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مجتبی لطفی محمد فارسی امین میرشمسی کاخکی جواد جانپور

چکیده

القای تشکیل ساختار ته¬سنجاقی در قارچ Agaricus bisporus به طور خاص، با کاهش دما و غلظت دی¬اکسیدکربن در حضور باکتری¬های موجود در خاک پوششی از جمله باکتری Pseudomonas putidaانجام می¬شود. این باکتری می¬تواند به عنوان محرک رشد بر روی عملکرد قارچ دکمه¬ای نیز تأثیر بگذارد. در این پژوهش 81 جدایه باکتری از نمونه¬های خاک پوششی 6 مزرعه پرورش قارچ خوراکی در سال 1394 جداسازی شد و در نهایت 33 جدایه که به عنوان باکتری P. putida شناسایی شدند، به خاک پوششی قارچ دکمه¬ای تلقیح گردیدند. نتایج آزمون¬های مزرعه¬ای نشان داد که جدایه¬های مورد بررسی نسبت به تیمار شاهد (بدون اعمال باکتری) تأثیر معنی¬داری بر روی وزن تر و تعداد قارچ دارند (p≤0.05)؛ به طوری که بیشترین وزن تر قارچ مربوط به تیمار جدایه¬های P27 وP13 به ترتیب به میزان 63/361، 8/342 گرم (تیمار شاهد 39/146 گرم) و بیشترین تعداد قارچ مربوط به تیمار جدایه¬های P18 و P24 به ترتیب با 21 و 83/20 عدد (تیمار شاهد 50/8 عدد) بر کیلوگرم کمپوست بود. در مرحله بعد توان تولید سیدروفور، توان تولید هورمون IAA، فعالیت آنزیم ACC دآمیناز و توانایی انحلال فسفات نامحلول در جدایه¬ها ارزیابی و رابطه هرکدام از آن¬ها با تعداد و وزن تر قارچ مورد بررسی قرار گرفت. نتایج نشان داد که بین میزان تولید هورمون IAA و وزن تر قارچ (58/0=r) و همچنین بین تولید هورمون IAA و تعداد قارچ (50/0=r) همبستگی معنی¬دار و مثبتی وجود دارد. پس می¬توان نتیجه گرفت که تولید IAA توسط باکتری ممکن است عامل تأثیرگذاری بر روی عملکرد قارچ دکمه¬ای ¬باشد.

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ارجاع به مقاله
لطفیم., فارسیم., میرشمسی کاخکیا., & جانپورج. (2018). بررسی تأثیرجدایه‌های باکتری Pseudomonas putida بر عملکرد قارچ خوراکی دکمه¬ای سفید (Agaricus bisporus). علوم باغبانی, 32(2), 273-286. https://doi.org/10.22067/jhorts4.v32i2.62530
نوع مقاله
علمی - پژوهشی