Ornamental plants
Sajjad Heidari; Saeid Reezi; Seyyed Najmodin Mortazavi; Ali Nikbakht
Abstract
Introduction: Lily (Lilium spp.) is one of the most beautiful and popular ornamental bulbous plants that belongs to the Liliaceae family. Lilies (Lilium spp.) belong to one of the six more important genera of bulbous flowers, which is the fourth most famous plant in the world after Rose, Dianthus, and ...
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Introduction: Lily (Lilium spp.) is one of the most beautiful and popular ornamental bulbous plants that belongs to the Liliaceae family. Lilies (Lilium spp.) belong to one of the six more important genera of bulbous flowers, which is the fourth most famous plant in the world after Rose, Dianthus, and Chrysanthemum. The genus Lilium comprises more than 100 species, which are mainly distributed in the northern hemisphere. These species are taxonomically divided into seven sections, including Martagon, Pseudolirium, Lilium, Archelirion, Sinomartagon, Leucolirion, and Oxypetalum. It has been suggested that early removal of Lily buds should enhance Lily bulb yield. Disbudding improves the process of bulb development through its influence on the accumulation and transport of carbohydrates in Lily bulbs. In several studies, the effect of bud removal on vegetative characteristics and propagation of Lily bulbs including cut flower length, leaf area, size and weight of the bulb, number, and size of bulbs, number, and size of scales have been reported. With considering the importance of Lily bulb production in the country and the lack of current research on new Lilium hybrids, including various Oriental and OT hybrids, this study was conducted to investigate the effect of cultivar and disbudding on the vegetative characteristics of Lily flowers and its effect on production and multiplication of Lily. Materials and Methods: To study the effect of cultivar and disbudding on the growth and bulb production of Lily, a factorial experiment in a completely randomized design with two cultivars (the Tiber and Donato), and three disbudding items (D0= No disbudding, D1= Disbudding at the stage of bud appearance, and D3= Disbudding at the stage of 3 cm bud length) was conducted in three replications. This research was carried out in hydroponic greenhouses in Dehaghan city, Isfahan province, in an area with a longitude of 51˚, 61´ E, 31˚, 96´ N, and 2004 m mean above sea level. Temperature, humidity, and light intensity inside the greenhouse during the growing period was 15-25 ℃, 50-70 percent, and 20-30 kilolux, respectively. Evaluated characteristics included the plant height, stem diameter, number of leaves, leaf area, number of buds, bulb weight, number of the scale, scale size, number of bulblets, and propagation coefficient. Results and Discussion: The results showed that cultivar and disbudding (Except stem diameter) significantly affect all evaluated traits, while their interaction had a significant impact on bulb weight, bulblet number, propagation coefficient, bulb size, and scale size. In the Donato cultivar, plant height, stem diameter, leaf area, bulb weight, bulb size, scale size, number of bulbs, and propagation coefficient were 32.9%, 6.9%, 35.3%, 40.9%, 14.2%, 70.1%, 77.9%, and 8.2%, respectively higher than Tiber cultivar, while in the Tiber cultivar, the number of leaves and number of scales was 12.5% and 54.9%, respectively higher than the Donato cultivar. The highest plant height and leaf area were observed in non-disbudding treatment. However, the shortest stem and the smallest leaf were observed in disbudding at the stage of bud appearance and disbudding at the stage of 3 cm bud length which was 10.1% and 9.4%, respectively less than the non-disbudding treatment. By disbudding at the stage of bud appearance, the maximum number of scales, the heaviest bulb, the largest bulb, and the maximum scale size was measured that increased by 11.3%, 91.2%, 23.3%, and 39.2%, respectively compared to the non-disbudding treatment, while the lowest value of mentioned traits was recorded with non-disbudding treatment. By applying bud removal treatments up to the third level, the number of leaves, the number of bulbs, and propagation coefficient increased by 2.7%, 37.0%, and 3.6%, respectively, compared to the non-disbudding treatment, although there was no statistically significant difference between disbudding at the stage of 3 cm and disbudding at the stage of bud appearance. On the other hand, the lowest value of these traits occurred in the non-disbudding treatment. In the Tiber cultivar, the highest bulb weight, bulb size, and scale size were obtained by disbudding at the stage of bud appearance, which showed an increase of 93.5%, 24.0%, and 53.9%, respectively, compared to the non-disbudding treatment. Furthermore, in the Donato cultivar, disbudding at the stage of bud appearance significantly increased bulb weight, bulb size, and scale size by 89.4%, 22.8%, and 31.5%, respectively, compared to the non-disbudding. Also, the highest bulblet number and propagation coefficient in this cultivar were obtained by disbudding at the stage of 3 cm bud length, which increased 58.3% and 0.7%, respectively compared to the non-disbudding treatment. Conclusion: In general, it can be concluded that the Donato cultivar has a significant advantage in most of the evaluated traits over the Tiber cultivar, which can be attributed to the genetic distance between different Oriental and OT hybrids. Disbudding at the stage of bud appearance, if the end of the branch is not damaged, was the most appropriate treatment for replanting, production, and propagating of Lily bulb.
Fereshteh Kamiab; Hamid Mohammadi
Abstract
Introduction: Narcissus is one of the most important ornamental plants in the world. The deficiency of microelements is a major problem limiting the production of ornamental plants under high-pH soils in most regions of Iran. Cu and Mn are essential microelements for the growth and development of plants ...
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Introduction: Narcissus is one of the most important ornamental plants in the world. The deficiency of microelements is a major problem limiting the production of ornamental plants under high-pH soils in most regions of Iran. Cu and Mn are essential microelements for the growth and development of plants as they are involved in many physiological reactions. Fe is involved in the synthesis of chlorophyll and many enzymes, respiration, and nucleic acid metabolism. Cu is a cofactor for numerous enzymes and is also involved in ethylene biosynthesis and activity, respiration, photosynthesis, regulatory proteins, cell membrane metabolism, and hormone signals. The deficiency of microelements, especially Fe, influences productive and reproductive growth of ornamental plants. Thus, this experiment aimed to evaluate the uptake rate of Cu and Mn nano-chelates and their effects on some morphological and physiological Characteristics of ‘jonquil’ narcissus.
Materials and Methods: This study was carried out in the research garden of Agriculture Faculty of Rafsanjan University in 2015 in factorial experiment based on randomized complete block design with two factors of Fe at four levels of 0, 1, 2 and 3 (g/l) and Cu at three levels of 0, 1 and 2 (g/l) in three replications. The weight of the cultivated bulbs were about 8-10 g. They were planted in eight rows spaced 20 cm with on-row spacing of five cm at the depth of 15 cm in each plot in August. According to soil analysis, 40 tons of manure and 100 tons of urea per ha were applied. The nano fertilizer was purchased from Nano-research Biozar Company. Foliar application was done three times: 1- when the plants had three leaves, 2- before flowering and 3- after flowering at 45-day intervals. Different parameters were recorded including plant height, number of leaves, width and length of leaves , number of main bulb, main bulb weight and diameter, , bulblet diameter and weight, flowering stem height and diameter, number of florets, florets diameter, fresh and dry weight of florets, flower vase life, Cu and Fe concentration, protein, chlorophyll and total soluble solids. Foliar spraying was done three times at the intervals of 45 days.
Results and Discussion: The results showed that the concentration of Cu and Fe in the leaves were enhanced with increase in the rate of each nano-chelate, implying high uptake rate of these nano-chelate by leaves in this plant. The application of Fe (2g/l) and Cu (0.5 g/l) resulted in the highest vegetative growth such as plant height, number of leaves, leaf width and length, number of main bulb, main bulb weight and diameter , and bulblet diameter and weight. High pH in experimental soil caused low absorption of micro elements in this condition. This deficiency was compensated with foliar application of nano-chelates and as a result, the Fe and Cu involved processes were improved in them. On the other hand, enhanced Fe and Cu concentrations in leaves could cause more photosynthesis and higher level of assimilation in plants. Likewise, these elements influence some enzymatic activity such as peroxidase, catalase and cytochrome oxidase; consequently, more vegetative growth was observed. Reproductive growth such as flowering stem height and diameter, number of florets, floret diameter and fresh and dry weight and flower vase life were improved in this experiment. The control of abiotic stress is one of the most important roles of Cu and also it is cofactor of superoxide dismutase that has been known as a free radical scavenger in plants that could increase quantitative and qualitative traits of flower. This treatment resulted in the highest amount of protein, chlorophyll and total soluble solids in the leaves of narcissus. Fe is one of the essential elements for chlorophyll synthesis. It also has an important role in activating nitrate reducates enzyme that uses ammonium to synthesize proteins. Cu is involved in the synthesis of proteins and enzymes that have major roles in such processes as respiration, photosynthesis, phenol metabolism, lignifications and ouxin regulation in plants. Thus, the results of this experiment showed that these nano-chelates were absorbed considerably by leaves of narcissus and they are suitable for foliar application. It should be noted that higher concentration of this fertilizer in this experiment has toxic effects. It is suggested that 2g/l Fe plus 0.5 g/l Cu were used to improve vegetative and reproductive growth of ‘jonquil’ narcissus, especially in high-pH soils.
Esmaeil Chamani; Zahra Eftekhari; Alireza Ghanbari; Hamid Reza Heydari; Mousa Arshad
Abstract
Introduction: Fritillaria imperialis L. is an ornamental and medicinal plant native to mountainous regions of Iran. This plant genetic resource is in danger of extinction, Because of grazing livestock and pest outbreaks. Therefore, micro propagation of Fritillaria through in vitro regeneration is essential ...
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Introduction: Fritillaria imperialis L. is an ornamental and medicinal plant native to mountainous regions of Iran. This plant genetic resource is in danger of extinction, Because of grazing livestock and pest outbreaks. Therefore, micro propagation of Fritillaria through in vitro regeneration is essential for conservation and commercial production. Thymol and Carvacrol are one of the main essential oil compounds in family Lamiaceae.
Material and Methods: Fritillariaimperialis L. bulbs in dormancy stage obtained from mountainous regions of Lorestan in Iran and were placed in cold room at +4 °C for 4-6 weeks. Then, Bulbs were surface-sterilized with 70% ethanol for 45 s followed by immersion in 5% (v/v) NaOCl solution for 20 min with gentle agitation, and then rinsed three times in sterile double distilled water. Present study was conducted in two separate experiments. In first experiment, effect of different concentration of Thymol and Carvacrol and in second experiment, different concentration of NAA and BA on in vitro characteristics of Fritillaria was evaluated. Explants (1× 1 cm) prepared from the lower third of scales with basal plate and were placed in MS basal medium supplemented with different concentrations of Thymol (50, 100, 150 and 300 ppm), Carvacrol (10, 100, 500 and 100 ppm), BA (1, 2 and 4 mg/l) and NAA (1, 2 and 4 mg/l).All cultures were incubated in a growth chamber at 24±2°C, and a photosynthetic photon flux of 40-60 μmol m–2 s–1 was provided by cool white fluorescent lamps with a 16-h photoperiod. This experiment wascarried out in completely randomized designs with fivereplications.
Results and Discussion: Analysis of variance showed that Thymol and Carvacrol were not effective on number of new bulblets but had significant effects on bulb diameter, number and length or roots, number and length leaves and callus induction and diameter of callus obtained from scales (P< 0.05). The highest rate (3 bulblets) of bulblets formation was obtained fromMS medium supplemented with 50 ppm Thymol that showed significantly difference from other treatments. Medium containing 10 ppm Carvacrol gave the highest Bulblet formation (2.5 bulblets) between Carvacrol treatments. Investigation of rooting was done by assessment of the number and length of roots. Mean comparison of the effect of cultivar type on root number showed that the largest number of roots per explant was obtained fromMS medium containing 50 ppm Thymol. Lowest number of roots observed in mediums supplemented with 300 ppm Thymol and 100 ppm Carvacrol. The best medium for increasing the root length per explant (10.90 cm) was MS medium supplemented with 100 ppm Carvacrol, while the least increasing in root length per explant observed from culture mediums contained 300 ppm Thymol and 100 ppm Carvacrol. Also, the largest number of leave formation obtained from culture medium supplemented with 50 ppm Thymol that significantly higher than other treatments. Statistical analysis (ANOVA) of the data showed that high frequency callus induction and formation occurred in MS mediums contained 50, 100 and 150 ppm Thymol and 10 ppm Carvacrol and culture mediums supplemented with 300 ppm Thymol and 1000 ppm Carvacrol showed least callus induction. In contrast, largest callus diameter observed in culture mediums supplemented with 300 ppm Thymol and 500, 100 ppm Carvacrol.
Statistical analysis of results showed that different concentrations of BA and NAA had significant effects on bulblets number and bulblets diameter (P
Nasrin Farhadi; Saeideh Alizadeh Salteh
Abstract
Introduction: Allium hirtifolium commonly known as Persian shallot is an important wild medicinal plant from Alliaceae family. Persian shallot commonly known as mooseer in Iran is a perennial diploid plant that is native to Iran and grows as a wild plant throughout in the Zagross Mountains range, western ...
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Introduction: Allium hirtifolium commonly known as Persian shallot is an important wild medicinal plant from Alliaceae family. Persian shallot commonly known as mooseer in Iran is a perennial diploid plant that is native to Iran and grows as a wild plant throughout in the Zagross Mountains range, western and southwestern Iran. It is a bulbous herb and usually consists of a single main bulb or rarely two bulbs. Each bulb has a weight of about 8-15 times of a garlic clove. The bulbs of mooseer has been widely used as a traditional herb and spice plant, added to a variety of foods such as salads, pickles, yogurt and different sauces. Conventionally, Persian shallot propagates through bulbs and seeds but these two methods are not commercially efficient due to low growth rate of bulbs and deep dormancy, low viability and germination rate of seeds. In addition, the natural habitat of this plant is under increasing pressure as a result of excessive incorrect harvest that caused to damage the plant density in Iran rangelands. So, improving the efficiency of A. hirtifolium propagation is necessary. A number of positive effects on the growth and productivity of some plants through cytokinin application have been registered by earlier research. The current study aimed to evaluate the effects of pretreatment and foliar application of forchlorfenuron as a safe cytokinin on improving the bulb production, phytochemical compounds and antioxidant attributes of Persian shallot.
Materials and Methods: This experiment was done at research green house of Tabriz University in 2015-2016. For pretreated of Persian shallot bulbs, they were soaked in 0, 50 and 10 mg l-1 forchlorfenuron solutions for 24 h. Then they were cultured in pots contained perlite and vermicompost with 3:1 ratio. Foliar application was applied 2, 4 and 6 weeks after culture with 0, 50, 100 and 150 mg l-1 concentrations of forchlorfenuron. At the end of growth season the number of leaves, number of bublets, fresh and dry weight of bulblet were recorded. The phytochemical compound (protein, phenol and allicin), antioxidant enzymes (catalase, peroxidase, ascorbate peroxidases and superoxide dismutase) and antioxidant activity of bulbs were assayed with spectrophotometry methods.
Results and Discussion: Foliar applications of plant growth regulators such as cytokinins in agriculture crops are reported to be useful in controlling multiple physiological processes, including flower initiation, shoot elongation, bulb production, fruit set and as well as affected the quality characters of products. In this study despite the bulblets number that did not influence by treatments, pretreatment and foliar application of forchlorfenuron significantly increased the leaves number, fresh and dry weight of bulbs in comparison with control plants. The highest leaves number (4.49 per plant) was obtained from pretreatments. The highest fresh weight (91.77 g) was recorded at 5 and 10 mg l-1 pretreatment and 100 mg l-1 (91.63 g) foliar application. The interaction effect of treatments on dry weight was significant and the highest dry weight (19.75 g) was recorded at 10 mg l-1 pretreatment with 100 and 150 mg l-1 foliar application. Allicin content did not show significant variation between treatments and in average was 0.859 mg g-1 FW. Total phenol content significantly influenced by treatments and the highest phenol content (1.585 mg GAE g-1 FW) was recorded at 5 mg l-1 pretreatment with 100 mg l-1 foliar application. The antioxidant enzymes included catalase, peroxidase and ascorbate peroxidase that showed significant increasing under forchlorfenuron treatments. Due to significant effects of forchlorfenuron on antioxidant compounds and enzymes of Persian shallot bulbs, the assay of antioxidant activity also showed a significant increasing in treated bulbs. The maximum percent of antioxidant activity (74.522) was obtained from 100 mg l-1 foliar application. Exogenous application of cytokinins plays an effective role by protecting the fluidity and integrity of plant cell membranes. They properly mediate enzymatic (SOD, APX, and CAT) and non-enzymatic machinery with the result of preventing cell membrane damage by oxidative stress.
Conclusions: Considerable improvement in biochemical and antioxidant attributes of Persian shallot was recorded with pretreatment and foliar application of forchlorfenuron. The present data support the potential uses of the forchlorfenuron for improving the production of weighty bulbs with the high antioxidants attributes in Allium hirtifolium. Pretreated and foliar application at 5 mg l-1 and 100 mg l-1 concentrations of forchlorfenuron, respectively showed the best results and is recommendable for A. hirtifolim production.
