تأثیر هورمون‌های رشد بر باززایی درون شیشه‌ای زنبق مردابی (Iris pseudacorus)

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه محقق اردبیلی

چکیده

به‌منظور انجام این طرح، سه آزمایش در قالب طرح کاملاً تصادفی در آزمایشگاه کشت بافت گروه باغبانی دانشگاه محقق اردبیلی انجام شد. برای باززایی گیاه از بذر، از غلظت‏‏های مختلف NaOH(5، 10، 15و20 مولار) و خراش‏دهی با کاغذ سمباده (خراش‏دهی نرم، خراش‏دهی متوسط و خراش‏دهی سخت) استفاده شد. نتایج حاصل از مقایسه میانگین داده‏‏ها نشان داد که غلظت 20 مولار NaOH و نیز خراش‏دهی سخت با کاغذ سمباده بیشترین میزان جوانه‏زنی را داشتند. دو ماه پس از جوانه‌زنی بذور، هیپوکوتیل حاصل از دانهال‌های بذری طی دو آزمایش جداگانه تیمار شدند که در آزمایش اول غلظت‌های 1، 2 و 4 میلی‏گرم بر لیتر از هورمون‏های اکسین (پیکلرام و 2,4-D) و در آزمایش دوم غلظت‌های 1، 2 و 4 میلی‏گرم بر لیتر سایتوکینین (TDZ و BA) با 4 تکرار برای هر تیمار، در قالب طرح کاملاً تصادفی کشت شدند. نتایج حاصل از تجزیه واریانس داده‏ها نشان داد که بین تیمار‌ها در مقایسه با شاهد تفاوت معنی‏داری در سطح احتمال 1 درصد وجود داشت. نتایج حاصل از مقایسه میانگین داده‏ها در پارامترهای رشد کالوس نشان داد که غلظت 4 میلی‏گرم بر لیتر پیکلرام و 1 میلی‏گرم بر لیتر بیشترین تأثیر را بر القای کالوس در این گیاه داشت. نتایج حاصل از مقایسه میانگین داده‏ها در پارامترهای رشد گیاهچه نشان داد که تیمار BA با غلظت1 میلی‏گرم بر لیتر بیشترین میزان شاخه‌زایی را در این گیاه تولید نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation on the Hormone Effects on in vitro culture of Iris pseudacorus

نویسندگان [English]

  • E. Chamani
  • M. Taheri
Mohaghegh Ardabili University
چکیده [English]

Three experiments were conducted in tissue culture and biotechnology laboratory of Horticulture Department of Mohaghegh Ardabili University in 2012. For the regeneration of plant from seed, different concentrations of NaOH (5, 10, 15, 20 M) and various scarification methods with sandpaper (soft scarification, medium scarification and hard scarification) were used based on completely randomized design with 4 replications. The results of experiment revealed that seeds treated by 20 M NaOH and hard scarification produced the highest germination rate. After 2 months of seed germination, hypocotyles of seeds were used as explants and cultured in MS medium containing different concentration of 2,4-D, picloram, TDZ and BA (1, 2, 4 mg/l) based on completely randomized design with 4 replications. Mean comparison revealed that explants treated by 4 mg/l picloram and 1 mg/l 2,4-D produced the highest callus content. Mean comparison showed that explant treated by 1 mg/l BA produced the highest shoots. However, to investigate the soluble protein changes during growth stages and to study the effects of 2,4-D, picloram, TDZ and BA on soluble protein and experiment was conducted. The result showed that by increasing the plant age, soluble protein was reduced and also the highest soluble protein was found after 4 weeks of germination. The result also showed that explants treated by 4 mg/l picloram and 1 mg/l BA produced the highest soluble protein content.

کلیدواژه‌ها [English]

  • Micropropagation
  • Scarification
  • Tissue culture
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