hoda moradi; Majid Azizi; Vahid Rowshan; Hossein Arouiee
Abstract
Introduction: The genus Nepeta with the common Persian name “Pune-Sa” is one of the most important genera of Lamiaceae family. It has aromatic plants which are endemic of Iran. Its different species are distributed almost in all parts of Iran. Some of these species are valuable in medicine and used ...
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Introduction: The genus Nepeta with the common Persian name “Pune-Sa” is one of the most important genera of Lamiaceae family. It has aromatic plants which are endemic of Iran. Its different species are distributed almost in all parts of Iran. Some of these species are valuable in medicine and used for medicinal purposes. Nepeta glomerulosa Boiss. is one of the most medicinal species of this family. This study was conducted to examine seed germination characteristics and to evaluate cardinal temperature of seed germination of N. glomerulosa.
Materials and Methods: Seeds of N. glomerulosa were collected from Abade region of Fars province in the middle of September 2014. Treatments were including four levels of cold period (1, 2, 3 and 4 weeks at5ºC) and four levels of potassium nitrate (0, 0.1, 0.2 and 0.3 %). Factorial experiment with a completely randomized design in three replications was done, to evaluate seed germination percentage, germination rate, T50, mean germination time, root and shoot length, root and shoot fresh and dry weight. Also, the effects of 8 constant temperatures including 5, 10, 15, 20, 25, 30, 35 and 40 ˚C and 3 variable temperatures including 5/15, 10/20 and 20/30 ˚C (12h light/12h dark), was investigated to evaluate germination percentage, germination rate, mean germination time and cardinal temperatures of seed germination.
Results and Discussion: The results showed that cold period and potassium nitrate interaction was significant only on root length. Potassium nitrate had no significant effect on germination rate, but the germination rate was increased by increasing the duration of being at5 ˚C, so that the highest germination rate was observed in 3 and 4 weeks at5 ˚C (1.7 and 1.2 /day respectively). The lowest germination rate (1.0 /day) was observed in 2 weeks at5 ˚C treatment. The maximum shoot length (51.5 mm), shoot fresh weight (30 mg), shoot dry weight (1.3 mg) and root dry weight (0.6 mg) was obtained in 2 weeks at 5 ˚C treatments andpotassium nitrate 0.1% was also the best treatment for abovementioned traits. Among the treatments, 3 weeks cold period and 0.1 % potassium nitrate showed the most positive effect on seed germination characteristics of N. glomerulosa, although, had no significant effect on germination percentage.
Based on the results of the second experiment, temperature had significant effect (P≤0.01) on germination percentage, germination rate and mean germination time. The highest amount of germination percentage (76 and 77.3 %), germination rate (4.4 and 5 /day) and mean germination time (5.1 and 5.4 /day) were recorded in the range of 20 to 25 ˚C. Germination rate and germination percentage were substantially reduced by increasing temperature above 25 ˚C. The day and night variable temperatures had significant effect on seed germination percentage and germination rate of N. glomerulosa seeds. The highest amount of germination percentage (72 and 69.3 %) and germination rate (4.6 and 5.7 /day) were observed at10/20 and 20/30 ˚C. The results of the relationships between temperature and germination rate showed that base, optimum and maximum temperatures for seeds of N. glomerulosa were 15 ˚C, 22 ˚C and 46 ˚C, respectively.
Conclusion: The overall results showed that most chilling occurred under natural conditions during winter and early spring which caused increasing inseed germination of lots of plants. This also found that increasingin chilling period significantly improved the seed germination characteristics of N. glomerulosa. Also, treating with potassium nitrate had significant effects on germination properties of N. glomerulosa. Although applying potassium nitrate and cold period did not show interaction effects on seed germination.
The effects of different day and night temperatures on seed germination rate and percentage have been reported in many medicinal and aromatic plants as may be due to the similarity of these conditions to the natural conditions. Comparing cardinal temperatures of N. glomerulosa with the other species of this genus indicated that they have different cardinal temperatures. Many of researchers reported that germination cardinal temperatures are significantly different amongspecies and different genotypes of species. Determining of cardinal temperatures helps us to estimate temperature range of wild species in natural areas, planting time and also suitable areas for cultivation.
gonai baghdadi; Majid azizi; N. Sedaghat; V. Rowshan; H. Aroiee
Abstract
Introduction: The aim of medicinal plant storage is to preserve qualitative and quantitative properties of active substance. Carum copticum fruits (Zenyan in Persian) were used for its therapeutic effects. Seed storage condition after harvest till to extraction time is not suitable in our country and ...
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Introduction: The aim of medicinal plant storage is to preserve qualitative and quantitative properties of active substance. Carum copticum fruits (Zenyan in Persian) were used for its therapeutic effects. Seed storage condition after harvest till to extraction time is not suitable in our country and the major part of seed quality deteriorates during the storage period. So, the loss of seed qualitative and quantitative characteristics will increase during unsuitable storage condition. Appropriate storage conditions and management preserve seed active substance, seed viability and vigor for long periods by reducing the rate of seed deterioration. Optimal seed storage can be achieved by modifying the environment around the seeds. Numerous storage systems have evolved over the years for post harvest preservation of crop seeds. The aim of this study is to evaluate the effect of various storage conditions and storage time on essential oil percentage and germination factors in C. copticum seeds during the storage period. The results of this research will be used for optimum storage of these seeds to better preserve their quality.
Materials and Methods: In order to investigate the effects of storage conditions (packaging materials and temperature) and storage time on quality of C. copticum stored seeds, a split-plot factorial arranged in a randomized complete block design with three replications is performed in Faculty of Agriculture at Ferdowsi University of Mashhad during 2013 and 2014.Tukey's range test was performed to determine the significant difference between treatments. The calculations were conducted by JMP 8 software. Temperature) at two levels: 20±3°C and 30±3°C( as main plots and packaging materials (at six levels: paper, polyethylene, aluminum foil under vacuum condition, Polyethylene-polyamide packages under vacuum condition, Polyethylene-polyamide packages with a gas composition of [98%N2 + 2%O2] and [90%N2 +10%O2] ) and storage periods (at four levels: 0, 3, 6 and 9 months) as sub plots were considered. Seed samples were taken randomly from each package at four times period. Essential oil percentage, seed weight, gas composition in packages with modified atmosphere and seed germination factors (seed germination percentage (SGP), germination rate (GR), mean germination time (MGT), and germination Index (GI)) were evaluated during this nine months Storage.
Results and Discussion: The highest weight loss was 2/43% in the paper bags stored at 30°C at the fourth to sixth months especially in the fifth month. Aluminum foil package under vacuum condition stored at 30°C had the lowest weight and minimum weight changes during nine months of storage, so it was the best packages compared to others. The results show that Polyethylene-polyamide packages and Aluminum foil packages under vacuum condition are almost impermeable to air and moisture. Increasing weight at 20°C may be due to accumulation of water vapor from the respiration during early period of storage. Seed germination test provides an indication about seedling vigor as well as performance of seed in the field. In most cases, performance relates to the ability of seeds to germinate and produce a seedling that will emerge from the soil and development into a healthy vigorous plant. Packages with different combinations of gas (2% and 10% oxygen) at 30°C,aluminum foil under vacuum condition and Polyethylene-polyamide packages with a gas composition of [98%N2 + 2%O2] at 20°C were packages with higher germination percentage after nine months storage. Based on this results, it appears that packaging materials and storage temperature did not show any significant difference on essential oil percentage and further changes in the amount of oil related to duration of storage. Kumar et al. (2013) showed that the essential oil content and composition were affected by harvest time and storage conditions. Kazaz et al. (2009) investigated the effect of different storage temperatures (0◦C and 3◦C) and durations (7, 14, 21 and 28 days) on oil yield and essential oil components of oil rose (Rosa damascena Mill.). Their results showed that the effect of storage temperatures on oil content were not significant whereas the effect of storage duration was significant and it was similar to our results.
Conclusion: Essential oil percentage as the most important property of Carum copticum and germination percentage decreased significantly with increasing of storage period. Finally, results show that Polyethylene-polyamide packages with a gas composition of [98%N2 + 2%O2] and [90%N2 +10%O2] in 9 months storage time and 30±3°C storage temperature preserve qualitative properties better than other conditions. Whereas, aluminum foil package under vacuum condition and 20±3°C storage temperature are recommended for 6 month storage time.
H. Kaboli Farshchi; Majid azizi; Seyyed Hosein Nemati; V. Rowshan
Abstract
Introduction: Medicinal and aromatic plants can play an important role in commercial crops, which also represent a safe alternative for chemical pharmaceutical industries. St. John’s Wort (Hypericum perforatum L.) due to its therapeutic efficacy has been used for decades in folk medicine and is considered ...
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Introduction: Medicinal and aromatic plants can play an important role in commercial crops, which also represent a safe alternative for chemical pharmaceutical industries. St. John’s Wort (Hypericum perforatum L.) due to its therapeutic efficacy has been used for decades in folk medicine and is considered as a promising medicinal plant with valuable potential as a source of hypericin, essential oils and antioxidants. Studies on agronomic factors such as application of potassium and humic acid as well as nitrogen fertilization on yield, essential oil and antioxidant activity of Hypericum perforatum have not been investigated thoroughly until now. This study was designed to study the effect of using humic acid and potassium sulphate on morphological and phytochemical characteristics of Hypericum perforatum.
Materials and Methods: The plant material was prepared from Science and Technology Park in Khorasan-e Razzavi–Mashhad. In spring, the plants were transplanted into the field of Horticulture department, Ferdowsi University of Mashhad, for fertilizing treatments. The experimental layout was factorial in a complete randomized design (CRD), with three replications. Potassium Sulphate (K2SO4) was applied at the rates of 0.0 (K0), 60 (K60) and 100 (K100) kg ha-1. The other treatment were humic acid, which was applied at three rates 0 (H0), 20 (H20) and 40 (H40) L ha-1. Plants were treated at two stages, before flowering by potassium fertilizer and fertigated four times in 15-day intervals with humic acid. Besides the fresh and dry weight, number of flowers, stem height and number of flowering stems were determined at the end of the growing stage.
Results and Discussion: The ANOVA indicated that most of measured attributes of Hypericum perforatum plant were significantly affected by both treatments. Data presented revealed that the highest stem (91.6 cm) was recorded at the highest level of potassium sulphate (K100) treatment, while the shortest height (60.4 cm) was recorded at the lowest level of potassium sulphate (K0) treatment. On the other hand, the highest stem (80.33 cm) was achieved with the highest level of humic acid treatment. No significant difference was observed between the highest level of humic acid (H40) and the second level (H20), while both mentioned levels showed a significant difference with control (H0). This means that the control plants possessed the lowest stem height (66.7 cm). The DPPH method for evaluation of antioxidant activity reflects the ability of the present compounds to scavenge hydrophilic free radicals. DPPH inhibition was investigated and the results were evaluated as relative activities against α-tocopherol as positive control. A lower EC50 indicates a higher antioxidant activity. As it is shown, the least EC50 which indicates the highest antioxidant activity belonged to plants treated with potassium sulphate at (100 kg.ha-1). Control plants possessed the least antioxidant activity and showed no significant difference compared with plants treated with 60 kg.ha-1 of this fertilizer. Humic acid had a significant effect on antioxidant activity of H. perforatum. The lowest value of EC50 was related to the highest level of humic acid, while the highest value of EC50 was obtained from control plants. There was also significant difference between control and two other levels (20 and 40 L ha-1) of humic acid. Applied fertilizers at their high levels showed significant effects on EC50 decrease, which means the increment of antioxidant activity of H. perforatum. The high antioxidant activity of this medicinal plant is the reason of flavonoids and phenols existence. It seems that application of these fertilizers have led to increment of the mentioned secondary metabolites and followed by an increase of antioxidant activity in comparison with control plants. Totally, H. perforatum possessed a high antioxidant activity. Based on recorded data of the present study, application of the mentioned fertilizers separately or simultaneously caused the increase of this important medicinal plant fresh and dry weight, and finally the yield. Number of flowers, stem height and flowering stems reached the highest value at the highest level of both fertilizers (K100H40). As potassium plays an important role in the development of reproductive parts of plants, it might be one of the reasons of the above mentioned increase. The highest essential oil content was recorded at high levels of both fertilizers (K100H40). Since the highest essential oil content accumulates in the flowering stems, an increase of these stems height and a higher number of flowers would lead to an increase of essential oil content of H. perforatum. Antioxidant activity of this plant is flavonoids contributed. It seems that application of these fertilizers increased the antioxidant activity by an increase of this pigment content.
sonayye beigi; M. azizi; H. Nemati; V. Rowshan
Abstract
Introduction: Medicinal plants are rich in active substances and primarily have been used in the manufacture of many drugs. Basil (Ocimum basilicum L.) is one of the important medicinal plants whichbelongs to the Lamiaceae family. Basil essential oil content (between 0.5 to 1.5 percent) varies according ...
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Introduction: Medicinal plants are rich in active substances and primarily have been used in the manufacture of many drugs. Basil (Ocimum basilicum L.) is one of the important medicinal plants whichbelongs to the Lamiaceae family. Basil essential oil content (between 0.5 to 1.5 percent) varies according to climatic conditions of habitat location. Basilneeds a lot of water during growth period and it is very sensitive to water stress and shows wilting symptoms very soon after water shortage. Iran is located in an arid and semi-arid region which has little precipitation that is not enough for crop water requirements. Nowadays, the use of superabsorbent polymers is one of the ways to create sustainable agriculture and increase irrigation efficiency. They can store high water or aqueous solutions in root zone of plants and to reduce negative effects of drought stress. So, improvement of plant growth, increasing of irrigation intervals, reducing water loss and costs of irrigation is due to the application of superabsorbent polymers. Mucilages are also the herbal polysaccharides, soluble in water, and commonly include carbohydrates and can be used as hydrophilic polymers. The aims of this investigation were to study the effects of hydrophilic polymers on water use efficiency, morphological characteristics (dry matter, leaf area, and leaf number), essential oil quantity and yield of basil to harden plant to drought stress and to evaluate its potential to cultivate in arid regions. In addition, taking steps forward towards sustainable agriculture, by reducing the cost of agricultural production, helps protecting the environment.
Materials and Methods: This research was conducted as a pot experiment at the department of Horticultural Science‚ college of Agricultural‚ Ferdowsi University of Mashhad‚ Iran, during 2012-2013.The research was set out in a factorial experiment on the basis of completely randomized block design with three replications.Two hydrophilic polymer Stockosorb® (industrial) and malva leaf (herbal) with two application methods (mixed with soil, mixed with soil+root) at 4concentrations (0, 0.1%, 0.2% and 0.3% w/w) were used. Leaf number, leaf area, dry matter yield, water use efficiency, essential oil quantity and yield were measured. Improved seeds of Ocimum basilicum var. Keshkeniluvelou were sown and seedlings were transplanted to the pots in four-leaf stage. Hydrogels were prepared and mixed with water after weighing the polymers according to determined concentration. After establishment, all pots were irrigated with a determined amount of water after reaching to the highest concentration (0.3%) of the wilting point. So, in this state in addition to the treatment at 0.3% treatment, lower levels (0.2% and 0.1%)were reached to wilting point earlierand were exposed to water stress.
Results and Discussion: Superabsorbent polymers can by absorption of irrigation and rain water, prevent deep percolation of water and with decreasing irrigation interval and reduced water consumption and increase water use efficiency. The results showed that using Stockosorb superabsorbent polymer and malva leaf as herbal hydrophilic polymersare useful for water supply of the plant in water stress.Application of these substances on morphological characteristics, water use efficiency and basil oil, were superior to the control.According to the obtained results, malva leaf use in soil and roots in treatmentless than (0.1% and 0.2%)number and leaf area and higher than (0.3%)increased dry matter yield, water use efficiency and the essential oil quantity and yield. Stockosorb (0.2%) byboth applying methods increased dry matter yield and water use efficiency. Also Stockosorb (0.2%),soil application increased leaf number and area. The essential oil yield and quantity increasedwith use of Stockosorb in soil + root in for0.2% and 0.3% treatments, respectively.
Conclusion: Due to the importance of cultivation of medicinal plants to provide conditions to increase essential oil yield and quantity at the same time, the best method used were malva leaf and Stockosorb with concentrations higher than (0.3%) and soil application methodor the use of both polymers with application method in soil + root in the moderate concentration (0.2%).For that purpose, we obtained favorable conditions for improving the morphological characteristics, water use efficiency and increasing the quantity and of essential oil yield in drought stress simultaneously. The results showed that natural compounds can be a good alternative for chemical compounds, but further experiments are needed for their commercialization.
R. Ameri; Majid azizi; Ali Tehranifar; V. Rowshan
Abstract
In order to study the effect of natural antitranspirant compound and water stress on growth, development and essential oil content of Ocimumbasilicum a factorial experiment based on completely randomized design with three replicates was conducted. 3 levels of water stress (500 as control, 375 and 250 ...
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In order to study the effect of natural antitranspirant compound and water stress on growth, development and essential oil content of Ocimumbasilicum a factorial experiment based on completely randomized design with three replicates was conducted. 3 levels of water stress (500 as control, 375 and 250 ml/day) and 3 antitranspirant compound (chitosan, plantago mucilage and psyllium mucilage) in 3 levels of 0.5, 1 and 1.5% (m/v) and applied during the plant growth. Photosynthesis, transpiration, stomatal conductance, stomatal chamber CO2, leaf temperature, fresh and dry weight of herb, essential oil percentage and content were measured. The results showed that water stress and antitranspirant application had a significant effect on all measured traits (P≤0.05 and P≤0.01). The highest values of these traits were observed in control for water stress treatment and different levels of antitranspirant compounds. Transpiration levels from leaf were significantly decreased by antitranspirant compounds application. Chitosan (1 and 1.5%) decreased transpiration by 200% over control. Photosynthesis was also increased up to 30% by chitosan treatment (0.5 and 1) in comparison to control.Also, antitranspirant compounds increasing dry matter yield in water stress condition but reducing essential oil % and yield in comparison with control. In general, according to the result of this experiment, antitranspirant compounds with natural origin are safe, biodegradable, easy available, low cost and alternatives which can be used in substitution with common chemical types.
