Evaluation of the Effect of Exogenous Application of Polyamines on Growth, Nut Traits and Yield of ‘Akbari’ Pistachio Trees (Pistacia vera L.)

Document Type : Research Article

Authors

• Gh.H. Davarynejad ¹
• M. Nurzadehnamaghi ²
• A. Momen ³

¹ Professor Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

² Ph.D. Gratuated (Pomology Tendency), Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

³ Ph.D. Agriculture (Agroecology tendency), Agronomy Department, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

10.22067/jhorts4.v34i4.75961

Abstract

Introduction: Pistachio (Pistacia vera L.) is an important horticultural crop that has high economic value. Pistachio trees display some physiological disorders including abscission of inflorescence buds, fruit abscission, blankness, non-splitting, early splitting and deformation of nuts. Unfavorable environmental conditions in most pistachio orchards and inadequate nutrition during the flowering time and after flowering has increased these problems in recent years. Polyamines such as putrescine, spermine and spermidine are some low molecular weight polycationic compounds that have been shown to play an important role as growth regulators in different stages of growth and development of buds, flowers and fruits and resistant plants to abiotic and biotic stress. Therefore, the objective of this study was to investigate the influence of polyamines in water scarcity condition on growth, nut traits and yield of ‘Akbari’ pistachio trees.
Materials and Methods: The study was carried out in growing season 2016 in a pistachio orchard (Pistacia vera L. cv Akbari) located in Feyzabad, Mahvelat, Khorasan Province, Iran (34° 40´ N latitude, 58° 25´ E longitude, and around 1253 m above sea level). The climate of the experimental area was arid and semi-arid. The mean annual precipitation was about 171 mm. The irrigation of orchard trees was at 36-day intervals. The experiment was conducted as split plot based on a randomized complete block design with three replications and seven treatments including polyamines spray of Putrescine (Put), Spermine (Spm) and Spermidine (Spd) at concentrations of zero (control), 0.1 and 1 mM on “on” and “off” pistachio trees cultivar Akbari at two-time stages full bloom (FB) and two weeks after full bloom (2WAFB). Leaf chlorophyll index was recorded using the chlorophyll meter (SPAD-502, Konica Minolta, Japan). Leaf area from fully expanded leaves was measured by Digital Leaf Area Meter (LI-3100c). The length and diameter of current-year shoots were measured at harvest time. The percentage of flower bud abscission and fruit abscission were calculated on marked shoots. The yield was calculated by weighting the total dried split nuts separated from each shoot. The data were subjected to analysis of variances (ANOVA) using the SAS software (SAS version 9.1). Difference between means was compared using the least significant difference test (LSD) at 5% level (p ≤ 0.05).
Results and Discussion: Results showed that among the treatments, Put (0.1 and 1 mM) and Spm (0.1 mM) applied in the “on” trees significantly increased leaf chlorophyll index. Also, Put spray at two weeks after full bloom indicated better results in increase of length shoot in ‘on’ trees. All of the polyamines reduced fruit abscission in the “on” trees however, Put at 0.1 and 1 mM concentrations with 11.3 and 11.1% was more effective in decreasing the percentage of fruit abscission compared to other treatments. Fruit abscission of pistachio is assumed to be related to improper fertilization, the dominance of fruits in a cluster or abnormalities of reproductive organs. Polyamines have been suggested to improve fertilization, and subsequent embryo and fruit development. It also has been reported that common precursor of S-adenosylmethionine (SAM) needed for synthesizing both of PAs and ethylene. Thus, it seems that polyamines reduce the abscission of fruits by the improvement of embryo growth and development or by antagonism with ethylene, possibly by competing for S-adenosylmethionine (SAM). Our study indicated that all of the polyamines decreased the percentage of blank nuts in the “off” trees especially two weeks after full bloom. The results showed that Put (0.1 and 1 mM) and Spd (0.1 mM) were very effective in increasing number of nuts per cluster.  Number of nuts per 100 g, split nuts, and kernel percentage did not differ significantly among treatments. Yield variations were not significantly affected by polyamines in in the “off” trees, but in ‘on’ trees, polyamines of Put, Spm and Spd at 0.1 mM concentration by 46.2, 14.6 and 15.2% and at 1 mM concentration by 38.7, 24.7 and 19.9% increased yield compared to control, respectively. Given to correlation results, the positive effect of polyamines on yield improvement in ‘on’ trees was mainly due to the prevention from fruit abscission and enhancement no. of nuts per cluster.
Conclusion: Results showed that application of polyamines treatments in ‘off’ trees only on blank nuts and in ‘on’ trees on leaf chlorophyll index, fruit abscission, No. of nuts per cluster and yield was effected. Foliar application of polyamines was more effective in improving traits of shoot length, leaf area and blank nuts two weeks after full bloom compared to full bloom. The results of this study showed that these plant growth regulators, especially putrescine, had the potential to improve growth, yield and reduce physiological problems of pistachio under unfavorable environmental conditions.

Keywords

• Inflorescence bud abscission
• Fruit abscission
• Putrescine
• Spermidine
• Spermine

20.1001.1.20084730.1399.34.4.2.1

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References

1-         Acar I., and Eti S. 2007. Abscission of pistachio flowers and fruits as affected by different pollinators. Pakistan Journal of Biological Science 10: 2920-2924.

2-         Ahmad P., Kumar A., Gupta A., Hu X., ul Rehman Hakeem K.H., Mahgoub Azooz M., and Sharma S. 2012. Polyamines: Role in Plants under Abiotic Stress. p. 491-512. M. Ashraf et al. (eds.) Crop Production for Agricultural Improvement. Springer Science+Business Media B.V. 2012.

3-         Alcázar R., Marco F., Cuevas J.C., Patron M., Ferrando A., Carrasco P., Tiburcio A.F., and Altabella T. 2006. Involvement of polyamines in plant response to abiotic stress. Biotechnology Letters 28: 1867–1876.

4-         Anjum M.A. 2011. Effect of exogenously applied spermidine on growth and physiology of citrus rootstock Troyer citrange under saline conditions. Turkish Journal of Agriculture and Forestry 35: 43-53.

5-         Arias M., Carbonell J., and Agusti M. 2005. Endogenous free polyamines and their role in fruit set of low and high parthenocarpic ability citrus cultivars. Journal of Plant Physiology 162: 845–853.

6-         Aziz A., Brunb O., and Audran J. 2001. Involvement of polyamines in the control of fruitlet physiological abscission in grapevine (Vitis vinifera). Physiologia Plantarum 113: 50–58.

7-         Bagheri S., Rahemi M., Abedy B., Nemati H., and Rowshan V. 2017. Changes in polyamine levels in relationship to the growth and development of parthenocarpic fruits (shotberries) of olive (Olea europaea L.). Scientia Horticulturae 215: 172-177.

8-         Bouchereau A., Aziz A., Larher F., and Martin-Tanguy J. 1999. Polyamines and environmental challenges: recent development. Plant Science 140: 103-125.

9-         Chapman S.C., and Barreto H.J. 1997. Using chlorophyll meter to estimate specific leaf nitrogen of tropical maize during vegetative growth, Agronomy Journal 89(4): 557-562.

10-     Crisosto C.H., Lombard B.P., Sugal D., and Polito V.S. 1988. Putrescine influences ovule senescence, fertilization time, and fruit set in ‘Comice’ pear. Journal of the American Society for Horticultural Science 113(5): 708-712.

11-     FAO. 2008. Production Year Book.Vol. 62. F.A.O. Rome, Italy

12-     FAO. 2017. http://www.fao.org/faostat/en/#data/QC.

13-     Ferguson L., Beede R.H., Freeman M.W., Haviland D.R., Holtz B.A., and Kallsen C.E. 2005. Pistachio Production Manual (4^th ed.). Fruit and Nut Research and Information Center, University of California, Davis, California.

14-     Fritschi F.B., and Ray J.D. 2007. Soybean leaf nitrogen, chlorophyll content, and chlorophyll a/b ratio. Photosynthetica 45: 92- 98.

15-     Galston A.W., and Kaur-Sawhney R. 1990. Polyamines in plant physiology. Plant Physiology 94: 406-410.

16-     Galston A.W., Kaur-Sawhney R., Altabella T., and Tiburcio A.F. 1997. Plant polyamines in reproductive activity and response to abiotic stress. Botanica Acta 110: 197–207.

17-     Groppa M.D., Rosales E.P., Iannone M.F., and Benavides M.P. 2008. Nitric oxide, polyamines and Cd-induced phytotoxicity in wheat roots. Phytochemistry 69: 2609–2615.

18-     Kamiab F., Heidari Salehabad M., and Zamanibahramabadi E. 2015. Evaluation the effects of foliar treatments of polyamines and some organic acids on quantitative and qualitative traits in some pistachio cultivars. Journal of Nuts 6(2): 131-142.

19-     Kamiab F., Talaie A., Khezri M., and Javanshah A. 2013. Exogenous application of free polyamines enhances salt tolerance of pistachio (Pistacia vera L.). Plant Growth Regulation 72: 257-278.

20-     Khezri M., Talaie A., Javanshah A., and Hadavi F. 2010. Effect of exogenous application of free polyamines on physiological disorders and yield of ‘Kaleh-Ghoochi’ pistachio shoots (Pistacia vera L.). Scientia Horticulturae 125: 270-276.

21-     Kumar A., Altabella T., Taylor M., and Tiburcio A.F. 1997. Recent advances in polyamine research. Trends in Plant Science 2: 124–130.    

22-     Kusano T., Berberich T., Tateda C., and Takahashi Y. 2008. Polyamines: essential factors for growth and survival. Planta 228: 367–381.

23-     Kushad M.M. 1998. Changes in polyamine levels in relationship to the double-sigmoidal growth curve of peaches. Journal of the American Society for Horticultural Science 123: 950–955.

24-     Liu H.P., Dong B.H., Zhang Y.Y., Liu Z.P., and Liu Y.L. 2010. Relationship between osmotic stress and the levels of free, conjugated and bound polyamines in leaves of wheat seedlings. Plant Science 166: 1261–1267.

25-     Lopatin A.N., Makhina E.N., and Nichols C.G. 1994. Potassium channel block by cytoplasmic polyamines as the mechanism of intrinsic rectification. Nature 372: 366–369.

26-     Malik A., and Singh Z. 2006. Improved fruit retention, yield and fruit quality in mango with exogenous application of polyamines. Scientia Horticulturae 110: 167–174.

27-     Malik A.U., and Singh Z. 2004. Endogenous free polyamines of mangos in relation to development and ripening. Journal of the American Society for Horticultural Science 129: 280–286.

28-     Malmberg R.L., Watson M.B., Galloway G.L., and Yu W. 1998. Molecular genetic analyses of plant polyamines. Critical Reviews in Plant Sciences 17: 199–224.

29-     Minotti P.L., Halseth D.E., and Sieckza J.B. 1994. Field chlorophyll measurements to assess the nitrogen status of potato varieties. American Society for horticultural Science 29(12): 1497-1500.

30-     Pistocchi R., Bagni N., and Creus J.A. 2002. Polyamine uptake in carrot cell cultures. Plant Physiology 84: 374-380.

31-     Pritsa T.S., and Voyiatzis D.G. 2004. Seasonal changes in polyamine content of vegetative and reproductive olive organ in relation to floral initiation, anthesis and fruit development. Australian Journal of Agricultural Research 55: 1039-1046.

32-     Roussos P.A., Pontikis C.A., and Zoti M.A. 2004. The role of free polyamines in the alternate-bearing of pistachio (Pistacia vera cv. Pontikis). Trees 18: 61–69.

33-     Shiozaki S., Ogata T., and Horiuchi S. 2000. Endogenous polyamines in the pericarp and seed of the grape berry during development and ripening. Scientia Horticulturae 83: 33-41.

34-     Shuraki Y.D., and Sedgley M. 1996. Fruit development of Pistacia vera (Anacardiaceae) in relation to embryo abortion and abnormalities at maturity. Australian Journal of Botany 44: 35–45.

35-     Slocum R.D., and Galston A.W. 1985. Changes in polyamine biosynthesis associated with postfertilization growth and development in tobacco ovary tissues. Plant Physiology 78: 323-8.

36-     Stern R.A., and Gazit S. 2000. Application of the polyamine putrescine increased yield of ‘Mauritius’ litchi (Litchi chinensis Sonn.). Journal of Horticultural Science and Biotechnology 75: 612–614.

37-     Talaie A., Khezri M., and Javanshah A. 2010. Effect of exogenous application of free polyamines on physiological disorders of ‘Kaleh-Ghoochi’ pistachio (Pistacia vera L.). Journal of Iranian Horticultural Science 4: 383-391. (In Persian)

38-     Thompson M. 1996. Flowering, pollination and fruit set. p. 223–241. In Webster A.D., Looney N.E. (ed.) Cherries: Crop Physiology, Production and Uses. CAB Intl, UK.

39-     Tiburcio A.F., Campos J.L., Figueras X., and Besford R.T. 2002. Recent advances in the understanding of polyamine functions during plant development. Plant Growth Regulator, 12: 331-340.

40-     Toumi I., Moschou P.N., Paschalidis K.A., Bouamama B., Salem-fnayou A.B., Ghorbel A.W., Mliki A., and Roubelakis–Angelakis K.A. 2010. Abscisic acid signals reorientation of polyamine metabolism to orchestrate stress responses via the polyamine exodus pathway in grapevine. Journal of Plant Physiology 167: 519-525.

41-     Ullah Malik A., and Singh S. 2007. Improved fruit retention, yield and fruit quality in mango with exogenous application of polyamines. Scientia Horticulturae 110: 167–174.

42-     Westwood M.N. 1997. Temperate-Zone Pomology: Physiology and Culture. Freeman W.H., and Co, San Francisco, pp. 428.

43-     Ziosi V., Scaramagli S., Bregoli A.M., Biondi S., and Torrigiani P. 2003. Peach (Prunus persica L.) fruit growth and ripening: transcript levels and activity of polyamine biosynthetic enzymes in the mesocarp. Journal of Plant Physiology 160: 1109–1115.