Marjan Hosseini; Seyyed Morteza Zahedi; Mahdieh Karimi; Asghar Ebrahimzadeh
Abstract
Introduction: Mango (Mangifera indica) is a tropical fruit native to India whose global production in 2014 reached nearly 45 million tones. Mango is a commercially important fruit and improvement in its storage is of special importance. Mango is a Climacteric fruit whose ripening is done by exogenous ...
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Introduction: Mango (Mangifera indica) is a tropical fruit native to India whose global production in 2014 reached nearly 45 million tones. Mango is a commercially important fruit and improvement in its storage is of special importance. Mango is a Climacteric fruit whose ripening is done by exogenous or endogenous ethylene. In plants, Polyamines such as spermine, spermidine, and putrescine contradict ethylene because of a common precursor (s-adenosyl methionine (SAM). During ripening, different qualitative and nutritional changes occur in the fruit, e. g. changes in color, tissue softening, accumulation of sugars and organic acids, and great changes in taste, flavor, aroma and plant biochemical materials. Fruit ripening is a complicated process, complementary to fruit development, and a start to its senescence. In general, senescence of a fruit is related to loss of membrane lipids, destabilization of membrane matrix, and lipid peroxidation. Recently, naturally active biological products are applied in a large amount for increasing the storage life and quality of the fruits and delaying their senescence.This study was carried out to investigate the effect of different concentrations of spermidine on the quality and vase life of a local mango variety of Minab.
Materials and Methods: Healthy fruits, uniform in size, shape, color, and degree of maturity were selected from a mango orchard in Minab and their original physical and chemical characteristics on the first day were measured after washing with water and drying. Statistical analysis of data was done by a general linear model (GLM) with SAS (version 9.1) and mean comparisons were performed using Duncan's multiple range test. Treatment solution in the rate of 0, 0.5, 1, and 2 mM spermidine (SIGMA) was made and its pH was set to 5 using NaOH. One liter of distilled water was used in the control treatment. The treated samples were immersed in solutions of different concentrations of spermidine just once for 30 minutes. During 24-days of shelf life, storage temperature was 15 °C and the relative humidity was 85 to 90 percent. Measurements were on the zero, 8th, 16th, and 24th days. Characteristics such as weight, firmness, flavor index, phenol, ascorbic acid, qualitative characteristics (PH, TA and TSS), and sensory evaluation were measured.
Results and Discussion: The results showed that physical and biochemical qualities in the control fruit were lower compared to the other treatments. The Polyamine treatment with spermidine, especially at the concentration of 2 mM, significantly maintained weight loss and reduction of vitamin C during storage of fruits. Spermidine treatments increased ascorbic acid and other organic acids in fruit juice and reduced pH. Increased acidity in spermidine treatments of these substances play an active role in coping with storage stress. Moreover, with reduction of tissue respiration, the consumption of organic acids decreased during storage. The results showed that increasing the concentration of spermidine leads to the least decrease in the phenol flesh. Firmness gradually decreased during storage and at the end of shelf life, there was significant difference between the treatments. It was found that treatments with 1 and 2 mM spermidine were the best. Furthermore, the effect of spermidine on the total soluble solid solutions in the flesh of mango fruit was not significant during storage. But skin color, taste, flavor, and aroma index were more favorable. Treatments that delay production of structural lipids led to the production of aromatic volatile substances, which produced a favorable aroma in fruits. It seems that increasing spermidine concentration plays an important role in mango fruit fragrance at the end of shelf-life. The 2 mM spermidine treatment led to the highest total content of phenol and showed the lowest pH in fruit juice.
Conclusions: Spermidine, that is naturally present in animals and plants in particular, belongs to the amine groups. The results indicated that spermidine maintains firmness and extends shelf life of mango fruits and has a significant and beneficial impact on the quality characteristics including weight, color, taste, aroma, and vitamin C during storage. It seems that spermidine of 2 mM concentration has tangible impact on mango fruits and it is recommended in mango store rooms. There is a competition in production of Ethylene and polyamides of spermine, spermidine, and putrescine in plants, due to their common precursor namely S-adenosyl methionine, yet they act oppositely in ripening and senescence processes. Application of polyamides had extraordinary effects on the quality of some fruits during storage.
Mohammad Ali Askari; Mesbah Babalar; Marjan Sadat Hosseini; Majid Amani Beni
Abstract
The current study was conducted in order to evaluate the effect of Mono and Di-potassium phosphate combination (MDKP) on some quantitative and qualitative characteristics of Iranian pomegranate (CV. Malas-e-Saveh) in Pomegranate Research Center of Saveh city, in 2012. To adjust the pH of the solution, ...
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The current study was conducted in order to evaluate the effect of Mono and Di-potassium phosphate combination (MDKP) on some quantitative and qualitative characteristics of Iranian pomegranate (CV. Malas-e-Saveh) in Pomegranate Research Center of Saveh city, in 2012. To adjust the pH of the solution, the Mono Potassium Phosphate and Di-Potassium Phosphate combined with each other in the ratio of 1:3, and their combined effects (Mono and Di-potassium phosphate) were studied. This experiment was carried out based on randomized complete block design (RCBD) with four concentrations of MDKP (0, 0.05, 0.1 and 0.2%) at two stages (Full bloom stage and one month after full bloom) and three replications per treatment. The results showed that foliar spray at concentrations of 0.1% and 0.2% resulted in significant increase in yield per tree, fruit number, Titrable Acidity (TA) compared to control treatment, so that 0.1% spraying caused 12/16% increase in yield per tree and 9/17% enhancement in fruit number in comparison to control treatment. Furthermore, foliar spray brought about significant enhancement in aril juice and color intensity of extract in which the highest amount of aril juice and color intensity of extract was obtained in 0.2 % and 0.1 % treatments, respectively. Foliar spray at 0.2 % concentration resulted in the reduction of Total Soluble Solids/Titrable Acidity ratio by 10% compared to control treatment. In conclusion, the results of this study show that MDKP spray at 0.1 % concentration was more effective than other concentrations.
Mesbah Babalar; Marjan Sadat Hosseini; Mohammad Ali Askari; Sohrab Davarpanah
Abstract
Pears are among the most important fruits in all the temperate regions in about 50 countries of the world. "Shahmiveh" and "Spadona" pears, sprayed at three stages at harvest with different concentrations putrescine (0, 0.5, 1 and 2 mM) and post harvest dipping at the same concentrations putrescine and ...
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Pears are among the most important fruits in all the temperate regions in about 50 countries of the world. "Shahmiveh" and "Spadona" pears, sprayed at three stages at harvest with different concentrations putrescine (0, 0.5, 1 and 2 mM) and post harvest dipping at the same concentrations putrescine and also dip into heat treatment (40 and 50 °C and control). Fruits were stored at 0±1 °C for 5 month. The factorial experiment was conducted using a completely randomized design of three replications. During the storage period, Sampling were carried out every three weeks and some of the qualitative and quantitative traits such as surface color, weight loss, firmness, pH, titrable acidity, total soluble solids, flavor index and vitamin C were measured. Results showed in Shahmiveh ratio to Spadona cultivar was upper firmness, pH and flavor index. Hue angle, lightness, weight loss, TA and TSS in Spadona cultivar was higher than Shahmiveh cultivar. 1 and 2 mM putrescine treatments showed maximum firmness and minimum pH, TSS and flavor index in both cultivars. Heat treatment at 40 and 50 °C respectively in Shahmiveh and Spadona increased TSS and fruit flavor index. In total, Spadona with suitable quality and quantity properties for the purpose of marketing and keeping in store is better than Shahmiveh.
