moslem salehi; Vahid reza Saffari; shima hasanzadeh fard
Abstract
Introduction: Bedding plants, especially petunia is important element for urban landscaping and attracted the attention of landscapers. This is due to some properties such as growth habit and color. The petunia (Petunia hybrida L.) belongs to Solanaceae family that has annual and perennial varieties. ...
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Introduction: Bedding plants, especially petunia is important element for urban landscaping and attracted the attention of landscapers. This is due to some properties such as growth habit and color. The petunia (Petunia hybrida L.) belongs to Solanaceae family that has annual and perennial varieties. This plant is originally from Argentina, Brazil and Uruguay. Some plant growth regulators such as gibberellic acid (GA3) and vitamins including thiamine and ascorbic acid affect plant growth and development and may extend flowering period. Vitamin C affects cell division and cell growth in plants and is effective on the feeding cycle activity in higher plants and it has an important role in electron transport system. The concentrations of 50 and 100 ppm of vitamin C and thiamine can increase the plant height, leaf number, leaf area, fresh and dry weight, and chemical compounds of the Syngonium plant. The application of 100 mg/l of GA3 significantly increased plant height and the number of leaves of gladiolus.
Material and methods: The experiment was arranged in a factorial based on a completely randomized design with five replications. In this research, growth characteristics (lateral branch number, flower number, flower diameter, stem diameter, root length, and lateral branch length) and biochemical characteristics (chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, and reducing sugar) were measured. After seeding and transplanting the seedling at 6 leaf stage, plants sprayed at 4 various growth stages with following treatments:
1- Control
2- Vitamin C(100 mg/l)
3- Thiamin (100 mg/l)
4- Gibberellic acid (100 mg/l)
5- Vitamin C and Thiamin (The concentration of both 100 mg/l)
6- Vitamin C and Gibberellic acid (The concentration of both 100 mg/l)
7- Thiamin and Gibberellic acid (The concentration of both 100 mg/l)
8- Vitamin C, Thiamin and Gibberellic acid (The concentration of three 100 mg/l)
Data obtained from the measured parameters analyzed using statistical package SAS and means were compared using LSD test at the level of statistical significance of P˂0.05.
Results and Discussion: The results indicated that GA3 affected all the measured characteristics except carotenoids in comparison with thiamine and ascorbic acid. Meanwhile, the combined application of GA3 and ascorbic acid affected stem growth, root growth and flower diameter. Simultaneous use of GA3 and thiamine was effective on pigments, reducing sugar, carotenoids and flower number. The simultaneous application of three chemicals resulted in a considerable increase in growth and development traits, such as stem and root growth, flower number and diameter, pigments and reducing sugar compared to control. Generally, gibberellic acid will increase vegetative growth with impress cellular processes, including stimulation cell division and cell elongation. It was reported that the uses of ascorbic acid and gibberellic acid in Thyme (Thymus vulgaris) increased plant height and root length. Ascorbic acid makes a set of functions such as cell division and enlargements, cell wall development and other developmental processes in plants. Ascorbic acid affects plasma membrane proton pump and according to the acidic theory stimulates weaking cell wall and consequently will increase cell wall development and cell enlargement. It was found that thiamine significantly increased flower number in chrysanthemum .
Conclusion: The results indicated that the synergistic effects of this material can be used to enhance the growth and development of petunia. On the other hand, in arid and semiarid areas of the country, that drought and salinity stresses somewhat inevitable, these materials, especially vitamins are effective in reducing stress and increase flowering period.
F. Baniasadi; Vahid reza Saffari
Abstract
Introduction: Catharanthus roseus (L.) belongs to Apocynaceae family is an important medicinal plant and also cultivated as an ornamental plant almost throughout the tropical and subtropical areas all over the world. Recently, the uses of natural substances are considered very helpful to improve plant ...
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Introduction: Catharanthus roseus (L.) belongs to Apocynaceae family is an important medicinal plant and also cultivated as an ornamental plant almost throughout the tropical and subtropical areas all over the world. Recently, the uses of natural substances are considered very helpful to improve plant growth and development. Application of plant growth regulators (PGRs) and vitamins are reported in many horticultural crops. Ascorbic acid is the most abundant antioxidant in plant which protects plant cells. This substance affects cell differentiation and growth. Now a day it is considered as a plant growth regulator. Thiamine or vitamin B1 is water soluble and one of the B complex vitamins. This study was conducted to investigate the effect of the ascorbic acid, thiamine, BA and GA on growth and biochemical parameters of periwinkle.
Materials and Methods: The experiment was perform as a completely randomized design with 5 replications at research greenhouse of Shahid Bahonar University of Kerman in 2014.The treatments used include spraying water (control), ascorbic acid 100 mg.l-1, gibberellic acid 100 mg.l-1, thiamine 100 mg.l-1, BA 200 mg. l-1 and combinations of two, three and four of them to determine the response of plants to these substances alone or in combination of them. When the seedlings become on 6-leavs stage, the first foliar spray was carried out. In other two steps was repeated with interval of 10 days. In this study, longevity of the flower, flower diameter, the number of lateral branches, branches length, fresh and dry weight, chlorophyll content and reduced sugars were measured. Chlorophyll content and reduced sugars were measured according to method of Somogy (1952) and Lichtenthder (1987), respectively.
Results and Discussion: The results showed that the effect of thiamine on the number of flowers was more pronounced than other substances. The lowest number of flowers per plant was found in plants treated with gibberellic acid. All growth promoter substances increased flower diameter. Combined application of BAwith gibberellic acid had more influence on this parameter than other treatments, and increase flower diameter about 25%. Ascorbic acid plays an important role in cell division, plasma membrane, proton pumps and enlargement cell. According to the theory of acidic growth, ascorbic acid weakens cell wall and increases cell enlargement. Among the applied treatments combination of banzyladenine, thiamine and ascorbic acid increased the number of lateral branches to 100%. Results showed that GA in all concentrations and combinations used in this experiment increased lateral branches length. Several reports in plants such as croton, hibiscus and pot marigold indicated that BA can increase the number of branches per plant. Therefore, BA is used to enhance branching and to reduce the total height of the plants. The highest fresh and dry weight were observed in plants treated with the combinations of BA, thiamine and ascorbic acid. Thiamine increased chlorophyll a content more than other substances. There was no significant difference between thiamine or thiamine combined with ascorbic acid treatment on chlorophyll b content. Thiamine in combinations with all the other treatments significantly improved carotenoids content compared to control. Chlorophyll plays a key role in plants for the absorption and utilization of light energy which used in photosynthesis. Therefore, effects of PGRs on chlorophyll biosynthesis or degradation influence directly on photosynthesis. Thiamine was reported that in some plants assist resynthesize of chlorophyll in plant without chlorophyll and increased growth and yield eventually. All the treatments significantly increased reducing sugar. Although all of the substances improved the amount of reducing sugar, the role of thiamine was more dramatic compared to other treatments. The greatest amount of reducing sugar 85.3 mg. g-1 fresh weight was observed in the combination of BA, thiamine and ascorbic acid. Thiamine is an essential part of the biosynthesis of pyrophosphate thiamine coenzyme which has an important role in carbohydrate metabolism. Application of vitamin c it was reported the increased soluble sugar in Chinese hibiscus.
Conclusion: Periwinkle is a bedding plant that the number of flowers and lateral branches is very important. BA with thiamine and ascorbic acid combination has the best effects on these traits and improved growth condition of it. On the other hand, application of gibberellic acid, either simple or combined with other materials, due to the excessive increase in plant height, led to the bending of the plant.
S. Mansor Bahmani; Vahid reza Saffari; A.A. Maghsoudi Moud
Abstract
Production of onion all thruough the year particularly in semi tropical regions has been considered as an alternative to fulfil the local markets needs. In order to determine the best amount and method of urea fertilizer application so that to have higher yeild and lower amount of nitrate content the ...
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Production of onion all thruough the year particularly in semi tropical regions has been considered as an alternative to fulfil the local markets needs. In order to determine the best amount and method of urea fertilizer application so that to have higher yeild and lower amount of nitrate content the effects of seven levels of urea fertilizer including 0, 90, 180, 270, 360, 450 and 540 kg of nitrogen per ha and three different partitioning method of fertilizer application including application at 2, 3 and 4 times of each of the above mentioned amounts of fertilizers during the growing period were examined in a factorial experiment based on randomized complete block design with three replications in Jiroft. Results showed that increasing the amount of nitrogen fertilizer increased leaf length and crown diameter but decreased dry matter. The amount of fertilizer also showed significant effect on diameter and length. The highest bulb dry meter and length and yield were obtianed by application of 270 kg/ha of nitrogen while using higher amounts caused them to decrease. Nitrate content of the bulbs was strongly affected by the amount of nitrogen fertilizer applied so that the highest value (126.8 mg/kg/ fresh weight) was obtianed under highest level of nitrogen fertilizer. Nitrate content and bulb dry matter were also affected by the number of times of fertilizer application. Results of this study suggesting that to obtain high yield and low nitrate content, more than 180 kg of nitrogen per ha should not be applied.
