Ahmad Reza Bolandi; Hassan Hamidi; Roya Beidokhti
Abstract
The experiment was conducted to study the effect of photoperiod and hormones on production of microtubers for two potato cultivars (Sante and Savalan), which were clean and pathogens free. This experiment was performed as a factorial experiment based on randomized design with three replications. The ...
Read More
The experiment was conducted to study the effect of photoperiod and hormones on production of microtubers for two potato cultivars (Sante and Savalan), which were clean and pathogens free. This experiment was performed as a factorial experiment based on randomized design with three replications. The results showed that cultivar, hormone and photoperiod would effect on all studied traits. Sante cultivar indicates more efficiency for all measured traits than in Savalan cultivar. In this experiment by using the combination of two hormones 2, 4-D and BAP would increase number, diameter and weight of microtubers. The means comparison of photoperiod showed that highest efficiency for all traits as treatment of plant for 8 hours in darkness plus 16 hours in light plus utter darkness (P3). In this research, the highest number of microtuber was related to cultivar of Sante (9.47) is gained with hormone 2, 4-D, as well as photoperiod P3 which this superiority for Savalan cultivar is gained by using the combination of two hormones and P3 photoperiod.
Ebadollah Ebadi; Ahmad Reza Bolandi; Hassan Hamidi; Jamshid Moaven; Hassan Hassan Abadi
Abstract
Potato (Solanum tuberosum L.) ranks the fourth most important food crops in the world. Determination of genetic diversity using different markers have a key role in plant breeding programs. In this research, SDS-PAGE technique was used as a tool for assessing genetic diversity relationships among 23 ...
Read More
Potato (Solanum tuberosum L.) ranks the fourth most important food crops in the world. Determination of genetic diversity using different markers have a key role in plant breeding programs. In this research, SDS-PAGE technique was used as a tool for assessing genetic diversity relationships among 23 potato cultivars (Solanum tuberosum L.). Potato tubers proteins were extracted using Laemmli method (1970). UPGMA method and NTsys softwar were applied to calculate polymorphism and to draw dendrogram. Genetic distance of samples was calculated by SPSS softwar. 39 protein bands were observed in populations under study. The results revealed maximum number of bands (23 bands) in Markiz cultivar. Cluster analysis differentiated 23 cultivars in two large groups. Dendrogram constructed shows that Granola is closest to Impala (73.7%). Also, Fontaneh and Savalan cultivars were the most dissimilar ones (9.1%). Two major group of storage proteins appeared at 22 and 40 kDa positions could be probably the isomers of the Sporamin and Patatin, respectively. The results indicate presence of high level of genetic diversity in potato cultivars.
