Majid Azizi; Mansour Mashreghi; Fatemeh Oroojalian; Naser Shahyahmasebi
Abstract
To study the interaction of silver nanoparticles with antibacterial effects of Kelussia odoratissima and Teucrium polium essential oils consolidated trial based on calculation of MIC and FIC index or differential inhibitory concentration and simple method checkboard designed and implemented. Essential ...
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To study the interaction of silver nanoparticles with antibacterial effects of Kelussia odoratissima and Teucrium polium essential oils consolidated trial based on calculation of MIC and FIC index or differential inhibitory concentration and simple method checkboard designed and implemented. Essential oil of the plants extracted by hydro-distillation using clevenger apparatus. In this study, common food contaminant bacteria such as Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli O157: H7, Salmonella enterica and Ps. aureogenosa were used. The results showed that the antibacterial properties of the essential oil of Teucrium is stronger (MIC between 0.16 to 1.25 mg/ ml) than of the oil of Kelussia (MIC between 0.3 to 2.5 mg/ml). Antibacterial properties of silver nanoparticles (MIC between 0.006 to 0.025 mg) were stronger significantly. Calculate the differential inhibitory concentration index (FICi) implies the existence of synergistic and additive effect between silver nanoparticles and oil of both plant which will depend on the type of pathogen. This means that the combined use of silver nanoparticles and essential oil of the plant especially Teucrium essential oils strong inhibitory effect with lower concentrations of the essential oils was observed. Antagonistic interaction between silver nanoparticles and oil treatments in any of these two plants were observed.
Mansour Mashreghi; Majid Azizi; Fatemeh Oroojalian; Naser Shahyahmasebi
Abstract
In this research the essential oils (EOs) of Kelussia odoratissima and Teucrium polium were extracted by hydrodistillation. Extracted essential oils constituents were analyzed by gas chromatograph (GC) and GC/mass spectrometry and the essential oils constituents identified according to retention time ...
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In this research the essential oils (EOs) of Kelussia odoratissima and Teucrium polium were extracted by hydrodistillation. Extracted essential oils constituents were analyzed by gas chromatograph (GC) and GC/mass spectrometry and the essential oils constituents identified according to retention time and mass spectrum. Then minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the essential oils against Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes، Escherichia coli O157H7, Salmonella enterica, and Pseudomonas aureogenosa were determined by microdilution technique using ELISA reader. The results showed that there are differences between the essential oils constituents as the main constituents in Kelussia odoratissima were (Z)-ligustilide, (Z)-3-butylidene-phthalide, limonene+-phellandren B. The main constituents of Teucrium polium essential oils were β-caryophylene, Germacrene D, γ-cadinene, (Z)- nerolidol, camphor, β-pinene, α- camphene, linalool, α-humulene. The MIC of Kelussia odoratissima EO was between 0.31 mg/ml (for S. aureus) to 2.5 mg/ml (for Salmonella enterica) but MIC of the Teucrium polium EO was between 0.16 mg/ml (for S. aureus) and 1.25 mg/ml (for Salmonella enterica). In conclusion, indigenous medicinal plants could be used for effective control of food borne pathogens as a complementary method that has less unfavorable effect on organoleptic attitudes of each products
