Pomology
Akram Heydari; Saeid Daghighi; Farhad Azarmi-Atajan
Abstract
Introduction Pistachio (Pistacia vera L.) is an important crop in Iran our country and has a unique position in export goods. The amount of pistachio production in Iran has decreased by 50% compared to 2017. The low yield of pistachios per unit area is due to the management problems of orchards, ...
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Introduction Pistachio (Pistacia vera L.) is an important crop in Iran our country and has a unique position in export goods. The amount of pistachio production in Iran has decreased by 50% compared to 2017. The low yield of pistachios per unit area is due to the management problems of orchards, and among these, nutrition and fertilizer management is of special importance. Among the nutrients that are important in pistachio nutrition, especially when the brain is full, are nitrogen and potassium. Humic acid can also improve physical, chemical and biological soil properties and stimulate growth via its effects on plant metabolism.The aim of this study was to investigate the effect of application of some nitrogen fertilizers with different levels of potassium sulfate and humic acid on growth, yield and photosynthetic pigments of pistachio Badami Sefid-e-Mahvalat variety. Materials and Methods This research was conducted as a factorial experiment based on a randomized complete block design with three replications at the Mahvalat during 2019-2020. The first factor consisted of nitrogen fertilizers at 4 levels (control, urea, ammonium sulfate and ammonium nitrate). The second factor was potassium sulfate fertilizer at 2 levels of zero and 250 g per tree and the third factor was humic acid fertilizer at 2 levels of zero and 45 g per tree which as a manure pits after the formation of the cluster and at the same time with the growth of the bony shell in the shade of the tree where the capillary roots are active. At the end of the experiment, morphophysiological traits were measured and recorded. In each tree, three branches were selected in different directions and the length of the current branch was measured in meters using centimeters. The diameter of the middle of the branch was measured with a caliper. From the collected clusters, 100 fruits were randomly selected, and the number of indehiscence fruits and the number of blank fruits were counted and finally expressed as a percentage. Measurements of chlorophyll a and b, total chlorophyll and carotenoids were determined using Arnon method. The experimental data was analyzed by SAS software and the significant differences among the treatment were tested by LSD test. Results and DiscussionThe results of analysis of variance of data in two years of experiment showed that experimental treatments had a significant effect on pistachio growth and yield. The results of the first year showed that the combined treatment of urea and 250 g of potassium sulfate and 45 g of humic acid had the highest diameter and branch length, indehiscence percentage, chlorophyll a, b and total and the lowest pistachio blank percentage. The highest yield in the first year was obtained from the combined use of ammonium sulfate, 250 g of potassium sulfate and 45 g of humic acid. Combined application of ammonium nitrate and 250 g of potassium sulfate and 45 g of humic acid resulted in the highest diameter and branch length in the second year of the experiment. The highest indehiscence percentage and carotenoids and the lowest amount of pistachio blank percentage in the second year were obtained from the combined treatment of ammonium sulfate, 250 g of potassium sulfate and 45 g of humic acid. Most chlorophyll a, b and total were obtained from combined consumption of urea, 250 g of potassium sulfate and 45 g of humic acid. The highest yield of the second year was obtained due to urea consumption, 250 g of potassium sulfate and 45 g of humic acid. Nitrogen is a component of amino acids, proteins, nucleic acids and enzymes and plays a major role in plant physiology, vegetative growth, chlorophyll formation and fruit and fruit production. Potassium is also one of the elements required by the plant that plays an important role in photosynthesis and transport of carbohydrates. The organic acids in humic acid cause the chelating of many nutrients and increase their availability to the plant. By using these substances and its positive and stimulating effects on plant growth and increasing root growth and its absorption power, nutrient uptake, yield is increased.
Pomology
Mohammad Ali Ahmadi; Saeid Daghighi; Farhad Azarmi-Atajan; Hassan Bayat
Abstract
Introduction
Jujube (Ziziphus jujuba Mill.) as one of the invaluable medicinal plants, is widely used in the pharmaceutical industry due to its vitamins, mucilage, antioxidants, alkaloids and phenolic compounds. South Khorasan province is the most important area of jujube fruit production in Iran. Optimum ...
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Introduction
Jujube (Ziziphus jujuba Mill.) as one of the invaluable medicinal plants, is widely used in the pharmaceutical industry due to its vitamins, mucilage, antioxidants, alkaloids and phenolic compounds. South Khorasan province is the most important area of jujube fruit production in Iran. Optimum supply of nutrients in fruit trees can increase fruit quality. Calcium is one of the most important elements that can affect quantity, quality, storability and marketability of many horticultural crops. Due to the relatively high amount of lime in the agricultural soils of South Khorasan province, the amount of calcium in these soils is almost high. However, due to low mobility of calcium from soil to plant, calcium deficiency is common in horticultural plants. Also, potassium is required for cellular osmotic and ionic balances, electrochemical processes, neutralization of organic acids, regulation of stomatal function, cell division, enzyme activation, protein synthesis, as well as the synthesis and translocation of sugars. Foliar spraying is an effective way to supply nutrients needed by plants, especially in calcareous soils with high pH. In this method, nutrients are directly available to different parts of the plant in a short period of time. Studies have shown that leaf feeding with calcium and potassium can be useful for increasing the qualitative characteristics of fruit in most products, especially in arid and semi-arid regions. Nevertheless, little information is available regarding the foliar application of this element on jujube plant. Hence, the current research was aimed to investigate the effects of foliar application of calcium chloride and potassium sulfate on physicochemical properties and quality of fresh jujube fruit.
Materials and Methods
In order to investigate the effect of foliar application of calcium chloride and potassium sulfate on the physical and biochemical properties of the fruit of the jujube plant, two separate factorial experiment was conducted in the form of a randomized complete block design in three replications in two areas of Siojan and Mazhan, Khosef city in 2022. Experimental treatments included calcium chloride (0, 0.5 and 1%) and potassium sulfate (0, 0.1 and 0.3%). Fresh fruit was harvested at a crisp mature (white-red) stage mid in August. Data were analysed using SAS software (ver. 9.4) and comparison of means was made with LSD test at 1 and 5% probability.
Results and Discussion
The results of analysis of variance showed that calcium chloride foliar application in Siojan garden had significant effect on the physical and biochemical characteristics of jujube fruit. So that the highest fresh weight (4.07 gr), dry weight (1.89 gr), length (29.11 mm), diameter (21.52 mm) and texture firmness (18.81 N/cm2) of fruit were obtained from the level of 0.5% calcium chloride. Also, the foliar spraying treatment with 0.5% calcium chloride had the highest amount of carotenoid and total phenol in the fruit. The highest amount of calcium and potassium in the fruit was obtained from the level of 1% calcium chloride. The effect of foliar application of calcium chloride on physical and biochemical properties of jujube fruit in Mazhan garden was significant. In Mazhan garden, the highest fresh weight (3.9 gr), dry weight (1.37 gr), length (28.41 mm), diameter (20.91 mm) and texture firmness (18.61 N/cm2) of fruit were obtained from foliar application of 0.5% calcium chloride. The highest amount of carotenoids (0.443 mg/100 g of fresh weight) and phenol (9.53 mg of gallic acid per 100 g of fresh weight) in Mazhan garden was obtained from the level of 0.5% calcium chloride. The content of calcium and potassium of jujube fruit in Mazhan region was maximized with the application of 1% calcium chloride. The results showed that the application of potassium sulfate in Siojan garden had an significant effect on the physical and biochemical characteristics of the fruit. The highest fresh weight (3.63 gr) and dry fruit weight (1.89 gr), length and diameter and texture firmness of fruit was obtained from the level of 0.3% potassium sulfate. Also, the highest amount of biochemical indices of jujube fruit in Siojan garden was obtained from the level of 0.3% potassium sulfate. The interaction effect of foliar application of calcium chloride and potassium sulfate on fresh and dry fruit weight, fruit length and carotenoids of fruit in Siojan garden was significant. In Mazhan garden, the fresh and dry weight of fruit, amount of carotenoids and total phenol of fruit were affected by the interaction effect of experimental treatments, and the highest level of these characteristics was obtained from the level of 0.5% calcium chloride and 0.3% potassium sulfate.
Conclusion
The results of foliar spraying of calcium chloride and potassium sulfate in both studied orchards showed that these treatments, both solely and in combination, had a positive and significant effect on various characteristics of jujube fruit. So the highest physical and biochemical characteristics of the fruit were obtained from the simultaneous application of 0.5% calcium chloride and 0.3% potassium sulfate. Therefore, Based on the results of this research, foliar spraying 0.5% calcium chloride and 0.3% potassium sulfate can have an effective role in increasing the quantity and quality of jujube fruit.
Medicinal Plants
Saeid Daghighi; Farhad Azarmi-Atajan; Nasibeh Chopani Aghech
Abstract
Introduction
Barberry is one of the important agricultural products of Iran and has an important role in the economy of farmers, especially in South Khorasan province. Salinity as abiotic stress can cause an ionic or osmotic imbalance in plant cells. Salt stress also restricts plant growth and ...
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Introduction
Barberry is one of the important agricultural products of Iran and has an important role in the economy of farmers, especially in South Khorasan province. Salinity as abiotic stress can cause an ionic or osmotic imbalance in plant cells. Salt stress also restricts plant growth and development by affecting water reducing availability and affecting plant production. Despite the relatively high tolerance of barberry to environmental stresses, increasing soil salinity and irrigation water in barberry growing areas, the growth, and yield of this agricultural product have decreased. The use of plant growth-promoting rhizobacteria (PGPR) is a new method that has been shown to increase the tolerance of various plants to salinity stress.
Materials and Methods
Due to the lack of information about the effect of salinity on the growth and establishment of barberry off-shoot and the role of beneficial soil bacteria in increasing the tolerance of this plant to salinity stress, this study aimed to investigate the role of bacteria on growth, physiological and biochemical properties and uptake of nutrients by barberry off-shoot at different levels of irrigation water salinity. For this purpose, a factorial study was conducted in a randomized complete block design with 3 replications. Experimental factors included plant growth-stimulating bacteria at three levels (control (Without inoculation) and inoculation with Pseudomonas sp. P1 and Pseudomonas sp. P2) and salinity of irrigation water at three levels (control, 6 and 12 dS/m from sodium chloride source). The bacteria used in this study were able to produce indole acetic acid, siderophore, ACC deaminase enzyme, and dissolve insoluble phosphate (tricalcium phosphate) in vitro. For inoculation, inoculum containing each bacterium with a population of 108 cells/ml was prepared in the Nutrient Broth medium and added to the root medium. The plants were irrigated with non-saline water for one month and then with saline water for two months based on experimental treatments. Finally, leaf sampling was performed and various characteristics such as leaf dry weight, chlorophyll, proline, total sugar, RWC and phosphorus, potassium, sodium, and chloride concentrations were measured. Analysis of variance of traits was performed using SAS software and the means were compared using the LSD method with a probability level of P≤0.05.
Results and Discussion
The results showed that the salinity of irrigation water reduced leaf dry weight, chlorophyll and carotenoid concentration, relative water content, and potassium to sodium ratio of barberry leaves. Decreased photosynthetic pigments under salinity may be due to decreased synthesis of the main chlorophyll pigment complex, oxidative damage to chloroplast lipids, pigments, and proteins, or increased chlorophyllase activity. In contrast, with increasing salinity, the amount of proline and total sugar and the concentration of phosphorus, sodium, and chlorine in leaves increased. Bacterial inoculation also increased leaf dry weight, chlorophyll, carotenoids, potassium concentration, relative water content, and potassium to sodium ratio, especially in saline conditions. Also in saline conditions, the concentrations of sodium, chlorine, phosphorus, proline, and total sugar in the leaves of barberry off-shoot inoculated with bacteria decreased. It seems that PGPR plays a significant role in the regulation of cellular osmolites, including proline and soluble sugars, by producing various metabolites and increasing the absorption of water and nutrients. The highest amount of leaf dry weight (0.70 g), total chlorophyll (0.92 mg g-1 fresh weight), carotenoids (0.51 mg g-1 fresh weight), leaf potassium (0.48 %), and total leaf sugar (43.7 mg g-1 dry weight) was obtained from the application of PGPR in conditions without salinity stress. Also, the use of bacteria in saline conditions decreased the amount of phosphorus and total sugar and in non-saline conditions increased the amount of these parameters. PGPR through various mechanisms such as the production of auxin, organic and mineral acids, and secretion of proton and phosphatase enzymes increase the availability of phosphorus for the plant, root growth, and absorption of water and nutrients. Increased absorption of water and nutrients has led to increased leaf growth and development and therefore reduced phosphorus concentration (dilution effect).
Conclusion
According to the results, PGPR by increasing the absorption of water and nutrients such as phosphorus and potassium caused osmotic regulation in the plant and thus increased the tolerance of barberry off-shoot to salinity stress of irrigation water. The ability of these bacteria to improve plant growth in saline conditions could be due to the production of auxin, siderophore, dissolution of tricalcium phosphate, and especially the production of the enzyme ACC-deaminase (as observed in vitro). Therefore, these bacteria can be used to improve the nutrition growth and establishment of barberry off-shoot.
