Effect of Different Culture Media on the Micropropagation of GF677 (Prunusamygdalus ×P. persica)

Document Type : Research Article

Authors

1 Ferdowsi University of Mashhad

2 Agricultural Biotechnology Research Institute of Karaj

3 Zanjan University

Abstract

Introduction: The GF677(Prunusamygdalus×P. persica) is a peach rootstock tolerant to Fe deficiency. Nowadays, it is mainly propagated through micro propagation. Widening and undesirable growth of leaves as well as poor rooting are major problems during its in vitro culture. GF-677 is one of the most suitable rootstocks for almond and peach used in calcareous soils to overcome lime-induced chlorosis. Therefore, in vitro micro propagation is important for commercial purposes. Using liquid medium, it may be possible to reduce costs to a level lower than solid medium and liquid medium is better than solid medium in growth. Both the brand and concentration of agar also affect the chemical and physical characteristics of a culture medium. One of the main factors on micropropagation is hormone specially BAP. Furthermore, shoot branching depends on the initiation and activity of axillary meristems, which usually controlled by cytokinin. The rooting stage, the induction of roots on explants from in vitro culture is crucial part in any micropropagation process. The ability of plant tissue to form adventitious roots depends on interaction of many exogenous and endogenous factors, including hormone. Most reports of adventitious root induction of woody species have involved treatments with exogenous auxins such as IBA, NAA or IAA. Dimassi-Theriou (1995) for rooting of GF-677 compared different culture media and results on the rooting of these rootstocks depend on the type of medium culture.
Materials and Methods: Axillary shoot of GF677 was cultured on both liquid and solid media. In proliferation step both liquid and solid media (MS, DKW and WPM) were used in primary stages of the experiment. Medium containing BAP 1mg.land-1 NAA 0.1mg.l-1. Under growth chamber conditions, light intensity was maintained at 2500-3000 lux with an 8-hour dark period. For rooting, 3-4 cm-long shoots from previous culture were transferred to 1/2 MS medium containing IBA (0, 0.5, 1 and 1/5mg.l-1) and 6 , 0 g l-1 agar. Darkness during the last week of the rooting phase has been shown to be necessary in stimulating rooting in some woody species. Note that the room temperature was maintained at 25°C during this experimental stage. The experiment was carried out based on factorial adopted completely randomized design with 5 replications per treatment. Explants shoot lengths, shoot numbers, root lengths and root numbers were recorded after 4 weeks which propagated plants via tissue culture were transferred to soil medium using 50% peat and 50% perlit mixture.
Results and Discussion:
Shoot proliferation: The observation indicates that there were significant differences between solid and liquid media. Best results were achieved for proliferation by liquid medium and among which MS obtained the highest frequency. The highest number of shoot was observed in MS medium and the lowest number of shoot was observed in WPM medium. Increasing mineral concentration resulted in increased multiplication, growth rate and total mineral uptake by GF677 explants.
Root initiation of in vitro: Various concentrations of IBA showed significant differences. The maximum number of roots and root length were observed in the medium containing 0.5 mg.l-1 IBA. The best results were obtained for rooting in liquid 1/2 MS supplemented with 0.5 mg.l-1 IBA. The mean survival of the plants were transferred to liquid medium (75%) and mean survival of the plants were transferred from the solid culture medium (50%).
Conclusion: In conclusion, a micropropagation system for GF677 has been worked out utilizing nodal explants. Our investigation showed that the liquid MS medium with 1 mg.lit-1 BAP was the best for proliferation of GF677 and micropropagated plants were rooted and established in soil successfully. WPM medium is higher in chloride level which has been reported to result in growth depression in plants due to inhibited nutrient uptake, transport and utilization of nutrients variation in multiplication and growth of explants can be explained on the basis of water potential and mineral availability to the explants in the liquid medium. Many investigators have reported that IBA has a better effect on promoting adventitious root formation in comparison to IAA. The best results were obtained for rooting in 1/2 MS supplemented with 0.5 mg. l/1 IBA.

Keywords


1- Afreen F. 2007.Temporary immersion bioreactor. Plant Tissue Culture Engineering, 6: 87–201.
2- Alvard D., Cote F., and TeissonC. 1993. Comparison of methods of liquid medium culture for bananapropagation.Effects of temporary immersion of explants. Plant Cell, Tissue ad Organ Culture., 32: 55-60.
3- Amiri M.E. 2002. Mass Propagation of a Unique Variety of Pear (PyruspyrifoliaNak.Cv. Sebri) by shoot Tip Culture in vitro.ActaHorticulturae, 587:55-56.
4- Andreu P., and Marin J. A. 2005.In vitro culture establishment and multiplication oftheprunus rootstock Adesoto 101 (P. insititiaL.) as affected by the type of propagation of the donor plant and by the culture medium composition.ScientiaHorticulturae, 106: 258-267.
5- Ainsley P.J., Collins G.G., and Sedgley M. 2001. In vitro rooting of almond (prunusdulcis mill.). In Vitro Cellular & Developmental Biology,37, 778–785.
6- BattistiniA., and Paoli G. 2002. Large scale micropropagation of several peach rootstocks. ActaHorticulturae, 592: 29-33.
7- Debergh P. C. 1987. Effects of agar brand and concentration on the tissue culture medium.Physiologia Plantarum, 59(2): 270–276.
8- Debergh PC, Aitken-Christic J, Cohen B, Von Arnold S, Zimmerman R., and Ziv, M. 1992. Reconsideration of the term "vitrification" as used inmicropropagation. Plant Cell, Tissue and Organ Culture, 30: 135-140
10- Dimasi-Theriou K., and Economou A. S. 1995. Ethylene enhances shoot formation in cultures of the peach rootstock GF-677 (Prunuspersica x P. amygdalus). Plant Cell Reports,15: 87–90.
11- Dobranszki J., and Teixeira da Silva J.A. (2010). Micropropagation of apple – A review.Biotechnology Advances, 28: 462-488.
12- Driver J.A., and Kunyoxi H. 1984. In Vitro propagation of Paradox Walnut rootstocks. Horticultural Science, 19:507-509.
13- Etienne H., and Berthouly M. 2002. Temporary immersion systems in plant micropropagation. Plant Cell, Tissue and Organ Culture, 69: 215-231.
14-Fasolo F., Malavasi F., and Ranieri R. 1987.Preliminary investigation on in vivo rooting of micropropagation of GF-677, peach rootstock.ActaHorticulturae, 212: 181-287.
15-Hartmann HT., Kester DE.,and Davies FT. 1990.Plant propagation, Principles and practices. Prentice-Hall International, New Jersey, USA. Pp. 145-89.
16-Kamali K., Majidi E., and Zarghami R. 2006.Micropropagation of GF677 rootstocks (Prunusamygdalus× P. persica).Plant Genetics and Breeding, 56: 175- 177.
17-Lioyd, G.B., and B.H. Mc Crown. 1980; Commerically feasible micro propagation of mountain by use of shoot tip culture. Proc. Inter. Plant propoter. Sco.30:421-437.
18-Majidi E., and Davodi D. 2005. Microtuber production in potato by periodical bioreactor. Journal 0f Agronomy Science of Iran, 5(4): 302-304.
19- Molassiotis A., Dimassi N., Therios K., and Diamantidis I. 2003. Fe-EDDHA promotes rooting of rootstock GF677 (Prunusamygdalus×P.persica) explants invitro. BiologiaPlantarum, 47 (1): 141-144.
20- Murashige T., and Skooge F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue culture. Physiology Plant,15: 473-497.
21-Rossel G., De Bertoldi G., and TizzioR, 1987.In vitro mass tuberisation as a contributiontopotatomicropropagation. Potato Research, 30: 111-116.
22-Ruzic D., Saric M., Cerovic R., and Culafic L. 2003. Contents of macroelements
and growth of sweet cherry rootstock in vitro. Biology of Plants, 47: 463-465.
23-SinghaS. 1982. Influence of agar concentration on in vitro shoot proliferation of Malussp.Almey and PyruscommunisSeckel. Journal of American Society of Horticultural Science, 107: 657--660.
24-Sandal I, Bhattacharya A., and Ahuja PS. 2001.An efficient liquid culturesystem for tea shoot proliferation. Plant Cell, Tissue and Organ Culture, 65:75-80.
25-Smith M.A.L., Spomer L.A. 1994.Vessels, gels, liquid media and supportsystems. In: Aiktken-Christie J, Kosai T, Smith MAL (eds.). (1994).Automation and Environmental control in plant tissue culture. KluwerAcademic Publishers, Dordrecht, 371-404.
26-Tabachnik L., and Kester D.E. 1977. Shoot culture for almond and almond peach hybrid clones invitro. Horticultural Science, 12(6): 545-547.
27- Tatarivernosafadarani M, Mousavi S.A., and buzarim N. 2012. Micropropagation of some Clonal Root stocks of Stone Fruits. Seed and plant Improvement Journal. 1-28(1): 53-66 (in Persian).
28-Ziv M. 1989.Enhanced shoot and cornlet proliferation in liquid cultured gladiolus buds by growth retardants.Plant Cell, Tissue and Organ Culture, 17: 101-110.
29-Ziv M. 1991. Vitrification: morphological and physiological disorders of in vitro plants. In:Debergh,P.C. and Zimmerman, R.H. Micropropagation. Kluwer Academic Publishers, Dordrecht,The Netherlands; pp. 45-69.
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