بررسی تغییرات فیزیولوژیکی و بیوشیمیایی پسته رقم احمدآقایی روی ژنوتیپ‌های پایه متحمل و حساس به سرما در شرایط تنش یخ‌زدگی

نوع مقاله : مقالات پژوهشی

نویسندگان

1 سازمان تحقیقات، آموزش و ترویج کشاورزی، رفسنجان

2 دانشگاه تهران

3 دانشگاه شهید باهنر کرمان

4 ایستگاه تحقیقات پسته دامغان

چکیده

به‌منظور بررسی اثر ژنوتیپ پایه بر واکنش پسته رقم احمدآقایی نسبت به تنش یخ‌زدگی، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار انجام شد. تیمارها در دو سطح: ژنوتیپ پایه (چهار ژنوتیپ متحمل و چهار ژنوتیپ حساس به سرما) و دما (2- و 4- درجه سانتی‌گراد) اجرا شد. در مرحله تمام گل، از شاخه‌های انتهایی رقم احمد آقایی پیوند شده روی این ژنوتیپ‌ها نمونهگیری شد. شاخه‌ها در گلدان‌های حاوی آب مقطر در دمای 2- و 4- درجه سانتی‌گراد به مدت دو ساعت قرار گرفتند. پس از اعمال تیمارها، شاخص سرمازدگی تعیین شد، ازخوشه‌های گل تیمار‌ها جهت اندازه‌گیری پارامترهای فیزیولوژیکی و بیوشیمیایی استفاده گردید. نتایج نشان داد شدت سرمازدگی در ژنوتیپ‌های پایه متحمل به سرما به‌طور معنی‌داری کمتر از ژنوتیپ‌های پایه حساس در دو دمای 2- و 4- درجه سانتی‌گراد می‌باشد. میزان نشت یونی، پراکسید‌هیدروژن، مالون‌دآلدهید در ژنوتیپ‌های پایه متحمل به سرما به‌طور معنی‌داری پایین‌تر از ژنوتیپ‌های حساس به سرما بود. میزان قندهای محلول، پروتئین کل، پرولین و فعالیت آنزیم‌های‌کاتالاز، آسکوربات‌پراکسیداز و گایاکول‌پراکسیداز در ژنوتیپ‌های پایه متحمل به سرما به‌طور معنی‌داری بیشتر از ژنوتیپ‌های پایه حساس به سرما بوده است. نتایج مربوط به اثر متقابل دما و ژنوتیپ نشان داد که دمای 4- درجه سانتی‌گراد باعث افزایش معنی‌دار نشت یونی، پرولین، پراکسید‌هیدروژن و مالون‌دآلدهید و کاهش فعالیت آنزیم‌های کاتالاز، آسکوربات‌پراکسیداز و گایاکول‌پراکسیداز نسبت به دمای 2- درجه سانتی‌گراد مخصوصا در پایه‌های حساس به سرما شد. با توجه به صفات مورد مطالعه، متحمل‌ترین ژنوتیپ‌ پایه به سرما TR1 بود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Physiological and Biochemical Changes of Pistachio (Pistacia vera L. cv. Ahmad-Aghaii) on Cold Tolerant and Sensitive Rootstocks under Freezing Stress Conditions

نویسندگان [English]

  • Ali Tajabadipour 1
  • Mohammadreza Fattahi Moghadam 2
  • Zabihollah Zamani 2
  • Fatemeh Nasibi 3
  • Hossein Hokmabadi 4
1 Agricultural Research,Education and Extension Organization (AREEO)
2 University of Tehran
3 University of Shahid Bahonar, Kerman
4 Agricultural and Natural Resources Research Center of Semnan Province (Shahrood), AREEO
چکیده [English]

Introduction: Spring cold injury is one of the main limiting factors to production and distribution of pistachio. Pistachio is one of the most valuable and exported agricultural crops of Iran. Since, spring frosts results to considerabe damage to this plant, hence, it is important to investigate methods for reducing freezing damage. For this reason, selection of rootstocks and cultivars are an important objective in breeding programs. Freezing temperatures (below 0ºC) cause the movement of water from the protoplast to the extracellular space, resulting in the growth of extracellular ice crystals and ultimately, cell dehydration. Plants have developed complex processes to survive and recover from unfavorable conditions. To tolerate cold stresses, plants develop multiple mechanisms, including the accumulation of cryoprotective molecules and proteins, alterations in membrane lipid composition, and primary and secondary metabolite composition, as well as changes in global gene and protein expression Frost affects cell membranes, which become less permeable, and even break, giving rise to the leakage of solute from damaged cells. There is often a good correlation between ion leakage and freezing tolerance (22). Sugars may depress the freezing point of the tissue and act as a nutrient and energy reserve, alter phase properties of membranes in the dry state and act as cryoprotectants to preserve protein structure and function. Other compounds acting similarly are lipids, soluble proteins and free proline (44). Proline seems to have diverse roles under osmotic stress conditions, such as stabilization of proteins, membranes and subcellular structures and protecting cellular functions by scavenging reactive oxygen species (23). The aim of the present study was to evaluate different degrees of sensitivity to low temperatures in different genotypes and ‘Ahmad-Aghaii’ cultivar in relation to physiological and biochemical changes in field conditions.
Materials and Methods: In order to determine the effects of rootstock on pistachio cultivar ‘Ahmad-Aghaii’ under freezing stress conditions, an experiment was carried out as factorial based on a randomized completely design (RCD) with four replications. Treatments consisted of two levels: 1- rootstock genotype (four cold sensitive and tolerant rootstocks) and 2- temperatures (-2 and -4 ºC). The sampling was performed in full bloom stage from apical branches of pistachio cultivar ‘Ahmad-Aghaii’ budded on these rootstock genotypes. The branches in pots contain distillted water treated under -2 and -4 °C for 2 h. After treatment, the chilling index was determined. Flower clusters were used for measuring physiological and biochemical parameters. All determinations were carried out in four triplicates and data were subjected to analysis of variance. Analysis of variance was performed using the ANOVA procedure. Statistical analyses were performed according to the SAS software. Significant differences between means were determined by Duncan’s multiple range tests. P values less than 0.05 were considered statistically significant.
Results and Discussion: The results showed that chilling index was significantly lower in the cold-tolerant rootstocks than cold-sensitive rootstocks at -2 and -4 ºC. Also, Results indicated that electrolyte leakage, hydrogen peroxide (H2O2) and malondialdehyde (MDA) were significantly lower in tolerant rootstocks than sensitive ones. The content of soluble carbohydrate, total protein and proline were significantly higher intolerant rootstocks than sensitive ones. The activity of anti-oxidant enzymes ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) in tolerant rootstocks was greater than sensitive rootstocks. The reaction of temperature and rootstock indicated that electronic leakage, proline, H2O2,و and MDA significantly increased in -4 ºC. The activity of anti-oxidant enzymes APX, GPX and CAT decreased in -4 ºC as compared to -2 °C especially in cold-sensitive rootstocks. Some researchers believe that the accumulation of proline is as an index to select the drought-resistance varieties (26, 48). Stated that there was no comprehensive information about the relationship between the accumulation of proline and tension resistance. Research on apricot and peach confirmed the results of the present study because this pattern is also seen in their proline level (26 and 41). While the starch concentration decreases during the dormancy, the amount of proline increases which is in accordance with their results (36).
Conclusions: In this study, the damage of the membrane increased with decreasing temperature. The results showed that the rootstocks could increase the resistance to cold by increasing the amount of soluble sugars, protein, proline and the activity of the antioxidant system in the shoots and leaves of the scion. Regarding physiological and biochemical studies, it was determined that ‘Ahmad-Aghaii’ cultivar budded on cold tolerant rootstocks had higher soluble sugars, total protein, proline and CAT, APX and GPX enzymes activity and had less chilling index, ion leakage, H2O2 and MDA, which indicates less damage to the membrane of the cell and its contents compared with the cultivar 'Ahmad-Aghaii' budding to sensitive rootstocks. Consequently, the findings of this study selected TR1 as the most tolerant rootstock compared to other ones.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • Cold tolerance
  • Pistachio
  • Rootstock
1- Abedi B., Tafazol A., Rahimi M., Khaldabrin B., and Ganji A. 2010. Changes in sugars, starch, proline and inter-tissue water in cold weather in some cultivars of apricot (Prunus armeniaca L.). Journal of Horticultural Science, 4: 382-375. (In Persian with English abstract)
2- Afshar Mohammadian M., Rezaie S.h., and Ramezani Malekrudi M. 2012. Investigating the resistance of two olive cultivars to cold stress. Journal of Process and Plant Function, 2: 11-1. (In Persian with English abstract)
3- Afshari H., Hokmabadi H., Ebadi A., and Laee G. 2010. Measurement of chemical and non-chemical parameters of three native pistachio cultivars of damghan region (Iran) for studying spring frost. Asian Journal of Chemistry, 22(3): 2356.
4- Alexieva V., Sergiev I., Mapelli S., and Karanov E. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell and Environment, 24(12): 1337-1344.
5- Arab H., Hakamabadi H., and Tajbadipour A. 2014. Investigation of cold resistance in four pistachio rootstocks through ion leakage parameter and macroscopic observations. Graduate Student, Islamic Azad University, Jiroft Branch. (In Persian with English abstract)
6- Azzarello E., Mugnai S., Pandolfi C., Masi E., Marone E., and Mancuso S. 2009. Comparing image (fractal analysis) and electrochemical (impedance spectroscopy and electrolyte leakage) techniques for the assessment of the freezing tolerance in olive. Trees, 23: 159-167.
7- Bakht J., Bano A., Shafi M., and Dominy P. 2013. Effect of abscisic acid applications on cold tolerance in chickpea (Cicer arietinum L.). European Journal of Agronomy, 44: 10-21.
8- Barranco D., Ruiz N., and Gomez-del Campo M. 2005. Frost tolerance of eight olive cultivars. HortScience, 40(3): 558-560.
9- Bartolini S., Leccese A., Iacona C., Andreini L., and Viti R. 2014. Influence of rootstock on fruit entity, quality and antioxidant properties of fresh apricots (cv. ‘Pisana’). New Zealand Journal of Crop and Horticultural Science, 42(4): 265-274.
10- Bates L.S. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207.
11- Bradford M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254.
12- Brend A., Kalantari N., Nasibi F., and Rezandeh F. 2012. Physiological effects of arginine amino acid pre-treatment on induction of cold resistance in pistachio plant Pistacia vera under glassy conditions. Graduate Student of Shahid Bahonar University of Kerman. 99 pages. (In Persian with English abstract)
13- Carbonell‐Barrachina Á.A., Memmi H., Noguera‐Artiaga L., Gijon‐Lopez M.D.C., Ciapa R., and Perez‐Lopez D. 2015. Quality attributes of pistachio nuts as affected by rootstock and deficit irrigation. Journal of the Science of Food and Agriculture, 95(14): 2866-2873.
14- Chen B., Huang J., Wang J., and Huang L. 2008. Ultrasound effects on the antioxidative defense systems of Porphyridium cruentum. Colloids and Surfaces B: Biointerfaces, 61(1): 88-92.
15- Cohen S., and Naor A. 2002. The effect of three rootstocks on water use, canopy con-ductance and hydraulic parameters of apple trees and predicting canopy from hydraulic conductances. Plant, Cell and Environment, 25: 17–28.
16- Davey M.W., Stals E., Panis B., Keulemans J., and Swennen R.I. 2005. High throughput of malondialdehyde in plant. Analytical Biochemistry, 347: 201-207.
17- Dhindsa R.S., Dhindsa P., and Thorpe A.T. 1981. Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decrease levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32: 93-101.
18- Emmert F.H., and Howlett F.S. 1953. Electrolytic determinations of the resistance of fifty-five apple varieties to low temperatures. Proceedings of the American Society for Horticultural Science, 62: 311-318.
19- Ferguson L., and Buchner R. 1990. Relative cold tolerance of four unbudded pistachio seedling rootstocks. HortScience, 25(9): 1089-1089.
20- Ferguson L., Reyes H., Sanden B., Grattan S., Epstein L., and Krueger .2005. Pistachio rootstocks, in Pistachio Production Manual, ed. by Ferguson L Center for Fruit and Nut Research and Information, Davis, CA, pp. 67-73.
21- Gijon M.D., Gimenez C., Perez-Lopez D., Guerrero J., Couceiro J.F., and Moriana A. 2010. Rootstock influences the response of pistachio {Pistacia vera L. cv. Kerman) to water stress and rehydration. Scientia Horticulturae (Amsterdam), 125: 666-671.
22- Gill S.S., and Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48: 909-930.
23- Guy C.L. 1990. Cold acclimation and freezing stress tolerance: Role of protein metabolism. Annual Reviews in Plant Physiology and Plant Molecular Biology, 41: 187-223.
24- Heath R.L., and Packer L. 1968. Photoperoxidation in isolated chloroplast, kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125 (1): 189-198.
25- Hokmabadi H. 2016. Investigation of cold resistance in three commercial cultivars of pistachio Damghan and three major pistachio cultivars through ion leakage parameters. The final report of the research project of the Pistachio Research Institute of IRAN. 68 pages. (In Persian with English abstract)
26- Holt B.C. 2003. Substances which inhibit ice nucleation cryletters. Horticultural Reviews, 13: 215-237.
27- Honty K., Sardi É., Stefanovits-Banyai É., and Toth M. 2008. Frost induced changes in enzyme activities and carbohydrate content in the spurs of some pear cultivars during the dormancy. International Journal Horticultural Science, 14: 41-44.
28- Imani A., Barzegar K., and Piripireivatlou S. 2011. Relationship between frost injury and ion leakage as an indicator of cold hardiness in 60 almond selections. International Journal of Nuts and Related Sciences, 2(1): 22-26.
29- Irigoyen J.J., Einerich D.W., and Sanchez‐Diaz M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativsa) plants. Physiologia Plantarum, 84(1): 55-60.
30- Khorshidi M., Nojavan A.M. 2006. The effects of abscisic Acid and CaCl2 on the activities of anti-oxidant enzymes under cold stress in maize seedlings in the dark. Journal of Biological Sciences, 9: 54-59.
31- Kim S.Y., Lim J.H., Park M.R., Kim Y.J., Park T.I., Seo Y. W., and Yun S.J. 2005. Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. BMB Reports, 38(2): 218-224.
32- Li B., Liu L.Q., Luo S.P., Li N., Li J., Cheng M.L., and Li L. 2012. Effects of low temperature stree on flower bud cold resistance of almonds. Journal of Xinjiang Agricultural University, 1: 002.
33- Liu B., Li M., Cheng L., Liang D., Zou Y., and Ma F. 2012. Influence of rootstock on antioxidant system in leaves and roots of young apple trees in response to drought stress. Plant Growth Regulation, 67(3): 247.
34- Lu J.X., Jiang H.Y., and Li W. 2012. Effects of low temperature stress on the cold resistance of rootstock and branch of wine grapes. Journal of Fruit Science, 29(6): 1040-1046.
35- Lučić B., Jovanović Ž., Radović S., and Maksimović V. 2009. Cold-induced response of buckwheat (Fagopyrum esculentum Moench) seedlings. Archives of Biological Sciences, 61(3): 3-4.
36- Lukatkin A.S. 2002. Contribution of oxidative stress to the development of cold-induced damage to leaves of chilling-sensitive plants: 2. the activity of antioxidant enzymes during plant chilling. Russian Journal of Plant Physiology, 49(6): 782-788.
37- Mansouri Dehsheebi R., Davari Nejad G.h., Hokmabadi H., and Tehranifar Z. 2011. Evaluation of changes in proline, total protein and soluble sugars during phonological stages of flower buds of Pistachio cultivars. Journal of Horticulture (Agricultural Science and Technology), 25(2): 121-116. (In Persian with English abstract)
38- Mohacsy M., Maliga P., and Mohacsy M. 1959. The peach. Agriculture Publisher, Budapest. 397 p.
39- Nadernejad N., Ahmadimoghadam A., Hossyinifard J., and Poorseyedi S. 2013. Effect of different rootstocks on PAL activity and phenolic compounds in flowers, leaves, hulls and kernels of three pistachio (Pistacia vera L.) cultivars. Trees, 27(6): 1681-1689.
40- Nakano Y., and Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach choloroplast. Plant and Cell Physiology, 22: 867-880.
41- Palonen P., Buszard D., and Donnelly D. 2000. Changes in carbohydrates and freezing tolerance during cold acclimation of red raspberry cultivars grown in vitro and in vivo. Physiologia Plantarum, 110: 393–401.
42- Perveen S., Anis M., and Aref I.M. 2013. Lipid peroxidation, H2O2 content, and antioxidants during acclimatization of Abrus precatorius to exvitro conditions. Biologia Plantarum, 57(3): 417-424.
43- Plewa M.J., Smith S.R., and Wagner E.D. 1991. Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Res, 247: 57-64.
44- Rodrigo J. 2000. Spring frost in deciduous fruit trees morphological damage and flower hardiness. Scintia Horticulture, 85: 155-173.
45- Salary Sorkhan R., Enteshari S., Hokmabadi H., and Tajabadipour A. 2011. Physiological evaluation of pistachio frost damage resistant rootstocks. International Journal of Nuts and Related Sciences, 2 (4): 55-66.
46- Sohrabi N., Hakam Abadi H., and Tajabadipour A. 2009. Freezing physiology in pistachio prees, Extension issue, Pistachio Research Institute of Iran. 35 p. (In Persian with English abstract)
47- Solari L., Johnson S., and Dejong T.M. 2006. Hydraulic conductance characteristics of peach (Prunus persica) trees on different rootstocks are related to biomass production and distribution. Tree Physiology, 26: 1343–1350.
48- Szabados L., and Savoure A. 2009. Proline: a multifunctional amino acid. Trends in Plant Science, 15 (2): 89-97.
49- Vitamvas P., and Prašil I.T. 2008. WCS120 protein family and frost tolerance during cold acclimation, deacclimation and reacclimation of winter wheat. Plant Physiology and Biochemistry, 46(11): 970-976.
50- Wang W.B., Kim Y.H., Lee H.S., Yong Kim K., Deng X., and Wak S.K. 2009. Analysis of antioxidant enzymes activity during germination of alfalfa under salt and drought stresses. Journal of Plant Physiology and Biochemistry, 47(7): 570-577.
51- Weibel A., Johnson R.S., and DeJong T.M. 2003. Comparative vegetative growth responses of two peach cultivars grown on size-controlling versus standard rootstocks. Journal of the American Society for Horticultural Science, 128: 463–471
52- Xu S-C., Yong-Ping L., Hu J., Ya-Jing G., Yun-ye Z., and Shui-jin Z. 2010. Responses of antioxidant enzymes to chilling stress in tobacco seedlings. Agricultural Sciences in China, 9(11): 1594-1601.