اثر تیامین، اسید آسکوربیک و اسید جیبرلیک، بر برخی شاخص های رشد، میزان رنگیزه‌ها و قند احیاء در گل اطلسی

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه شهید باهنر کرمان

چکیده

گیاهان بستر ساز از جمله اطلسی با توجه به تنوع عادت رشد و رنگ از عناصر بسیار مهم فضای سبز در شهرها به شمار رفته و بسیار مورد توجه طراحان فضای سبز قرار گرفته است. این پژوهش روی گل اطلسی با هدف افزایش شاخص های رشد (طول شاخه جانبی، طول ریشه، قطر ساقه، قطر گل، تعداد گل، تعداد شاخه جانبی) و صفات بیو شیمیایی (کلروفیل a، کلروفیل b، کلروفیل کل، کارتنوئید، قند احیا)، در قالب آزمایش فاکتوریل بر پایه طرح کاملاً تصادفی با 5 تکرار اجرا گردید. فاکتورها بر این اساس که دو ویتامین و یک تنظیم کننده رشد (اسید آسکوربیک، تیامین و اسید جیبرلیک هر سه با غلظت صفر و 100 میلی‌گرم در لیتر) مورد استفاده قرار گرفت. بر اساس نتایج حاصل، GA3 نسبت به دو ویتامین کاربردی تاثیر قابل توجهی بر کلیه شاخص های اندازه‌گیری شده به جز کارتنوئید داشت و همچنین اثرهای ترکیبی GA3 و اسید آسکوربیک بر شاخص های مرتبط با رشد ساقه‌ها، ریشه و قطر گل و ترکیب GA3 و تیامین بر رنگیزه‌ها، میزان قند، کارتنوئید و تعداد گل در میان تیمارهای دو گانه قابل توجه بود. در کلیه شاخص‌های اندازه‌گیری شده در این پژوهش، اثرهای متقابل دو گانه و سه گانه معنی‌دار شد و در نهایت ترکیب توام هر سه ماده کاربردی (اسید آسکوربیک، تیامین و اسید جیبرلیک هر سه با غلظت 100 میلی‌گرم در لیتر) موجب افزایش قابل توجه شاخص‌های مرتبط با رشد و نمو ریشه و ساقه‌ها، تعداد و قطر گل، رنگیزه‌ها و قند احیاء نسبت به شاهد و دیگر تیمارها شد و به عنوان بهترین تیمار، پیشنهاد گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Thiamine, Ascorbic acid and Gibberellic acid (GA3) on Growth Characteristics, Pigment Content and Reduced Sugars of Petunia

نویسندگان [English]

  • moslem salehi
  • Vahid reza Saffari
  • shima hasanzadeh fard
Shahid Bahonar University of Kerman
چکیده [English]

Introduction: Bedding plants, especially petunia is important element for urban landscaping and attracted the attention of landscapers. This is due to some properties such as growth habit and color. The petunia (Petunia hybrida L.) belongs to Solanaceae family that has annual and perennial varieties. This plant is originally from Argentina, Brazil and Uruguay. Some plant growth regulators such as gibberellic acid (GA3) and vitamins including thiamine and ascorbic acid affect plant growth and development and may extend flowering period. Vitamin C affects cell division and cell growth in plants and is effective on the feeding cycle activity in higher plants and it has an important role in electron transport system. The concentrations of 50 and 100 ppm of vitamin C and thiamine can increase the plant height, leaf number, leaf area, fresh and dry weight, and chemical compounds of the Syngonium plant. The application of 100 mg/l of GA3 significantly increased plant height and the number of leaves of gladiolus.
Material and methods: The experiment was arranged in a factorial based on a completely randomized design with five replications. In this research, growth characteristics (lateral branch number, flower number, flower diameter, stem diameter, root length, and lateral branch length) and biochemical characteristics (chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, and reducing sugar) were measured. After seeding and transplanting the seedling at 6 leaf stage, plants sprayed at 4 various growth stages with following treatments:
1- Control
2- Vitamin C(100 mg/l)
3- Thiamin (100 mg/l)
4- Gibberellic acid (100 mg/l)
5- Vitamin C and Thiamin (The concentration of both 100 mg/l)
6- Vitamin C and Gibberellic acid (The concentration of both 100 mg/l)
7- Thiamin and Gibberellic acid (The concentration of both 100 mg/l)
8- Vitamin C, Thiamin and Gibberellic acid (The concentration of three 100 mg/l)

Data obtained from the measured parameters analyzed using statistical package SAS and means were compared using LSD test at the level of statistical significance of P˂0.05.
Results and Discussion: The results indicated that GA3 affected all the measured characteristics except carotenoids in comparison with thiamine and ascorbic acid. Meanwhile, the combined application of GA3 and ascorbic acid affected stem growth, root growth and flower diameter. Simultaneous use of GA3 and thiamine was effective on pigments, reducing sugar, carotenoids and flower number. The simultaneous application of three chemicals resulted in a considerable increase in growth and development traits, such as stem and root growth, flower number and diameter, pigments and reducing sugar compared to control. Generally, gibberellic acid will increase vegetative growth with impress cellular processes, including stimulation cell division and cell elongation. It was reported that the uses of ascorbic acid and gibberellic acid in Thyme (Thymus vulgaris) increased plant height and root length. Ascorbic acid makes a set of functions such as cell division and enlargements, cell wall development and other developmental processes in plants. Ascorbic acid affects plasma membrane proton pump and according to the acidic theory stimulates weaking cell wall and consequently will increase cell wall development and cell enlargement. It was found that thiamine significantly increased flower number in chrysanthemum .
Conclusion: The results indicated that the synergistic effects of this material can be used to enhance the growth and development of petunia. On the other hand, in arid and semiarid areas of the country, that drought and salinity stresses somewhat inevitable, these materials, especially vitamins are effective in reducing stress and increase flowering period.

کلیدواژه‌ها [English]

  • Flowering
  • Growth regulator
  • Reduced sugars
  • Spray
  • Vitamin
1- Abdel-Aziz N.G., El-Quesni Fatma., E.M., and Farahat M.M. 2007. Response of vegetative growth and some chemical constituents of Syngonium podophyllum L. to foliar application of thiamine, ascorbic acid and kinetin at Nubaria. World Journal of Agricultural Science, 3: 301-305.
2- Abdel-Aziz N.G., Mazher, A.A.M., and Farahat M.M. 2010. Response of vegetative growth and chemical constituents of Thuja orientalis L. plant to foliar application of different amino acids at Nubaria. Journal of American Science, 6: 295-301.
3- Abdel-Aziz N.G., Taha Lobna S., and Ibrahim Soad M.M. 2009. Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of Gladiolus plants at Nubaria. Ozean Journal of Applied Sciences, 2(2): 169-179.
4- Akbari S., and Moalemi N. 2011. The effect of gibberellic acid on the growth of olive trees (Olea europaea L.). Journal of Horticultural Science, 24(2):184-188. (In persian)
5- Amin A.A., Rashad E.M., and Gharib A.E. 2008. Changes in morphological, physiological and reproductive characters of Wheat plants as affected by foliar application with Salicylic acid and Ascorbic acid. Australian Journal of Basic and Applied Sciences, 2(2): 252-261.
6- Antonopoulo Ch., Dimassi K., Therios I., Chatzissavvidis, Ch., and Tsirakoglou V. 2005. Inhibitory effects of riboflavin (Vitamin B2) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF 667 (Prunus amygdalus × P. Persica). Scientia Horticulturae, 106: 268-272.
7- Bedour, A.A., and Eid R.A. 2011. Improving gladiolus growth, flower keeping quality by using some vitamins application. Journal of American Science, 7: 169-174.
8- Ben-Jaacov J. 2006. Gibberellic acid spray increased size and quality of “Protea Pink” Ice Flowers. Acta Horticulture, 716:135-140.
9- Blokhina O., Virolainen E., and Fagerstedt K.V. 2003. Antioxidant, oxidative damage and oxygen deprivations stress. Review in Annals of Botany, 91:179-194.
10- Dhaduk B.K., Kumara S., Singh A., and Desai J. R. 2007. Response of gibberellic acid on growth and flowering attributes in anthurium (Anthurium andreanum L.). Journal Horticultural Science, 10: 187-189.
11- Dolaat-Adadian A., Modaress-sanavi S.A., and Sharifi M. 2010. Effects of water stress and ascorbic acid sprayed on the activity of antioxidant enzymes and some biochemical changes in corn leaves (Zea maize L.). Iranian Journal of Biology, 22(3): 407-421. (In persian)
12- El-Fawakhry F.M., and El-Tayeb H.F. 2003. Effect of some amino acids and vitamins on Chrysanthemum production. Agricultural Research Alexandria University, 8: 755-766.
13- El-Lelhy S.R., Ayad H.S., and Reda F. 2011. Effect of riboflavin, ascorbic acid and dry yeast on vegetative growth, essential oil pattern and antioxidant activity of geranium (Pelargonium graveolens L.). American Eurasian Journal of Agricultural and Environmental Sciences, 10: 633-638.
14- El-Quesni Fatma E.M., Abd El-Aziz, N.G. and Kandil M.M. 2009. Some studies on the effect of ascorbic acid and a-tocopherol on the growth and some chemical composition of Hibiscus rosa sincses L. Ozean Journal of Applied Sciences, 2: 159-167.
15- Ghasemi Qhsareh M., and Kafi M. 2008. Floricultural scientific and practical. Golbon publications. 1: p. 335. 2nd ed. (In persian)
16- Hassanpour-Asil M., Mortazavi S.H., Hatamzadeh A., and Ghaseminejad M. 2013. Effects of gibberellic acid and calcium in reducing the growth of lily (Iris hollandica var. Blue Magic) in greenhouses and increase the shelf life of cut flowers. Science and Technology of Greenhouse Culture, 3(9): 63-70. (in persian)
17- Hekmati J. 2004. Seasonal flowers (Flowers outdoors). Publication of Agricultural Sciences Publications. p. 285. 1nded. (In persian)
18- Hendawy S.F., Ezz E.L., and Din A.A. 2010. Growth and yield of Foeniculum vulgar var. azoricum as influenced by some vitamins and amino acids. Ozean Journal of Applied Sciences, 3(1): 113-123.
19- Horemans N., Foyer C.H., Potters G., and Asard H. 2000. Ascorbate function and associated transport system in plants. Plant Physiology and Biochemistry, 38:531-540.
20- Kheyri A., Khalighi A., Mostophi Y., and Naderi R. 2012. The effect of different concentrations of gibberellin and 6-BA on quantitative and qualitative characteristics tuberose. Journal of Crops Improvement, 13(1): 9-20. (in persian)
21- Khoshkhooy M. 2013. New Principles of Gardening. Shiraz University Publications. p. 638. (in persian)
22- Kishan S., Singh, K.P. and Raju D.V.S. 2007. Vegetative growth, flowering and seed characters of African marigold (Tagetes erecta L.) as influenced by different growth substances during mild of seasons. Journal Ornamental Horticulture, 10: 268-270.
23- Leena R., Rajeevan P.K., and Valasala K.P.K. 1992. Effect of foliar application of growth regulators on the growth, and flowering and corm yield of gladiolus cv. Friendship. South Indian Horticulture, 40: 329-335.
24- Lichtenthder H.K. 1987. Chlorophylls and Carotenoids Pigments of Photosynthetic biomemberanes. Methods in Enzymology, 148: 350-382.
25- Magome H., Yamaguchi S., Hanada A., Kamiya Y., and Odadoi K. 2004. Dwarf and delayed flowering, a novel Arabidopsis mutant deficient in gibberellins biosynthesis because of over expression of a putative AP2 transcription factor. Plant Journal, 37: 720-729.
26- Mahgoub H.M., Abdel Aziz G.N., and Mazhar M.A. 2011. Response of Dahlia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American Eurasian Journal of Agricultural and Environmental Sciences, 10: 769-775.
27- Majidian N., Naderi R., Khalighi A., and Majidian M. 2013. The Effect of Four Levels of GA3 and BA on the Quantitative and Qualitative Characteristics of Zantedeschia aethiopica cv. Childsiana Pot Plant. Journal of horticulture science, 25(4): 361-368. (in persian)
28- Miguel A., Rosales Z., Juan M., Ruiz A., Hernandez J., Soriano T., Castilla N., and Romero L. 2006. Antioxidant content and as ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation. Journal of the Science of Food and Agriculture, 86: 1545-1551.
29- Mohamed G.F., and Abdella E.M.M. 2013. Response of Calendula officinalis L. plants to foliar application of gibberellic acid and mixture of some micronutrients. Journal of Applied Sciences Research, 9: 735-742.
30- Nahed G.A., El-Aziz A., Fatma E.M., and Farahat M.M. 2007. Response of vegetative growth and some chemical constituents of Syngonium podophyllum to foliar application of Thiamine, Ascorbic acid and Kinetin ot Nurbaria. World Journal of Agricultural Science, 3(3): 301-305.
31- Panwar R.D., Sindhu S.S., Sharma J.R., and Saini R.S. 2006. Effect of gibberellic acid spray on growth, flowering, quality and yield of bulbs in tuberose. Haryana Journal Horticultural Science, 35(3-4): 253-255.
32- Pazuky A., Rezaei H., Habibi D., and Pak-nejad F. 2013. Effects of drought stress, ascorbate and gibberellin spray on some morphological traits, relative water content and stability of the cytoplasmic membrane Thyme (Thymus vulgaris L.). Journal of Agronomy and Plant Breeding, 8(1): 1-13. (in persian)
33- Prabhat Kumar R.S.P.S., Mishra R.L., and Singh K.P. 2003. Effect of GA3 on growth and yield of china aster (Aster chinensis). Journal Ornamental Horticulture, 6: 110-112.
34- Sandeep Tyagi T.A.K., kumar V., and Kumar N. 2008. Effect of GA3 and IAA on growth, flowering and yield of calendula (Calendula officinalis L.). Program Agricultural, 8: 118-120.
35- Singh M.P., Singh R.P., and Singh G.N. 1991. Effect of GA3 and ethrel on the growth and flowering of African margold (Tagetes erecta L.). Haryana Journal Horticultural Science, 20: 81-84.
36- Smiroff N., and Wheeler G.L. 2000. Ascorbic acid in plants: biosynthesis and function. Critical Reviews in Plant Sciences, 19: 267-290.
37- Soad M.M., Taha I.L.S., and Farahat M.M. 2010. Vegetative growth and chemical constituents of croton plants as affected by foliar application of benzyl adenine and gibberellic acid. Journal of American Science, 6: 126-130.
38- Somogy M. 1952. Note son sugar determination. Journal of Biochemistry, 195: 19-29.
39- Umrao V.K., Sharma V., and Kumar B. 2007. Influence of gibberellic acid spraying on gladiolus cv. Rose Delight. Program Agricultural, 7: 187-188.
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