Pomology
M. Ghazaeian; D. Hassani; S. Zamani; M. Adibi
Abstract
Introduction
The Juglandaceae family includes about 50 species of 11 genera of which Carya (hickory tree), Pterocarya (wingnut tree), and Juglans are the major members. The pecan, Carya illinoinensis is the most economically important member of the Carya genus and is the most valuable native North American ...
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Introduction
The Juglandaceae family includes about 50 species of 11 genera of which Carya (hickory tree), Pterocarya (wingnut tree), and Juglans are the major members. The pecan, Carya illinoinensis is the most economically important member of the Carya genus and is the most valuable native North American nut crop. The Carya genus comprises 20 species.
Materials and Methods
Golestan province is located in the northern temperate region between 37.2898° N, 55.1376° E from the Greenwich meridian and in the northern part of the country. The amount of precipitation reaches 200 mm in the northernmost part of the province and more than 700 mm in the southern parts of Alborz foothills. Temperature varies across different parts of the province, generally increasing from west to east and from south to north. The genotypes used in this project are G4, G3, G43, G63, which were selected and propagated from the elite trees available in the province after quantitative and qualitative evaluation, and the cultivars are Mahan and Comanche, which were propagated from grafted trees of the Dezful Research Center collection. Grafted seedlings were produced using thethermal cable technique and planted in the specified locations in the above 2 regions. Grafted trees from 4 elite genotypes of Golestan (G4,G3,G43,G63) plus Comanch and Mahan 2 commercial cultivars have been studied in randomized completely block design (RCBD) for 3 years in two regions. In the second phase of experiment, the fruit characters and yield evaluated based on pecan descriptor. The results were analyzed by SAS statistical software and the reactions of genotypes were evaluated in different places and during different years.
Results and Discussion
This evaluation was conducted during the years 2021 to 2024 at Chalki research station of the Golestan Agricultural and Natural Resources Research and Education Center in Gorgan and on the farmer's land located in Igder village, located 30 km from Gonbad city. Measurements were conducted on fruits harvested in 2022 and 2023. During both years, the Mahan cultivar did not produce a sufficient number of fruits in either region and was therefore excluded from the comparison. A significant effect of location and year was observed only for the fruit weight trait. Among the genotypes, G43 exhibited the highest fruit weight (11.75 g) and demonstrated vigorous growth with a broad canopy in both locations. However, fruit yield over the two years of evaluation showed no significant differences among the studied cultivars and genotypes across the two regions.Due to the results of the first phase, the maximum tree height was in Mahan (242.5 cm) and the lowest height in G63 (118.66 cm). The maximum canopy spread was in comanch (138.72 cm) and the lowest was in G63 (90.11 cm).There were significantly differences between two regions. The length of the growing season among cultivars was 257 days in Gonbad and 237 to 247 days in Gorgan. Study of dichogamy during the years 2021-2022 in the cultivars and genotypes showed two cultivars Comanche and Mahan were protandry in Gorgan, while both cultivars showed protogyny in Gonbad. Also, 4 genotypes of the province (G3, G4, G43, G63) showed protogyny.
Conclusions
This project, conducted over both vegetative and reproductive phases, evaluated elite pecan germplasm within the province. The objective was to utilize the region’s existing genetic potential and to compare it with commercially available cultivars in the country, with the goal of identifying and promoting superior genotypes for the establishment and expansion of new orchards in Golestan Province. The results of the two phases of this research showed that some of the introduced genotypes have the ability to compete with the existing commercial cultivars, which will require further investigation in the years of peak fruiting and maturity of the tree. The length of the growing season among cultivars was 257 days in Gonbad area and 237 to 247 days in Gorgan. Also, the effects of drought conditions in Golestan province during 2021-2022 should be included in the relative determination of the results. As a summary, based on the evaluation of these two phases and considering the growth and fruiting conditions, two genotypes G4, G43 and G63 can be considered as promising genotypes for development.
Acknowledgement
The authors consider it necessary to appreciate the support of Horticultural Sciences Research Institute (HSRI) and Golestan Agriculture and Natural Resources Research and Education Center in the implementation of this project.
Abbas Joulani; Yavar Sharafi; Javad Sabbaghpour Heris
Abstract
Introduction: Sour cherry (Prunus cerasuc L.) is one of the most important stone fruit of Rosaceae family which have a high nutritional value. Based on FAO statistics, Iran was ranked the 3rd to 6th among the main sour cherry producer countries in the world. Most of the sour cherry cultivars are self-compatible, ...
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Introduction: Sour cherry (Prunus cerasuc L.) is one of the most important stone fruit of Rosaceae family which have a high nutritional value. Based on FAO statistics, Iran was ranked the 3rd to 6th among the main sour cherry producer countries in the world. Most of the sour cherry cultivars are self-compatible, but self-incompatible cultivars have been reported currently. Allotetraploidy of sour cherry complicates the self-incompatibility segregation in the progeny, so this studies have been less accomplished in Iran. The sour cherry is an allotetraploid species which possibly stand up as a result of usual hybridization between the dwarf cherry P. fruticosa and sweet cherry P. avium. Similar to other species of the Prunus, the phenomenon of gametophytic self-incompatibility (GSI) happens in sour cherry. It prevents self-fertilization and facilitate fertilization with pollen of other genotypes within same species. Self-incompatibility consists of arresting pollen-tube growth in the pistil if the pollen tube contains the same S-allele that is present in the pistil. The phenomenon of self-incompatibility in plants of Rosaceae family is controlled by two genes at the S-locus. The specificity of the pistil is encoded by a gene responsible for the synthesis of the ribonuclease protein (S-RNase), whereas the specificity of the pollen is controlled by the gene encoding an F-box protein (S-haplotype-specific F-box protein – SFB) 3a). The tightly linked genetic unit of the pistil S-allele (SRNase) and pollen S-allele is called S-haplotype. Materials and Methods: In the present study, pollen-pistil compatibility relationships among some sour cherry cultivars and selected genotypes were investigated by controlled pollination and pollen tube penetration by fluorescence microscopy. Cultivars were include ‘Meteor’, ‘Mont Morency’, ‘Majarestani’ and ‘Mahalli’ so, genotype1 were selected. After controlled pollination fruit setting was studied and the pollen tubes growth along the style was studied using fluorescent microscope. Crosses were selected based on synchronized flowering time among cultivars and genotypes and included ‘Mont Morency’ × ‘Meteor’, ‘Meteor’ × ‘Mahalli’, ‘Meteor’ × ‘Majarestani’, ‘Meteor’ × ‘Genotype 1’, ‘Mont Morency’ × ‘Mahalli’, ‘Mont Morency’ × ‘Majarestani’, ‘Mont Morency’ × ‘Genotype 1’, ‘Mahalli’ × ‘Majarestani’, ‘Mahalli’ × ‘Genotype 1’ and ‘Majarestani’ × ‘Genotype 1’, respectively. For each cross, 2 branches were randomly selected in female cultivars in winter. On each branch, including 30 floral buds at ‘balloon stage’ were tagged and bagged to prevent the entrance of any foreign pollen. On the day of anthesis, ready-to-dehisce anthers were collected from male cultivar, maintained on a moist filter paper in petri plates at 4 ºC in refrigerator till they dehisce. Pollens from these anthers were used as a source of male gametes later. Once stigma turned receptive, cross-pollination treatments were carried out in the field as designed scheme for 12 successive days. After every 24 hrs. up to 120 hrs. post-pollination, 12 pistils per treatment were fixed in FAA (formaldehyde/glacial acetic acid/70% alcohol in the ratio of 1:1:18) for 24 hrs. Followed by washings with water to remove traces of alcohol, if any, and cleared in 5% KOH at 60 ºC for fluorescence microscopy. Staining regime, as described in Sabbaghpour et al. (2020) the number of pollen tubes in the upper and mid-parts of the style and in the ovary was employed to enable visualization of the pollen germination on the stigma. Percentage of pollen germination was determined as the proportion of pollen grains that germinated on the stigma to the total number present on a scale of 100. Pollen tube number at three levels was calculated as an average of 5 (n=10). For an easy understanding of growth pattern of pollen tubes inside the style and ovary, the stylar portion, which measures 1.8 ± 0.07 cm on an average, was divided into two parts: the middle and the base. A single, longitudinal incision was then carefully given on one portion of the style using a fine needle and made wide open. Pollen tubes inside the style were critically observed under fluorescence microscope at 100x magnification. Number of them was carefully recorded and data generated for different experimental sets. Results and Discussion: Results showed that maximum pollen tube number penetrated to the ovary and final fruit set were observed in cross ‘Mahalli’בMajarestani’. Also, minimum pollen tube in the ovary and final fruit set were observed in ‘Meteor’בMahalli’ and ‘Meteor’בGenotype 1’ crosses. Conclusion: Based on the obtained results cross-incompatibility was not observed among the studied cultivars and genotypes. Therefore, they could be planted in the sour cherry breeding programs and orchards establishment based on their blooming time.
Hassan Akbari
Abstract
In order to determinate the self–incompatibility and the most suitable pollinizer for Esfahan commercial quince cultivar, the effects of four different pollinizer treatments ((KM1, PK2, Kvd2, NB4), as self pollination and open pollination) on fruit set and quantitative and qualitative characteristics ...
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In order to determinate the self–incompatibility and the most suitable pollinizer for Esfahan commercial quince cultivar, the effects of four different pollinizer treatments ((KM1, PK2, Kvd2, NB4), as self pollination and open pollination) on fruit set and quantitative and qualitative characteristics of commercial quince fruits of Isfahan were studied in a randomized complete block design with 4 replications in quince orchards of Felavarjan region during 3 years. The results of fruit set percentage showed that the mean comparison test had a significant difference between different pollination treatments, except (KVD2, NB4). The maximum fruit set percentage was 23.42 which were related to KM1 genotype, and the minimum percentage was 8.4 which were related to the self pollinated treatment. The mean comparison of self pollinated treatments on fruit weight had a significant difference between KM1 and other treatments. The investigation of quality and quantity characteristics of fruit results showed that there was a significant difference between the Total Soluble Solids (TSS) and firmness. The KM1 genotype (Isfahan sour quince) with 80% of overlap flowering period with 23.42 % of fruit set during three years is chosen and introduced as the best pollinizer for the Isfahan quince cultivar.
