ارزیابی اثر بیوفسفر و اسیدهیومیک بر برخی خصوصیات رشدی و میزان روغن بذر گل مغربی

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه فردوسی مشهد

چکیده

به منظور بررسی اثر بیوفسفر و سطوح مختلف اسید هیومیک بر برخی خصوصیات کمی و کیفی گل مغربیآزمایشیبه صورت فاکتوریلدر قالب طرح بلوک های کامل تصادفی با 8 تیمار و 3 تکراردر مزرعه آموزشی دانشگاه فردوسی مشهد انجام شد. تیمارهای آزمایش شامل 4 سطح اسید هیومیک (صفر، 1، 3 و 5 میلی‌لیتر در لیتر) و دو سطح بیوفسفر (کاربرد و عدم کاربرد) بودند. نتایج بدست آمده نشانداد بیشترین ارتفاع گیاه (59/85 سانتیمتر)، تعداد بذر کپسول های ساقه اصلی (17/251) و درصد روغن (75/21درصد) در اثر متقابل بین سطح 5 میلی‌لیتر بر لیتر و بدون کاربرد بیوفسفر و عملکرد بذر (1080کیلوگرم در هکتار) و عملکرد روغن (227 کیلوگرم در هکتار) در اثر متقابل بین سطح 3 میلی‌لیتر بر لیتر و بدون کاربرد بیوفسفر به دست آمد. اثر ساده اسید هیومیک نیز بر بهبود برخی از صفات گل مغربی اثر معنی داری داشت، به طوری‌که بیشترین میزان تعداد شاخه جانبی (8/26) و تعداد کپسول ساقه اصلی (35/117) در سطح 5 میلی‌لیتر بر لیتر با تیمار اسید هیومیک به دست آمد. بیوفسفر نیز بر افزایش تعداد شاخه جانبی تاثیر معنی داری داشت. استفاده از این کود به تنهایی و بدون کاربرد اسید هیومیک بالاترین میزان کپسول شاخه های جانبی را تولید کرد. کاربرد بیوفسفر بر درصد روغن افزایش معنی داری نداشت و عملکرد روغن با کاربرد این کود در سطح صفر و 1 اسیدهیومیک نسبت به شاهد کاهش یافت و در سطح 3 و 5 میلی‌لیتر در لیتر اسید هیومیک تفاوت معنی داری نسبت به شاهد نداشت. از میان سطوح مختلف اسید هیومیک سطح 3 و 5 میلی‌لیتر در لیتر نتایج بهتری بر روی صفات اندازه گیری شده داشتند و به جز درصد و عملکرد روغن در اثر متقابل با بیوفسفر دارای اثرات بهتری بودند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Bio-phosphor and HumicAcid on Growth Parameters and Oil Content in Evening Primrose (Oenotherabiennis L.)

نویسندگان [English]

  • Zeinab Aghakhani
  • Majid Azizi
  • Hossein Aroiee
Ferdowsi University of mashhad
چکیده [English]

Introduction: Evening primrose (OenotherabiennisL.) is a relatively new and valuable oilseed crop for temperate region. Its oilseed is important because of high levels of unsaturated fatty acids, especially gamma-linolenic acid. Seeds contain 20-30% oil which includes 70% linoleic acid (LA) and 10% gamma linolenic acid (GLA). Oil content in O. biennis is a quantitative trait which controlled by many genes and similar to any other quantitative traits varies with environmental conditionssuch factors as age of seed and growth conditions. Evening primrose oil, especially gamma-linolenic acid, has many therapeutic properties, and it uses as cure for many diseases such as Diabetes, Eczema, inflammation, Cardiovascular, Cancer, Autoimmune diseases, Rheumatoid Arthritis, Depression. Studies on Medicinal Plants in nature and farmlands show that the use of sustainable agriculturae andnatural sources are two suitable methods for producing healthy plants.Additionally, the use of bio-fertilizers lead to maximum yield and quality in these plants. Azizi et al.(6) showed that interactions of vermicompost and plant density had significant effect on oil content, oil density and refractive index of evening primrose oil. The best treatments were also including 2 Kg/m2 of vermicompost,20 plants/ m2in terms of oil production,9 plants/ m2in terms of oil quality and ratio of unsaturated fatty acids to saturated, respectively.
Material and Method: The aim of this research was to determine the effect of humic acid and bio-phosphor application on growth parameters in O. biennis. The experiment was conducted based on randomized complete block design with 8 treatments and three replicates during 2013-2014 growing season, at the experimental farm of the faculty of Agriculture, Ferdowsi University of Mashhad.. Treatments consisted of 4 levels of humic acid (0, 1, 3, 5 ml/L) and 2 levels of bio-phosphor (without bio-phosphor or with bio-phosphor). Liquid form of humic acid was used, which has low percentage of organic matters (16.5%) and applied as foliar spray after stem elongation in three stages with 10 days intervals until flowers stage. The use of bio-phosphor contain three strains of phosphate solubilizing bacteria(Pseudomonas pautida, Bacillus circulans and Bacillus subtilus) withCFU 108 numbers of spores (the number of living cells of each bacteria per milliliter). Analysis of variance was calculated using MSTATC andDuncan's multiple range tests was employed at probability level of 5%.
Results and Discussion: The results showed thatmaximum plant height (85.59 cm),number of seed per capsules on main shoot (251.17), oil percent (21.75 %) was obtainedby interaction effect between 5 ml/Lhumic acid and without bio-phosphor. Also, seed yield (1080 kg/ha) and oil yield (227 kg/ha) increased byinteraction effect between 3 ml/Lhumic acid and without bio-phosphor. Humic acid had a significant effect on increasing number of lateral branches (26/8), number of capsules on main shoot (117/35), as the highest value of all traits were obtained in 5 ml/L humic acid treatment. The highest number of capsules in lateral branches was observed in bio-phosphor treatment without humic acid. Bio-phosphor application had no significant effect on increasing oil percent. Oil yield was reduced by using of this fertilizer with 1ml/L humic acid treatment but application of 3 and 5 ml/L of humic acid showed no significant difference in comparison to control. Ahmed et al.reported that humic acid is a suspension based on potassium humate, which can be used as a plant growth stimulant in order to increase plant natural resistance against diseases and pests and subsequently increase plant yields. Phosphorus is an important element for cell division, root development and seed formation. Phosphate solubilizing bacteria produce organic acids which increase solubility of phosphorus and make it available for plants. Use of bio-fertilizers not only improves plant structure and activity of beneficial soil microorganisms, also it provides good availability of water and nutrients which finally improve plant yield. It can be said that foliar spray by humic acid stimulates plant growth and increaseevening primrose oil content.
Conclusions: Application of 3 and 5 ml/L of humic acid had the best results on traits andinteraction effect between humic acid and bio-phosphor showed positive effects on all traits except oil percent and oil yield. Generally, humic acid had positive effect on plant growth, oil yield, content and composition. Although, bio-phosphor had good effect on seed yield as a growth trait,its application with humic acid improved this trait.While bio-phosphor had no significant effect on oil content, it can be relative impact on oil composition.

کلیدواژه‌ها [English]

  • Phosphate solubilizig bacteria
  • Plant yield
  • Organic Culture
  • Organic fertilizers
  • Medicinal plant
1- Abdullah A.T.,Hanafy M.S., EL-Ghawwas E.O. and Ali Z.H. 2012. Effect of Compost and some Bio-fertilizers on Growth, Yield, Essential OilProductivity and Chemical Composition of Rosmarinusofficinalis L. Plants, Journal of Horticultural Science & Ornamental Plants, 4(2): 201-214.
2- Aftab A. and Asghari B. 2008. Rhizobium and Phosphate Solubilizing Bacteria Improve the Yield and Phosphorus Uptake in Wheat (Triticumsativum), International Journal of Agriculture & Biology, 10 (1): 85-88.
3- Ahmed Y.M., Shalaby E.A. and Shanan N. 2011.The use of organic and inorganic cultures in improving vegetative growth, yield characters and antioxidant activity of roselle plants (Hibiscus sabdariffa L.), African Journal of Biotechnology, 10(11): 1988-1996.
4- Aisha Ali H.,ShafeekM.R., MahmoudAsmaa R. and El- Desuki M. 2014. Effect of Various Levels of Organic Fertilizer and Humic Acid on the Growth and Roots Quality of Turnip Plants (Brassica rapa), Current Science International, 3(1): 7-14.
5- Ali E.and Hassan F. 2014. Bio-production of Nigella sativa L. seeds and oil in Taifarea, International Journal of Current Microbiology and Applied Sciences,3(1): 315-328.
6- Azizi M.,Nemati H. and Aroiee H. 2014. Effect of different levels of vermicompost and plant density on oil content and its component of evening primrose (Oenotherabiennis L.), Iranian Journal of Field Crops Research, 11(4): 608-617. (In Persian)
7- Azzaz N.A., Hassan E.A. and Hamad E.H. 2009. The Chemical Constituent and Vegetative and Yielding Characteristics of Fennel Plants Treated with Organic and Bio-fertilizer Instead of Mineral Fertilizer, Australian Journal of Basic and Applied Sciences, 3(2): 579-587.
8- Bakry M.A.A., Soliman Y.R.A. and Moussa S.A.M. 2009. Importance of micronutrients, organic manure and biofertilizer for improving Maize yield and its components grown in desert sandy soil, Research Journal of Agriculture and Biological Sciences, 5(1): 16-23.
9- Befrozfar M. R., Habibi D., Asgharzadeh A., Sadeghi-Shiae M.andTookalloo M.R. 2013. Vermicompost, plant growth promoting bacteria and humic acid can affect the growth and essence of basil (OcimumbasilicumL.), Annals of Biological Research, 4(2):8-12.
10- Christie W.W. 1999. The analysis of evening primrose oil, Industrial Crops and Products, 10:73–83.
11- DaneshvarHakimiMeybodi N., Kafi M., Nikbakht A. and Rejali F. 2012. The effect of humic acid on some quantitative and qualitative characteristics Speedy green grass, Iranian Horticultural Sciences, 42(4):403-412. (in Persian)
12- Das K., Dang R. andShivananda T.N. 2008. Influence of bio-fertilizers on the availability of nutrients (N, P and K) in soil in relation to growth and yield of Stevia rebaudiana grown in South India, International Journal of Applied Research in Natural Products, 1(1): 20-24.
13- El-Ghamry A.M., Abd El-Hai K.M. and Ghoneem K.M. 2009. Amino and Humic Acids Promote Growth, Yield and Disease Resistance of FabaBean Cultivated in Clayey Soil, Australian Journal of Basic and Applied Sciences, 3(2): 731-739.
14- El Gohary A.E., Gamal Mahmoud G.and Hussein M.S. 2013. Effect of Rock Phosphate vs. Biofertilizer on Growth, Yield, and Essential Oil Content of Menthalongifoliasubsp. SchimperiGrey, Journal of Applied Sciences Research, 9(11): 5912-5919.
15- FieldsendA.F. and Morison J.I. 2000. Contrasting growth and dry matter partitioning in winter and spring evening primrose crops (Oenotheraspp.), Field Crops Research, 68:9-20.
16- Ghasemnezhad A.andHonermeier B. 2007. Seed yield, oil content and fatty acid composition of Oenotherabiennis L. affected by harvest date and harvest method, Industrial Crops and Products, 25:274–281.
17- Ghasemnezhad A., Mousavizadeh S.J. and Mashayekhi K. 2011.A study on evening-primrose (Oenotherabiennis L.) callus regeneration and somatic embryogenesis, Iranian Journal of biothecnology, 9(1):31-36.
18- Gyvest E.M., Sparks C.A.,Fieldsend A.F.,Lazzeri P. and Jones H.D. 2001.High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron, Annals of Applied Biology, 138(3):329-332.
19- Habibi A., Heidari G.R., Sohrabi Y. and MohammadiKh. 2013. Effect of biofertilizers and chemical fertilizers on yield and yield components ofpumpkin (Cucurbitapepo L. Convar. pepo Var. styriaca), Iranian Journal of Medicinal and Aromatic Plants, 28(4):604-615. (in Persian with English abstract)
20- Ismail E.G., Mohamed W. W., Khattab S. and El sharif F. 2013. Effect of manure and bio-fertilizers on growth, yield, Silymarin content and protein expression profile of Silybummarianum, International Journal Medicine & Aromatic Plant, 3(4):430-438.
21- Jahan M. and NasiriMahalati M. 2014. Soil fertility and biofertilizers (Agroecological approach),Ferdowsi University of Mashhad, pp.11-21.
22- Jin S.S., Sumayoa M., Ye J.H., Byug S.K. and Sa Y.G. 2014. Screening of plant growth-promoting rhizobacteria as elicitor of systemic resistance against gray leaf spot disease in Pepper, Applied Soil Ecology, 73:1-8.
23- KamariShahmaleki S.,PeyvastGh. A. and Ghasemnejad M. 2013. The effect of humic acid on growth characteristics and yield of tomato Var. Isabella, Journal of Horticultural Science, 26(4):358-363. (in Persian).
24- Kaya M.,Ataki M., Khawari K.M., Ciftci C.Y. and Ozcan S. 2005.Effect of pre-sowing seed treatment with zinc and foliar spray of humic acids on yield of Common bean (Phaseolus vulgaris L.), International Journal of Agriculture and Biology, 7(6):875-878.
25- Khazaie H.R., EyshiRezaie E. and Bannayan M. 2011. Application times and concentration of humic acid impact on aboveground biomass and oil production of hyssop (Hyssopusofficinalis), Journal of medicinal Plants Research, 5(20):5148-5154.
26- KoochakiA., Tabrizi L. and Ghorbani R. 2008.The evaluate the effect of biofertilizers on growth characteristics, yield and quality of Hyssop (Hyssopusofficinalis), Iranian Journal of Field Crops Research, 1(6):127-137. (in Persian).
27- Mayi A.A., Ramazan Ibrahim Z. and Abdurrahman A. S. 2014. Effect of foliar spray of humic acid, ascorbic acid, cultivars and their interactions on growth of Olive (Olea European L.) Transplants Cvs. Khithairy and Sorany, IOSR Journal of Agriculture and Veterinary Science, 7(4):18-30.
28- Mohammadipour E., Golchin A., Mohammadi J.,Negahdar N. and Zarchini M. 2012. Improvement Fresh Weight and Aerial Part Yield of Marigold (Calendula officinalis L.) by Humic Acid, Annals of Biological Research, 3(11):5178-5180.
29- Mohammadvarzi R., Habibi D., Vazan S. and PazeI.A. 2011. Effect of PGPR and nitrogen fertilizer on sunflower seed quality(Helianthus annus L.), Journal of Crop Eco-physiology, 2(3):156-167.
30- Montserrat-de la paz S., Fernandez-Arche M.A., Angel-Martin M. and Garcia-Gimenez M.D. 2014. Phytochemical characterization of potential nutraceutical ingredients from Evening Primrose oil (Oenotherabiennis L.),Phytochemistry Letters, 8:158–162.
31- MoosaviS.Gh.,Sedaghatoleslami M.J.,Jouyban Z. and Ansarinia E. 2012. Germination and growth parameters of seedlings of Oenotherabiennis L. as affected by salinity stress, Technical Journal of Engineering and Applied Sciences, 2(5):123-127.
32- NematiDarbandi H. 2011. Effect of different levels of vermicompost and plant density on quantitative and qualitative characteristics in evening primrose, MSc-Thesis, FersowsiUniversity of Mashhad.
33- Omidbaigi R. 2010. Production and Processing of Medicinal Plants, p.352-362, Part 13, Evening primrose,Behnashr publication.
34- Osman E.A., M. EL-Masry A. A. and Khatab K. A. 2013. Effect of nitrogen fertilizer sources and foliar spray of humic and/or fulvic acids on yield and quality of rice plants, Advances in Applied Science Research, 4(4):174-183.
35- Park T. 2009. Chemical information review document for Evening Primrose oil (Oenotherabiennis L.), National Toxicology Program, November 2009.
36- Pedram M., Ayenehband A. and Modhej A. 2013. The effect of biological and chemical fertilizers and plant density onquality and quantity yield of Safflower (CarthamustinctoriusL.) under Ahvaz condition, International journal of Agronomy and Plant Production, 4(3):524-529.
36- Rezae M. and Baradaran R. 2013. Effects of bio fertilizers on the yield and yield components of pot marigold (Calendula officinalis L.), Iranian Journal of Medicinal and Aromatic Plants, 29(3):635-650.(in Persian with English abstract).
37- RezaieMoadabA. and NabaviKalat S.M. 2013. The effect of vermicompost and organic fertilizer on seed yield and yield components of basil (Ocimumbasilicum L.), Journal of Crop Eco-physiology, 6(2):157-170. (in Persian).
38- Sadeghimoghaddam S., Molafilabi A., Armin M. and Moslehi J. The effects of chemical and organic fertilizers on yield and yield components of cumin. 2nd National Conference on sustainable development of agriculture and the environment healthy, 21 Sep. 2013, Technical College ofShahidMofateh of Hamadan, (in Persian).
39- SaeednejadA.H. and Rezvanimoghaddam P. 2010. Evaluate the effects of biological and chemical fertilizers on morphological characteristics, yield, yield components and the percentage of essential oil of cumin(Cuminumcyminum), Journal of Horticultural Science, 24(1):38-44. (in Persian)
40- Said-Al Ahl H., Ayad H.S. and Hendawy S. F. 2009.Effect of potassium humate and nitrogen fertilizer on herb and essential oil of oregano under different irrigation intervals,Ozean Journal of Applied Sciences, 2(3):319-323
41- Sandeep K., Piyush P. andMaheshwari D.K. 2009. Reduction in dose of chemical fertilizers and growth enhancement of sesame (Sesamumindicum L.) with application of rhizospheric competent Pseudomonas aeruginosaLES4, European Journal of Soil Biology, 45:334–340.
42- SekerogluN. and Ozguven M. 2006. Effects of different nitrogen doses and row spacing applications on yield and quality of (Oenotherabiennis L.)grown in irrigated lowland and un-irrigated drylandconditions, Turkish Journal of Agricultural and Forestry, 30:125-135.
43- Shehata M.M. and El-Khawas S.A. 2003. Effect of two bio-fertilizers on growth parameters, yield characters, nitrogenous components, nucleic acid content, minerals, oil content, protein profiles and DNA banding pattern of sunflower (Helianthus annus L. cv. Vedock) yield, Pakistan Journal of biological science, 6(14):1257-1268.
44- TahamiZarandi S.M.K.,Rezvanimoghadam P. and Jahan M. 2010. Effect of compost of city rubbish and some biologic fertilizers on yield, seed yield component and harvest index in sweet basil. (Ocimumbasilicum), 1st agriculture and stable development national congress, 10-11 March.Islamic Azad University, Shiraz. (In Persian)
45- Veisi H.,HeidariGh.R. and Sohrabi Y. The effect of mycorrhiza fungi and humic acid fertilizerindifferent levels on yield and quality of Sunflower, 2nd National Conference on sustainable development in arid and semi-arid, 4-5 Des 2013. Abarkuh.
46- Vilela A., Gonzalez-Paleo L.,Rondanini D. and Ravetta D. 2008. Biomass allocation patterns and reproductive output of four Oenothera L. accessions native to Argentina, Industrial Crops and Products, 27:249–256.
47- Zare Y., Faravani M. and Koorepaz S. p 26. Effect of biofertilizers on growth, yield and yield component of Black Cumin(Nigella sativa L.), National conference on natural products and medicinal plants, 27-28 Sep. 2012, University of Medical Sciences of North Khorasan,Bojnoord. (in Persian).
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