Shadi Attar; Gholamhossein Davarynejad; Leila Samiee; Mohammad Moghadam
Abstract
Introduction: Persian walnut (Juglans regia L.), belonging to the Juglandaceae family, has its natural origin in the mountainous regions of central Asia and especially northern forests of Iran. Most walnut genotypes are seedling and sexually reproduced. Conducting studies on the genetic structure of ...
Read More
Introduction: Persian walnut (Juglans regia L.), belonging to the Juglandaceae family, has its natural origin in the mountainous regions of central Asia and especially northern forests of Iran. Most walnut genotypes are seedling and sexually reproduced. Conducting studies on the genetic structure of these genotypes to identify, select and maintain their genetic resources is important. Identifying and collecting local varieties of fruit trees is considered as the first step on the path of breeding programs and lack of information regarding plants genetic characteristics causes the breeding work to be done slowly. Various methods have been used for studying genetic diversity and determining the genetic relationship between European and Asian varieties of walnut and identifying commercial walnut varieties, among which we can mention: Morphologic indices, Alozyme, Isozym, RFLP, RAPD, AFLP and ISSR markers. ISSR molecular marker was used in order to investigate genetic diversity of some genotypes of Persian walnut (Juglans regia L.) in Mashhad orchards. .
Materials and methods: To begin with, about 56 walnut trees from 4 orchards in Mashhad (Esteghlal (1), Golestan (2), Alandasht (3) and Emam Reza (4)) were selected and tagged from 2014 to 2016. In the spring of 2014 with the beginning of trees growth and opening of leaves, a number of leaves from each genotype were collected. After DNA extraction, the quality of samples by agarose gel (1 percentage) and electrophoresis method and quantity of them via spectrophotometer device at 260 and 280 nm wavelengths were determined. First, 24 primers of ISSR marker were prepared and after initial evaluation on 5 random genotypes, 9 primers with high polymorphism and repeatability were selected for further investigation. For PCR reaction, Amplicon kit (code 180 301, made in Denmark) was used. Gel electrophoresis images of primers that produced polymorphic bands with suitable resolution were analyzed manually. After scoring the bands, in which 0 used for showing the absence of a band and 1 showing the presence of a band in each sample, 1 and 0 numbers were changed to matrix by using NTedit software. Genetic similarities of samples were calculated by using NTSYSpc software, SIMQUAL method and DICE similarity coefficient. Dendrogram by cluster analysis was drawn by using UPGMA method. Principle coordinate analysis (PCO) was performed using the NTSYS software and grouping samples were evaluated in a two-dimensional plot.
Results and discussion: Results showed that from 9 primers in total, 118 bands amplified were in 300 to 3000 base pairs, while 29 bands were polymorphic. The number of amplified fragment for each primer was different so that UBC 844 (14 bands) had the highest and UBC 890 (8 bands) had the lowest amplified bands. The average amplified fragments for each primer was 9.83. The percentage of polymorphic in various primers was different. Maximum polymorphism (80%) of the primers was observed in UBC830. In general, according to the percentage of polymorphic bands, low adjustment to any changes in the environment was indicated. This can be used as an indicator to determine the value of diversity and genetic erosion. In genotypes cluster analysis, clustering was performed based on Dice similarity coefficient and UPGMA method, and 10 clusters were formed. ISSR molecular marker somewhat revealed genetic diversity among walnuts genotypes, whereas the genetic diversity was lower than expected. In general, by reviewing the findings in other parts of the world about walnut genetic diversity and comparing them with the results of this study, despite existing high genetic diversity among walnuts in many areas, some reports of low genetic diversity among walnuts populations have been published and unfortunately in recent years these reports has made more attention. Based on the results of several studies reporting low genetic diversity among walnuts, the following factors can be effective in this problem: natural disasters, human impacts such as deforestation and selection and propagation of superior genotypes, and sometimes walnuts self - pollination. In this respect, there is concern that if this trend of decreasing genetic diversity in the walnut population continues, this invaluable crop will be in danger of extinction. So we should think about a remedy. Finally, this investigation can be used as a start for conducting more researches in the region to maintain and manage this valuable crop germplasm and maximize genetic diversity for performing breeding programs in the future.
Shadi Attar; Gholamhossein Davarynejad; Seyyed Hossein Nemati
Abstract
Cross pollination in some of apples cultivar exists; also Iran is near the origin of apple trees so, large genetic diversity can be seen between Iranian cultivar. Also very famous apple cultivar is known in Iran for special flavor and taste. The name of this cultivar is Golab. This research was studied ...
Read More
Cross pollination in some of apples cultivar exists; also Iran is near the origin of apple trees so, large genetic diversity can be seen between Iranian cultivar. Also very famous apple cultivar is known in Iran for special flavor and taste. The name of this cultivar is Golab. This research was studied in the years 2010 and 2011 in Khorasan Razavi Province on Golab apple ecotypes to evaluate characteristics of Golab apples in Khorasan region, providing keywords for Identification, collect valuable ecotypes, preventing extinction and also use in apple breeding. Ecotypes were including: Golab Torbat Heidariyeh, Golab Sabz Pishras, Golab Kermanshah, Golab Kohanz, Golab Bahareh Shoghan. 31 qualitative and quantitative traits about fruit characteristics in a completely randomized design with 10 replicates to valuate these ecotypes were investigated. Average comparison showed that the greatest variability between cultivars was at the traits like: fruit weight, fruit firmness, fruit skin thickness, pH and TSS. The correlation results showed that the most significant positive correlation exists between fruit weight and stylar end width (0.92±0.06), fruit tail and fruit weight (0.87±0.07), Length to width ratio of fruit with fruit weight (0.85±0.08).
Shadi Attar; Gholamhossein Davarynejad; Seyyed Hossein Nemati
Abstract
Recognition and identification of many native and foreign apples cultivars are not clear for researchers, students, and gardeners. Additionally, since there is not any descriptor available on this case, they have different local names and sometimes similar in Khorasan-e-Razavi province. This study aimed ...
Read More
Recognition and identification of many native and foreign apples cultivars are not clear for researchers, students, and gardeners. Additionally, since there is not any descriptor available on this case, they have different local names and sometimes similar in Khorasan-e-Razavi province. This study aimed to evaluate physico-chemical and morphological traits to offer descriptor for identification some native and foreign apple varieties. Native varieties including Bijandi Akhlamad, Abdolmajidi, Golshahi Pish Ras, Compooti, Alimoori and foreign cultivars including Prime Rose, Red Spur, Fuji, Braebern, and Ingrid grafted on MM.106 rootstock during two years (2010-2011) at Golestan garden in Chenaran, Mashhad. About 62 different qualitative and quantitative traits related to the tree properties, leaves, flowers and physico-chemical attributes of fruit were carried out in a completely randomized design with 10 replications. Results showed that the fruit shape of Prime Rose cultivar was flat, Red Spur was conical to globular, Bijandi Akhlamadi was globular and the other was flat globular. The shape of carpel in Alimoori and Prime Rose were completely open and in the others were closed. Data showed that the greatest variation between cultivars was at the traits such as blossom end depth, fruit firmness, skin thickness, fruit length, fruit diameter, seed length, leaf width, fruit acidity and pH. Correlation analysis showed that petiole length significantly correlated with leaf length to leaf width ratio (r=0.74±0.07) and fruit weight with fruit length (r=0.96±0.03) positively at 1% probability level.
Zeinab Arian Pouya; Gholamhossein Davarynejad; Shadi Attar
Abstract
Abstract
Chilling injury of vegetative and generative buds, bark and wood during fall and winter seasons are mainly limiting factors for productivity of fruits. Selecting and expanding cultivars for more yield and quality without attention to the rate of their frost resistance are impossible. In this ...
Read More
Abstract
Chilling injury of vegetative and generative buds, bark and wood during fall and winter seasons are mainly limiting factors for productivity of fruits. Selecting and expanding cultivars for more yield and quality without attention to the rate of their frost resistance are impossible. In this research, the rate of relative resistance of some peach and nectarine cultivars at a sudden downfall of temperature was analyzed in vivo weather conditions during the 2008 winter season in Mashhad. The meteorology station of the studied area (Golmakan station) registered -22°C for the minimum temperature of this year on 13th January, which was a little different from the weather of Mashhad. This test is performed as factorials in the form of a randomized complete block design with three replications. Relative ionic leakage is used on reproductive and vegetative buds, annual and biennial wood in peach cultivars (J.H.Hale, Elberta, Redhaven, Mashhad Sorkhosepfide and Mashhad Ghermez) and nectarine cultivars (Indipendens, Qiuota, Nectared 6 and Stargold). Between studied peach and nectarine cultivars, Mashhad Sorkhosephid and Mashhad Ghermez cultivars, which are cultivars native to Iran, were the most resistant reproductive buds that did not have a significant difference in comparison with other peach and nectarine cultivars with the exclusion of Indipendense cultivar. The most sensitive reproductive buds were nectarine Indipendense cultivar and the most resistant vegetative buds were Mashhad Sorkhosephid and Redhaven in peach cultivars and Nectared 6 and Qiuta cultivars in nectarine cultivars. The relative ionic leakage of annual wood in peach cultivars was more than nectarine cultivars. But the relative ionic leakage between peach cultivars was not significant and Nectared 6 cultivar did not have any difference compared to peach cultivars. The "Sorkhosephid" peach cultivar of Mashhad had more solution carbohydrates in generative buds and annual wood (93.33 and 136.50 mg/lit, respectively) than other studied cultivars. Results of sugar rate followed relative ionic leakage and visual observations and showed increasing sugar and decreasing ionic leakage in organ caused lower their susceptibility to cold.
Key words: Peach and nectarine Cultivars, Chilling injury, Relative ionic leakage, Visual observation, Sugar
