Ayda Taghizad; Jafar Ahmadi; Rahim Haddad; Mohammad Mahdi Zarrabi
Abstract
Abstract
Pistachio is one of the most important horticultural crops of Iran. The best way to have high yields is genetically pure and monogenous gardens. Thereby, study of genetic diversity makes this possible to have homogenous gardens with high performance genotypes. In the present study, the genetic ...
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Abstract
Pistachio is one of the most important horticultural crops of Iran. The best way to have high yields is genetically pure and monogenous gardens. Thereby, study of genetic diversity makes this possible to have homogenous gardens with high performance genotypes. In the present study, the genetic diversity among nineteen cultivars was assessed using inter-simple sequence repeats (ISSR) primers. The survey was done by 20 ISSR primers, out of them 10 with polymorphic bands was selected for genotyping. With 10 ISSR primers, 114 DNA fragments (loci) were produced that 73 of them (64.03%) were polymorphic. The mean of polymorphism information content (PIC) for the primers was ranged from 85% to 91%. The genetic similarity matrix was constructed using Jaccard coefficient. The genetic distance was ranged from 0.53 (low similarity) between kale- ghochi, hosein-khani and ebrahim-abadi up to 0.83 (high similarity) between kale-ghochi and ahmad-aghai. Based on cophenetic correlation coefficients, the best Clustering dendrogram was constructed by unweighted pair group method using arithmetic average (UPGMA). Cluster analysis revealed three main groups with 2, 4 and 13 pistachio varieties in each group. The present study showed that the use of ISSR markers for investigation on genetic diversity and providing genetic identifications of different Iranian pistachio cultivars could be useful and informatics.
Keywords: ISSR, Genetic diversity, Marker, Pistachio
Mohammad Mahdi Zarrabi; Ali Reza Talaie; Ali Soleimani; Rahim Haddad
Abstract
Abstract
Drought is one of the important environmental stresses affecting plants growth. In order to determine olives molecular tolerance to drought in greenhouse conditions, a factorial experiment was carried out based on randomized complete block design with two factors including stress with two levels: ...
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Abstract
Drought is one of the important environmental stresses affecting plants growth. In order to determine olives molecular tolerance to drought in greenhouse conditions, a factorial experiment was carried out based on randomized complete block design with two factors including stress with two levels: control and treatment, - 1.5 MPa and olive cultivars with six levels (Nabali, Gordal, Arbequine, Zard, Roghani and Feshomi) with three replications. In this study, some indices, such as protein, content peroxidase enzymes, ascorbate peroxidase, betaine content, chlorophyll and the number of stomata were measured. Results showed that drought stress reduced the total soluble protein content and it was different in various cultivars. Also drought stress caused a significant accumulation of peroxidase enzyme in olive leaves. Biochemical results showed that Gordal and Zard cultivars were more tolerant in comparison to other cultivars. The Betaine content showed a significant difference in the drought stress treated cultivars compared to the control ones. Chlorophyll a and b and the total chlorophyll content decreased under stress and this reduction was more in Gordal and Nabali than the other cultivars. Stomata density decreased under drought stress. An increase in stomata density appeared in Gordal and Roghani cultivars. It could be concluded that Nabali and Gordal cultivars are relatively more tolerant to drought stress.
Keywords: Olive, Stress, Peroxidase enzyme, Betaine, chlorophyll
