Farnoosh Malekshahi; Ali Ashraf Mehrabi; Elahe Tavakol; Khosro Mehdikhanlo; Vahid Shariati
Abstract
Introduction: Basil genus (Ocimum) contains 30 to 150 species which grown in tropical and subtropical regions of Asia, Africa, Central and South America and found as a wild plant in these areas. In India, around 25,000 ha is under cultivation of Ocimum spp., with an annual production of about 250–300 ...
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Introduction: Basil genus (Ocimum) contains 30 to 150 species which grown in tropical and subtropical regions of Asia, Africa, Central and South America and found as a wild plant in these areas. In India, around 25,000 ha is under cultivation of Ocimum spp., with an annual production of about 250–300 tonnes of essential oil. Ocimum gratissimum L., a dicotyledonous shrub plant, which belongs to the Lamiaceae family, stands out for the quality, quantity and chemical diversity of the essential oils. These oils have been used in the pharmaceutical, cosmetic and food industries. Some of the essential oil compounds have antibacterial, insecticidal and antioxidant properties with high demand on the international market of the fine perfumery industry. It is also popularly used in herbal medicine for treating several diseases, such as upper respiratory tract infection, fever, cough, diarrhea and pneumonia. Being a short-duration economically viable medicinal and aromatic crop, clove basil has huge potential for large scale cultivation. Plant genetic has an important role in determining the type and amount of secondary metabolites of medicinal plants. Moreover, the recognition of species and genotypes with high genetic capability in the production of desired metabolites has been at the top of the plant breeding plans of medicinal plants. In addition, essential oil composition of plants may be affected by harvest time which is due to the impact of weather conditions on plant growth and development. The present study was aimed to evaluate the oil composition of two genotypes in two harvests. Materials and Methods: The research was conducted in the research farm of the college of agriculture, shahid Chamran University, Ahvaz, Iran during 2019. Two valuable genotypes of Ocimum gratissimum L. (278 and 296), with two different essential oil profiles, were investigated in two harvests; spring and autumn seasons. The aboveground parts of the plants were collected on June and November and dried on shade at room temperature. The essential oils of the plants were extracted by water distillation through Clevenger apparatus and the quantity and quality of the essential oils were analyzed by GC and GC-MS. Results and Discussion: The results of present study showed that the essential oil content of two genotypes was not affected by the harvest season while its amount was different in two genotypes. The essential oil content of genotype 296 was 2-fold of 278. According to the qualitative analysis of the essential oils, fifty compounds were identified in the essential oils of 278 and 296 genotypes. More than 98% of the identified compounds (in the essential oils of these two genotypes) were classified into five chemical classes; including hydrocarbon and oxygenated monoterpens, and hydrocarbon and oxygenated sesquiterpene and phenylpropanoids. The major constituent of the essential oil of genotype 278 was oxygenated monoterpene, thymol, on June (35.48 %) and November (45.85 %), which was not found in genotype 296. Gamma-terpinene was also significantly increased from June (13.15 %) to November (25.80 %). P-cymene (11.31-3.56 %), alpha- thujone (4.76-2.94 %), Germacrene D (3.73-2.76 %), caryophyllene E (3.66-1.51 %), myrcene (2.93-3.01 %), alpha-terpinene (2.63-3.38 %) and bourneol (2.28-0.71 %) were the remains of oil composition. Dihydro eugenol, which belongs to the chemical class of phenylpropanoids, was identified as the main essential oil components of genotype 296 which its amount was not affected by the harvest time. The other oil constituents were Beta (Z)-Ocimene (11.89-3.40 %), Germacrene D (3.58-2.80 %), and caryophyllene E (0.52-2.68 %). Conclusion: Terpenoids such as thymol are synthesized via the mevalonic acid pathway, and phenylpropanoid compounds such as dihydroeugenol and eugenol are synthesized via the shikimic acid pathway. The metabolite diversity across different species could be explained by the differential gene expression pattern. According to the results of the present study, thymol was identified as the main oil components of genotype 278. This may be due to the increased expression of mevalonate enzymes. The monoterpene was replaced by phenylpropanoid; dihydrogenugenol, in the oil of genotype 296 which might be due to more expression of the enzymes of the phenylpropanoid pathway. In the other hand, Thymol, P-cymene and gamma-terpinene in genotype 278 varied significantly in different harvesting times, indicating the effect of temperature on the activity of enzymes involved in the synthesis of essential oil components. On the contrary, the amount of dihydrogenugenol in genotype 278 on June and November is not affected by the environmental conditions in two seasons. With regard to the conclusions to the proper growth of genotype 278 and 296, as well several harvests annually, essential oil content and thymol and dihydrogenugenol, therefore, it is suggested that further research should be carried out for developing plant cultivation in Khuzestan and southern provinces which is not suitable for basil growth.
Seyyed Mehran Alavi; Asad Masoumiasl; Naser Zare; Rasul Asghari Zakaria; Parisa Sheikhzade Mosaddegh
Abstract
Introduction: The main habitat of Chavil, Ferulago angulata, in Iran is Zagros area. This plant has a rejuvenating effect and is used to treat digestive diseases and intestinal worms. Because the different explants show different amounts of callogenesis under the effect of different growth regulators, ...
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Introduction: The main habitat of Chavil, Ferulago angulata, in Iran is Zagros area. This plant has a rejuvenating effect and is used to treat digestive diseases and intestinal worms. Because the different explants show different amounts of callogenesis under the effect of different growth regulators, selection of an optimal explant and suitable plant growth regulators combination has a significant effect on the production of callus and their suspension culture. There is no reports on Ferulago angulata callogenesis and its cell suspension culture. Therefore, this study was designed and implemented to optimize callus production and cell suspension culture in this important medicinal plant.
Materials and Methods: Seeds of Chavil were collected from four different habitats in Kohgilooyeh and BoyerAhmad Province in Southwest of Iran include Abenahr, Guayoune, Vezg and Sisakhat. Seedlings obtained from embryo culture were used to prepare the explants. Various explants (leaf, root and stem) were cultured on MS medium supplemented with different concentrations (0, 0.5, 1 and 2 mgl-1) of NAA and BAP. Callus traits were evaluated and from the best culture medium, the best explants and the best PGRs composition for callogenesis of each ecotype were used to cell suspension culture. In order to study the growth rate of cells in suspension culture and plotting the curve of cells growth, two cell density indices and packed cell volume index were evaluated. To determine the cell density index, every 3 days, 10 ml of cell suspension were transferred to the graded falcon and centrifuged at 5000 g for 5 minutes, and the percentage of sediment cells was calculated as the total volume. To determine the packed cell volume index, also every 3 days, 10 ml of culture medium containing cells were transferred to the graded falcon and stored for 30 minutes to precipitate cells and cell masses. Finally, the cell volume was recorded and was calculated as percentage of the total collected medium.
Results and Discussion: According to the callogenesis percentage, the best ecotype is Abenahr and best explant is leaf explant. The highest level of NAA is 2 mgl-1, and the best level of BAP is 2 mgl-1, which causes 100 callogenesis percentage. The best medium for cell suspension culture is MS medium containing 2 mgl-1 NAA and 0.5 mgl-1 BAP for callus was obtained from leaf explant of Abenahr ecotype. Along with these plant growth regulators, 2,4-D was used in combination with BAP to form suspension culture. The results also showed that 2 mgl-1 2,4-D plus 0.5 mgl-1 BAP were useful in producing suspensions. The difference between 2,4-D +BAP and NAA + BAP combinations more cell volumes were observed, and cell suspension was created at a faster rate and in less time, which is an advantage in research work. Growth rate of cell suspension originated from the leaf explant was higher than root explant. In terms of culturing cell suspension, the Abenahr ecotype was favorable compared to other ecotypes. During cell suppression culture of Cyperus aromaticus by applying different levels of NAA, cell growth was increased up to 3 weeks after application, and then decreased. By applying 2,4-D, cell growth also increased until the third week, and after the third week, cell growth declined, which was very low growth rate compared with the NAA. In cell suspension culture of sugar beet, using 2,4-D was much more effective than NAA on all explants. In the present study, 2,4-D was also more effective than NAA for cell suspension culture of Chavil.
Conclusion: In general, the Abenahr was the best ecotype among of investigated. The explants in both callus culture and the suspension culture, and the best combination of plant growth regulator in both culture was 2 mgl-1 NAA plus 0.5 mgl-1 BAP.
Asad Masoumiasl; Ameneh Aryiaeineghad; Masud Dehdeari
Abstract
Introduction: German (Matricaria chamomilla L.) and Shirazi (Matricaria recutita L.) chamomiles are the most important medicinal plants of the Astraceae family which are used in the pharmaceutical, health, food and cosmetics industries. Production of this plant has been undertaken in Iran mainly in Isfahan, ...
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Introduction: German (Matricaria chamomilla L.) and Shirazi (Matricaria recutita L.) chamomiles are the most important medicinal plants of the Astraceae family which are used in the pharmaceutical, health, food and cosmetics industries. Production of this plant has been undertaken in Iran mainly in Isfahan, Kohgiluyeh and Boyerahmad, Golestan and Hamedan provinces. In vitro propagation of plants have higher potential to produce qualified natural products, restoring and preserving of endangered plants, induction of somaclonal variation, industrial reproduction, valuable secondary metabolites and increased active ingredients. Researchers reported successful micropropagation system for five chamomile varieties on MS medium contained 0.01 mg/l NAA and 2.5 mg/l kinetin. The aim of this study was to investigate the effects of stem (with and without node), leaf and cotyledon explants and different plant growth regulators on direct regeneration of German and Shirazi chamomiles. Although the Shirazi chamomile is native to Iran, but German chamomile is Iran non-indigenous cultivar. By our knowledge, there have been no comparison reports about responses of these cultivars to tissue culture.
Materials and Methods: This research was performed in the central laboratory of Agriculture Faculty in Yasouj University. Seeds were provided from Pakan-Bazr institute, Isfahan. Chamomile seeds were disinfected by ethanol (70%) for 5-10 min and sodium hypocholorite 3% for 5-12 min and then washed for several times by distilled water. Then, seeds were sown on MS medium for germination. After 2-3 weeks, seedlings were grown and then planted in MS medium supplemented with hormonal combinations of NAA at two levels (0.1 and 0.5 mg/l), kinetin at three levels (2, 2.5 and 3 mg/l) and Zeatin, BAP and 2ip at three levels (0.5, 1 and 1.5 mg/l). The experiment was performed in a completely randomized design with four replications. Factors included explants, cultivars and hormonal combinations. In this experiment, traits such as stem induction percentage, stem length, stem fresh and dry weight root induction percent, root length and stem fresh and dry weight were measured. Statistical analysis was performed using SAS software (version 9.1). In order to test the normality and perform mean comparisons, Minitab 14 and MSTAT-C software was used and excel software was used for drawing diagrams.
Results and Discussion: For all traits except stem induction percent and root dry weight, triple interaction of explants, hormonal combinations and cultivar were significant at 1% level. Triple mean comparisons for cultivar, explant and hormonal combinations shows that the highest mean for stem height, stem fresh weight, stem dry weight, root induction percent, root height and root fresh weight was obtained in Shirazi Chamomile cultivar with cotyledon explants in hormonal combinations of 0.1 mg/l NAA and 1.5 mg/l 2ip. The results showed that the best explants in both chamomiles for direct regeneration were stem (whit node) and cotyledon with 78.75% and 75% regenerations, respectively. Also the best genotype and hormone combination were Shirazi chamomile and MS medium supplemented with 1.5mg/l 2ip with 0.1mg/l NAA. To determine the best hormonal combination for root regeneration from direct regeneration, the stems were embedded in medium contained different concentrations of IBA. The first signs of rooting production were observed after 5-7 days. After completing the roots formation (4 weeks after transferring), the rooting percentage, root length, fresh and dry weight of roots were measured. Based upon variance analysis, effect of triple interactions of IBA, explants and cultivar on all traits were not significant, but the effect of IBA for all traits was significant at 1% level. According to the results of mean comparisons for effects of IBA on root traits in direct regeneration of chamomile, the highest percentage of root regeneration (73.75 %), the highest root length (6.60 cm), root fresh weight (174.167 mg) and the highest root dry weight (16.425 mg) were obtained from medium contained 0.5 mg/l IBA. Root differentiation was influenced by auxin (0.5 mg/l IBA) or spontaneously. Regenerated plantlets were transferred to pots contained sterilized soil (3:1:1 mixture of soil: sand: leaf compost). For plant adaptation to natural conditions, glassy caps were used. After adaptation, the caps were removed and the plants were transferred into a growth chamber. Previous studies reported that MS medium supplemented with 0.2 to 1 mg/l of BA and 2 mg/l of NAA induced adventitious bud formation and shoot development in leaf explants of Roman Chamomile. A higher number of adventitious buds were observed at the proximal end of the explants. Plantlets were rooted on MS medium supplemented with 0.1 mg/l of IBA and successfully weaned in vivo.
Conclusion: Based on the results of this research, chamomile showed relevant response to direct regeneration.
Rasul Najib; Mohammad Farsi; Amin Mirshamsi kakhki; Saeid Reza Vessal
Abstract
Introduction Tomato (Lycopersicon esculentum Mill) is one of the most important vegetables which in addition of its importance as a food, is utilized as a model plant for cytological and cytogenetic studies. Tomato breeding programs are often based on the production and selection of hybrid plants. Producing ...
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Introduction Tomato (Lycopersicon esculentum Mill) is one of the most important vegetables which in addition of its importance as a food, is utilized as a model plant for cytological and cytogenetic studies. Tomato breeding programs are often based on the production and selection of hybrid plants. Producing hybrid plants and application of features that is needed to breed pure lines with high specific combining abilities, is highly required.New technologies such as doubled haploid can be an effective strategy to provide pure lines in tomato. Generation of homozygous doubled haploid lines through induction of androgenesis is a promising alternative method to the classical breeding programs. However, this technology is poorly developed in tomato so that some improvements in methodology are required. Genotype and stages of microspore development are critical factors for induction of androgenesis in tomato. Among them, the genotype is more important than other factors. The purpose of this study was to investigate the possibility of callus induction from anthers in some tomato genotypes.
Materials and Methods: In order to investigate the androgenic response and callus induction through anther culture in tomato, four varieties including Mobil-Netherlands, Baker, U. S. Agriseed and Khoram were chosen. To determine the appropriate stage of microspore development for anther culture, cytologycal studies were accomplished at different size length of flower buds (2-7.9 mm). Flower buds were incubated at 4oC for 15 minutes and stained in acetocarmin %4 solution. Based on cytological studies in four tested cultivars, flower buds with size length 4-4.9 mm were chosen, as they had the highest frequency of meiotic microspores to microspores mid uninucleate. Pretreatments were colchicine solution (250 mgr/L) at 4 °C for 48 h. The anthers were cultured on MS medium containing 2 mgr/L IAA and 1 mgr/L 2ip. All changes in frequency of callus induction and diameter of callus were recorded for eightweeks. Diameter of callus was measured using a microscope equipped with a camera and Dino Capture 2.0 software version 4.1. Cytological studies were accomplished by microscopy research Olympus BX51 and photographed by a digital camera DP70. To determine the presence or absence of a significant difference between the observed proportions a chi-square test was used. All analysiswas conducted using statistical software JMP 8. Charts were providedusing Excel software.
Results and Discussion: Anther development stage is one of the factors determining the success of anther culture in the production of embryos. The results of most studies showed that the stage between meiosis and mid-stage of unicellular microspores is optimum to androgenesis response in tomatoes. Since microspores in the anthers are at various stages of development, to determine the appropriate size of flower buds, the relative frequency of each of the stages of development should be understood. Based on the obtained results, in all study cultivars, flower buds with a length of 4-4.9 mm (Containing anthers with an approximate length of 3-4 mm), due to having the highest frequency of meiotic and unicellular microspores, can be used for anther culture. Study of deformation and induced callus in this experiment showed that both the Baker and U. S. Agriseeds did not show callus induction. Anthers of varieties over three weeks after culture gradually became yellowish-brown and in the fourth week of the increased frequency of haploid were brown. After six weeks of culture, all anthers in both became brown and died. The anthers of the varieties, Mobil-Netherlands and Khoram, inflated at the second to fourth week, anther wall was eventually broken and callus was observed. At third week the frequency of deformed anthers were gradually increased. Four weeks after culture, the frequency of callus induction reduced and after five weeks of callus induction no change in frequencyof callus induction was observed. The results showed that frequency of callus induction was significantly different among genotypes (P
Hassan Akbari
Abstract
In order to determinate the self–incompatibility and the most suitable pollinizer for Esfahan commercial quince cultivar, the effects of four different pollinizer treatments ((KM1, PK2, Kvd2, NB4), as self pollination and open pollination) on fruit set and quantitative and qualitative characteristics ...
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In order to determinate the self–incompatibility and the most suitable pollinizer for Esfahan commercial quince cultivar, the effects of four different pollinizer treatments ((KM1, PK2, Kvd2, NB4), as self pollination and open pollination) on fruit set and quantitative and qualitative characteristics of commercial quince fruits of Isfahan were studied in a randomized complete block design with 4 replications in quince orchards of Felavarjan region during 3 years. The results of fruit set percentage showed that the mean comparison test had a significant difference between different pollination treatments, except (KVD2, NB4). The maximum fruit set percentage was 23.42 which were related to KM1 genotype, and the minimum percentage was 8.4 which were related to the self pollinated treatment. The mean comparison of self pollinated treatments on fruit weight had a significant difference between KM1 and other treatments. The investigation of quality and quantity characteristics of fruit results showed that there was a significant difference between the Total Soluble Solids (TSS) and firmness. The KM1 genotype (Isfahan sour quince) with 80% of overlap flowering period with 23.42 % of fruit set during three years is chosen and introduced as the best pollinizer for the Isfahan quince cultivar.
Shadi Mohamadi-Nejad; M. Gholami; Mahmood Esna-Ashari
Abstract
This research was conducted to study the factors affecting establishment, growth and shoot proliferation of Persian walnut, genotype Z60. Explant establishment was studied through a factorial experiment based on a completely randomized design with two factors including culture media (DKW and WPM) and ...
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This research was conducted to study the factors affecting establishment, growth and shoot proliferation of Persian walnut, genotype Z60. Explant establishment was studied through a factorial experiment based on a completely randomized design with two factors including culture media (DKW and WPM) and BA concentration (0 (control), 0.5 and 1.0 µM). Single-node explants were prepared from the young branches of Z60 trees in the middle of May. Although the effect of two culture media on the growth characteristics had no significant difference, but mean comparison of the data with this regard showed that DKW medium performed better than WPM. No significant difference was observed between the two BA concentrations, but significant difference was between two concentrations (0.5 and 1.0 µM) with 0 (control). In shoot proliferation experiment, two cytokine hormones (BA and Kinetin) in three concentrations (4.4, 6.6 and 8.8 µM) were studied in DKW medium. The data obtained from this part of experiments were analyzed through unbalanced completely randomized design. The effect of different hormonal treatments and their concentrations on Z60 genotype growth characteristics showed that 8.8 µM BA was more effective in compared to the other two treatments. However, no significant difference was observed between the three concentrations with this respect.
Zahra Nemati; Ali Tehranifar; Mohammad Farsi; Amin Mirshamsi Kakhki; Seyyed Hossein Nemati
Abstract
Pomegranate (Punica granatum L. that one Punicaceae) is one of the important economical and commercial horticultural plants cultivated in arid and semiarid parts of Iran. Also, due to the long history of pomegranate cultivation in Iran, genotypes from different regions with obvious similarities in appearance ...
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Pomegranate (Punica granatum L. that one Punicaceae) is one of the important economical and commercial horticultural plants cultivated in arid and semiarid parts of Iran. Also, due to the long history of pomegranate cultivation in Iran, genotypes from different regions with obvious similarities in appearance but with different names can be observed. Thus, the precise discrimination between .genotypes is essential for effective management for future pomegranate breeding programs. In this study, AFLP markers based on seven primer combinations (EcoRI/Tru1I) were used to evaluate genetic variation and Phylogenic relationship among 31 different .genotypes of native pomegranate belonging to seven of Iran provinces. According to the cluster analysis, a relatively low genetic diversity was observed across the .genotypes studied. Also, the results showed that the clustering of the cultivars didn’t concerne to morphological traits. The derived dendrogram proved that .genotypes are clustered independently from their geographical origin and their denomination. Heterozygosity index, Principal co-ordinates analysis (PCoA), Gst Index and analysis of molecular variance (AMOVA) revealed noticeable similarity among studied populations and observed variation within populations was very low and near zero. The high level of morphological traits diversity in Iranian pomegranate and low level of polymorphism in genome organization of these .genotypes determined by AFLP markers may be related to the somatic propagation and also type of pomegranate pollination.
Roghayyeh Najafzadeh; Kazem Arzani; Alireza Babaei
Abstract
Abstract
Considering to importance and high genetic diversity of Pear (Pyrus communis L.) in Iran, a study was conducted on the effect of genotype on fruit physicochemical properties and qualitative characteristics of six mature European Pear genotypes in Teheran, Iran environmental conditions. In this ...
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Abstract
Considering to importance and high genetic diversity of Pear (Pyrus communis L.) in Iran, a study was conducted on the effect of genotype on fruit physicochemical properties and qualitative characteristics of six mature European Pear genotypes in Teheran, Iran environmental conditions. In this study some physicochemical fruit properties and qualitative characteristics such as: fruit length, width, length to width ratio, fruit pedicel length, fruit color, fruit fresh and dry weight, fruit volume, fruit firmness, total soluble solids (TSS), titratable acidity (TA), pH and sensory evaluate were monitored based on the descriptors of International Plant Genetic Resources Institute (IPGRI). Results showed significant differences within the studied genotypes in the most fruit characters (P ≤0.05). In addition, it was a diversity within the measured characters in fruit length (ranged from 6.99 to 9.82 cm), fruit width (4.69 to 7.17 cm), fruit length to width ratio (1.23 to 1.59), fruit pedicel length (2.2 to 3.73 cm), fruit color (yellow to yellowish green and green to red with L* value 60.47 to 74.08 and H* value 80.06 to 86.95 and C* value 46.16 to 53.43), fruit fresh weight (80.5 to 308.9 g), fruit dry weight (4.52 to 5.36 g), fruit volume (86.33 to 270.7 cm3), fruit firmness (1 to 2.37 Kg/cm2), TSS (14.9 to 17.63 οBrix), TA (0.18 to 0.41 % malic acid), TSS/TA (40.49 to 92.66) and pH (3.95 to 5.33). Results revealed that genotype A95 had better quality and physicochemical properties and qualitative characteristics than other genotypes which can be considered as promising genotype for further evaluations.
