثرگذار است. از طرفی ضایعات باغبانی سرشار از مواد مغذی، پلی ساکاریدها، ترکیبات ضد میکروبی است که می­توان از آنها برای تهیه پوشش­های خوراکی استفاده کرد. پوشش خوراکی از میوه‌ها در برابر اتلاف مواد مغذی و معدنی محافظت می‌کند و ماندگاری آن­ها را افزایش می‌دهد. توت‌فرنگی به‌دلیل تنفس و فعالیت متابولیکی بالا، بافت نرم و حساسیت به آسیب­های مکانیکی و قارچی دارای عمر پس از برداشت کوتاهی است. جایگزینی تیمارهای سازگار با محیط‌زیست و ایمن برای جلوگیری از تلفات پس از برداشت این میوه جهت بهبود ماندگاری در انبار مورد توجه پژوهشگران قرار گرفته است. در این پژوهش عصاره­های گیاهی استخراج شده از ضایعات بخش باغبانی به‌عنوان پوشش خوراکی توت­فرنگی استفاده شده است. بدین جهت تأثیر عصاره‌های گیاهی پوست انار و نشاء گوجه­فرنگی هر کدام با غلظت یک درصد بر افزایش زمان ماندگاری میوه توت­فرنگی به مدت 15 روز در دمای 4 درجه سانتی­گراد مورد بررسی قرار گرفت. لازم به ذکر است، از میوه فاقد پوشش خوراکی به‌عنوان تیمار کنترل استفاده شد. برخی صفات کیفی و شاخص‌های رنگ میوه پس از 5، 10 و 15 روز انبارداری مورد ارزیابی قرار گرفتند. نتایج نشان داد کاهش وزن و افت سفتی بافت در میوه­های پوشش­دهی شده با عصاره­های گیاهی کمتر از میوه‌های شاهد بود. از طرفی تیمارهای مورد استفاده اثر مثبتی بر ویژگی­های کیفی میوه شامل pH، مواد جامد محلول و اسیدیته قابل تیتراسیون داشتند. محتوی آنتوسیانین کل، میزان اسید آسکوربیک و فلاونوئید کل نمونه­ها در طول مدت انبارداری در میوه­های پوشش­دار بهتر از میوه‌های شاهد حفظ گردید. از طرفی محتوای فنل کل و ظرفیت آنتی­اکسیدانی تحت تأثیر تیمار عصاره­های گیاهی قرار نگرفت. بیشترین تغییرات رنگ در سطح میوه در نمونه شاهد مشاهده شد. میوه‌های پوشش­دهی شده با عصاره پوست انار در حفظ پارامترهای کیفی پس از 15 روز، نسبت به سایر تیمارها بهتر عمل کردند و درصد پوسیدگی میوه‌ها را کمتر کردند. یافته‌های این پژوهش نشان داد که غلظت­ یک درصد عصاره­های پوست انار و نشاء­ گوجه­فرنگی می‌تواند به عنوان پوشش خوراکی در حفظ ویژگی­های کیفی میوه توت­فرنگی در انبار سرد موثر باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Coating with Pomegranate Peel and Tomato Seedlings Extracts on the Phytochemical Traits and Maintaining the Quality of Strawberry Fruits cv. Paros During Cold Storage

نویسندگان [English]

  • S. Shirani Rad 1
  • M, Sayyari 1
  • M.A. Zolfigol 2
1 Horticultural Science Department, College of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2 Department of Organic Chemistry, Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran
چکیده [English]

Introduction
Horticultural waste is one of the top challenges these days. As the population increases, food loss and waste, which has a serious impact on the environment and human health. Horticultural waste is rich in nutrients, polysaccharides and antimicrobial compounds that can be used in the production of edible coatings. Edible coatings protect fruit from nutrient and mineral loss and extend shelf life. Strawberry fruit is one of the commercial horticultural crops because it contains important and diverse sources of natural antioxidants, flavonoids, phenolic acids and minerals. However, the fruit is highly perishable due to its high respiratory rate and metabolic activity, soft texture and lack of protective skin, which can lead to moisture loss, mechanical damage and fungal damage during harvesting, handling and packing. It is estimated that approximately 30% of strawberry fruit is wasted during the post-harvest stage before reaching the consumer. Therefore, reducing the destruction rate of its quantitative and qualitative properties is considered one of the most important challenges. Plant Extract Edible Coating (PEEC) is an environmentally friendly edible coating. Like other edible coatings, PEEC is a thin layer of material applied to the surface of a product. Pomegranate peel extract has biological activities such as antibacterial, antiviral, antioxidant, anti-inflammatory, and antifungal. This extract was used alone or in combination with other post-harvest treatments to preserve product quality. Tomatoes contain secondary metabolites called steroidal glycoalkaloids. These compounds primarily act as crop protection agents against insects, bacteria, parasites, viruses and fungi. This study evaluated the efficacy of pomegranate peel extract and tomato seedlings in maintaining strawberry fruit quality during cold storage and reducing post-harvest waste. 
Materials and Methods
Healthy fruits with uniform size, shape, and color were carefully selected from strawberries harvested from an orchard in Kamyaran, Kurdistan. We conducted a study to investigate the effects of coating these strawberries with pomegranate peel extract (1%) and tomato seedling extract (1%) on their physiological and qualitative responses. The fruits were coated with the respective plant extracts and subsequently stored at 4 ± 1°C and 90–95% relative humidity for a duration of 15 days. Strawberry quality was analyzed on the first day of storage and on days 5, 10 and 15. Various qualitative factors such as weight loss, firmness pH, total soluble solids content, titratable acidity, total phenolic content, total anthocyanin content, total antioxidant activity, total flavonoid content, ascorbic acid, color and decay severity were evaluated. Statistical analysis of the data was performed using SAS (version 9.4) and mean comparisons were performed using the Duncan multiple range test. 
Results and Discussion
The study on the property retention and long-term cold storage time of pomegranate peel and tomato seedling extracts coating showed that a concentration of 1% of the extracts used have a significant effect on strawberry fruit quality and phytochemical parameters. It was shown to have a significant impact on strawberry fruit quality and phytochemical parameters, improving compared to the control treatment during cultivation. Weight loss increased with all treatments during storage. After 5 days of storage, no differences between treatments were discernible, but at the end of storage all treatments showed a clear decrease in fruit weight. Pomegranate peel and tomato seedling extracts reduced weight loss by 12% and 15%, respectively, while the control  significantly reduced weight loss by 26%. Despite the decrease in fruit tissue firmness during storage, the firmness of the plant extract-coated fruit was maintained and significantly different from the control. PH remained at low levels for all treatments compared to controls. The total acid and total soluble solids content of the fruit are affected by the treatments considered, the storage, and the combination of times and treatments. The total content of phenolic compounds reached 223 mg gallic acid and 236 mg gallic acid per 100 g fresh weight on the 10th and 15th storage days after treatment with pomegranate peel extract. For tomato seedling extract, this corresponds to 207 mg and 182 mg gallic acid per 100 g fresh weight. The total anthocyanin content in all fruits decreases with increasing storage time, but this trend increases after 10 days when tomato seedlings are treated. In all fruits, various treatments increase anthocyanin levels throughout the storage time. ANOVA of antioxidant activity showed no significant effects on treatment-independent and chronotherapy-interaction effects, while the time-independent effect showed a significant effect at 5%. The greatest antioxidant activity is associated with pomegranate peel extract. The frequency of this feature in treatment decreased with increasing storage time. During the treatment period, there was a progressive increase in antioxidant activity from the 10th to the 15th day, demonstrating a significant difference compared to the beginning of the treatment. Average comparison results revealed a slight but significant difference in the treatments concerning flavonoid content. Analysis of variance and comparison of mean results indicated a significant difference in ascorbic acid content during storage among the different treatments. Color indices remained consistent across all treatments. The 15-day shelf life of strawberries was assessed, and the treatments employed effectively reduced decay rates during storage. Upon analysis, it was determined that the 1% concentration of pomegranate peel extract exhibited the highest efficacy in suppressing the severity of spoilage.
Conclusion
Residues from various agricultural sectors have a variety of uses, including their properties as preservatives that extend the shelf life of perishable fruits and enhance the nutritional value of fruits and vegetables. Replacing plant extracts with synthetic compounds can play an important role in preserving the characteristics and quality of strawberry fruits during storage. Based on the results of this study, an edible coating containing plant extracts from pomegranate peel and tomato seedling as natural preservatives was used to extend the shelf life and enhance the nutritional quality of strawberry fruits during cold storage. Finally, using natural compounds such as plant extracts from agricultural waste is a safe and healthy way to manage and preserve the properties of post-harvest agricultural products. 

کلیدواژه‌ها [English]

  • Fruit decay
  • Total anthocyanin content
  • Total antioxidant activity
  • Total phenolic compounds

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

  1. Akhond, M., Heidarizadeh, F., & Kolahi, M. (2022). Comparative study of qualitative and chemical characteristics of Camarosa and Parus strawberry cultivars during 15 days of storage. Developmental Biology, 14(2), 43-52. https://doi.org/10.30495/jdb.2022.1941563.1270
  2. Akhond, M., Kolahi, M., & Heidarizadeh, F. (2022). Investigation of phytochemical and antioxidant changes of two strawberry fruit cultivars during storage in 4 0C. Journal of food science and technology(Iran), 19(127), 255-266. https://doi.org/10.22034/fsct.19.127.255
  3. Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., & Lightfoot, D.A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6(4), 42.
  4. Asgari Marjanlu, A., Mostofi, Y., Shoeibi, S., & Maghoumi, M. (2009). Effect of basil (Ocimum basilicum) essential oil on gray mold control and postharvest quality of strawberry (cv. Selva). Journal of Medicinal Plants, 8(29), 131-139.
  5. Asghari, M., Azarsharif, Z., Tajik, H., & Farrokhzad, A. (2019) .Effect of galbanum gum coating combined with cumin essential oil and calcium chloride on quality and shelf life of sweet cherry (cv. Siah Mashhad). Horticultural Science, 32(4), 665-680.
  6. Ayhan, Z., & Eştürk, O. (2009). Overall quality and shelf life of minimally processed and modified atmosphere packaged “ready‐to‐eat” pomegranate arils. Journal of Food Science, 74(5), C399-C405. https://doi.org/10.1111/j.1750-3841.2009.01184.x
  7. Daraei Garmakhany, A., Mirzaei, H., & Shakarami, K. (2021). Investigation of the effect of flower and leaf ethanolic extract of Humulus lupulus plant on the shelf life and quality attributes of strawberry fruits. Journal of Food Science and Technology(Iran), 17(109), 75-90. https://doi.org/10.52547/fsct.17.109.75
  8. De Bruno, A., Gattuso, A., Ritorto, D., Piscopo, A., & Poiana, M. (2023). Effect of edible coating enriched with naturpal antioxidant extract and Bergamot essential oil on the shelf life of strawberries. Foods, 12(3), 488. https://doi.org/10.3390/foods12030488
  9. Ebrahimipour Bafghi, M., Dehestani-Ardakani, M., & Gholamnezhad, J. (2020). Effect of pomegranate peel extract, rosmarinus and artemisia essential oils on vase lLife of cut chrysanthemum (Chrysanthemum morifolium). Plant Productions, 43(1), 129-143.
  10. El-Miniawy, S., Ragab, M., Youssef, S., & Metwally, A. (2014). Influence of foliar spraying of seaweed extract on growth, yield and quality of strawberry plants. Journal of Applied Sciences Research, 10, 88-94.
  11. Fawole, O.A., Makunga, N.P., & Opara, U.L. (2012). Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract. BMC Complementary and Alternative Medicine, 12(1), 1-11. https://doi.org/10.1186/1472-6882-12-200
  12. Faz, F.N., Mirdehghan, S.H., Karimi, H., & Alaei, H. (2016). Eeffect of thymol and menthol essential oils combined with packaging with celofan on the maintenance of postharvest quality of strawberry cv. Parus. Iranian Journal of Horticultural Science, 47(1), 81-91. https://doi.org/10.22059/ijhs.2016.58214
  13. Feliziani, E., & Romanazzi, G. (2016). Postharvest decay of strawberry fruit: Etiology, epidemiology, and disease management. Journal of Berry Research, 6(1), 47-63. https://doi.org/10.3233/JBR-150113
  14. Figueiredo-González, M., Valentao, P., Pereira, D.M., & Andrade, P.B. (2017). Further insights on tomato plant: Cytotoxic and antioxidant activity of leaf extracts in human gastric cells. Food and Chemical Toxicology, 109, 386-392. https://doi.org/10.1016/j.fct.2017.09.018
  15. Fufa,D., Abera, S., Haile, A., & Kumar, V. (2019). Effect of using plant extracts with coating materials on physicochemical quality of tomato fruit (Solanum lycopersicum L.) stored at ambient temperature. Forte Journal of Agriculture, 1(1), 1-16.
  16. Ghorbani,, Shirzad, H., & Alirezalu, A. (2021). Effect of anisone essential oil on biochemical and shelf life properties of strawberry cultivar 'Albion' Journal of Plant Research, 34(2), 412-423.
  17. Ismail, T., Sestili, P., & Akhtar, S. (2012). Pomegranate peel and fruit extracts: a review of potential anti-inflammatory and anti-infective effects. Journal of Ethnopharmacology, 143(2), 397-405. https://doi.org/10.1016/j.jep.2012.07.004
  18. Kaderides, K., Kyriakoudi, A., Mourtzinos, I., & Goula, A.M. (2021). Potential of pomegranate peel extract as a natural additive in foods. Trends in Food Science & Technology, 115, 380-390. https://doi.org/10.1016/j.tifs.2021.06.050
  19. Kharchoufi, S., Parafati, L., Licciardello, F., Muratore, G., Hamdi, M., Cirvilleri, G., & Restuccia, C. (2018). Edible coatings incorporating pomegranate peel extract and biocontrol yeast to reduce Penicillium digitatum postharvest decay of oranges. Food Microbiology, 74, 107-112. https://doi.org/10.1016/j.fm.2018.03.011
  20. Li, J.-W., Ding, S.-D., & Ding, X.-L. (2005). Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube. Process Biochemistry, 40(11), 3607-3613. https://doi.org/10.1016/j.procbio.2005.03.005
  21. Malviya, S., Jha, A., & Hettiarachchy, N. (2014). Antioxidant and antibacterial potential of pomegranate peel extracts. Journal of Food Science and Technology, 51, 4132-4137. https://doi.org/10.1007/s13197-013-0956-4
  22. Mohammadrezakhani, S., Pakkish, Z., & Rafeii, S. (2016). Role of brassinosteroid on qualitative characteristics improvement of strawberry fruit cv. Paros. Journal of Horticultural Science30(2), 316-326.
  23. Moshari-Nasirkandi, A., Alirezalu, A., & Hachesu, M.A. (2020). Effect of lemon verbena bio-extract on phytochemical and antioxidant capacity of strawberry (Fragaria× ananassa cv. Sabrina) fruit during cold storage. Biocatalysis and Agricultural Biotechnology, 25, 101613. https://doi.org/10.1016/j.bcab.2020.101613
  24. Mozafari, A.A., Rahimi, R., & Abdousi, V. (2017). Effects of Echinophora platyloba essential oil on quantitative and qualitative characteristics of two varieties of strawberries during shelf- life. Jourrnal of Food Reaserch, 24, 87-102.
  25. Nair, M.S., Saxena, A., & Kaur, C. (2018). Characterization and antifungal activity of pomegranate peel extract and its uUse in polysaccharide-based edible coatings to extend the shelf-Life of capsicum (Capsicum annuum). Food and Bioprocess Technology, 11(7), 1317-1327. https://doi.org/10.1007/s11947-018-2101-x
  26. Nair, M.S., Saxena, A., & Kaur, C. (2018). Effect of chitosan and alginate based coatings enriched with pomegranate peel extract to extend the postharvest quality of guava (Psidium guajava). Food Chemistry, 240, 245-252. https://doi.org/10.1016/j.foodchem.2017.07.122
  27. Mubarb Mayvan, S., Abedi, B., & Moghadam, M. (2017). The effect of green tea and pomegranate peel extracts on increasing the storage time of Thomson Novel oranges inoculated with Penicillium fungal isolates. Paper presented at the The fifth international conference of new ideas in agriculture, environment and tourism.
  28. Pardini, A., Consumi, M., Leone, G., Bonechi, C., Tamasi, G., Sangiorgio, P., & Magnani, A. (2021). Effect of different post-harvest storage conditions and heat treatment on tomatine content in commercial varieties of green tomatoes. Journal of Food Composition and Analysis, 96, https://doi.org/10.1016/j.jfca.2020.103735
  29. Pashazadeh, B., Rad, A.H.E., Najjary, H.H., & Shariaei, P. (2021). Examination of chitosan and extracted coating on qualitative, physicochemical and microbial properties of apple fruit during cold storage. Journal of Food Processing and Preservation, 13(4), 23-42. https://doi.org/10.22069/EJFPP.2022.18777.1651
  30. Petriccione, M., Mastrobuoni, F., Pasquariello, M.S., Zampella, L., Nobis, E., Capriolo, G., & Scortichini, M. (2015). Effect of chitosan coating on the postharvest quality and antioxidant enzyme system response of strawberry fruit during cold storage. Foods, 4(4), 501-523. https://doi.org/10.3390/foods4040501
  31. Poraziz, S., Nazoori, F., Mirdehghan, S.H., & Esmailizadeh, M. (2019). ffect of sodium alginate on the shelf life of strawberry fruits (Fragaria ananassacv. Gaviota). Iranian Journal of Horticultural Science, 50(3), 515-526.
  32. Quintana, S.E., Llalla, O., García-Risco, M.R., & Fornari, T. (2021). Comparison between essential oils and supercritical extracts into chitosan-based edible coatings on strawberry quality during cold The Journal of Supercritical Fluids, 171, 105198. https://doi.org/10.1016/j.supflu.2021.105198
  33. Regnier, T., Combrinck, S., & Du Plooy, W. (2012). Essential oils and other plant extracts as food preservatives. Progress in Food Preservation, 539-579. https://doi.org/10.1002/9781119962045.ch26
  34. Rezaei, M., Abdollahi, F., Dastjerdi, A., & Yousefzadi, M. (2019). Effect of Ulva flexuosa Wulfen Seaweed and Shirazi Thyme (Zataria multiflora) extracts on qualitative characteristics of Washington Navel Orange under sorage period. Research and Innovation in Food Science and Technology, 8, 245-258(3).
  35. Saleem, M.S., Anjum, M.A., Naz, S., Ali, S., Hussain, S., Azam, M., & Ejaz, S. (2021). Incorporation of ascorbic acid in chitosan-based edible coating improves postharvest quality and storability of strawberry fruits. International Journal of Biological Macromolecules, 189, 160-169. https://doi.org/10.1016/j.ijbiomac.2021.08.051
  36. Saleh, I., & Abu-Dieyeh, M. (2022). Novel Prosopis juliflora leaf ethanolic extract coating for extending postharvest shelf-life of strawberries. Food Control, 133, 108641. https://doi.org/10.1016/j.foodcont.2021.108641
  37. Sarebani, A., Arshad, M., & Nazari Deljo, M.J. (2020). The Effect of postharvest methyl jasmonate treatment on ethylene biosynthesis, antioxidant capacity and shelf Life of strawberry. Journal of Crop Production and Processing, 10(2), 93-107. https://doi.org/10.47176/jcpp.10.2.33972
  38. Sarkhosh, A., Zamani, Z., Fatahi, R., Ghorbani, H., & Hadian, J. (2007). A review on medicinal characteristics of pomegranate (Punica granatum). Journal of Medicinal Plants, 6(22), 13-24.
  39. Saxena, A., Sharma, L., & Maity, T. (2020). Enrichment of edible coatings and films with plant extracts or essential oils for the preservation of fruits and vegetables. In Biopolymer-based Formulations, 859-880. https://doi.org/10.1016/B978-0-12-816897-4.00034-5
  40. Shahbaz, M. U., Arshad, M., Mukhtar, K., Nabi, B. G., Goksen, G., Starowicz, M., Manzoor, M. F. (2022). Natural plant extracts: an update about novel spraying as an alternative of chemical pesticides to extend the postharvest shelf life of fruits and vegetables. Molecules, 27(16), 5152. https://doi.org/10.3390/molecules27165152
  41. Sayyari, M., & Gharibi, R. (2016). Effects of Lavender Essential Oil and Methyl Salicylate on Gray Mold Control and Postharvest Quality of StrawbJournal Of Horticultural Science29(4), 662-670.
  42. Shahi, T., Ghorbani, M., Jafari, S. M., Sadeghi Mahoonak, A., Maghsoudlou, Y., & Beigbabaei, A. (2022). Effect of chitosan nano-coating loaded with pomegranate peel extract on physicochemical and microbial characteristics of pomegranate arils during Journal of food science and technology(Iran), 19(126), 71-85. https://doi.org/10.22034/fsct.19.126.71
  43. Shahimoridi, A., & Dastjerdi, A.M.M. (2020). The effects of ethanol extract of red mangrove and eucalyptus leaves on antioxidant capacity, enzyme activity and malondialdehyde of fresh banana fruit. Journal of Food Researches, 30(1), 15-28.
  44. Shirzadi, H., Aboutalebi, A.H., & Mohammadi, A.H. (2013). Effect of medicinal exxences oil plant on postharvest life and quality of Valencia sweet orange (Citrus chinensisValencia) in ambient storage. Postharvest biology and Technology of Horticultural Crop, 1(3), 12-11.
  45. Sogvar, O.B., Saba, M.K., & Emamifar, A. (2016). Aloe vera and ascorbic acid coatings maintain postharvest quality and reduce microbial load of strawberry fruit. Postharvest Biology and Technology, 114, 29-35. https://doi.org/10.1016/j.postharvbio.2015.11.019
  46. Sowmyashree, A., Sharma, R., Rudra, S.G., & Grover, M. (2021). Layer-by-Layer coating of hydrocolloids and mixed plant extract reduces fruit decay and improves postharvest life of nectarine fruits during cold storage. Acta Physiologiae Plantarum, 43(8), 112. https://doi.org/10.1007/s11738-021-03256-8
  47. Taherpour, L., Hosseinifarahi, M., & Radi, M. (2020). Application of pomegranate peel extract (PPE) with sodium alginate (Alg-Na) coating on fruit decay control and quality postharvest of sweet lemon fruit cv Mahali. Journal of Food Technology and Nutrition, 17, 107-122.
  48. Yang, C., Lu, J.-H., Xu, M.-T., Shi, X.-C., Song, Z.-W., Chen, T.-M., & Shahriar, M. (2022). Evaluation of chitosan coatings enriched with turmeric and green tea extracts on postharvest preservation of strawberries. Lwt, 163, 113551. https://doi.org/10.1016/j.lwt.2022.113551
  49. Zahran, A., Hassanein, R.A., & AbdelWahab, A.T. (2015). Effect of chitosan on biochemical composition and antioxidant activity of minimally processed ‘Wonderful’pomegranate arils during cold storage. Journal of Applied Botany and Food Quality, 88, 241-248.

 

CAPTCHA Image