بهینه‌سازی عوامل موثر در ازدیاد درون شیشه‌ای پایه میروبالان c29

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 مرکز تحقیقات کشاورزی و منابع طبیعی خراسان رضوی

چکیده

این مطالعه با هدف بررسی اثر محیط کشت و تنظیم کننده‌های رشد بر شاخه‌زایی، ریشه‌زایی و سازگاری میروبالانC29 انجام شد. در این مطالعه از آزمایش فاکتوریل بر پایه طرح کاملا تصادفی با 4 تکرار و هر تکرار شامل 5 نمونه استفاده شد. به منظور ضد عفونی نمونه های گیاهی از 2 تیمار، شامل اتانول 70 درصد در 1 دقیقه و هیپوکلریت سدیم 10 درصد در 10 دقیقه استفاده شد. نتایج نشان داد هیپوکلریت سدیم 10 درصد به مدت 10 دقیقه، با 4 درصد آلودگی ریز نمونه های سال جاری بهترین تیمار ضدعفونی بود. از دو نوع محیط کشت شامل MS و DKW و تنظیم کننده رشد بنزیل آمینوپورین در غلظت های 0، 1، 2، 3 و 4 میلی گرم در لیتر در مرحله پرآوری و ایندول بوتیریک اسید در غلظت های 0، 1، 2 و3 میلی گرم در لیتر در مرحله ریشه زایی استفاده شد. نتایج نشان داد که محیط کشت MS حاوی 2 میلی گرم در لیتر بنزیل آمینو پورین با میانگین پر آوری 16/6 شاخساره، و طول 51/2 سانتی متر بیش ترین شاخه زایی و بیش ترین درصد ریشه زایی در محیط MS حاوی 2 میلی گرم در لیتر IBA و طول ریشه به میزان 100 درصد و 75/6 سانتی متر، به دست آمد. سازگاری گیاهچه ها به شرایط گلخانه ای با استفاده از سیستم مه افشانی، با موفقیت انجام شد بالاترین درصد بقای گیاهچه (0/70 درصد) در محیط کوکوپیت و پرلایت (1:1 حجمی) به دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Optimization of Factors Affecting in vitro Propagation of ‘Myrobalan 29c’ Rootstock

نویسندگان [English]

  • zahra shabani 1
  • Bahram Abedi 1
  • Abrahim Gangimoghadam 2
  • Ali Tehranifar 1
1 Ferdowsi University of Mashhad
2 KhorasanRazavi Research Center for Agriculture and Natural Resource
چکیده [English]

Introduction: This study was conducted aimed to consider the effects of culture medium and the concentration of growth regulators on proliferation, rooting and the acclimatization of Myrobalan29C. This study was performed as a factorial experiment in a completely randomized design (CRD) with four replications where each plot contained five explants. Given the role of trees rootstock of growth rate vegetative in the early maturity٫ yield and disease resistance will be suitable rootstock that has an important role in program garden management. In total rootstock of fruit trees have be propagation sexual and asexual methods, however٫ given that in sexual reproduction the resulting dispersion characteristics and the resulting seedlings changed by genetics, it is tried for decades to asexual propagation methods in specially tissue culture methods healthy rootstock for mass propagation and used the development of orchards. Seedling rootstock Myrobalan had been used in the past. The rootstock can be positive features cited the ease of access, to be cheap, good yields after maturity. In study, the effects of NAA and BAP on proliferation of Gisala5 rootstock showed the most shoot treated in mediacontaining 1mg/l BAP. Investigated Chinese plum in vitro micropropagation showed that 1/2 MS media had the highest percentage rooting, and acclimatization rooted plantlets to greenhouse conditions was using the system miss successfully. Due to the importance and essential to achieve an efficient protocol for the mass propagation of Myrobalan 29C in Iran, this study was conducted with the main purpose of evaluating the most suitable media culture and plant growth regulators in micropropagation of Myrobalan 29C.
Material and methods: The explants were collected from shoots of Myrobalan 29C rootstock maintained in the experimental greenhouse of Khorasan Razavi Agricultural and Natural Resources Research Centre (of Mashhad, Iran), on June 25, 2013. The explants were washed by water and dishwashing liquid to removed surface contamination. Then they were divided to some parts containing one bud and were sterilized with ethanol 70% 1 min and sodium hypochlorite 10% at 10 min. Proliferation was performed in two kind of culture medium (MS and DKW) that supplemented with plant growth regulators BAP (0, 1, 2, 3, 4 mg l-1). The rootstocks of in this step, after subculture three (21 days between each subculture), the numbers, the length and quality of the shoots (explants strong growth, with no signs of vitrification, necrosis of leaf are yellowing terminal meristem), b– less than 15% have the symptoms of vitrification, necrosis of leaf are yellowing terminal meristem, and c– explant weak, 15-30% have the symptoms of vitrification, necrosis of leaf are yellowing Terminal meristem) were measured. This stage was carried with four replications and each replicates with five samples. Two culture media (MS and DKW) were used for rooting, which supplemented with indole-3-butyric acid (IBA) at four levels (0, 1, 2, 3 mg l-1). This stage was carried with four replications and each replicates with three samples. After being rooted explants, the best cultured media and combination of rooting growth regulators number and root length, leaf number and stem length and quality of explants were recorded. Acclimatization used in substrate including Coco Peat - Perlite 3:0/5 V, Coco Peat 100%V٫ Coco Peat - Perlite 0.5:3V, Perlite100%V and Coco Peat - Perlite1:1V).
Results and Discussions: Results showed that the 10% sodium hypochlorite for 10 min, with 4% decay was the best treatment for sterilization. The results showed that the proliferation average was 6/16 in MS medium with 2 mgl-1 BAP and the most percent of rooting and root length were about 100% and 2/51 cm in MS medium with 2mgl-1 of IBA, respectively. The acclimatization of plantlets to greenhouse conditions was successful. The highest rate of plantlets survival (about 70%) was obtained from substrate Cocopit and Prlit (1; 1 V). In the present study, explants year compared annual explant least contamination had enjoyed. It seems that the young explants, smooth the surface of the skin, having the least amount of crack depend on the type of surface depressions explants and the crack and lower depressions the surface explants increase surface contact area disinfectants and also improve its impact. In this study found that the type of medium a significant impacted on the health of plants and so the proliferation of explants was successful. Usually root production in plants under the influence of synthesis, metabolism, and transport is auxin signaling pathways. Therefore acclimatization directly affected the rooting of plants that had high quality and the best rate of induction.
Conclusions: The results of this research showed that we can duplicate Myrobalan29c rootstock by in vitro method. According in this research, MS media including BAP and IBA plant growth regulators are the most suitable for micro propagation.

کلیدواژه‌ها [English]

  • Acclimatization; Disinfection
  • Rooting and Proliferation
1- Asadi A. A., Vedadi C., Rahimi M., and Naserian B. 2009. Effect of plant growth hormones on root and shoot regeneration in rose (Morrasia) under in-vitro conditions, Bioscience Research, 6(1): 40-45.
2- Azadi P., Khosh- Khui M., Beyramizadeh E., and Bagheri H. 2007. Optimization of factors affecting in vitro proliferation and rooting of Rosa hybrida L. cv. 'Rafaela', International Journal of Agricultural Research, 2(7): 626-631.
3- Channuntapipat Ch., Sedgley M., and Collins G. 2003. Micropropagation of almond cultivars Nonpariel and Ne Plus Ultra and the hybrid rootstock Titan × Nemagard. Sci. Hort. 98:473-484.
4- Damiano.C., Delia G. 2009. In vitro multiplication, rooting acclimatization and related protein profiles of rootstock citation (Prunus.Salicina × prunus. Persica). Acta Horticulturas, 812: 218-227.
5- Hartmann H.T., Kester D.E., and Davies F.T. 1990. Plant propagation, principles and practices. Prentice-Hall International, New Jersey, USA, Pp, 145-89.
6- Ganji Moghadam, A., and Abdollahzadeh A. 2009. Guide fruit trees rootstock (Translate). Khorasan Razavi Agricultural and Natural Resources Research Center٫S155-184. (Edited book).
7- Gudin S. 2000. Rose: genetics and breeding. Plant Breeding Review, 17: 59-189.
8- George E. F. 2008. Plant propagation by tissue culture. Plant Cellular Reproduction, 18: 6-113.
9-Izad Panah. 2004. The effects of age and genotype on theway propagation Sweet Cherry (Prunus avium) in vitro.Natural Sources٫64:63-70. (in Persian)
10-Kamali K., Majidi A., and Zarghami R. 2002. Determine the most suitable culture medium and growth conditions Micropropagationof vegitative GF677 (hyberid Paech×Almound) rootstock. Journal and seed, 17:38-47. (in Persian)
11- Larson E. L., and Morton H. E. 1991. Disinfection, Sterilization and Preservationon, In Alcohols.
12-Mahdavyan M., Bozari N., and Abdollahi H. 2010. Effect of media and plant growth regulators on proliferation and rooting Mahaleb (Sent losi 64) of vegitative rootstock .Journal and seed٫ 1:15-26. (in Persian)
13- Movsiuw A. 2011. In vitro propagation of virus-free Myrobalan 29c rootstock. GRI, 7349:101-102.
14- Ruzic D. V., Vajovic. T. I., 2013. The effect of cytoknins types and their concentration on in vitro multiplication of sweet cherry. Horticulture Scienc, 35: 12-21.
15- Sana B., Ghosh D., Saha M., and Mukherjee J. 2006. Purification and characterization of a salt, solvent, detergent and bleach tolerant protease from a new gamma- Proteobacterium isolated from the marine environment of the Sundarbans. Process Biochemistry, 41(1): 208-215.
16- Soleymanpor A., and Bozari N. 2012. Effect of media and plant growth regulators in micro propagation Mahaleb (SL- 64) of vegitative rootstock.Especially a genetic Congress Iran, 12:1-5.9. (in Persian)
17- Pati P. K., Rath S. P., Sharma M., and Ahuja P. S. 2005. Micropropagation, protoplast cu lture and its implications in the improvement of scented rose. Acta Horticulturae, 547: 147-158.
18- Plopa C., Dutu N., Isac V., Mazilu C., Ancu S. 2010. Factors Influencinrg in Vitro Propagation of Myrobolan Dwarf Plum Root stock. ISHS Acta Horticulturae, 966:221-223.
19- Perez-Tornero O., Opez J.M.L. 2000. Effect of basal media and growth regulators on the in vitro propagation of apricot (prunus armenica L.) cv. Cannino, j. Hort. ci, Biotech, 75: 283-286.
20- Pruski K., Astatkle T. 2005. Tissue culture propagation of monogolian cherry (prunus froticosa) and nanking cherry (Prunus tomentosa). Plant Cellular Tissue and Organ Culture, 82: 207-211.
21- Yadollahi A., Nazari Moghadam Aghaee R., and Moeeni A. 2009.Effect composition BAP and NAA on prolifiration Gisela rootstock. Especially a genetic Congress Iran, 3:1-5. (in Persian)
22- Yari F., and Mosavi A. 2012. Optimization in vitro five varieties of cut roses (Roza hybrida L.). Biotechnology in agriculture٫ 10: 17-26.
23- Ying-Ning Z. 2010. Micropropagation of Chinese Plum (Prunus salicina Lindl.) Using Mature Stem Segments. Not. Bot. Hort. Agrobot. Cluj, 38 (3): 214-218.
24- Zlesak D. 2006. Rose. In Flower Breeding and Genetics, 695-740 (Ed N. Anderson). Springer Netherlands.
25-Zolfaghari Nasab R., Khosro shahi M., Gerigoryan V., and Matlabi azar A. 2005. The effects in vitro propagation natural hybrid Apricot×plum. Journal of Horticultural Science and Technology, 5: 81-92 (in Persian)