Effect of Population Type and BAP Treatments on In vitro Regeneration of Hyssop (Hyssopus officinalis L.)

Document Type : Research Article



Hyssop (Hyssopus officinalis L.), belongs to the family of Lamiaceae which its origin is Asia Minor, and is considered as one of the most important medicinal plant. This experiment was done to study the effects of population and BAP concentrations on hyssop plant regeneration under in vitro culture conditions. This research were performed in factorial experiment with three populations (Hamadan, Shiraz and Mashhad) and four levels of BAP (0, 2.2, 4.4, 13.2 µM), in three replications. Results showed that there is significant difference between populations. In all three populations, the lowest average and percentage of regeneration was observed in MS medium without any growth regulators. In Hamadan population, the highest average of regenerated shoots (7.86) and percentage of regeneration (86%) was obtained in BAP (4/4 µM) and in Shiraz population, maximum number of regenerated shoots (7) and 80% regeneration percentage was observed on 4.4 µM BAP. The minimum regeneration percentage with 0% was obtained in MS medium without plant growth regulators and MS media supplemented with 13.2 µM concentration of BAP. 2.2 µM BAP in Mashhad population with an average of 9 regenerated shoots and 86% regeneration percentage was known as the best treatment. ANOVA results showed significant (P < 0.01) effect of IBA levels on root induction. The highest rooting percentage (86.66%) was achieved in MS medium supplemented with 9.84 µM of IBA and the lowest rooting percentage (0%) was observed in 1/2 MS hormone-free medium.