Effects of BA and TDZ on bud growth and immature cotyledons somatic embrygenesis of late flowering almond (Prunus dulcis L.) ‘7-Sharood’ cultivar

Document Type : Research Article



In this research, the effects of BA with different concentrations (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg/l) in combination with IBA (0, 0.01 and 0.1 mg/l) on bud growth and shoot proliferation and TDZ with different concentrations (0, 1, 2, 3 and 4 mg/l) in combination with 0 and 0.5 mg/l IBA on somatic embryogenesis from different parts of immature cotyledons (proximal, median and Distal) were investigated. The results showed that the highest rate of bud growth and shoot proliferation were obtained in 2 mg/l BA and 0.01 mg/l IBA (3.85 shoots per explant). TDZ as a potent cytokinin caused initiation of somatic embryogenesis in proximal and median parts of cotyledons. The highest percentage of embryogenesis occurred in proximal part with 4 mg/l TDZ (70%) and using IBA plus TDZ decreased the percentage of embryogenesis.

Key words: Cotyledons ,Shoot proliferation, Somatic embryogenesis