The Effect of Potassium on the Controlling of Salt in Evening Primrose (Oenothera macrocarpa)

Document Type : Research Article


Ferdowsi University of Mashhad


Introduction: Salinity has been recognized as one of the major abiotic factors affecting crop yields in arid and semi-arid irrigated areas of the world and efforts for breeding salt-resistant crop plants have been made. Approximately one-third of the world irrigated soils and a large proportion of soils in dry land are saline. Two major effects have been identified as the probable causes of high salt toxicity in crop plant i.e., the ionic effect and the osmotic effect. The ionic effect results in alterations in enzymatic processes, disturbances in accumulation and transport of different ions or a combination of all these factors. As a result, shoot and root growing reduce and uptake of nutrient elements by plants is adversely affected. While excess Na accumulated in plants under salinity stress conditions hinders K uptake; Cl hinders NO3 uptake by plants and destroys ionic balance in plants. Evening primrose is a plant which belongs to Onagraceae. Its seed oil has a special arrangement in Glycerol molecule, so it has been used a lot in medical treatments and also feeding. Researchers showed that using the best techniques and methods in farming can increase the amount of oil in the seeds of this plant. The wrong method of agricultural activities in Iran caused increasing salt in the soil, so growing plants in this situation isn’t possible. For confronting with this phenomenon knowing and choosing kinds of plants that can resist the situation of salt is really a necessary.
Materials and Methods: This study was conducted as a factorial experiment based on completely randomized design with three replicates was performed with five levels of NaCl salinity on Oenothera macrocarpa (0, 30, 60, 90 and 120 mM) and potassium chloride levels (zero and 15.02 mM) and three times in the Faculty of Agriculture, Ferdowsi University of Mashhad in 1390. Salt treatment to prevent osmotic shock was applied to four-leaf stage and treated with potassium was gradually simultaneously with irrigation water applied. 6 weeks after the treatments, the rate of photosynthesis, chlorophyll relative content and stomata conductance was measured. The analysis of variance was estimated using SAS software. The statistical comparison was done by Duncan's multiple range tests. Charts were drawn using Excel software.
Results and Discussion: According to the result of the analysis of variance, increasing the density of sodium chloride in the planting areas had a special effect on the size of the leaves and the weight of dried plant and the weight of each leaf and dried root. This effect showed a meaningful variation between the weight of dried leaves and its dried root and shoots. The salty areas have a lot of negative ions like Magnesium, Chlorine, sodium and sulfate. These materials are harmful by themselves or cause effective disorder in the plants metabolism. Salinity treatments applied to significant influence (01/0> p) on the characteristics of photosynthesis, stomata conductance and numbers were read out by spade. For example, sodium and potassium competition and competition between chlorine and nitrate impairs the absorption of nutrients. The result of this reaction is that the plant needs more energy for producing organic matter so it loses most of its energy to resist against salt. This situation of the plant causes a low activity of the root and the growing of the shoot consequently reduce. At this situation the weight and length of the plant reduce too. For example existing potassium in salty lands cause the reduction of sodium in the shoot of the plants. This research was done in a pot with the same amount of salt. Potassium causes the reduction of Toxicity effects of sodium. Research has shown that the potassium in regulating osmotic pressure and permeability of plant cell membranes is effective and cause Increase plant tolerance to salinity.
Conclusion: Some biological indexes of evening primrose plant were negatively affected by increasing rates of NaCl and KCl applications. In salty condition, increasing the amount of sodium causes the reduction of potassium, comparing with sodium. As a matter of fact, this kind of reaction causes the reduction of potassium comparing with sodium. We know that potassium causes a suitable osmotic pressure and reduce the destructive effect of oxidation. So having more potassium than sodium in salty lands is known as the standard resistance. NaCl and KCl compounds should be applied in a precise amount to evening primrose. Otherwise, quantity and quality of evening primrose plant will be decreased.


-Abid, M., Qayyum, A., Dasti, A. A., and Abdilwajid R. 2001. Effect of salinity and SAR of irrigation water on yield, physiological growth parameters of Maize & properties of the soil. Journal Research., 12(1):26-33.
2-Apse, M. P., and Blumwald, E. 2002. Engineering salt tolerance in plant. Journal Biotechnology. 13: 146-150.
3-Asch, F., Dingkuhn, M., and Dorffling K. 2000. Salinity increases CO2 assimilation but reduces growth in field grown, irrigated rice. Plant and Soil. 218:1-10.
4-Ashraf, M., and Orooj, A. 2005. Salt stress effects on growth, ion accumulation and seed oil concentration in an arid zone traditional medicinal plant ajwain (Trachyspermum ammi L. Sprague). Department of Botany, University of Agriculture, Faisalabad 38040, Pakistan.
5-Ashraf, M., Mukhtar, N., Rehman, S. and Rha E. S. 2004. Salt induced changes in photosynthetic activity and growth in a potential medicinal plant Bishop`s weed (Ammi majus L.). Photosynthetica. 42(4): 543-550.
6-Bernstein, N., Kravchik, M., and Dudai, N. 2009. Salinity-induced changes in essential oil, pigments and salts accumulation in sweet basil (Osimum basilicum) in relation to alteration of morphological development. Annals Applied Biology. 156(2): 167-177.
7-Bhandal, IS, and Malik, CP. 1988. Potassium estimation, uptake, and its role in the physiology and metabolism of flowering plants. International Review of Cytology. 110: 205-254.
8-Cakmak, I. 2005. The role of potassium in alleviating detrimental effects of abiotic stresses in plants. Plant Nutrition and Soil Science. 168: 521-530.
9-Chow, W.S., Ball, M.C., and Anderson, J.M. 1990. Growth and photosynthetic responses of spinach to salinity implications of K+ nutrition for salt tolerance, Australian Journal of Plant Physiology., 17: 563 578.
10-Court, W.A., Hendel, J.G., Pocs, R. 1993. Determination of the fatty acids and oil content of evening primrose (Oenothera biennis L). Food Research. 26:181-186.
11-Cuin, TA., Miller, AJ., Laurie, SA., and Leigh, RA. 2003. Potassium activities in cell compartments of salt-grown barley leaves. ExperimentBotany. 54: 657- 661.
12-De Araujo, S.A.M., Silveira, J.A.G., Almeida, T.D., Rocha, I.M.A., Morais D.L., and Viegas, R.A. 2006. Salinity tolerance of halophyte (Atriplex nummularia L.) grown under increasing NaCl levels, Revista Brasileira de Engenharia Agricola e Ambiental, 10: 848-854.
13-Drazkiewicz, M. 1994. Chlorophyllase: Occurance functions, mechanism of action, effects of external and internal factors. Photosynthesis, 30:321-331.
14-Fieldsend, A.F., and Morison, J.I.L. 2000 b. Contrasting growth and dry matter partitioning in winter and spring evening primrose crops (Oenothera spp.). Field Crops Research., 68:9-20.
15-Gramer, G.R., Alberico, G.J. and Schmidt, C. 1994. Salt tolerance is not associated with the sodium accumulation of two maize hybrids. Australian. Journal Plant physiology., 21(5): 675-682.
16-Guo, F.O., and Tang, Z.C. 1999. Reduced Na+ and K+ permeability of K+ channel in plasma membrane isolated from roots of salt tolerant mutant of wheat, Chinese Science Bulletin, 44: 816-821.
17-Haghnia, G.H. 2004. Plant tolerance to salinity. Mashhad university publishers.
18-Hall, A.F. 2001.Crop responses to enviromental stresses. 232 p.
19-Hasni, I., Ben Ahmed, H., Bizid, E., Raies A., Samson, G. and Zid, E. 2009. Physiological characteristics of salt tolerance in fenugreek (Trigonella foenum graecum L.). The Proceedhngs of the International Plant Nutrition. Colloquim XVI, UC Davis.
20-Hejazi, A., Shahroodi, M., and Ardfroosh, J. 2004. Analytical methods of plant. The first edition of Tehran University Press.
21-Hoseyni, H.,and Rezvani, P. 2006. Salt and drought stress on the germination of plantago ovate. Crop Research Journal 4(1):15-25.
22-Hung, I., and Redman, R. E. 1995. Solute adjustment to salinity and calcium supply in cultivated and wild barley. Plant Nutrition. J. 18:1371-1389.
23-Jamil, M., Lee, D. B., Jung, K. Y., Ashraf, M., Lee, S. C., and Rha, E. S. 2006. Effect of salt (NaCl) stress on germination and early seedling growth of four vegetable species. Journal Central European Agriculture. 7: 273-282.
24-Kafi M., and Stowart, D. 2001. The effects of salinity on growth and yield of nine cultivar of wheat. Journal of Agriculture Science and Technology. Vol 12. No 1.
25-Kaya, C., Higgs, D., and Kirnak, H. 2001. The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach. Bulgarian Journal Plant physiology. 27: 47-59.
26-Khoshgoftar, A., and Siadat, H. 2001. Mineral nutrition of vegetables and horticultural crops in salt condition. Publishing and agricultural training center.Karaj. Iran. 87 pp.
27-Marschner, H. 1995. Mineral nutrition of higher plants. Academic Press. London. 889 pp.
28-Meybodi, S., Ghareyazi, B. 2002. Physiological and breeding aspects in salt stress in plants. Isfahan University Press.
29-Munns, R., and Tester, M. 2008. Mechanisms of salinity tolerance. Annual Review Plant Biology. 59: 651-681.
30-Najafi, F., Khavari-Nejad, R. A., and Siah Ali, M. 2010. The effects of salt stress on physiological parameters in summer savory (Satureja hortensis L.) plant. Journal Stress Physiology Biochemistry. 6(1): 14-21.
31-Santa-Maria, G. E., and Epstein, E. 2001. Potassium/sodium selectivity in wheat and amphiploid cross wheat x Lophopyrum elongatum. Plant Science. 160: 523-534.
32-Schachtman, D., and Munns, R. 2002. Sodium accumulation in leaves of Triticum species that differ in salt tolerance. Australian Journal Plant physiology. 19(3):21,331-340.
33-Shen W, Nada, K., and Tachibana, S. 2000. Involvement of polyamines in the chilling tolerance of cucumber cultivars. Plant Physiology. 124: 431-439.
34-Spahn, C., Blaha, G., Stelzel, U., Agrawal, RK., Frank, J., and Nierhause, KH. 2000. Preparation of functional ribosomal complexes and effect of buffer conditions on tRNA positions observed by cry electron microscopy. Methods Enzymol. 317: 292-309.
35-Sultana, N., Ikeda, T. and Itoh, R. 1999. Effec of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environmental and Experimental Botany. 42: 211-220.
36-Tester, M., and Davenport, R. 2003. Na+ tolerance and Na+ transport in higher plants. Annals Botany. 91: 503-527.
37-Weimberg R, Lerner, HR., and Poljakoff-Mayber, A. 1982. A relationship between potassium and proline accumulation in salt-stressed Sorghum bicolor. Physiology Plantarum. 55: 5-10.
38-Wittenmayer, L., and Merbach, W. 2005. Plant responses to drought and phosphorus deficiency: Contribution of phytohormones in root-related processes. Journal Plant Nutrition Soil Science. 168(4): 531-540.
39-Zuccarini, P. 2008. Effect of silicon on photosynthesis, water relations and nutrient uptake of Phaseolus vulgaris under NaCl stress, Biology Plantarum, 52(1): 157-160.