Optimizing of in vitro Zygotic Embryo Culture of Salvia leriifolia Benth.

Document Type : Research Article

Authors

• M. Modarres ¹
• mehrdad lahouti
• Ali Ganjeali
• J. Asili ²

¹ Shahid Hasheminejad Campus -Farhangian University of Mashhad

² Faculty of Pharmacy, Mashhad University of Medical Sciences

10.22067/jhorts4.v0i0.42782

Abstract

Salvia leriifolia (Lamiaceae) is endemic of Khorasan and Semnan province and an endangered plant. Poor seed germination of this plant has a serious problem with the high production. The first step to improve this precious plant is to produce sterile plantlets in order to prepare explants of appropriate vigor. In this study,in vitro culture of Salvia leriifolia zygotic embryo was performed through a factorial experiment in the form of completely random design including culture medium, BAP and NAA. The results showed that, MS and 1/2MS media appeared to be more efficient than B5 medium and significant differences were observed. Efficient concentrations of BAP and NAA were 1mgL-1 and had a significant effect in growth and development of embryos. The seedlings were obtained 10 days after planting. Based on these results, the best choice for quick access to strong seedlings, seedling growth and development of embryos is MS and 1/2MS media supplemented with 1mgL-1 BAP and NAA.

Keywords

• Optimizing
• Embryo culture
• Salvia leriifolia

20.1001.1.20084730.1393.28.3.6.1

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