Journal of Horticultural Science
with the collaboration of Iranian Scientific Association for Landscape (ISAL)

Effects of Sterilization Protocol and Pre-chilling Treatment on In vitro Seed Germination of Levisticum officinale Koch.

Document Type : Research Article

Authors

Raheleh Khatibzadeh
Majid Azizi
Hossein Arouiee
Mohammad Farsi

Ferdowsi University of Mashhad

https://doi.org/10.22067/jhorts4.v0i0.24810
Abstract
To protect and multiply important and rare plant resources, in vitro culture serves as a more efficient alternative to traditional propagation approaches. Levisticum officinale Koch. a member of Apiaceae is an important, endangered and neglected species in Iran, which has been shown to have diuretic, spasmolytic and carminative effects. In order to supply enough plant materials for micro-propagation of this herb and study effects of different methods of disinfection and stratification on in vitro seed germination, a factorial experiment laid out in a completely randomized design was set out to establish sterile plants out of seed culture. It was concluded that a pre-chilling treatment for 3 months resulted in maximum percent of germination (92%) and the largest germination rate. The best superficial sterilization protocol was proofed to be soaking in 70% (v:v) ethanol for 30 s and then, using of 2% (v:v) dilution of NaOCl for 15 min, followed by 3 rinses in sterile distilled water.

Keywords

Levisticum officinale
Medicinal plants
Seed germination
In vitro culture
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Journal of Horticultural Science
Volume 27, Issue 2 - Serial Number 2
Summer 2013
Pages 130-138

  • Receive Date 14 August 2013
  • First Publish Date 23 August 2013

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