Mohsen Alipoor; Mohammad Farsi; Amin Mirshamsi Kakhki
Abstract
The white button mushroom, Agaricus bisporus, is a commercially important cultivated filamentous fungus. Strain stability is of great importance to both spawn producers and mushroom growers. Mushroom strains are usually propagated via vegetative method on nutritionally rich substrates. Abnormal growth ...
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The white button mushroom, Agaricus bisporus, is a commercially important cultivated filamentous fungus. Strain stability is of great importance to both spawn producers and mushroom growers. Mushroom strains are usually propagated via vegetative method on nutritionally rich substrates. Abnormal growth and poor yield are the consequences of this replication method. The reason for this phenomenon is still unknown. The use of molecular markers is one way of assessing and understanding the genetic changes. In this study, for the first time we reported the application of amplified fragment length polymorphism (AFLP) marker to assess genetic variation in single spore progeny and to assist selection of superior cultivars based upon the use of monosporous cultures of A. bisporus commercial strain, Holland737. We isolated 30 single spores that differed in growth rate, productivity and AFLP inheritance pattern. Nine EcoRI / TaqI primer combinations identified a total of 353 AFLP bands from 19 single-spore isolates, of which 53 were polymorphic. Results showed that the single spore selection is an effective method for strain improvement in A. bisporus, so that two isolates averagely performed 47% increased yield over the maternal strain and AFLP showed enough sensitivity to detect polymorphisms among single spore isolates.
Shirin Rezaei; Amir Lakzian; Mohammad Farsi; Mahboobeh Abolhassani-Zeraatkar; Gholamhossein Haghjnia
Abstract
One of the most important steps of Agaricus bisporus production is casing. In this step a layer of soil is added on top of compost. Peat is the most suitable casing soil for A. bisporus production. The lack of peat in Iran is one of the major problems in A. bisporus production for mushroom producers. ...
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One of the most important steps of Agaricus bisporus production is casing. In this step a layer of soil is added on top of compost. Peat is the most suitable casing soil for A. bisporus production. The lack of peat in Iran is one of the major problems in A. bisporus production for mushroom producers. It seems that peat can be replaced by Spent Mushroom Compost (SMC). In order to study the possibility of peat replacement, a factorial experiment was conducted in a completely randomized design layout with two replications. The experimental factors consisted of Spent Mushroom Compost with two levels (one and two years old), leaching with three levels (one (L1), two (L2) and three (L3) times) and EDTA with two levels (without EDTA (E1) and with EDTA, 0.3 M (E2)) and different casing soil with four levels (SMC+loam, SMC+Azolla, SMC+Peat (1:1) and Peat). The experiment was carried out in the Mushroom Production Center of Agricultural College, Ferdowsi University of Mashhad. The results showed that the highest mushroom yield was obtained in peat treatment (24%). The average of yield in SMC+Peat treatment was 11.78%. The Diameter of mushroom cap in SMC+Peat treatment was 44.1 mm. The lowest yield (8.1%) and diameter of A. bisporus cap (37 mm) was observed in SMC+Azolla treatment. The results also showed that the EDTA had a negative effect on yield (44.3 %) and diameter of the mushroom cap.
