Shadab Faramarzi
Abstract
Introduction: Ziziphus mauritiana is a plant from the family of Rhamnaceae. The fruits of Indian jujube contain high amounts of soluble sugars, non-volatile acids and phenolic compounds. Antioxidant properties and cytotoxic effects of the fruit, bark and seed of the Indian jujube has been reported. Also, ...
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Introduction: Ziziphus mauritiana is a plant from the family of Rhamnaceae. The fruits of Indian jujube contain high amounts of soluble sugars, non-volatile acids and phenolic compounds. Antioxidant properties and cytotoxic effects of the fruit, bark and seed of the Indian jujube has been reported. Also, it have been proven the effects of Indian jujube seed on insomnia and anxiety. Several studies have been conducted on genetic diversity based on morphological traits and molecular markers. This research was conducted to investigate the morphological traits including fresh weight, fruit length, fruit diameter, length and diameter of seed, firmness, total soluble solids (TSS), total phenol content (TPC) and antioxidant capacity of 11 Indian jujube varieties are located in Minab agricultural research station (longitude 57˚ 05, latitude 27˚ 06).
Materials and Methods: This experiment was conducted in a randomized complete block design (RCBD). Fruits were selected from trees in Minab agricultural research station and transferred to the laboratory of Minab education center. Some of fruits were also transferred to the laboratory of TarbiatModarres University and were liophylized in the Freeze-Dayer. The traits included fruit weight, length and diameter of the fruit, total soluble solids (TSS), tissue firmness and length and diameter of the seed were measured. Due to the abundant sugar content of the fruit, extraction was carried out using liquid-liquid method and with two solvents: ethyl acetate and distilled water. Total phenol content (TPC) was measured by Folinciocalteu in terms of mg of equivalent gallic acid/g of fruit dry weight. The phenolic compounds extracted was used to determine the antioxidant properties of the fruit. Scavenging of ABTS radical was calculated by spectrophotometry method at 734 nm wavelength.
Results and Discussion: The results showed that the fresh weight of the fruit was between 10.57 (Z3) and 23.66 (Z11) grams. In a study on 22 genotypes in Pakistan, fruit weight varied from 6.8 to 37.64 grams. The highest amount of fruit length to diameter ratio was seen in the genotype Z11 with a value of 5.76. For firmness parameter, the highest value was related to Z2. The highest amount of TSS was found in Z6 and Z9 genotypes (20.69 and 20.01 ˚Brix, respectively). The results of using two solvents for TPC extraction, had different results in studied genotypes. TPC extracted with ethyl acetate solution was highest in Z11 with 80 mg equivalent gallic acid / g of fruit dry weight, and then Z10 (70), Z5 (65), Z7 (60) and Z1 (55) genotypes were in order. The lowest amount of TPC was observed in Z2 with 38 mg equivalent gallic acid / g of fruit dry weight. The highest amount (45) of TPC extracted with water solvent was observed in Z10. In previous studies, it has been shown that the fruit of Indian jujube has high soluble sugars and high phenolic compounds. 12 phenolic compounds have been identified by HPLC from Indian jujube’s fruit, including caffeic, p-hydroxybenzoic acid, ferrolic acid and p-cumaric acid. The total phenol content and total flavonoid content was between 453.3 to 1298 mg equivalent gallic acid / 100 g of dry weight for seven Z. jujube species. Radical scavenging of ABTS˚ was different in ethyl acetate extracted solution in the studied Indian jujubes. The highest amount of ABTS˚ absorption was observed in the concentration of 40 ug of ethyl acetate extracted solution, in Z7 and Z11 genotypes, and the lowest radical inhibitory was seen in Z8. The highest IC50 in water and ethyl acetate solvents was related to Z6 and Z8 genotypes, respectively.
Conclusions: We can conclude that there was a significant morphological diversity in fruits of studied genotypes. In this study, genotype Z11 had the larger fruit than others (23.66 g), with the highest ratio of length to diameter. In a study on Indian jujubes in Pakistan, the highest fruit weight was 37.64 g. TSS in Z6 and Z9 genotypes was the highest amount (20 ˚Brix), and TSS in previous studies has been reported between 11 to 18. TPC and antioxidant capacity were different in all genotypes based on the used solvent (water and ethyl acetate). Therefore, different solvents can effect on the amount of phenolic content and radical scavenging properties. But, the highest TPC extracted with ethyl acetate solvent was observed in Z11 with 80 mg equivalent gallic acid / g of fruit dry weight, and the lowest amount was observed in Z2 with 38 mg equivalent gallic acid / g of fruit dry weight. From antioxidant capacity point of view, the highest amount of ABTS˚ absorption was observed in ethyl acetate extracted solution of Z7 and Z11 genotypes.
Shadab Faramarzi; Abbas Yadollahi; Ghasem Karimzadeh
Abstract
Introduction: Apple (Malus Miller) belongs to Rosacease family and the subfamily of Pomoideae. This fruit is at first place among fruits of temperate zones. The cultivated apple (Malus × domestica Borkh.) is a complex hybrid of the apple species. Chromosomal basis of this subfamily are x = 17 and the ...
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Introduction: Apple (Malus Miller) belongs to Rosacease family and the subfamily of Pomoideae. This fruit is at first place among fruits of temperate zones. The cultivated apple (Malus × domestica Borkh.) is a complex hybrid of the apple species. Chromosomal basis of this subfamily are x = 17 and the ploidy levels have been reported for diploid (2n=2x = 34), triploid (2n =3x= 51) and tetraploid (2n= 4x= 68). Since Iran is close to the apple diversity region (Central Asia), it has a good variation of apple varieties. Despite the high levels of variation in apple cultivars and species in Iran, there is not still a database of genome size. Classification of plants according to their genome size, especially at lower taxonomic levels is important for breeders. Over the past years, several methods for estimation of nuclear DNA content (genome size) was common, but recently, the use of flow cytometry (FCM) has been increasingly used. Flow cytometry is the best method to estimate DNA c-value and ploidy levels in apples. In this study, DNA c-Value and ploidy level of Iranian apple varieties has been estimated by flow cytometry and propidum iodide staining.
Materials and Methods: Fully expanded young leaves of all apple varieties were collected in the summer 2013. Nuclear extraction was performed using Partec kit as following: 1 cm2 apple leaf and 1 cm2 parsley leaf (as internal standard) were co-chopped with razor blade after adding 500 µl of nuclear extraction buffer. Then, the extract was filtered by two kind of filters (50 and 30 um). One ml of staining buffer, 4 µl of RNAase and 4 µl ofpropidium iodide was added for 15 min at room temperature. Finally, nuclei were counted using flow cytometry (BD FACSCanto II, USA) at Tarbiat Modarres University. The genome size was estimated according to bellow formula:
DNA 2C-value sample =
Also, given the high levels of phenolic compounds in apples, treating with PVP and PVP 1% were performed to evaluate the effect of phenolic compounds on estimation of genome size. Finally, Histogram analysis and DNA c-value estimation were done with Partec Flow Max software. The difference between means was obtained by SAS software ver. 9.2 and LSD tests.
Results and Discussion: The results showed that genome size obtained from Partec Flow Max software and ranged from 1.57 pg for ‘Golab- Bastam’ to 1.73 pg for ‘Golab- Kermanshah’. Histogram analysis was demonstrated that all studied cultivars are diploid. The average genome size in this study was 1.62 pg. Research conducted on foreign apple varieties have showed that the genome size of diploid species from was obtained 1.45 for M. fusca to1.68 pg for M. ransitoria. The genome size for triploid species was ranged from 2.37 to 2.57 pg. In this study, genome size was calculated in terms of mega base pairs and was different from 748 Mbp in ‘Golab- Bastam’ to 846 Mbp in ‘Golab- Kermanshah’. Thus, the size of the genome was closed to M. ransitoria (1.68 pg). This species is native to China, which is a Crab apple and used as an ornamental tree. It has been reported that Iranian apple are M. domestica Borkh. In another study, genome size was identified in the range from1.245 pg for diploid species of M. tschonoskii to 1.653 pg for M. florentina. M. florentina species is native to Balkans and Italy, that is an ornamental tree and its genome size is close to M. domestica Borkh. (1.653 pg).
Conclusion: Classification of plants according to their genome size seems to be important, especially at lower taxonomic. Genome size, even in very close species can also be different, for example, northern corn with more heterochromatin has larger genomes than those who are located in south (less heterochromatin). This study appears the variation of DNA 2C-value in Golab cultivars, even though Golab cultivars are known clones with low genetic diversity. Therefore, it is likely that Iranian apple varieties, with the same ploidy level, have been had difference in genome size. There are various ploidy level in apple, including diploid (2n = 34), triploid (2n = 51), tetraploid (2n = 68) and hexaploid (2n = 102). Thus, it is expected that current apple M. × domestica Borkh., have been contributed some several species such as M. prunifolia (Willd.) Borkh., M. baccata (L.) Borkh., M. sieboldii (Regel) Rehder, M. sylvestris, ،M. orientalis Uglitzk and M. sieversii.
