Medicinal Plants
Fatemehzahra Amirmohammadi; Majid Azizi; Seyyed Hossein Nemati
Abstract
IntroductionThe Nepeta is one of the largest genus of Nepetoide subfamily, in the Lamiaceae family with about 79 species in Iran. Most of the species are perennial rarely annual with different vegetative forms, leaves covered with trichome. Glandular trichomes are widely distributed all over the aerial ...
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IntroductionThe Nepeta is one of the largest genus of Nepetoide subfamily, in the Lamiaceae family with about 79 species in Iran. Most of the species are perennial rarely annual with different vegetative forms, leaves covered with trichome. Glandular trichomes are widely distributed all over the aerial part of Nepeta species, and their largely to its great importance and their structures can vary widely among plants species. Some species contain monoterpenes, sesquiterpenes, cyclopentanoid iridoids derivatives and nepetalactones which they are used in folk medicine as, diaphoretic, diuretic, antitussive, febrifuge agent. In the present work, scanning electron microscopy (SEM) was used to determine the nutlet micromorphology and also micromorphology and distribution of the glandular trichomes of Nepeta binaludensis “an Iranian endemic species” and Nepeta cataria in order to improve the knowledge of the species and to evaluate the usefulness of this feature for phytochemical and systematic purpose. The chemical composition of its essential oil was also analyzed. This paper provides a detailed description of trichome morphology and nutlet in Nepeta binaludensis andNepeta catariaspecies growing in Iran, by scanning electron microscopy (SEM), and evaluates the systematic significance of such characteristics. Materials and MethodsThe research was conducted in research garden of Agricultural Faculty of Ferdowsi University of Mashhad during 2017. Seeds of two Nepeta species were provided by Institute of Plant Sciences in Ferdowsi University of Mashhad.The seeds were cultivation in greenhouse and the seedling (10 cm long) were transplanted in the field. During the summer aerial parts of the plants were harvested at flowering stage, dried in shade, and (30 g) were hydro distilled for 3 h using Clevenger apparatus. For SEM study, leaves were fixed with 3% glutaraldehyde in 0.1 M sodium phosphate buffer, pH 7.2 for 30 min at 4 °C. After washing in water the material was dehydrated through an ethanol gradient and critical point dried. Specimens were mounted onto SEM stubs using double-sided adhesive tape and coated with palladium. For nutlets observation, seed were directly mounted onto aluminum stubs using double-sided adhesive tape and coated with palladium and photographed. ResultBased on the obtained data, the surface leaves of both species (N. binaludensis and N.cataria) was observed forglandular and non-glandular trichomes distribution. (The glandular trichomes of the peltate types). Trichome density and size was variable as 7-21mm2 leaf surface (63-77 µm) in N. binaludensis and as 20-30 mm2 leaf surface (47-67 µm) in N.cataria. In both species only one types of glandular trichome was identified. Peltate trichomes comprise of a basal cell situated in the leaf epiderm with a four-celled secretory head. Nutlets of Nepeta were blackish-brown or brown in color. One type surface ornamentation, can be distinguished: smooth-type. The nutlet ornamental pattern was smooth type with finely granulated in N. binaludensis and reticulate pattern in N.cataria species. Water-distilled essential oil of the aerial parts of two speciescultivated in field, were analyzed by GC and GC/MS. In essential oil of N.binaludensis and N. cataria, 51 and 26 components were identified, respectively. The main components of the essential oil of N. binaludensis were 1.8-cineole (14.8%), β-Pinene (10.4%) and p-Cymene (9.8%). Main compounds of the essential oil of N. cataria were 4a-a, 7-a, 7a-b-Nepetalactone (72/76%) and 4a-a, 7-a,7a-a-Nepetalactone (17.86%). Depending on the composition of key compound identified in essential oils, Nepeta species have been divided in two groups. Conclusions Depending on the composition of main compound identified in the essential oils, Nepeta species have been divided in two groups. On the basis of our research N. binaludensis belong to 1.8-cineole chemotype and of N.cataria belong to the nepetalactone chemotype. The leaves of N. binaludensis, N.cataria have be glandular and non-glandular trichomes. One types of glandular trichomes are identified: Peltate trichomes.Trichomes are distributed at the surface of leaves, having various functions and are extremely variable in the plants, plant organs location, density, form, therefore their morphology and structure could be of taxonomical importance in plant. Glandular trichomes contain or secrete substances which are widely used in pharmaceutical and cosmetic as well as in pesticide industry. The variation in the structure, morphology, density, secretion and function is noticed among these trichomes distributed on different plant organs. Nutlet surface can be useful as a taxonomic characteres.
Postharvest physiology
Vahid Ajami; Seyyed Hossein Nemati
Abstract
Introduction Watermelon Citrullus lanatus (Thunb) is known as a rich source of various vitamins (such as vitamin A) and phytochemical compounds that have high antioxidant activity. Studies show that characteristics such as taste, nutritional value, cost, and convenience in consumption have a direct impact ...
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Introduction Watermelon Citrullus lanatus (Thunb) is known as a rich source of various vitamins (such as vitamin A) and phytochemical compounds that have high antioxidant activity. Studies show that characteristics such as taste, nutritional value, cost, and convenience in consumption have a direct impact on consumer choice, therefore, in recent decades, the desire to consume and buy cut products has been increasing. Also, due to the large size of the watermelon, to avoid waste, this product can be offered sliced. Damage caused by cutting not only increases respiration and ethylene production, but also increases other biochemical reactions that are responsible for changes in color, taste, and aroma, as well as in the texture and quality of nutrition. Also, cutting the products and removing the natural covering of the fruit creates the conditions for increasing the microbial load. In order to increase the natural resistance of fruits and vegetables as well as maintain the sensory and nutritional quality of fresh products, it is recommended to use environmentally friendly technologies such as salicylic acid (SA). Citric acid, like salicylic acid, is considered a safe compound, and as an organic acid, it can be used as an approved food additive.Due to the positive effects of salicylic acid and citric acid on the quality properties of fresh products, no information has been found regarding the post-harvest use of these compounds on sliced watermelon. Therefore, in the present study, our aim was to investigate the effects of citric acid and salicylic acid treatments on sensory properties, quality, microbial load and color changes of cut watermelon fruit during the storage period. Our findings can provide a new strategy for maintaining the quality of sliced watermelon fruit.The microbial load and fruit tissue softening resulting from fruit cutting lead to a decline in quality due to increased fruit respiration and water loss, which are limiting factors for the post-harvest shelf life of cut watermelon fruits. In the present study, the impact of citric acid and salicylic acid on some quality indices of cut watermelon pieces during the storage period was investigated. Materials and Methods The harvested fruits were transported to the laboratory and their external surface was disinfected with sodium hypochlorite (200 μL/L) and the skin of the fruits was removed by a sharp and sterile knife. The harvested fruits were separated from their peels, and then the fruit flesh was cut into 4 cm by 4 cm pieces. The pieces were immersed in salicylic acid solutions (1 or 2 mM) and citric acid solutions (0.5 and 1 mM) for 2 minutes. Immersion in water was also introduced as a control. Subsequently, the fruits were packaged in polyethylene coverings and stored for 14 days at a temperature of 4 degrees Celsius. Results and Discussion Based on the results of variance analysis, immersion of freshly cut watermelon fruits in different concentrations of citric acid and salicylic acid had a significant effect on the firmness of the fruit tissue at the 1% level. The interaction effects of measurement time and immersion in solutions were significant at the 1% probability level. The highest amount of tissue stiffness was related to the treatments of 1 mM citric acid (4.58 newtons) and 2 mM salicylic acid (4.69 newtons), and the lowest value was obtained from the control samples (3.54 newtons). The highest weight loss was related to the control samples and the lowest amount was obtained from 1 mM citric acid and 2 mM salicylic acid.During the maintenance period, the highest and lowest weight loss was obtained from the control and 2 mM salicylic acid treatments, respectively. The highest amount of soluble solids was obtained from the control treatment and the lowest amount was obtained from the 2 mM salicylic acid treatment. The highest amount of microbial load was obtained from the control samples (6.11), and the lowest amount was obtained from the 2 mM salicylic acid treatment (4.02), followed by the 1 mM salicylic acid treatment (4.17). Also, with the passage of storage time, the amount of microbial load increased significantlyThis study was conducted with the aim of investigating the effect of salicylic acid and citric acid on the quality and microbial characteristics of cut watermelon. This experiment included the use of different concentrations of salicylic acid and citric acid to evaluate their effect on quality parameters and microbial load in watermelon slices. Quality characteristics, including color, firmness, sweetness and overall visual appeal, were measured using standard methods. In addition, the microbial load, including both bacterial and fungal populations, was determined to evaluate the antimicrobial potential of the applied acids. The results showed significant effects of salicylic acid and citric acid on increasing some quality traits and reducing microbial contamination in watermelon slices. This research provides valuable insights into the use of salicylic acid and citric acid as potential agents to improve the quality and safety of cut watermelonConclusions The results indicated that salicylic acid at both concentrations (1 or 2 mM) and citric acid at 1 mM led to a significant reduction in microbial load and weight loss. Moreover, the mentioned treatments restrained the increase in soluble solids content resulting from the post-harvest handling of cut watermelon fruits, contributing to the preservation of fruit tissue strength. The results of evaluating color indices and organoleptic properties indicated that salicylic acid treatments at both concentrations (1 or 2 mM) and citric acid at 1 mM preserved the fruit quality to the best extent. And the best treatment included the application of salicylic acid with a concentration of 2 mM. In general, among the treatments used in this experiment, 2 mM salicylic acid yielded the best results in preserving the quality of cut watermelon fruits during cold storage. Subsequently,
Ornamental plants
Toktam Oraee; Mahmood Shoor; Ali Tehranifar; Seyyed Hossein Nemati; Atiyeh Oraee
Abstract
Introduction Climate change predictions indicate that drought and extreme heatwaves will become more frequent and extreme in many regions. Drought is the main abiotic stress that severely reduces plant yield across the globe. Thus, this may have negative consequences for the agricultural soils, ...
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Introduction Climate change predictions indicate that drought and extreme heatwaves will become more frequent and extreme in many regions. Drought is the main abiotic stress that severely reduces plant yield across the globe. Thus, this may have negative consequences for the agricultural soils, as it limits the availability of water and nutrients to soil microorganisms and plants that develop on these soils. To cope with this situation, the use of organic amendments is the best option. Recent studies have shown that the application of organic fertilizers can affect soil moisture and thus, mitigate the negative effect of climate change on that parameter. Organic amendments increase soil organic matter content thus improving soil physical, chemical, and biological properties, and therefore, can confer drought resistance to soils. The application of organic residues has been presented as a great strategy against soil degradation in semiarid environments. However, the interactions between organic amendments and drought in hollyhock plants are not fully known. Here, we evaluate whether the organic amendment influences the physiological traits of hollyhocks and soil properties under drought conditions. Materials and Methods The experiment was conducted in the research field at Ferdowsi University of Mashhad, Iran. The experiment consisted of three factors (cultivars, organic amendments, and drought) with organic amendments and drought having four and three levels, respectively. Drought treatments were controlled by a TDR at 80, 60, and 40% FC. The three sources of organic amendments were used cow manure, rice hull and wheat straw. Seeds were planted in cocopeat, perlite, and peat mixture trays in the greenhouse with an average temperature of 20 °C and under a photoperiod of 14 hours of light and 10 hours of darkness with a light intensity of 400 μmol-1 m2. In the 5-6 leaf stage, seedlings were transferred in pots (18 cm high and 8 cm in diameter) containing field soil. The plants were transferred to the field with four different substrates (field soil, field soil + manure, field soil + rice hull, and field soil + wheat straw) and were exposed to drought stress for one month during the flowering stage. This analysis examined both the physical and chemical properties of the soil, including changes in the macroelements nitrogen, phosphorus, and potassium. Results and Discussion Soil nitrogen changes were significantly affected by the interactions of ecotype with drought, ecotype with the medium, and drought stress with medium. The highest nitrogen changes were recorded in Mashhad ecotype under 80% FC. Nitrogen content in amended soil had an increasing trend during the experiment, but the amount of nitrogen had a decreasing trend in soil. In all media under stress, the amount of soil phosphorus was increased and the highest amount of phosphorus was observed in soil + manure at all irrigation regimes. The highest amount of potassium in both ecotypes was observed in soil + manure. The amount of potassium in amended soils under stress significantly increased, but in all organic amendments with increasing drought stress from 80 to 40% FC, the potassium content decreased. In both ecotypes, soil salinity was increased in all culture media. Han et al. (2016) stated that the amount of nitrogen, phosphorus, and potassium in the amended soil was higher than the substrates containing chemical fertilizer. The medium EC was alkaline at the end of the experiment and the salinity of the culture medium increased compared to the control. This study revealed a negative correlation between electrolyte leakage and dry weight in hollyhock plants. Furthermore, all measured physiological and growth parameters were significantly affected by the treatments. Notably, the Mashhad ecotype grown in soil supplemented with animal manure at 80% field capacity (FC) exhibited the highest levels of physiological traits (SPAD and relative water content) and growth index (dry weight).The application of manure + soil by providing macro elements reduces the negative effects of drought stress. Conclusion The type of crops grown in arid and semi-arid regions should be reconsidered. Also, some plants with high water requirements should be replaced with plants with low and unexpected water requirements. Because hollyhocks are low-expected plants that grow well in drought areas, so they can be considered as suitable species for cultivation in low-input systems and can tolerate drought situations by 40% FC in amended soil.
Medicinal Plants
Alireza Moshrefi-Araghi; Seyyed Hossein Nemati; Mahmood Shoor; Majid Azizi; Nasrin Moshtaghi
Abstract
Introduction Mentha is one of the most important genera of aromatic plants which belongs to the Lamiaceae family. The genus of Mentha is distributed across Asia, Africa, Australia, Europe, and North America. Mentha longifolia L., also known as wild mint, is a fast-growing aromatic perennial herb. ...
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Introduction Mentha is one of the most important genera of aromatic plants which belongs to the Lamiaceae family. The genus of Mentha is distributed across Asia, Africa, Australia, Europe, and North America. Mentha longifolia L., also known as wild mint, is a fast-growing aromatic perennial herb. It is widely used as herbal medicine and is beneficial for the immune system and fighting with secondary infections. The essential oil of this plant is partly responsible for the decongestant, antispasmodic and antibiotic effects. Currently, much emphasis is being laid on conserving plant germplasm as valuable bio-resources. Selection between and within accessions for a high level of herbage yield and other characters requires an effective tool to be applied by mint breeders. Achieving to cultivars that are more capable of optimum producing is a breeding goal. The objectives of this study were to analyze the diversion of the agronomical traits of Mentha longifolia L. genotypes from different regions of Iran under a similar condition in order to find the superior genotypes and introduce for the domestication of this plant. Material and MethodThis research was performed at the research field of the Ferdowsi University of Mashhad. The field is located at 36˚15' North latitude and 59˚38' East longitude, at an altitude of 985 meters. The information related to temperature and precipitation was obtained from climate station. Soil sampling was done in the depth of 0.3 meter and physical experiments on samples were done before starting the experiment. The field was fertilized by 25 kg/ha animal manure. Seeds of 20 genotypes of M. longifolia L. were prepared from Gene Bank of Research Institute of Forests and Rangelands and a view of the wild mint genotypes distribution was presented on the map. The experiment was performed in a field with 500m2 areas in 2015-2016 growing seasons. The experiment in form of compound analysis arranged in complete randomized design with three replicates of 20 wildmint's genotypes in every replication. In each furrow, fourteen bush was totally studied, in which the distance between the two plants was 20 cm. The plot was considered 1.2 by 3 m and the distance between rows was 0.50 m. The dimension in the plots for every replication area was 3.6 m2 and the distance between blocks were 1.5 and between experimental units were 0.5 meters. Picking up was done after elimination of 0.5 meters from every side of replication. The essential oil was extracted using Clevenger type apparatus and by hydrodistillation. Results and DiscussionAnalysis of variance showed that the effect of the diversity among the genotypes on morphological, agronomical and essential oil yield parameters of wild mint was significant (P≤0.01). The results of this study showed that Mentha longifolia L. herb has a high diversity under the same culture conditions among genotypes collected from 20 regions of Iran. Correlation result shows that vegetative organs have more effect on the essential oil content than the reproductive organs. This may be due to the distribution of essential oil accumulation and storage sites, which requires further research in this regard. The study of vegetative and reproductive characteristics suggests that the genotypes of the dry and semi-arid climates have different conditions, which may causes the separation of their genotypes compared with the other genotypes. The bi-plot, based on PC1 and PC2, reflects the relationships between the studied genotypes. The results of bi-plot of cluster analysis confirmed that genotypes were divided into two main groups based on agronomical and morphological traits. Conclusion Finally, plants of genotype G13 belongs to Hormozgan province and then G16 genotype belongs to Khuzestan province were selected because of more biomass, more aerial part volume and also the most essential oil performance compared to the other genotypes. Desirable traits for the aerial part such as the time of flowering, leaf length, leaf width, plant height, the highest number of leaves in the stem and internode distance are important traits that should be considered. In addition, the cultivation of these genotype in order to the further production can be the great help in the domestication of this species and, given the fact that the diversity is a precursor of breeding, this research can be an introduction for future breeding operations. However, further research is needed to confirm the phytochemical superior genotypes.
Growing vegetables
Leyla Cheheltanan; Saeid Khosravi; Seyyed Hossein Nemati
Abstract
IntroductionGrasses are narrow-leaved plants that are used as cover plants in landscape. These plants are one of the basic and necessary components of the green cover of most gardens, parks and as the background color of landscape. In Iran, due to the high costs of planting and management of grass, high ...
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IntroductionGrasses are narrow-leaved plants that are used as cover plants in landscape. These plants are one of the basic and necessary components of the green cover of most gardens, parks and as the background color of landscape. In Iran, due to the high costs of planting and management of grass, high water requirements, climatic incompatibility and damage to water and soil salinity, it is recommended to remove from the green space in some cities, especially in areas with low water and water and soil saline. If it is possible to benefit from the role and influence of these plants by observing the technical points and choosing the best species for each area. Salinity stress is the second limiting factor for the growth of plants in the world after drought, which affects the efficiency and performance of plants. Increase in salinity causes a decrease in the water potential in the soil. In this condition, the plant spends most of its energy to maintain the water potential, cell mass, and water absorption to have minimal growth. The aim of this research is the effect of external application of glycine betaine on the accumulation of osmolality compounds and the antioxidant system of sports grass under salt stress. Materials and MethodsThis research was done in factorial form in completely randomized design with 3 replications on Rashid variety cucumber. The factors included 4 levels of temperature (25, 30, 35 and 40 degrees Celsius) and 3 levels of salicylic acid (0, 0.5 and 1 mM). When the seedlings reached the two-leaf stage, they were sprayed with different concentrations of salicylic acid two times with an interval of five days. One week after the application of salicylic acid, temperature treatment was gradually applied. After applying each heat treatment, the corresponding pots were transferred to the greenhouse with a temperature of 25 degrees Celsius. Then, fresh and dry weight of root and shoot, leaf surface, chlorophyll index, electrolyte leakage, proline, polyphenol oxidase enzyme activity and superoxide dismutase enzyme activity were measured. Results and Discussion The results showed that salinity stress decreased all the study morphological, physiological and biochemical parameters including plant height, shoot fresh and dry weight, number of tiller, leaf area, chlorophyll content, protein and total antioxidant capacity in the studied plants. It also increased peroxidase enzyme, H2O2 and proline in plants, but glycine betaine application significantly improved the morpho-physiological characteristics of plants compared to the control under salt stress conditions. Thus, the highest height, shoot fresh and dry weight, leaf area, number of tiller, chlorophyll content, and protein and antioxidant capacity were observed in plants sprayed with glycine betaine. Also, the highest content of glycine betaine and activity of catalase and peroxidase enzymes and the lowest content of glycine betaine and H2O2 were observed in in plants sprayed with glycine betaine and 10 mM glycine betaine was more effective than 5 mM. The occurrence of salinity in plants disrupts the absorption of ions and causes the reduction of nutrients and increases sodium ions. One of the effects of salinity in plants is the reduction of photosynthetic activity, which results in the reduction of chlorophyll, carbon dioxide absorption, photosynthetic capacity, plant height, shoot fresh and dry weight, number of tiller and leaf area. One of the most strategies to deal with stress is accumulation of osmolyte and increasing the antioxidant activity, which makes plants resistant to environmental stresses. Salinity, through the toxic effect of Na+ and Cl- ions, affects the growth and performance of the plant by reducing the soil water potential, disrupting water absorption and imbalance of nutrients in the plant. The results obtained from comparing the average results of glycine betaine show that glycine betaine increased plant height, shoot fresh and dry weight, number of tiller, leaf area, chlorophyll content, total protein and antioxidant capacity, but on the other hand, it increased proline and H2O2 decreased, which is due to the accumulation of glycine betaine as a protector in plants under salt stress conditions. In stress conditions, glycine betaine can protect photosynthetic activities including photosynthetic enzymes, proteins and lipids in thylakoid membranes in the combination of photosystem II, and also the task of protecting cell membranes against osmotic stresses in the plant. ConclusionThe results obtained from this research showed that salinity stress reduced all the morphological, physiological and biochemical characteristics in the sport grass plants, but glycine betaine application played a positive role in reducing salinity damage and maintaining plant quality. Glycine betaine is known as one of the effective molecules in stress signaling, so it can protect the plant cells against stress by reducing the destruction of the membrane and by increasing the salt tolerance mechanisms. Also, glycine betaine 10 mM is introduced as the best treatment to reduce salinity damage in sport grass during present study.
Ornamental plants
Narmin Alavi Dehkharghani; Seyyed Hossein Nemati; Seyyed Majid Zargarian
Abstract
Introduction
As the population grows and cities expand, the demand for more green spaces has also increased. Grass is widely recognized as one of the most important ground covers in the world. However, the development of green spaces is hindered by a severe shortage of water resources and challenges ...
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Introduction
As the population grows and cities expand, the demand for more green spaces has also increased. Grass is widely recognized as one of the most important ground covers in the world. However, the development of green spaces is hindered by a severe shortage of water resources and challenges associated with managing and maintaining grass. The composition of trees and plants may cause to inhibitory or stimulation effects on crops, called allopathic effects. Therefore, understanding the allopathic effects is important to determine appropriate methods of cultivation and product management to prevent interference of allopathic substances in the growth and production of products.
Materials and Methods
This study was performed in two separate experiments as a factorial based on completely randomized design at Ferdowsi University of Mashhad. In the first experiment, the effect of three different levels of plant residue powder (zero, 0.46 and 1 g) of three types of trees (Pine, Silk and Chinaberry) on germination and growth of Lolium prenne and Festuca arundinaceae grasses with four replications in the greenhouse was investigated. The second experiment, the effect of 5 ml of aqueous extract prepared from plant residues of the mentioned trees on germination and growth of the mentioned grasses in three replications in the laboratory was investigated. At the end of both experiments, some of morophology traits (weight, length, germination index) were measured. Minitab software was used to analyze the data obtained from both experiments.
Results and Discussion
According to the analysis of variance table of the first experiment, the results show that the simple effect of grass treatment on plant and root length, fresh weight, germination percentage and rate are significantly different. In the simple effect of treatment the quantity of plant residues, all traits have a significant difference. Also, in the simple effect of treatment of plant residue kind, plant length, root length, fresh and dry weight, germination percentage and rate were significantly different. Interaction grass and the quantity of plant residues treatment and the interaction of grass and kind of plant residues treatment, all measured traits had a significant difference. The interaction triple treatments plant length, root length, dry weight, germination percentage and rate differences were observed. Therefore, in the interaction triple treatments, the most and least plant lengths were observed related to the treatment of Lolium grass with 0.46 gr of Pine residue powder and the treatment of Festuca with 1 gr of Chinaberry residue powder. Also, the most and least root lengths were related to Lollium grass with 0.46 gr of Pine plant residue powder and Festuca grass with 1 gr of Pine plant residue powder. In the triple of dry weight the most amount was related to Festuca with 1 gr of Chinaberry residue powder and the least was related to Lolium control. The most and least germination percentages belonged to the control Festuca and lollium with 0.46 gr of pine plant residue powder respectively, and in the trait of germination rate, the most amount belonged to the control Festuca and the least to the control lollium has been observed. The results of the second experiment demonstrated a significant difference in all measured traits including grass treatment, plant length, fresh and dry weight, germination percentage and rate, and simple effect of plant extract. Moreover, the interaction of grass treatment and plant residue extract showed significant differences in root length, plant length, fresh weight, dry weight, and germination rate and percentage. Comparison of the mean interaction of grass treatment and type of plant residues revealed that the control Festuca had the highest plant length, while Lolium with Silk residue extract had the lowest. The longest and shortest root lengths were observed in control Lolium and Lolium with Silk residues, respectively. The highest and lowest fresh weight were recorded in Lolium control and Lolium grass along with Silk residue extract, respectively. For the dry weight trait, the highest amount was related to the treatment of Lolium grass with pine residue extract, while the lowest dry weight was observed in Festuca grass and Lolium grass with Silk residue extract. In terms of the germination percentage trait, control Festuca and Festuca with pine residue extract had the highest germination percentage, while Lolium with Chinaberry residue extract and Lolium with Silk residue extract had the lowest. The most and least germination rates belonged to Festuca control and Lolium with Chinaberry residue extract. From these discussions, it can be concluded that the allelchemicals in the powder extract of plant residues have had a significant effect on the yield of these two grasses and have reduced growth and germination. Decreased germination due to allelopathic stress can be due to different abnormalities in metabolic activity, seed saturation potential, death of some dividing cells and embryonic abnormalities.
Conclusion
Based on the results of both experiments, it can be concluded that Chinaberry and Silk trees have a stronger allelopathic effect compared to Pine. Additionally, the performance of both grasses in response to residues and extracts containing allelochemicals was slightly different. For instance, in the second experiment, the impact of Chinaberry extract reduced plant length by 28%, root length by 89%, dry weight by 16%, germination percentage by 75%, and germination rate by 85% compared to the Festuca control treatment. Similarly, the effect of Chinaberry extract on Lolium grass resulted in a 96% reduction in plant growth, 97% reduction in root length, 98% reduction in wet weight, 50% reduction in dry weight, 89% reduction in germination percentage, and 89% reduction in germination rate compared to the Lollium control treatment.
Pomology
Mostafa Alizadeh; Mehrab Yadegari; Gholamhossein Davarynejad; Seyyed Hossein Nemati
Abstract
Introduction Pear (Pyrus communis L.) is one of the major fruit trees in the world which grown in temperate zones. In pear breeding program the bigger fruit size with better quality as well as market acceptability are usually in interest. In fruit trees, the size and yield of fruit are closely depended ...
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Introduction Pear (Pyrus communis L.) is one of the major fruit trees in the world which grown in temperate zones. In pear breeding program the bigger fruit size with better quality as well as market acceptability are usually in interest. In fruit trees, the size and yield of fruit are closely depended on cultivar and rootstocks. The variations of nutrient uptake, water use efficiency, photosynthesis performance, and resistance to biotic and abiotic stresses have been reported among different combinations of cultivars and rootstocks. Therefore there is a close relation between cultivar and rootstock with yield and quality of produced fruits. In recent years the semi dwarf clonal rootstocks of pear including OH×F, Pyro and FOX series have received more attention. However the performance of these rootstocks varies depending on scion cultivar, climatic condition, soil type, and planting system. Materials and Methods This experiment was conducted to assay the growth characters and fruit yield of Dargazi and Louise Bonne (Beyrouti) grafted onto PyroDwarf and OH×F69 rootstocks at the Orchard of Astan Quds Razavi, Mashhad, Iran (36°17' N, 59°36' E; altitude. 985 m) during 2018-2019 and 2019-2020. The experiment in both consecutive years was done as a factorial in the base of randomized complete blocks with four replications. The studied cultivars were grafted onto PyroDwarf and OH×F69 rootstocks in late spring 2016. The used water was pit water with 695 µSiemens/cm EC and pH 7.17. Evaluation of plant growth was carried out at the end of the season. The studied traits were included tree height, internode length, vertical and horizontal growth of shoots in the current season, trunk diameter above, below and in the grafting line, leaf area, shoots angel and the number of sucker per tree. These characters were assayed in three trees per each replication. The trunk diameter at the below and above the grafting line was calculated according to trunk circumference that was measured at 10 cm above and below the grafting point. Leaf area in each tree was assayed with area measuring device. Flowering characteristics (start blooming and end of bloom) were determined. Fruit ripening season was also recorded. Yield of trees was assayed by weighting of produced fruits in each tree with a digital scale. The combined analysis of obtain data was conducted by SPSS and differences among means of data were determined by Duncan's multiple ranges test at P≤0.05. The reported data was the mean of two studied years.Results and Discussion The obtained results showed the variation in growth and bearing properties of different combinations of Beyrouti and Dargazi cultivars with PyroDwarf and OH×F69 rootstocks throughout the studied years. The analysis variance showed the significant effect of year, cultivar and rootstock on growth characteristics of pear tree. Also the interaction effects of cultivar and rootstock were significant on tree growth response with exception of internode length. The most studied parameters showed a significant increasing during the two consecutive years. During the studied years the height and width of pear trees were increased up to 10.44 and 14.54%, respectively and the trunk diameter was increased by 10%. According to obtained results the growth of Dargazi cultivar was higher than the Beyrouti cultivar. The effect of OH×F69 rootstock on growth increasing of studied cultivars was prominent than PyroDwarf regards to tree height, shoot length and internode length. However the highest trunk diameter and leaf area density of both cultivars was found in PyroDwarf rootstock. Although the time of blooming and fruit ripening of each cultivar was not influenced by the rootstock but the bearing characters were varied between the Dargazi and Beyrouti cultivars. The highest yield of pear fruit was obtained in Dargazi cultivar and grafting onto PyroDwarf rootstock significantly enhanced the fruit yield in both cultivars.Conclusion The rootstocks have a key role on growth and yield of pear trees as well as their responses to abiotic stress. Rootstocks through influencing the physiological characteristics of grafted scions regulate the quantity and quality of produced fruits. The results of present study showed the variation in growth and bearing properties of different combinations of Beyrouti and Dargazi cultivars with PyroDwarf and OH×F69 rootstocks throughout the studied years. The PyroDwarf rootstock greatly reduced the tree growth and enhanced fruit yield of both Dargazi and Beyrouti cultivars. According to the obtained results the PyroDwarf rootstock is recommended for Dargazi cultivar to reach the highest pear fruit yield under similar environmental condition of present study.
Growing vegetables
Seyyed Ali Yaqoobi; Seyyed Hossein Nemati; Majid Azizi; Mahmood Shoor
Abstract
Introduction Storage potential is an important characteristic for onion. Annually, a large quantity of worldwide onion production deteriorated during storage. The storage ability of onion is influenced by various endo- and exogenous factors including cultivar and storage conditions specially, temperature. ...
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Introduction Storage potential is an important characteristic for onion. Annually, a large quantity of worldwide onion production deteriorated during storage. The storage ability of onion is influenced by various endo- and exogenous factors including cultivar and storage conditions specially, temperature. Based on literature, there is considerable genetic variation for onion storability in onion germplasms from different origins. Rivera Martínez et al. (2005) evaluated the storability of 18 local and 4 commercial onion cultivars in Spain. Their results showed that there was significant variation for storability among different onion cultivars, and interstingly the local cultivars had more storability than commercial ones. Onion storage could also affected by environmental conditions such as temperature. Benkeblia et al. (2000) studied the effects of various temperatures on respiratory parameters of onion. Their finding showed onion respiration were raised by increasing temperature. Likewise, the positive effect of higher temperature on enhancing the growth of sprouts and elevating transpiration has been reported (Miedema, 1998; Yoo et al., 1997). Although, Afghanistan is considered to be one of the origins of onion (Brewster, 1994) there are no reports on estimating the storability of Afghan local cultivars under different temperature conditions. So this research has been conducted to study the effects of various temperatures on storability of native onion cultivars of Afghanistan.Materials and Methods In this research, the plant materials consisted of 10 onion cultivars consisting eight local varieties “Hanaaie Harat”, “Zard-e-Harat”, “Ghermrz-e-Harat”, “Mazar-e-Sharif”, “Kabul”, “Sar-e-Pul”, “Ghonduz” and “Balkhaab” originated from Afghanistan, an Iranian cultivar (“Ghermez-e-Azarshahr”), and a commercial cultivar “Sweet Grano”. The cultivars were stored at two different temperature regimes (5 ºC and ambient temperature), during six-time intervals of 20 days. The average temperature of ambient storage was 13 °C ranging from 8-18 °C. The experiment lasted from November 6th, 2011 to March 5th, 2012. This research was performed in a completely randomized design based on split factorial design, with three replications. For each replication, 50 healthy non-sprouted bulbs were packaged in plastic nets. Storage potential related characteristics such as sprouting percentage, weight loss percentage, sprout length, and sprout weight/bulb weight ratio in 20 days intervals were measured. All data were subjected to analysis of variance (ANOVA) using SAS statistical software. Mean comparisons were calculated at 5% probability level using LSD when the F-value was significant (Fisher’s protected LSD).Results and Discussion The results showed that there were significant differences among cultivars for storage characteristics (weight loss percentage, sprouting percentage, length of sprouts and sprout weight/bulb weight ratio). “Ghonduz” and “Ghermrz-e-Harat” local cultivars had the most and least storage ability, 50% of bulb sprouting after 105 and 35 days, respectively. Rivera Martínez et al. (2005) and Ramin (1999) also reported Spanish and Iranian local cultivars had more storability compared to commercial cultivars. In 5 °C, weight loss of onion bulbs followed a linear model during storage period. But, in ambient storage, it was higher at the beginning and the end of storage period, showing the adverse effects of high temperatures on bulbs weight loss during storage. The interaction between cultivar and time was also significant. It showed onion weight loss differs in various intervals. It could be attributed to the genetic variation and morphological difference such as number and thickness of dry scales of onion bulbs. In ambient storage condition, onion sprouting percentage showed a sharp increase in early storage intervals and became stable in the late intervals. Under 5 °C treatment, it raised as a linear model with very slow slope. It seems that weight loss and sprouting of onion bulbs in ambient storage is dependent on temperature and relative humidity of environment. This finding is in agreement with previous reports (Baninasab and Rahemi, 2006; Forudi, 2005). The sprout length and the ratio of sprout weight to bulb weight was lower in 5 ºC than ambient temperature. Abdalla and Mann (1963) and Yoo et al. (1997) also reported that smaller sprouts were observed in lower temperatures. Conclusion In this experiment, the effects of temperature on storability of some local cultivars of onion originated from Afghanistan were studied in different intervals. It was found that the storage potential of onion bulbs could be influenced by genetic factors and environment conditions such as temperature. Findings of this research showed that sprouting percentage, weight loss percentage and onion sprout growth were less under 5 °C treatment than ambient temperature. Therefore, it was concluded that storing onion bulbs in low temperature conditions can reduce the onion spoilage and improve their storage life. Additionally, cultivars showed highly significant variations for all characteristics related to storage ability. Our findings showed that some local cultivars are more storable than commercial cultivars. Among the cultivars, “Ghonduz” cultivar has more storage potent as compared to all studied cultivars. Our results confirmed that local cultivars can be a good source of desirable genes related to storability. Subsequently, they can exploited to broaden the genetic base of breeding matreials.
Ornamental plants
Toktam Oraee; Mahmood Shoor; Ali Tehranifar; Seyyed Hossein Nemati
Abstract
Introduction: The Hollyhock (Alcea rosea) is a summer flowering biennial plant that is native to China and belongs to the Malvaceae family. It is one of the most valuable ornamental plants, whose 36 species are cultivated in Iran. It is an increasing garden escape, especially in urban areas, and is usually ...
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Introduction: The Hollyhock (Alcea rosea) is a summer flowering biennial plant that is native to China and belongs to the Malvaceae family. It is one of the most valuable ornamental plants, whose 36 species are cultivated in Iran. It is an increasing garden escape, especially in urban areas, and is usually found at foot of walls, in ruderal areas, and in cracks of pavements and old walls. Also, it is sometimes seen on riverbanks (for instance on dikes of the river Maas), dumps or road- and railway banks. Alcea rosea has been used as an herbal plant in folk medicine for treatment of different diseases such as common cold and cough. This plant is antiphlogistic, astringent, demulcent, diuretic and expectorant. Drought is the most significant environmental stress in agriculture worldwide, and improving yield under drought is a major goal of plant breeding. Seed germination and early seedling growth are potentially the most critical stages for water stress. When subjected to drought stress, plant metabolism is interrupted or inhibited by increasing reactive oxygen species (ROS) and lipid peroxidation, resulting in reduced germination, weaker root and shoot growth and even mortality. Plants have evolved oxygen-scavenging systems consisting of non-enzyme antioxidant metabolites, such as proline and various antioxidant enzymes including superoxide dismutase, peroxidase and catalase.Materials and Methods: In order to examine the effect of drought stress on germination indexes, various antioxidant enzyme changes and non-enzyme antioxidant metabolites in Alcea rosea, an experiment was conducted in complete randomized design with three replications. The drought stress treatment was conducted in five levels with osmotic pressures 0, -2, -4, -6, and -8 bar and with using PEG (Poly Ethylene Glycol 6000) on two ecotypes of Alcea rosea (ecotype 1= Mashhad and ecotype 2= Tehran). The seeds are at first sterilized with hypo chloride sodium for two minutes and then washed superficially three times with distilled water. 25 seeds were transferred to a glass petri dish with 10 cm diameter, and for the duration of the experiment, 5 ml solution with different levels was added to each petri dish. After 14 days at 25±1 ºC temperature, the number of geminated seeds in each day was counted and recorded. In the first part of the experiment, after the end of the germination period, the following growth parameters were measured: germination percentage, germination rate, root and shoot length, and the fresh weight of seedling. In the second part, the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), malondialdehyde content, lipid peroxidation in terms of malondialdehyde (MDA) content, and proline index were measured.Results and Discussion: The ecotype, drought treatments and their interaction had significant effects on growth parameters (germination percentage, germination rate, radicle and plumule length, and seedling fresh weight), as well as physiological and biochemical parameters (SOD, POD, CAT, MDA and proline). The highest germination parameters were recorded at Mashhad ecotype. Germination percentage and germination rate were severely affected by drought. Maximum percentage of germination (36) was recorded at control group followed by 32.1 at -2 bar and -4 bar PEG treatments in ecotype 1. Germination percentage in two ecotypes further declined to 33% at -8 bar compared to the control treatment. In both ecotypes, plants had the highest germination rate in control media (zero osmotic potential) but germination rate decreased significantly by decreasing water potential. Germination rate of control seeds of ecotype 1 was 11.7, while that of ecotype 2 was 8. 3. Germination rate in two ecotypes further declined to 56% and 38 %, at -8 bar compared to the control treatment, respectively. Ecotype 2 displayed a significant reduction in radicle and plumule length compared to the ecotype 1. Generally, the radicle and plumule length decreased significantly in relation to the drought stress caused by PEG. Ecotype 2 in -8 bar PEG treatment had the highest (41 and 32%) decrease in radicle and plumule length compared to the control temperature. In two ecotypes (Mashhad and Tehran) of Alcea rosea, increasing PEG concentrations resulted in a decrease in fresh and dry weights. Water absorption is the first germination stage. Due to the probable resistance of the ecotype one, water absorption rate is higher, and as a result, the percentage and rate of germination have increased. Ecotype 1 exhibited higher leaf SOD activities in response to -2 bar compared to -8 bar, but the SOD activities in ecotype 2 showed a general increase trend with increases in the PEG concentration. Drought stress resulted in lower antioxidant enzyme activities (POD) in leaves of both ecotypes compared to that observed at the control treatment, but the activity of CAT increased with the increase of drought stress. In both ecotypes, exposure to -8 bar resulted in significantly higher leaf MDA activities. Plants exhibited higher proline in response to -8 bar treatment compared to the control. By increasing the PEG concentration from control to -8 bar, proline content increased about 90 percent. In this experiment, drought stress reduced the rate and germination percentage and delayed subsequent plant deployment. When plants are subjected to drought stress, their metabolism is interrupted or inhibited by increasing reactive oxygen species (ROS) and lipid peroxidation, resulting in reduced germination, weaker root and shoot growth and even mortality. The recent experiment showed that the activity of two superoxide dismutase and peroxidase enzymes decreased with increasing drought stress, and the activity of the catalase enzyme increased; this is in agreement with the results reported by other studies. On one hand, the increase in the activity of the catalase enzyme indicates that it is perhaps the most important enzyme involved under drought condition, which increased over the course of 14 days. On the other hand, limiting the activity of enzymes and increasing the amount of proline showed that increasing the resistance to drought stress in the plant depends on the accumulation of contaminating substances such as proline.Conclusion: Due to the higher resistance of the ecotype 1 and increase in water absorption, the percentage and rate of germination were increased. If water absorption is disturbed by the seed, the germination activity is slowly applied and the later growth of the roots will decrease the germination rate. Therefore, it seems that Mashhad ecotype with increase of catalase and proline has the highest drought tolerance compared to the other ecotype at germination stage. There was a significant correlation between germination percentage with SOD and POD.
Akram Amiri; Mahmood Shoor; Mina Taghizadeh; Seyyed Hossein Nemati; Ali Tehranifar
Abstract
Introduction: The genus of African violet is the most common genus known among the plants of Gesneriaceae family. This genus has beautiful leaves and flowers that are zygomorph. African violet (Saintpaulia ionantha) is a famous ornamental plant due to its various colors and shapes that an excellent model ...
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Introduction: The genus of African violet is the most common genus known among the plants of Gesneriaceae family. This genus has beautiful leaves and flowers that are zygomorph. African violet (Saintpaulia ionantha) is a famous ornamental plant due to its various colors and shapes that an excellent model system for in vitro regeneration studies because of its tissue culture amenability.Besides the importance of this plant in plant production industry and increasing need for new plant varieties with desired traits, more studies for creating African violet with new features by increasing the ploidy level under the in vitro conditions were not done until now. Polyploidy is a widespread phenomenon in the evolution of flowering plant and a key in plant speciation and diversification. Polyploid plants have been used in plant breeding programs for developing superior varieties and restoring the fertility of interspecific or intergeneric hybrids. Polyploidy in ornamental crops were successfully obtained under in vitro conditions. The chemical colchicine can be used as the most effective substance to obtain polyploid plants. In vitropolyploidization has number of advantages such as treatment of more plants with less material, control of test conditions, lower toxicity of chemicals and high success rate. This study with the aim of polyploid induction was carried out by different concentrations of colchicine at various periods under in vitro conditions.Materials and Methods: The best treatment for the shoot regeneration and proliferation was MS medium with 2 mg/l BA after optimizing the tissue culture process. The study performed as follows: plantlets grown under in vitro in proliferation stage were treated with colchicine. The experiment was carried out in a 2-factorial manner based on a completely randomized design and factors were colchicine concentration at 0.02, 0.05, 0.1% and treatment duration for 18, 24, 48 hours. Evaluated characters of regenerated plants were as follows: plantlets survival percentage (the first month, the second month and the third month), morphological traits (include leaf number, petiole length, leaf length, leaf width, petiole diameter, leaf thickness and variation in form, vein, tip and margins leaf), reproductive traits (flower diameter (mm), number of petal, number of flower in inflorescence, height of inflorescence (mm)), microscopic epidermal cells in samples of diploid and tetraploid and ploidy levels. Assessment of flow cytometery was also used for all of the treated plants with colchicine and some diploid control plants and were expressed in the form of the percentage of diploid, mixoploid and tetraploid plants of African violet.Results and Discussion: Results indicated that tetraploidy induction successfully was changed different morphological and cytological characteristics. Plantlets of treated with 0.02% colchicine in all three times only survived after three months, and with increasing treatment time, plantlets survival percentage was reduced. Thus 0.02% colchicine treatment for 24 hour found effective in inducing in vitro culture polyploidy of African violet. Comparison of the leaves of tetraploid with diploid plants indicated that the leaves of tetraploid plants in length and width were bigger than the diploid ones. The selected tetraploid plants showed a more compact shape than the control plants. Other results revealed that the treated plants with colchicine showed delayed growth. The assessment of microscopic indicated that the epidermal cells of tetraploid were larger than diploid. The results of flow cytometery evaluation showed that with increasing duration of colchicine, the number of diploid plants reduced and were added to mixoploid and tetraploid plants. Most plants were mixoploid.Conclusion: The results of this study showed that the diploid and tetraploid plants indicated significant differences in term of morphological traits like leaf number, petiole length, leaf length, leaf width, petiole diameter, leaf thickness and variation in form, vein, tip and margins leaf, reproductive traits like flower diameter, number of petal, number of flower in inflorescence and also size of epidermal cells and flow cytometery evaluation. These differences can be suitable criterion for separating diploid and polyploid plants from each other. Generally, the combination of tissue culture methods and tetraploidy induction can be used as a rapid strategy for achieving new forms and properties of regenerates in vitro on African violet.
Shahabedin Ahooi; Ladan Ajdanian; Seyyed Hossein Nemati; Hossein Arouiee; Mehdi Babaei
Abstract
Introduction: In the past decades, chemical fertilizers are used by farmers have numerous environmental impacts, including various types of water and soil contamination, and cause many problems to human health and other organisms. Sustainable agricultural policy and sustainable agricultural development ...
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Introduction: In the past decades, chemical fertilizers are used by farmers have numerous environmental impacts, including various types of water and soil contamination, and cause many problems to human health and other organisms. Sustainable agricultural policy and sustainable agricultural development have prompted experts to make greater use of soil organisms to meet the plant's nutritional needs, and that is why the production of biofertilizers began. Biological imbalances in the field of sustainable agriculture can be attributed to the fungi of myoderma and its species. According to various studies, it seems that this microorganism having high ability to compete for food and space, and the establishment of spores in the environment and in particular soil around the roots of most crops and non-farm and can induce plant resistance not only reduces pathogen agents Vermicompost is a microbiologically rich, nutrient-rich, organic modifier that is produced by the interaction between earthworms and microorganisms during the decomposition of organic matter. This type of organic fertilizer contains the waste of certain species of earthworms as a result of alteration, conversion, and relative organic residues as they pass through the digestive tract of these animals. Research has shown that vermicompost has a positive effect on growth, crop development, and crop yield. The aim of this study was to investigate the effect of different amounts of vermicompost and different concentrations of Trichoderma Bi isolate on different properties of celery. Materials and Methods: This study was conducted to investigate the effects of Trichoderma and vermicompost as a biofertilizer promoting growth in research greenhouse at the Ferdowsi University of Mashhad with an average daily temperature of 15-27 °C and relative humidity of 40-70%. The present study was a factorial experiment based on a completely randomized design with three replicates as a pot experiment under no-tillage conditions in the greenhouse. The first factor consisted of four concentrations of Trichoderma harizianum isolate Bi: zero percent (control), 5%, 10%, and 15% volume of 50 liters of water consumed. The second factor also included four vermicompost treatments: zero percent (control), 25 percent, 50 percent, and 75 percent pot volume. Celery seeds were sown in transplant trays. The transplants were ready for transfer to the main litter after 10 weeks. The pots used were of plastic-type with a span diameter of 20 cm and a height of 25 cm. The plant media consisted of a mixture of 20% cocoon and 80% perlite, the roots of which were easily separable. After full harvest of plants at the commercial size, when a complete set of petiole sets was created (40 days after transplanting) morphological traits including the fresh and dry weight of roots and stems, a number of leaves, stem diameter, stem and root lengths were evaluated. The dry and dry weight of plant root was measured using a digital marking scale and with an accuracy of 0.01 g. Dry weight was determined after placing the specimens in the oven at 72 ° C for 48 hours. Also, the stem diameter was measured using a caliper machine with 0.01 mm accuracy. Root and shoot lengths were measured separately in the laboratory by a ruler in cm. Chlorophyll a, b and carotenoids were read at 663 nm, 653 nm and 470 nm for absorption by spectrophotometer, respectively. Data were analyzed using JMP8 software and ANOVA was performed using the LSD test at 5% probability level. Charts were drawn using Excel 2013 software. Results and Discussion: The results of this study showed a positive and optimal effect of combined vermicompost and Trichoderma fungi. The highest shoot dry weight (49.23 g), leaf number (46), stem diameter (15 mm) and chlorophyll and carotenoid were observed in the effect of vermicompost and Trichoderma fungi compared to the control treatment. The main stem length (77.20 cm) was affected by the fungus with a 10% concentration at the highest rate compared to other treatments. Also, 50% vermicompost treatment had the highest root length (36.66 cm). The highest chlorophyll a was observed at 75% vermicompost interaction at 15% fungi concentration (10.02 mg / g fresh leaf weight). Application of vermicompost in the culture medium and application of Trichoderma fungus extract resulted in improved growth and yield. As can be seen in the results, the best treatment was 15% and 75% vermicompost, respectively. They can be used to improve plant growth and function. Many researchers believe that mainly isolates of Trichoderma produce biochemical stimuli to stimulate plant growth or reduce the inhibitory effects of certain compounds, biological and chemical toxins. According to available reports, the application of vermicompost with 30% volume in ornamental Lilium increased leaf area, fresh and dry weight of plant and plant height. So, fresh and dry weights of bean stem under vermicompost application significantly increased compared to the control treatment, which is in line with the results of this study. However, the researchers believe that the wet and dry weight gain of the plant body if used with vermicompost is probably due to the high amount of humic acids in this biofertilizer. Conclusion: Based on the results of this study and other studies on vermicompost as an enriched fertilizer with numerous growth enzymes and hormones, as well as Trichoderma, it can be concluded that the use of bio-fertilizers plays an important role in the production of high-quality products. Also, the effect we find depends on the concentration used, but in the end, even their application at the lowest concentration compared to the control treatment increased growth and morphological characteristics of the plant. Overall, the results of this study showed that, in the case of the studied species, the combination of vermicompost and Trichoderma had a significant advantage over their separate application, and considering the superiority results of most traits at the time of application of these two together. Therefore, a combination of Trichoderma and vermicompost based on the results (concurrent use of 15% fungus extract and 75% vermicompost) is recommended to improve plant growth and yield.
Mina Ghazaeian; Gholamhossein Davarynejad; Kamal Ghasemi Bezdi; Seyyed Hossein Nemati
Abstract
Introduction: The walnut family (Juglandaceae) consists of approximately 60 species of deciduous trees is native of the American continents, Europe, and Asia. Pecan (Carya illinoensis) is belonged to the Juglandaceae family and is one of the most valuable nut products all over the world. Embryo culture ...
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Introduction: The walnut family (Juglandaceae) consists of approximately 60 species of deciduous trees is native of the American continents, Europe, and Asia. Pecan (Carya illinoensis) is belonged to the Juglandaceae family and is one of the most valuable nut products all over the world. Embryo culture techniques for plant breeding as well as basic studies in physiology and biochemistry are widely used. The low percentage of germination and the long propagation cycle and the need for stratification treatments from three to six months are the most important barriers to the development of high yielding cultivars through hybridization. Plant regeneration methods from embryo culture in vitro allows overcoming the barriers of hybridization, as well as obtaining higher and faster multiplication rate of plants of an elite genotype.
Materials and Methods: In this experiment, an adapted native genotype of pecan in Gorgan city, Golestan province, Iran was selected. The mature fruits were harvested after five months of pollination. They were immediately transferred to the laboratory. For cold pretreatment, nuts packed in a paper bag and stored in 4-5ºC for 15 days. The effect of two types of culture medium, growth regulators and seed pretreatment (15 days at 4-5 °C) on germination of mature embryos of pecan has been determined. Murashige and Skoog (MS) and Woody Plant Medium (WPM) and IBA (0 and 1 mgl-1), BAP (0, 1 and 2 mgl-1) and GA3 (0 and 1 mgl-1) media were used to embryo rescue evaluation. The data obtained were statistically analyzed in completely randomized block design (RCBD). Each treatment was replicated at least third, and each replicate consisted of two zygotic embryos. Means of germination period, percent of seed germination, root and shoot length and leaf number in different media and various PGRs combination were compared based on LSD at p ≤0.05.
Results and Discussion: The results showed, although cold pretreatment for 15 days had no effect on germination period, root length and number of leave but also, effect on germination percentage and shoot length. There are some different hypothesis about the effect of cold pretreatment on embryo germination between researchers. Some researchers believed that, there is low efficiency in embryo germination in lack of cold pretreatment and GA3. Cold pretreatment or GA3 reduce the ABA level and promote embryos germination. The others reported poor germination for somatic embryos when they treated with GA3 and cold pretreatments. Pearce et al. (1987) reported that GA3 and substrate of GA3 can be increased during the chilling process as ABA levels decrease. Furthermore, application of exogenous GA3 induces germination. Tang et al. (2000) reported that somatic embryos germination poorly happened in cold condition and addition of GA3 did not change the poor germination. Kaur et al. (2006) and Peyghamzadeh and Kazemitabar (2010), reported that the embryo germination in Juglans regia L. was higher when GA3 and cold pretreatments were simultaneously applied as compared to those when applied separately. In this experiment, media has no effect on embryo germination period but, could effect on other parameters. As the results showed, MS media showed the maximum percentage of germination, root and shoot length and number of leave in both condition (with and without cold pretreatment). In this experiment root length of germinated pecan embryo was higher in MS medium. Mapelli et al. (2001) reported that seed germination resulted in marked changes in the metabolism of free amino acids in walnut cotyledons. About 52% of the total free amino acids in one-month-old seedlings was present in the cotyledons and about 26% was in the taproot. The concentration of free amino acids in the taproot was similar to that in the embryonic axis, and greater than that in the cotyledons. T11 (1mg/l-1 IBA, 1mg/l-1BAP and 1mg/l-1 GA3) and T12 (1mg/l-1 IBA, 2mg/l-1BAP and 1mg/l-1 GA3) treatments were the highest in germination percentages in both treatment (with and without cold pretreatment). There was no significant differences between 1 mgl-1 and 2 mgl-1 of BAP.
Conclusion: Pecan as like walnut, is considered to be one of the most recalcitrant species in vitro. It is necessary to determine the optimal culture conditions to establish it for shortening time in seed propagation. This seedling could be applied as primary material for breeding programs, grafting and physiology study. The best growth of micro plant achieved in MS medium with 1 mgl-1IBA, 1 mgl-1GA3 and 2 mgl-1BAP.
Maedeh Aghdaei; Seyyed Hossein Nemati; Leila Samiei; Ahmad Sharifi
Abstract
Introduction: Pepino (Solanum muricatum Aiton) is a diploid herbaceous plant belongs to the Solanaceae family, which is growing in subtropical zone, originates from Andes in South America. It is commercially grown for its fruit, which is appreciated not only for food but also for its appearance, in South ...
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Introduction: Pepino (Solanum muricatum Aiton) is a diploid herbaceous plant belongs to the Solanaceae family, which is growing in subtropical zone, originates from Andes in South America. It is commercially grown for its fruit, which is appreciated not only for food but also for its appearance, in South American countries, including Bolivia, Colombia, Ecuador and Peru, as well as in countries such as New Zealand and Australia. Pepino is propagated by seed, cutting, and tissue culture methods. Most pepino cultivars are sexually fertile and produce viable seeds, but their seeds have poor germination and high level of heterozygosis causing to highly variable plants. Both mentioned negative aspects have limited the mass production of this plant through seed. In this case, stem cutting is used as the most common way of propagating pepino led to transmission of viral diseases and increasing propagation costs as two main limiting factors of pepino propagation. So, micropropagation systems are a promising tool to produce disease-free clonal plant material with low costs. Therefore, the present study was aimed to assess the effect of different media and plant growth regulators on micropropagation traits of pepino.
Materials and Methods: Three separate experiments were carried out in institute of plant sciences of Ferdowsi University of Mashhad in 2016. Pepino seeds were bought from company of Plant World Seed, UK, were cultivated on MS medium. Grown plants were used as source of providing explants. Four mediums, including MS, ½ MS, SH and B5 were used to determine the best culture medium for shoot regeneration of pepino using single node explant. A factorial experiment was conducted based on a completely randomized design. Some growth properties such as number of shoots, shoot length, number of roots, root length, leaf number and leaf length were evaluated after two and four weeks. In proliferation experiment, MS medium was compared with MS supplemented with different concentrations of BA (0.5, 1 and 2 mg L-1) and Kin (0.5, 1 and 2 mg L-1) applied as combined treatments, and also BA used alone at concentrations of 2, 4 and 6 mg L-1 that was conducted based on a completely randomized design. For rooting of explants, an experiment was conducted based on a completely randomized design containing of two concentrations of IBA (at 0.3 and 0.6 mg L-1) and three concentrations of NAA (at 0.3, 0.6 and 0.9 mg L-1) in MS medium. Some growth properties including root number and length, root density and root quality were evaluated after four weeks
Results and Discussion: Results indicated that micropropagation rate of pepino was affected by culture medium type. The highest shoot length, number of root, root length and leaf number were obtained in MS medium, although statistically there was no significant difference between MS and ½ MS media. The highest number of shoots and leaf length were observed in MS medium, which led to a significant difference with other media (½ MS, SH and B5). Overall, Based on obtained results MS medium was the best culture medium for micropropagation of pepino using single node. In the proliferation experiment, the highest shoot and leaf number and plant color were obtained with using 2 mg L-1 BA + 1 mg L-1 Kin, whereas the highest shoot length and leaf length were observed in the 1 mg L-1 BA + 2 mg L-1 Kin and 1 mg L-1 BA+1 mg L-1 Kin treatments, respectively. Increasing in concentration of BA up to 2 mg L-1 in combination with Kin had a positive effect on shoot proliferation, while applying BA at concentration 2, 4 and 6 mg L-1 alone led to decrease in proliferation. Results obtained from rooting experiment showed that the highest root number, root density and root quality were obtained using IBA at the concentration of 0.6 mg L-1, whereas the highest root length was observed by applying IBA at concentration of 0.3 mg L-1, which led to a significant difference with other treatments. Furthermore, results indicated that the effect of IBA on rooting of pepino microshoots was more than NAA.
Conclusion: Generally, the best results were obtained by MS medium, 2 mg L-1 BA with 1 mg L-1 Kin for shoot proliferation, and IBA at concentration of 0.6 mg L-1 for the rooting of pepino nodal segments.
Hassan Farhadi; Majid Azizi; Seyyed Hossein Nemati
Abstract
Introduction: Drought is an event that happens due to lack of rainfall in a period of time. The occurrence of drought, reduces available soil water, but water losses through evaporation and transpiration is constantly increasing. The first stage of the plant, which may be faced with drought, is ...
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Introduction: Drought is an event that happens due to lack of rainfall in a period of time. The occurrence of drought, reduces available soil water, but water losses through evaporation and transpiration is constantly increasing. The first stage of the plant, which may be faced with drought, is germinating. Since Germination begins with water uptake, lack of water at this stage in terms of duration and intensity of the stress causes or reduce germination percentage and germination rate. Because of the heterogeneity of soils in the field and lack of control of the environmental factors such as drought, laboratory research on the stress is considered. Some of these methods can be used to study the reaction of the landraces to the solutions from materials polyethylene glycol. This study aimed to investigate the characteristics of the native population of fenugreek germinating rate under drought stress conditions and identify landrace was incurred.
Materials and Methods: To investigate the effect of drought stress on germination and seedling growth characteristics of native landrace fenugreek, an experiment was conducted in a Completely Randomized Design with three replications in Seed Laboratory of University of Mashhad in 2014. The experiment treatments consisted of eight landraces of fenugreek of Esfahan, Tabriz, Hamedan, Sari, Challous, Amol, Mashhad and Yassooj on polyethylene glycol 6000 (PEG 6000) at four levels: (0, 3, 6 and 9 times) were replicated three times. The stress test for the simulation of polyethylene glycol 6000 was used and the amount of material needed to create each of the stress levels using the formula Michel and Kaufmann respectively. To (control) and distilled water was used. Landraces seed of fenugreek the city of Esfahan, Tabriz, Hamedan, Sari, Challous, Amol, Mashhad and Yassooj purchased before the start of the experiment with a three percent solution of hypochlorite for two minutes to disinfect and then were washed three times with distilled water on the twelfth day, seeds and traits such as root length and root out the Petri measure.
Results and Discussion: In the study the interactions between landrace and drought, the rate of decline in most of the traits was related to native populations of fenugreek, Tabriz, Hamadan, Sari, Challous, Amol, Yasuj and Mashhad, respectively (-9 times) compared to control (0 times). As can be seen, the Isfahan landrace was more stable in most of the traits due to changes in drought levels. On the other hand, as the drought stress increased, the percentage and rate of germination of fenugreek seeds decreased. Fenugreek seed germination rate and percentage with increased drought stress the germination rate under drought stress sensitivity was higher than the percentage of germinating. The plants grow in different stages react differently to drought show seed germination and seedling production and a dry condition indicates that the plant is a potential for drought tolerance but that does not mean that the seedlings start to grow in dry conditions, can continue to grow in the same conditions.
Azadeh Rashidi; Seyyed Hossein Nemati; Narges Bozorg
Abstract
Introduction: Transplant production is one of the most important commercial production of melon. Transplanting of seedlings with strong and healthy stems and roots will be successful. Environmental conditions, such as light, affect the proper growth of healthy transplants. The light provides the necessary ...
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Introduction: Transplant production is one of the most important commercial production of melon. Transplanting of seedlings with strong and healthy stems and roots will be successful. Environmental conditions, such as light, affect the proper growth of healthy transplants. The light provides the necessary energy for photosynthesis. Due to the stimulation of the activity of photosynthetic pigments and light receptor pigments, it can be expected that plant performance increase by improving the quality and quantity of light. High pressure sodium and fluorescent lamps are common artificial light sources in greenhouses but because of their high power consumption, heat generation and the light spectrum that the plant does not use, application of LED is taken into consideration. The production of specific spectrum of light and the possibility of spectral composition are advantages of LED lamps. The aim of this experiment was to investigate the effect of light quality and cultivar on some physiological and vegetative characteristics of two melon cultivar seedlings, Ghasri and Khatooni, which are among the most important melon cultivars in Iran.
Materials and Methods: To investigate the effect of light quality and cultivar on vegetative characteristics of melon (Cucumis melo Gr. Inodorus) transplants, a research was conducted from April 4 to May 10, 2016 as split plot experiment in completely randomized design with five replications and the seedlings of Khatoonia and Ghasri cultivars were treated under different light quality include two combinations of blue and red spectrum with ratios of 15%B : 85%R , 30%B : 70%R, fluorescent lamp and HPS lamp. In order to set spectra combinations, LED lamps of Red (R625nm ) and Blue (B476nm) were used. The 85%R: 15%B ratio was obtained through using of 340 R lamps plus 60 B lamps and the 70%R: 30%B ratio was obtained by the usage of 280 R lamps plus 120 B lamps on separate Plexiglass plate. Closed growth chambers without natural light were used. The size of LED growth chambers were 70×60×60 cm3 and the size of HPS lamp growth chamber was 120×60×60 cm3. The seeds were planted at a depth of 4 cm and were transplanted to growth chamber equipped with the desired light compounds. Light intensity was 65 mmol and duration of light was 16 hours. Data was collected when transplant had four leaves. Emergence speed index, mean time for emergence of transplants, fresh and dry weights of foliage and root, root volume, leaf area and thickness, leaf number, height, height to diameter ratio, stem caliper, chlorophyll a, chlorophyll b, chlorophyll total and carotenoids contents were measured.
Results and Discussion: The result showed that the interaction effect of light quality and cultivar was significant on fresh and dry weights of foliage and root, root volume, leaf area and thickness, height to diameter ratio, chlorophyll a, chlorophyll b, chlorophyll total and carotenoids contents. The fresh and dry weights of foliage of Ghasri cultivar and fresh weight of root of Khatooni cultivar under 15%B: 85%R ratio, the dry weight and root volume of Ghasri cultivar under, 30%B: 70%R ratio, the chlorophyll a and carotenoids contents of Ghasri cultivar under, 30%B: 70%R ratio, the chlorophyll b and chlorophyll total contents of Khatooni cultivar under, 30%B: 70%R ratio were superior. The results of this study showed that the use of compounds of blue and red lights increased the dry matter and development of roots in studied plants. Proper dry matter and root development are important because they make the plant resistant to environmental stress. However, the effect of light quality was affected by the cultivar. For example, Ghasri cultivar showed the highest fresh and dry weights of foliage under 15%B: 85%R ratio and with the increase of blue light level, these two traits decrease significantly, but this results was not obtained in Khatooni cultivar. The results showed that the light quality affected leaf area and thickness of two cultivars in a different way. In Ghasri cultivar the highest leaf area and thickness were obtained under, 30%B: 70%R ratio. In Khatooni cultivar, under, 30%B: 70%R ratio, the highest leaf area and under fluorescent light, the highest leaf thickness were observed. The effect of blue light on the variation of leaf area among plants has been reported differently. The leaf area plays an important role in photosynthesis in plants and with its increase, photosynthesis and plant growth improved. The result showed that the interaction effect of light quality and cultivar was not significant on emergence speed index, mean time for emergence of transplants, leaf number, stem caliper and height. The highest emergence speed index and mean time for emergence of transplants were obtained under, 30%B: 70%R ratio without significant difference with 15%blue: 85%red ratio. Leaf number was lowest under HPS lamp and there is no significant difference in leaf number among 15%B: 85%R ratio, 30%B: 70%R ratio and fluorescent lamp. The highest stem caliper and lowest height were obtained under, 30%B: 70%R ratio. Interaction of phytochromes and cryptochromes due to different levels of blue and red lights lead to the formation of different concentrations of gibberellins and this affects the height of the plants. In some plants, increasing the amount of blue light leads to a decrease in the secretion of this hormone and as a result, plant heights are reduced. The results showed that the blue light had a positive effect on the increase of stem caliper and increasing transplant diameter has a positive effect on its establishment and development after their transfer to the main planting site.
Conclusions: The result showed that the application of the blue and red spectra compared to fluorescent and HPS lamps improved the quality of transplants growth. Improve or mitigate results and the performance in traits such as fresh and dry weights of foliage and root, root volume, leaf area and thickness, height to diameter ratio, chlorophyll a, chlorophyll b, chlorophyll total and carotenoids contents depend on light quality and cultivar.
Maryam Kamali; Mahmood Shoor; Seyyed Hossein Nemati; Amir Lakzian; Hamidreza Khazaie
Abstract
Introduction: Water deficiency is one of important abiotic stresses that severely effects on plant growth. The effects of drought range from morphological to molecular levels and are evident at all phenological stages of plant growth at whatever stage the water deficit takes place. Growth is accomplished ...
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Introduction: Water deficiency is one of important abiotic stresses that severely effects on plant growth. The effects of drought range from morphological to molecular levels and are evident at all phenological stages of plant growth at whatever stage the water deficit takes place. Growth is accomplished through cell division, cell enlargement and differentiation, and involves genetic, physiological, ecological and morphological events and their complex interactions. The quality and quantity of plant growth depend on these events, which are affected by water deficit. Cell growth is one of the most drought-sensitive physiological processes due to the reduction in turgor pressure. Under severe water deficiency, cell elongation of higher plants can be inhibited by interruption of water flow from the xylem to the surrounding elongating cells. Impaired mitosis, cell elongation and expansion result in reduced plant height, leaf area and crop growth under drought. Chlorophyll content is one of the major factors affecting photosynthetic capacity changing in chlorophyll content of plant under drought stress has been observed in different plant species and its intensity depends on stress rate and duration. Chlorophyll content of leaf is indicator of photosynthetic capability of plant tissues. In the mid-80s, RWC was introduced as a best criterion for plant water status which, afterwards was used instead of plant water potential as RWC referring to its relation with cell volume, accurately can indicate the balance between absorbed water by plant and consumed through transpiration.
Materials and Methods: To study the effects of drought stress on three varieties of petunia, a factorial experiment based on randomized complete block design with four replications was conducted. The treatments consisted of four irrigation levels ((100% control), 80%, 60% and 40% of field capacity) and three varieties of petunia (Supercascade, Tango blue and Tango white). After planting and transplanting and after full deployment in the pot, water stress treatments were applied on three varieties of petunias. At the end of each week fully blossomed flowers were counted, flower diameter, peduncle length and corolla length were measured. In order to determine the stability of the cell membrane electrolyte leakage index was measured. Specific leaf area (SLA) was determined. The amount of chlorophyll a, b, total and carotenoid and relative water content in the leaves were measured. Statistical analysis was performed using the software MSTAT-C. EXCEL was used for diagramming software. Means were compared using LSD test with a 0.05 significance level.
Results and Discussion: Results indicated that interaction impacts of variety and irrigation on dry weight, leaf area, flower number, flower diameter, length Corolla, chlorophyll content, electrolyte leakage, relative water content and proline content was significant. The most shoot dry weight (76/1 g) was in control stress (100% FC) and Tango White variety. Also the most leaf dry weight (07/2 g) and root dry weight (g 43/0) were in Tango Blue variety. With increasing drought stress from 100% FC to 40% FC, leaf area decreased in Supercascade from 314 to 49, in Tango Blue from 405 to 44 and in Tango White from 459 to 69 cm2. In 80% FC, electrolyte leakage increased in all varieties (Supercascade variety 2%, Tango Blue 10% and in Tango White 3%) compared to control. Also electrolyte leakage increased in Supercascade 17%, in Tango Blue 9% and in Tango White10% in 40% FC compared to control. Comparison of interaction effects of drought stress and variety also showed the most proline had accumulated in Tango White and drought 40% of field capacity and then in Tango Blue and stress 40% of field capacity. Generally two varieties of Tango Blue and Tango White in control irrigation had better growth and also in low irrigation were more resistant.
Mina Nurzadeh Namaghi; Gholamhossein Davarynejad; Hossein Ansari; Seyyed Hossein Nemati; Ahmad Zarea Feyzabady
Abstract
Introduction: Mulching is a useful practice with the potential of conserving moisture, reducing evaporation, modifying soil temperature, and improving aeration as well as releasing nutrients in the soil profile. Mulching involves the use of organic materials (e.g. crop residues, straw, grasses, and farmyard ...
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Introduction: Mulching is a useful practice with the potential of conserving moisture, reducing evaporation, modifying soil temperature, and improving aeration as well as releasing nutrients in the soil profile. Mulching involves the use of organic materials (e.g. crop residues, straw, grasses, and farmyard manure) or inorganic and synthetic materials (e.g. polyethylene sheets, and gravels). Application of mulch can notably as a soil management method influence agricultural crop production despite the limited amount of water available in arid and semi-arid areas.
Materials and Methods: Field studies were conducted in a randomized complete block design with five replications for two years in 2014 and 2015 at Feyzabad city, Iran (34° 40´ N, 58° 25´ E). The aim of the study was to investigate the effects of different mulching treatments on physiological traits of pistachio (Pistaciavera L.) in relation to soil temperature and moisture variations. The treatments were: M1 (whitepolyethylene film mulch covered with two centimeter of soil with width cut), M2 (white polyethylene film mulch covered with two centimeter of soil with circular cut), M3 (green polyethylene film mulch which was as a single layer pulled on water strip so that irrigation water passes under the coverage), M4 (woodchip mulch), M5 (barley residue mulch), and CK (control or no mulching). The measurement (soil moisture and temperature, stomatal conductance and leaf temperature) was carried out periodically on the central trees (4 trees per treatment) every 12, 24 and 36 days after irrigation between 12-15 pm from early-May to late September. The data obtained from the experiment were subjected to an analysis of variance (ANOVA) by using SAS 9.1 software. Difference between means was compared using least significant difference test (LSD) at 5% level (p ≤ 0.05).
Results and Discussion: The results showed that the mean soil moisture percentage in all mulches especially plastic mulches was higher compared to the control over two years of experiment. During the two years of experiment, treatments of M1, M2 and M3 with 35.2, 35 and 38.9 °C and treatments of M4, M5 and CK with 28.8, 29.6 and 32.8 °C indicated the highest and lowest average soil temperature at 20-30 cm soil depth, respectively. Also, similar results were observed at 50-60 cm soil layer. M3 and CK treatments with 47.7 and 41.1 mmol/m-2s-1 had the highest and lowest mean stomatal conductance, respectively. The mean values of leaf temperature during the growing period under M1, M2, M3, M4 and M5 treatments were 2.2, 1.9, 2.4, 1.7 and 1 °C in 2014, and 1.8, 2.2, 2.2, 1.5 and 1.1 °C in 2015 lower than CK (control) treatment, respectively. According to correlation results significant difference was not observed between stomatal conductance and soil moisture, but the relationship of this parameter with soil temperature and relationship between leaf temperature with soil moisture and soil temperature were significant. Thus, this results demonstrate that stomatal conductance and soil temperature weresignificantly affected by soil temperature, so that an increase in soil temperature to 40 °C, canbe improved these traits if other soil and climatic factors werenot limiting. The results also showed that M3 mulch (plastic mulch on soil surface) despite the higher soil water storage due to providing of soil high temperatures, can be leads to moisture stress in heavy cropping year.
Conclusions: The results of thisstudy showed that organic and inorganic mulches improved the soil moisture content throughout the two growing seasons, thus reduced negative effects of water shortage. Mulch treatments indicated smaller fluctuations in soil temperature compared to CK (control) treatment, possibly due to insulating against heat radiation. The results of our study suggested that increase in soil temperature in M3 treatment, despite higher water storage, had limited impacts on improving stomatal conductance and leaf temperature from early July in the second year. Thus, it seems that high soil temperature in this treatment in ON year that trees' water and nutrients requirement is higher than OFF year acts as a limiting factor and restricts absorption of water and nutrients, resulting in the decrease of physiological activity of trees. Generally, it is conclude that white polyethylene films covered with soil especially M2 and organic mulches due to providing optimum soil temperature, preserving soil moisture and lower cost can be better management options to be applied in pistachio orchards especially in arid and semiarid areas, where traditional irrigation methods with high evaporation level are used.
Fariborz Alizadeh Zarmehri; Gholamhossein Davarynejad; Reza Khorasani; Seyyed Hossein Nemati; Peyman Keshavarz
Abstract
Introduction: Pear producers around the world are looking for faster return of capital and saving in labor costs, achieved well by high density orchard planting. Choosing a good combination of scion and rootstock is critical for production of fruit trees because the relationship between the rootstock ...
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Introduction: Pear producers around the world are looking for faster return of capital and saving in labor costs, achieved well by high density orchard planting. Choosing a good combination of scion and rootstock is critical for production of fruit trees because the relationship between the rootstock and scion has a decisive effect on plant water relations, leaf gas exchange, plant size, flowering, fruit production, fruit quality and production efficiency. Nowadays in some regions, growers are using quince rootstock for pear orchard establishment. Using the quince rootstock alleviates many problems in pears orchard, but graft incompatibility between pear scion and the quince rootstock, and resistance to frost and alkaline soils are some problems restricting the use of this rootstock. In most pear-producing regions in Iran, pear seedling is used for pear propagation. Due to the problems of pear including overgrowth and late precocity (after 4 -5 years), this research evaluated the effects of pear clonal rootstock (Pyrodwarf, OHF and Quince c) on some growth characteristics in comparison to pear seedling (Pyrus communis).
Materials and Methods: This research was conducted during 3 years from 2014-2016 in Chenaran (36.6, 59.1) in northeast of Mashhad. Maximum and minimum temperatures were 40 and -22 ˚C, respectively, elevation 1176m and the average annual rainfall 240mm. The same tissue culture rootstocks from two cultivar Pyrodwarf and OHF with Quince rootstock and annual seedling from Dragazi pear were selected in August 2013 and T-budded with three commercial cultivars Spadona, Ntanz and Sebri. In the spring of 2014, after relieving frost danger, the trees were planted in field with row space 4×2m. In order to investigate dwarfing effect of rootstock on scion cultivar, some important vegetative factors that represent dwarfing effect of rootstock including trunk cross sectional area, height of tree, amount of lateral branch growth, tree growth rate during growth season, mean of growing buds on each lateral branch, and stem and leaf water potential were measured. This test was conducted in factorial based on randomized complete block design with 4 replications. Each plot was included one hybrid combination. MSTATC and Excel software were used for data analysis, and differences among means were compared by using LSD test.
Results and Discussion: Different rootstocks did not show any significant difference in terms of leaf water potential. Leaf water potential during the seasons had a constant time course about scion cultivar on all rootstock so while temperatures rise throughout the season it reduced the amount of leaf water potential. The effect of cultivar was significant on leaf water potential so that the highest water potential was related to Natanz and the least water potential was related to Serbi cultivar. The effect of rootstock and scion both on stem water potential was significant. Therefore the highest and lowest stem water potential was recorded for cultivars grafted onto the seedling and quince rootstock, respectively. In this study, a significant relationship was observed between minimum stem water potential (mid-day) and branch growth rate during the growing season. With reducing stem water potential as a result of temperature increase, the amount of branch growth reduced. The effect of both scion and rootstock on chlorophyll index was significant. In this investigation, all 4 rootstocks had different effects on chlorophyll index. The highest chlorophyll index was related to cultivar Natanz and the least was related to Spadona. Sebrie had medium chlorophyll index. Rootstock effect on vegetative growth of the scion was not significant but the effect of scion was significant at 5% level. Pear seedlings with vigorous growth had more long branches than other rootstocks. In the present research, seedling rootstock also induced higher growth of lateral branches. Furthermore, quince rootstock induced the least growth of lateral branch during 3 years of the investigation. In addition, quince rootstock had the minimum tree height and pear seedling had the maximum tree height. Internode length in dwarfing rootstock was less than seedling. Both scion and rootstock had significant effect on trunk sectional area so that at the end of three years, cultivars grafted on seedling rootstock had the highest trunk cross sectional area, and cultivars on quince rootstock had the lowest TCSA. Two vegetative pear rootstocks (Pyrodwarf and OHF) did not show significant difference from each other. Pyrodwarf and OHF rootstock showed good compatibility with Natanz and Spadona scions like seedling rootstock. On the other hand, the survival percentage on quince rootstock was really low.
Conclusion: This investigation showed that pear rootstock had less vegetative growth than seedling rootstock and induced dwarfing effect on scion growth during 3 years but quince rootstock had more dwarfing effect.
Ali Farhadi; Hossein Arouiee; Seyyed Hossein Nemati; Reza Salehi; Francesco Giuffrida
Abstract
Introduction: Salinity stress is regarded as one of the most important abiotic factors in plant limiting growth, particularly in arid and semi-arid regions. The reduction of plant growth by salinity stress has been well documented. When water supply is limited, plant structure is modified by increasing ...
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Introduction: Salinity stress is regarded as one of the most important abiotic factors in plant limiting growth, particularly in arid and semi-arid regions. The reduction of plant growth by salinity stress has been well documented. When water supply is limited, plant structure is modified by increasing the root: shoot ratio. To reduce of losses in vegetative growth and production of plant and to improve water use efficiency under saline conditions in high-yielding genotypes grafting them onto rootstocks could bereduced the effect of saline stress on plant shoot. Grafting is a routine technique in continuous cropping systems. Most of the species of cucurbits are distributed in the dry regions. The objective of this studywas investigated the effectiveness of salinity stress on accessions of cucurbita and hybrid inter specific which enter from another country to Iran.
Materials and Methods: This research was conducted in laboratory and greenhouse at the Research Center of Agricultural and Natural Resources of Isfahan during 2013-2014 growing season. A factorial experiment based on completely randomized design with three replications was conducted for rootstock and irrigation water salinity.. In the first experiment 25 seeds of rootstocks were sown in petri dishes with 10 cm diameter and irrigated by 10 ml of saline water. Rootstocks included 20 different local landraces and interspecific hybrids (C.moschata cv. Isfahan and Koshk, C.pepo cv. Alvar, Tiran, Koshk and Asgharabad, C. maxima cv. Kermanshah, Shahreza, Mohamadiyeh and Alvar, Lagenaria Siceraria, Luffa cylindrica, Trichosanthes cucumerina, RZ-Ferro, Es113, Ews910, Ews909, Ews913, 426 and Es152). Salinity stress was 6 levels (0, 2, 4, 6, 8 and 10 ds/m of NaCl). Germination, diameter of stem, height of root and stem, shoot and root fresh mass, vigor index and root: shoot ratio were evaluated. In the second experiment seeds were sown in plastic pot by soil media. Seedlings were irrigated daily with 200 ml of saline water (0, 2 and 4 ds/m) for 35 days. Excess solution was allowed to drain from the plants through drainage holes in the base of the pots. Hybrids of 152,426 and Trichosanthes cucumerina, Luffa cylindrical and Cucurbita pepo con. Pepo var. Styriaca was added. In the second phase measured shoot and root dry mass, SPAD index, relatively water content and seedling vigor index.
Results and Discussion: the results showed that all parameters were significantly influenced by salinity except root: shoot ratio in Petri dish. Salinity stress reduced chlorophyll index (SPAD), relative water content in cucurbita leaves than control (without saline) and also seeds germination, seedlings fresh mass and seedlings vigour by 51%, 53%, and 75 % respectively. .The shoot dry biomass of local landrace and inter specific hybrids in both experiment decreased linearly in response to increasing saline stress. Recorded data in Petri dish and plastic pot were significantly influenced by rootstock, whereas no significant difference was observed on germination rate and vigor index for plastic pot in greenhouse. The lowest seed germination percentage, seedling growth, vigor index and root: shoot ratio recorded on high saline concentration. NaCl threshold damage to cucurbits was evaluated 4 ds/m. Populations of C. maxima cv.Kermanshah and Shahreza, C.moschata cv. Isfahan and inters pecific hybrids Ferro, 909 and 910 were showed tolerant to salinity. It is proven that crop growth decreases with increasing saline stress (Rouphael, et al., 2012). Hybrids of C. moschata cv. Koshk, C. pepo cv. Koshk and Asgharabad, Lagenaria Siceraria, Es113 and Cucurbita pepo con. Pepo var. Styriaca were sensitive to saline stress. Kumar et al, (2008) also reported plant response to salinity depends on type of salt, salt concentration and plant genotype.
Conclusions: Salinity stress adversely affect biomass and leaf water content of cucurbita. Our results indicated that local and hybrid cucurbit plants exhibited different response to saline stress. Hybrid rootstocks 909, 910 and Ferro were tolerant to salinity. Although local landrace C. maxima. cv. Shahreza and Kermanshah, C. moschata cv. Isfahan and C. pepo cv. Tiran were tolerant to salinity. Sensitivity to saline stress was similar between some local landrace and hybrid plants. Finally, after grafting in cucurbita rootstocks needs more research to use water, wast water, NaCl and other resources of salinity and to find the most tolerant rootstock.
Hassan Farhadi; Majid Azizi; Seyyed Hossein Nemati
Abstract
Introduction: Fenugreek (Trigonalla foenum-graecum L.), an annual herbaceous plant belonging to the Coleoidea (Fabaceae) family, has numerous medicinal properties such as decreasing blood glucose, laxative, appetizer, mucus, antipyretic and increasing the amount of milk during lactation . Among the most ...
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Introduction: Fenugreek (Trigonalla foenum-graecum L.), an annual herbaceous plant belonging to the Coleoidea (Fabaceae) family, has numerous medicinal properties such as decreasing blood glucose, laxative, appetizer, mucus, antipyretic and increasing the amount of milk during lactation . Among the most important problems in arid and semi-arid regions, drought stress or water shortage will have negative effects on plant growth. Drought stress occurs mostly because of reducing water availability in the soil. This may be due to excessive water loss or absorption problems, or both of them. One of the major factors limiting germination, which occurs in more arid and semi-arid regions, is salt stress. The study was done to evaluate germination of four Iranian population of fenugreek (Amol, Tabriz, Sari and Mashhad) under drought and salinity stresses.
Materials and methods: To investigate the effect of salinity and drought stresses on germination and seedling growth characteristics of native landrace fenugreek, two separate experiments were conducted in a Completely Randomized Design with three replications in Seed Laboratory of University of Mashhad in 2014. The experiment treatments consisted of four levels of salinity (0, 60, 120, 180 mM) that was induced by different concentrations of sodium chloride and drought stress induced by polyethylene glycol 6000 (PEG 6000) at four levels (0, -3, -6 and -9 Bar) with three replications. The drought stress levels were simulation by polyethylene glycol 6000 and using the Michel and Kaufmann formula. Distilled water was applied as control. Iranian seed population of fenugreek were purchased from the city of Amol, Tabriz, Sari and Mashhad then the seeds were washed with sodium hypochlorite (3 % v/v) for two minutes for disinfestation and washed three times with distilled water. On the twelfth day of experiment, seedling traits such as plumule and root length and weight in Petri dishes were measured.
Results and Discussion: The results of ANOVA showed that salinity and drought stress significantly (p≤0.01) affect germination process. The results of the means comparison confirmed that fenugreek germination in all seed population was reduced by increasing salinity levels. The lowest germination was detected under 180 mM salt stress in “Tabriz” population (12.04%), while the highest germination (95/26%) was calculated in “Mashhad” population at control. Seeds of “Mashhad” population in comparison to other population showed higher germination at other salinity levels. Germination rate also was significantly affected by salinity (p≤0.01). Decreasing of germination rate in fenugreek population of Amol, Tabriz, Sari and Mashhad under 180 mM salinity stress in comparison to control (0 mM) were 84/62, 87/80, 85/93, 82/59, respectively. Mashhad and Amol (second order) populations showed more germination rate stability after changes in salinity levels. The results of analysis of variance from this study showed that interaction effect of salinity and population significantly (p≤0.05) affect root length of the fenugreek. The root length of Mashhad and Amol populations were more stable at high level of salinity. Other researcher also reported that the root length decreased after salinity treatments (10, 13). Salinity stress affect water absorption by seed and decreasing Amylase and lipase activity caused degradation of stored material in seeds and then decreasing root length (23). Salinity, population and interaction between these factors significantly (p≤0.01) affected length of plumule. The results showed that Mashhad population had the highest and Tabriz revealed the lowest length of plumule. Interaction effects of treatments on seedling dry weight showed significant (p≤0.01) differences. Mashhad in comparison to the other populations had the highest seedling dry weight as 9.26, 8.10, 7.22 and 3.6 mg/seedling at different salinity treatments (0, 60, 120, 180 mM), respectively.
Interaction effects of drought stress and population on germination percentage was significant (p≤0.01). Mashhad and Amol (second order) populations were the best populations. At the highest level of drought stress (-9 Bar), these two populations had the lowest decrease in germination percentage. The mechanism of the results was the same as salinity which explained in above. According to the results of analysis of variance (ANOVA), all treatments and all interaction had significant effect (p≤0.01) on germination rate. The Mashhad and Sari populations were more tolerant than other populations against drought stress examined as germination rate as concerned. Analysis of variance showed that the simple effects of drought stress and population on root length were significant at one percent but interaction effect of drought stress and population was not significant statistically. The root length was in the range of 26.78 to 50.29 mm in the tested population. Mashhad population was more tolerant against drought stress in comparison to the other tested populations. Analysis of variance showed that simple effect of drought and population were significant (p≤0.01) on this trait but their interaction was not significant. Length of plumule mean comparison showed that the traite was in the range of 21.74 to 43.31 mm in Tabriz and Mashhad populations, respectively.Acoording to the length of plumule,Mashhad population in comparison to other tested populations under drought stress was tolerant . The water potential (drought stress), population and interaction between them showed significant effect (p≤0.01) on seedling dry weight. Among the evaluated population the Mashhad population was tolerant to drought stress when seedling dry weight was concerned. The highest correlation coefficient (r=0.96) was detected between germination percentage and root length and in second order the correlation coefficient between germination percentage and seedling dry weight was 0.93. The lowest correlation coefficient (0.61) was observed between germination rates and seedling dry weight.
Conclusions: The decline in germination percentage, germination rate, root length, shoot length and dry weight of seedlings with increased water osmotic potential in Mashhad population was less than other evaluated populations. Seed germination rate was more sensitive than seed germination percentage in both salt and drought stress therefore this trait is a suitable criterion for screening of tolerant population for future breeding purpose. The order for tolerance to salinity and drought stress in evaluated population was Mashhad, Tabriz, Sari and Amol.
Seyyedeh Mahdiyeh Kharrazi; Ali Tehranifar; Seyyed Hossein Nemati; Abdolreza Bagheri
Abstract
Introduction: Amaryllis is grown as pot outdoor plant and cut flower. Generally, this ornamental plant propagates by seed, suckers and scale cutting. Propagating by seed is not commercial and often used to produce new varieties. On the other hand, number of bulblets per mother bulb is very low under ...
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Introduction: Amaryllis is grown as pot outdoor plant and cut flower. Generally, this ornamental plant propagates by seed, suckers and scale cutting. Propagating by seed is not commercial and often used to produce new varieties. On the other hand, number of bulblets per mother bulb is very low under normal condition. Besides each bulb produces only 2 or 3 bulblets in a growing season and they become mature and produce flower stalk after 2 to 3 years. In some cases bulbs have no capacity to produce bulblet. Therefore, one of the strategies for shortening the growth period of the plant is to improve the traditional methods of plant propagations.
Materials and Methods: This study was done as a factorial experiment in a completely randomized design with 7 replications to evaluate the effects of medium and position of twin scales in mother bulbs on propagation of bulblets, in order to increase the rate of propagation of this ornamental plant. To measure wet and dry weight of explants, 3 replicates were used. For propagation, bulbs were cut radially into 12 equal pieces, so that each pieces were contained a part of the basal plate. To evaluate the effects of position of twin scales in mother bulbs, pieces were divided as twin scales and classified in 5 groups, so that the outermost twin scales was grouped in class 1 and the innermost twin scales was grouped in class 5. After that, the scale cuttings were dipped in 0.1 % carbendazim solution for 25 minutes and then surface water were dried using sterilized tissue paper. Media that used in this study were sand, perlite, vermiculite, Peat moss and cocopeat. For removing possible contamination from the media, all media were autoclaved for 30 minutes at 121 °C. Then twin scales cuttings were cultured in vented transparent plastic containers that filled with different media and were kept in a growth chamber at 25 °C and 16 hours lighting.Number of produced bulblet, bulblet diameter, root number, root length, fresh and dry weight of plants and browning rate of scales were recorded at the end of the experiment.
Results and Discussion: The results showed that medium and twin scale position in the mother bulb had a significant effect on the quality produced bulblet. The highest fresh weight of bulblet (1.58 g), bulblet dry weight (0.21 g) and the maximum diameter of the produced bulblet (1.5 cm) were obtained in the outermost twin scales and peat moss medium. Analysis of variance showed that the effect of culture medium on the number and length of produced leaf was significant (p
Masoud Azimi; Majid Azizi; Mohammad Farsi; Seyyed Hossein Nemati
Abstract
Introduction: Nowadays, mushroom and fungi are one of the most promising organisms which are used in biotechnology research (industry, medicine and agriculture). In the meantime, medicinal mushroom (mostly consumed as edible and medicinal products) have become a valuable biological resourcesin the pharmaceutical ...
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Introduction: Nowadays, mushroom and fungi are one of the most promising organisms which are used in biotechnology research (industry, medicine and agriculture). In the meantime, medicinal mushroom (mostly consumed as edible and medicinal products) have become a valuable biological resourcesin the pharmaceutical industry. Ganoderma the most legendary species of fungi in China with a long history dating back more than two thousand years.Ganodermalucidum (Fr.) Karst isa species belonging to the order of Aphyllophorales and family Basidiomycetes. The mushroom only growth on two or three types of trees among 10,000 known trees in the world and therefore is very rare. Ganoderma fruiting bodies and spores contain about 400 different bioactive compounds, which mainly includeTriterpenes, polysaccharides, nucleotides, sterols, steroids, fatty acids, proteins andpeptides. The mushroom polysaccharides, in addition to cancer treatment have showed antiviral properties, anti-inflammatory, anti-diabetic, anti-hypertensive and prevent blood clotting. Tavana et al (1) in the evaluation of the use of some agricultural and forest wastes material for production of the mushroom stated that the residue are suitable as a helpful supplements for the activity. Gonzalez-Matute et al (11) used sunflower seed shell after oil extraction as a substrate. They concluded that the sunflower seed shell can be used as the main energy source in the substrate to grow the mushroom. There are different agricultural wastematerials which are good sources for growing mushroom in our country. The use of agricultural residues has attracted much attention in recent years. To the best of our knowledge there are a few published studieson the production of Ganoderma in the field condition. This study was performed on Reishi mushroom (Ganodermalucidum) to investigate the effects of different agricultural wastes on some morphological characteristics (growth rate, fresh weight and dry weight of mycelia, biological yield andcrude polysaccharide content) and polysaccharide contents of fruits.
Material and Methods:The main portion of the medium for production of Ganodermalucidum was wood chips as 5-10 mm long that supplemented with different agricultural wastes included black seed waste, tea waste, hazelnut waste, coconut waste, almond wasteand sesame waste, with two types of bran (wheat and rice). The statistical design was afactorial experiment on the basis of completely randomized design with threereplications. The treatment were included
Wood chips (80 percent) + black seed waste (10 percent) + rice bran (10 percent)
Wood chips (80 percent) + tea waste (10 percent) + rice bran (10 percent)
Wood chips (80 percent) + sesame waste (10 percent) + rice bran (10 percent)
Wood chips (80 percent) + hazelnut waste (10 percent) + rice bran (10 percent)
Wood chips (80 percent) + coconut waste (10 percent) + rice bran (10 percent)
Wood chips (80 percent) + black seed waste (10 percent) + wheat bran (10 percent)
Wood chips (80 percent) + almond waste (10 percent) + wheat bran (10 percent)
Wood chips (80 percent) + sesame waste (10 percent) + wheat bran (10 percent)
Wood chips (80 percent) + hazelnut waste (10 percent) + wheat bran (10 percent)
Wood chips (80 percent) + coconut waste (10 percent) + wheat bran (10 percent)
At first Wood chips soaked in water for 2 days until the their moisture reached60-65 then the other agricultural waste materials added on the basis of the treatments and the autoclavable propylene bags filledwith the mixture and autoclaved for 2 hours at 121ºC. After cooling, all bags inoculated with wheat spawn of the Ganodermalucidium and the bags putunderdark condition in growth chamber with 85-95% humidity at 30ºC. After full colonization of the bags, they transfer to the light condition (200-500 Lux) at 25ºC until primordial formation. Then the light increased to 500-700 Lux until fruiting body formation.
Results and Discussion: The results of analysis of variance showed that the use of these agricultural wastes had a significant effect (P≤0.01) on growth rate, fresh weight, dry weight of myceliumand biological yield. The highest growth rate of mycelia (on the basis of days after inoculation to medium colonized completely) was detected in media enriched with tea waste, hazelnut waste, coconut waste and almond waste (15.33, 16.67, 15.33 and 14.33 days, respectively). The lowest growth rate of mycelium was detected in media enriched with black seed waste (30.33 days). The substrate supplemented with almond waste produced the highest amount of fresh fruit weight (31 g) and the lowest fresh fruit weight (15.74 g) was detected under coconut waste treatment. The highest amount of fruit dry weight (6.51 g) observed under the almond waste treatment and the lowest one observed under the coconut waste treatment (3.75 g). The media supplemented with almond wastes produced the highest biological yield (7.75%), but tea waste, hazelnut waste and coconut waste treatments had the lowest biological yield (4.75, 5.32, 5.27 and 3.93 percent, respectively) without significant differences (P≤0.01).
Seyyed Majid Zargarian; Ali Tehranifar; Seyyed Hossein Nemati; Bahram Syavashpor
Abstract
Introduction: In order to improve physical and mental human healthy, development of the urban area landscape is necessary. Water deficit is one of the most important problems in arid and semi- arid regions. Water deficit causes to reduce growth of different plants parts, including roots, aerial parts, ...
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Introduction: In order to improve physical and mental human healthy, development of the urban area landscape is necessary. Water deficit is one of the most important problems in arid and semi- arid regions. Water deficit causes to reduce growth of different plants parts, including roots, aerial parts, leaf area, height, dry weight, proteins, amino acids and chlorophyll. Using drought tolerance plantsisone solution. Moreover, one physiological method to decrease the hazardous effects of environmental stresses on plants is to applyplant growth regulators such as salicylic acid (SA). SA is one of the plant phenolic compounds in low amounts (mg/g wet weight or lower).
This hormone can perform an important role in resistance to stresses. Asteraceae is one of the biggest plant families with over 20,000 species which is distributed worldwide in moderate and tropical regions. A lot of border flowers of this family are widely used in landscaping. Toselect drought tolerance flowers and application of materials that reducing stress effects, this experiment was conducted to investigate the effects of salicylic acid on some morphological and physiological characteristics of five border flowers of this family.
Materials and Methods: The field experiment was conducted as split plot on randomized complete blocks design with four replications in which factors included two levels of water deficit (50, 100% FC) as main plot and two levels of SA (0, 1mmol/L-1) as sub plot in the horticulturedepartment of faculty of Agriculture atFerdowsi University of Mashhad. In this investigation five border flowers (Ageratum houstonianum‘Blue Danube’, Tagetserecta ‘Discovery Yellow’, Tagetspatula‘Hero Harmony’, Zinnia elegans ‘Lilliput rose’andCallistephuschinensis ‘Milady Mix’ ) of Asteraceae family were chosen for study. The soil mixture was analyzed before starting the test. According to the result of physicochemical analysis of the soil, it had loamy texture (52% sand, 30% silt and 18% clay) with 7.04 pH and 3.6 ds/m electrical conductivity (EC) and total N (0.175mg/kg), P (142.7 mg/kg) and K (142.3mg/kg) were reported, too. Investigated characteristics were height of the plants, leaf area,flower number,root dry weight, flower dry weight, aerial parts dry weight,chlorophyll a, chlorophyll b, ,total chlorophyll,carotenoide,stomata conductance,relative water content (RWC) and electrolyte leakage. Tomeasureleaf area and stomata conductance, leaf area meter and prometer were used, respectively. In addition, electrolyte leakage, RWC, chlorophyll and carotenoide were evaluated by Sairamet al., Smart and Bingham, Arnon and Rangan methods, respectively. Minitab 16 software was used for data analysis and LSD test (p≤ 5%) for mean comparison was applied.
Results and Discussion: The results of this study showed that all characteristics of examined flowers decreased underwater deficit treatment (50% FC). SA had different effect on flowers properties. Although SA had not significant effect on flower number, it improved the percentage of characteristicssuch asflower dry weight, chlorophyll a, stomata conductance and RWC of Ageratum houstonianum; root dry weight, carotenoid andstomata conductance of Zinnia elegans; carotenoid of Tagetserecta; flower number, flower dry weight, carotenoid and stomata conductance of Tagetspatula; root dry weight, flower dry weight, aerial parts dry weight andstomata conductance of Callistephuschinensis. Moreover, SA had significant effect on the considered characteristics of the other studied flowers. Interaction effect of these two factors had different effects on all of the examined characteristics of five flowers. The highest amounts of the investigated characteristics mostly obtained from water deficit with SA application treatment. Under water deficit, SA could influence leaf area and electrolyte leakage of all examined flowers and aerial parts dry weight of all flowers except Callistephuschinensis. Among the studied flowersTagetserectaand Zinnia elegans showed the highest and Callistephuschinensisdisplayed the lowest response to SA application under water deficit. Underwater deficit conditions SA probably cause to improve nutritious elements that can increase plant growth. Different effects of SA on morphological and physiological properties may be due to the plant species and environmental conditions that can influence on morphophysiologic process variously.
Conclusions: The results of this studyindicated that SA resulted in the improvement of themost of morphological and physiological properties. However different results obtained,due to the variation in morphological and physiological characteristics of flowers.
Helale Kaboli Farshchi; Majid Azizi; Seyyed Hossein Nemati; Vahid Rowshan
Abstract
Introduction: Medicinal and aromatic plants can play an important role in commercial crops, which also represent a safe alternative for chemical pharmaceutical industries. St. John’s Wort (Hypericum perforatum L.) due to its therapeutic efficacy has been used for decades in folk medicine and is considered ...
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Introduction: Medicinal and aromatic plants can play an important role in commercial crops, which also represent a safe alternative for chemical pharmaceutical industries. St. John’s Wort (Hypericum perforatum L.) due to its therapeutic efficacy has been used for decades in folk medicine and is considered as a promising medicinal plant with valuable potential as a source of hypericin, essential oils and antioxidants. Studies on agronomic factors such as application of potassium and humic acid as well as nitrogen fertilization on yield, essential oil and antioxidant activity of Hypericum perforatum have not been investigated thoroughly until now. This study was designed to study the effect of using humic acid and potassium sulphate on morphological and phytochemical characteristics of Hypericum perforatum.
Materials and Methods: The plant material was prepared from Science and Technology Park in Khorasan-e Razzavi–Mashhad. In spring, the plants were transplanted into the field of Horticulture department, Ferdowsi University of Mashhad, for fertilizing treatments. The experimental layout was factorial in a complete randomized design (CRD), with three replications. Potassium Sulphate (K2SO4) was applied at the rates of 0.0 (K0), 60 (K60) and 100 (K100) kg ha-1. The other treatment were humic acid, which was applied at three rates 0 (H0), 20 (H20) and 40 (H40) L ha-1. Plants were treated at two stages, before flowering by potassium fertilizer and fertigated four times in 15-day intervals with humic acid. Besides the fresh and dry weight, number of flowers, stem height and number of flowering stems were determined at the end of the growing stage.
Results and Discussion: The ANOVA indicated that most of measured attributes of Hypericum perforatum plant were significantly affected by both treatments. Data presented revealed that the highest stem (91.6 cm) was recorded at the highest level of potassium sulphate (K100) treatment, while the shortest height (60.4 cm) was recorded at the lowest level of potassium sulphate (K0) treatment. On the other hand, the highest stem (80.33 cm) was achieved with the highest level of humic acid treatment. No significant difference was observed between the highest level of humic acid (H40) and the second level (H20), while both mentioned levels showed a significant difference with control (H0). This means that the control plants possessed the lowest stem height (66.7 cm). The DPPH method for evaluation of antioxidant activity reflects the ability of the present compounds to scavenge hydrophilic free radicals. DPPH inhibition was investigated and the results were evaluated as relative activities against α-tocopherol as positive control. A lower EC50 indicates a higher antioxidant activity. As it is shown, the least EC50 which indicates the highest antioxidant activity belonged to plants treated with potassium sulphate at (100 kg.ha-1). Control plants possessed the least antioxidant activity and showed no significant difference compared with plants treated with 60 kg.ha-1 of this fertilizer. Humic acid had a significant effect on antioxidant activity of H. perforatum. The lowest value of EC50 was related to the highest level of humic acid, while the highest value of EC50 was obtained from control plants. There was also significant difference between control and two other levels (20 and 40 L ha-1) of humic acid. Applied fertilizers at their high levels showed significant effects on EC50 decrease, which means the increment of antioxidant activity of H. perforatum. The high antioxidant activity of this medicinal plant is the reason of flavonoids and phenols existence. It seems that application of these fertilizers have led to increment of the mentioned secondary metabolites and followed by an increase of antioxidant activity in comparison with control plants. Totally, H. perforatum possessed a high antioxidant activity. Based on recorded data of the present study, application of the mentioned fertilizers separately or simultaneously caused the increase of this important medicinal plant fresh and dry weight, and finally the yield. Number of flowers, stem height and flowering stems reached the highest value at the highest level of both fertilizers (K100H40). As potassium plays an important role in the development of reproductive parts of plants, it might be one of the reasons of the above mentioned increase. The highest essential oil content was recorded at high levels of both fertilizers (K100H40). Since the highest essential oil content accumulates in the flowering stems, an increase of these stems height and a higher number of flowers would lead to an increase of essential oil content of H. perforatum. Antioxidant activity of this plant is flavonoids contributed. It seems that application of these fertilizers increased the antioxidant activity by an increase of this pigment content.
Sonayye Beigi; Majid Azizi; Seyyed Hossein Nemati; Vahid Rowshan
Abstract
Introduction: Medicinal plants are rich in active substances and primarily have been used in the manufacture of many drugs. Basil (Ocimum basilicum L.) is one of the important medicinal plants whichbelongs to the Lamiaceae family. Basil essential oil content (between 0.5 to 1.5 percent) varies according ...
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Introduction: Medicinal plants are rich in active substances and primarily have been used in the manufacture of many drugs. Basil (Ocimum basilicum L.) is one of the important medicinal plants whichbelongs to the Lamiaceae family. Basil essential oil content (between 0.5 to 1.5 percent) varies according to climatic conditions of habitat location. Basilneeds a lot of water during growth period and it is very sensitive to water stress and shows wilting symptoms very soon after water shortage. Iran is located in an arid and semi-arid region which has little precipitation that is not enough for crop water requirements. Nowadays, the use of superabsorbent polymers is one of the ways to create sustainable agriculture and increase irrigation efficiency. They can store high water or aqueous solutions in root zone of plants and to reduce negative effects of drought stress. So, improvement of plant growth, increasing of irrigation intervals, reducing water loss and costs of irrigation is due to the application of superabsorbent polymers. Mucilages are also the herbal polysaccharides, soluble in water, and commonly include carbohydrates and can be used as hydrophilic polymers. The aims of this investigation were to study the effects of hydrophilic polymers on water use efficiency, morphological characteristics (dry matter, leaf area, and leaf number), essential oil quantity and yield of basil to harden plant to drought stress and to evaluate its potential to cultivate in arid regions. In addition, taking steps forward towards sustainable agriculture, by reducing the cost of agricultural production, helps protecting the environment.
Materials and Methods: This research was conducted as a pot experiment at the department of Horticultural Science‚ college of Agricultural‚ Ferdowsi University of Mashhad‚ Iran, during 2012-2013.The research was set out in a factorial experiment on the basis of completely randomized block design with three replications.Two hydrophilic polymer Stockosorb® (industrial) and malva leaf (herbal) with two application methods (mixed with soil, mixed with soil+root) at 4concentrations (0, 0.1%, 0.2% and 0.3% w/w) were used. Leaf number, leaf area, dry matter yield, water use efficiency, essential oil quantity and yield were measured. Improved seeds of Ocimum basilicum var. Keshkeniluvelou were sown and seedlings were transplanted to the pots in four-leaf stage. Hydrogels were prepared and mixed with water after weighing the polymers according to determined concentration. After establishment, all pots were irrigated with a determined amount of water after reaching to the highest concentration (0.3%) of the wilting point. So, in this state in addition to the treatment at 0.3% treatment, lower levels (0.2% and 0.1%)were reached to wilting point earlierand were exposed to water stress.
Results and Discussion: Superabsorbent polymers can by absorption of irrigation and rain water, prevent deep percolation of water and with decreasing irrigation interval and reduced water consumption and increase water use efficiency. The results showed that using Stockosorb superabsorbent polymer and malva leaf as herbal hydrophilic polymersare useful for water supply of the plant in water stress.Application of these substances on morphological characteristics, water use efficiency and basil oil, were superior to the control.According to the obtained results, malva leaf use in soil and roots in treatmentless than (0.1% and 0.2%)number and leaf area and higher than (0.3%)increased dry matter yield, water use efficiency and the essential oil quantity and yield. Stockosorb (0.2%) byboth applying methods increased dry matter yield and water use efficiency. Also Stockosorb (0.2%),soil application increased leaf number and area. The essential oil yield and quantity increasedwith use of Stockosorb in soil + root in for0.2% and 0.3% treatments, respectively.
Conclusion: Due to the importance of cultivation of medicinal plants to provide conditions to increase essential oil yield and quantity at the same time, the best method used were malva leaf and Stockosorb with concentrations higher than (0.3%) and soil application methodor the use of both polymers with application method in soil + root in the moderate concentration (0.2%).For that purpose, we obtained favorable conditions for improving the morphological characteristics, water use efficiency and increasing the quantity and of essential oil yield in drought stress simultaneously. The results showed that natural compounds can be a good alternative for chemical compounds, but further experiments are needed for their commercialization.
