Journal of Horticultural Science
with the collaboration of Iranian Scientific Association for Landscape (ISAL)

Study Flowering and Effect of Various Hand Pollination Methods on Fertility of some Native Salvia Species of Iran

Document Type : Research Article

Authors

Gh. Esmaeili 1
M. Azizi 2
H. Arouiee 2
J. Vaezi 3

1 1- Royesh Yakhte Neko Co (Royanak), Mahhalat City, Markazi Province, Iran 2- Department of Horticulture Science and Landscape Architecture, Faculty of Agriculture, Ferdowsi University of Mashhad,Mashhad, Iran

2 Department of Horticulture Science and Landscape Architecture, Faculty of Agriculture, Ferdowsi University of Mashhad,Mashhad, Iran

3 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

https://doi.org/10.22067/jhs.2021.61510.0
Abstract
Introduction
The genus Salvia L. (Lamiaceae) is one of the most interesting plant genera with a variety of annual, biennial, perennial and shrub forms. According to recent reports, there are 61 species of Salvia genus in Iran, among which 18 species are endemic. The use of Salvia species dates back to ancient times, and they are now being used for different purposes such as antimicrobial, anthelmintic, anti-inflammatory, tonic, carminative, digestive, Alzheimer’s disease, antispasmodic as well as for traditional medicines and food industries. Compared to crop plants, the breeding programs of medicinal plants are still in nascent stages due to high biological and biochemical variations. Since breeding programs, especially in medicinal plants, are expensive, suitable mating can help reduce the costs of breeding programs. Understanding the flowering biology, reproductive systems and pollination mechanism is essential to management and protection of ecosystems and mating programs. Despite the high diversity of Salvia genus in Iran, there is a paucity of studies on reproductive biology.
 
Materials and Methods
 seeds of seven Salvia species including S. virgata (with four accessions), S. frigida, S. nemorosa, S. atropatana, S. macrosiphon, S. sclarea, and S. syriaca were collected from different geographical regions. The seeds were treated by various methods such as washing, cold treatment (stratification), scarification (sand and sulfuric acid), Gibberellic acid (GA3) and KNO3 to enhance germination. The seedlings were produced at the greenhouse and after growing as high as 15 cm or reaching the10-leaf stage, the seedlings were transferred to the farm. Plants phenology and flowering duration were recorded in the second year. Furthermore, some flower characteristics and pollinator were monitored. The Various pollination treatments were designed to investigate the breeding system of Salvia species including open pollination, spontaneous autogamy, geitonogamy, xenogamy, apomixis, and control. The self-incompatibility index (ISI) was calculated for each species in different pollination treatment.
 
Results and Discussion
The germination study exhibited that washing seeds for 24 h and applying 250 ppm GA3 exerted the highest effect on seed germination. The seedlings in early growth stages required medium to high porosity (high permeability to water and air) like natural habitat, but they were sensitive to dry and high EC at these stages. The phenology studies were shown Salvia virgata (all accessions) and S. nemorosa flowered three months after cultivation and other species flowered in the second year. The results showed that all species had long flowering periods ranging from 15 (S. atropatana) to 41 days (S. virgata). Increased flowering period led to the enhancement of flower over-lapping and the sharing of the pollen among different plants or species. Two species including S. atropatana and S. syriaca had heterostyly flowers (long-styled morph). The staminal-lever-mechanism was observed in all species. The results of manual pollination indicated that open pollination provided the best treatment with the highest average of seed set (73.63%) in all species. Salvia virgata (A1) with a seed set of 90.3% was the best species, and S. syriaca with a seed set of 51.5% demonstrated the poorest performance in open pollination system. The seed set in the xenogamy treatment of different species and accessions was in the range of 10.2 (S. syriaca) to 32.5% (S. frigida). It was observed that seed set among different species in geitonogamy and spontaneous autogamy varied widely from 2.60 - 17.30% and 2.98 - 12.18%, respectively. In treatments with a wide variety of pollen sources, the fruit set was higher, indicating that the pollen limitation triggered low seed set.
 
Conclusion
Salvia species in the present study have a high potential for cultivation as medicinal and ornamental due to suitable adaptability and long flowering period. Salvia species were relatively self-incompatible that intention to the out-crossing and breeders can use in breeding programs as a valuable characteristic. The self-incompatible and specific structure in flower and pollination are the reasons for the diversity of this taxa.
 

Keywords

Flower biology
Heterostyly
Open pollination
Self-incompatibility
Subjects

Authors retain the copyright. This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0)

  1. Bahadori, M.B., Dinparast, L., Zengin, G., Sarikurkcu, C., Bahadori, S., Asghari, B., & Movahhedin, N. (2017). Functional components, antidiabetic, anti-Alzheimer’s disease, and antioxidant activities of Salvia syriaca International Journal of Food Properties, 20(8), 1761–1772. https://doi.org/10.1080/10942912.2016.1218893
  2. Barrett, S.C.H., Wilken, D.H., & Cole, W.W. (2000). Heterostyly in the Lamiaceae: The case of Salvia brandegeei. Plant Systematics and Evolution, 223(3–4), 211–219. https://doi.org/10.1007/BF00985280
  3. Campos, C.C., Duarte, J.F., Tristão, R.A., & Borém, D.M.C. (2009). Floral biology and breeding mechanisms of Tibouchina heteromalla Cogn. in rocky outcrops in the south of Minas Gerais. Brazilian Journal of Ecology, 8(2), 85–97.
  4. Castro, S., Silveira, P., & Navarro, L. (2008a). How flower biology and breeding system affect the reproductive success of the narrow endemic Polygala vayredae Costa (Polygalaceae). Botanical Journal of the Linnean Society, 157(1), 67–81. https://doi.org/10.1111/j.1095-8339.2008.00784.x
  5. Castro, , Silveira, P., & Navarro, L. (2008b). Effect of pollination on floral longevity and costs of delaying fertilization in the out-crossing Polygala vayredae Costa (Polygalaceae). Annals of Botany, 102(6), 1043–1048. https://doi.org/10.1093/aob/mcn184
  6. Celep, F., Atalay, Z., Dikmen, F., Doğan, M., & Classen-Bockhoff, R. (2014). Flies as pollinators of melittophilous Salvia species (Lamiaceae). American Journal of Botany, 101(12), 2148–2159. https://doi.org/10.3732/ajb.1400422
  7. Charlesworth, D. (2006). Evolution of Plant Breeding Systems. Current Biology, 16(17), R726–R735. https://doi.org/10.1016/j.cub.2006.07.068
  8. Claßen-Bockhoff, R., Wester, P., & Tweraser, E. (2003). The staminal lever mechanism in Salvia (Lamiaceae) - A review. Plant Biology, 5(1), 33–41. https://doi.org/10.1055/s-2003-37973
  9. Claßen-Bockhoff, R., Speck, T., Tweraser, E., Wester, P., Thimm, S., & Reith, M. (2004). The staminal lever mechanism in Salvia (Lamiaceae): A key innovation for adaptive radiation? Organisms Diversity and Evolution, 4(3), 189–205. https://doi.org/10.1016/j.ode.2004.01.004
  10. Cursach, , & Rita, J. (2012). Reproductive biology of Ranunculus weyleri (Ranunculaceae), a narrowly endemic plant from the Balearic Islands with disjunct populations. Flora - Morphology, Distribution, Functional Ecology of Plants, 207(10): 726–735. https://doi.org/10.1016/j.flora.2012.07.004
  11. Dafni, A. (1992). Pollination Ecology: A Practical Approach. IRL Press at Oxford University Press, UK, 250 pp.
  12. Dudash, M.R., & Fenster, C.B. (2001). The role of breeding system and inbreeding depression in the maintenance of an outcrossing mating strategy in Silene virginica (Caryophyllaceae). American Journal of Botany, 88(11), 1953–1959. https://doi.org/10.2307/3558422
  13. Evans, M.E.K., Menges, E.S., & Gordon, D.R. (2003). Reproductive biology of three sympatric endangered plants endemic to Florida scrub. Biological Conservation, 111(2), 235–246. https://doi.org/10.1016/S0006-3207(02)00293-8
  14. Gan, X., Cao, L., Zhang, X., & Li, H. (2013). Floral biology, breeding system and pollination ecology of an endangered tree Tetracentron sinense (Trochodendraceae). Botanical Studies, 54(1), 50.  https://doi.org/10.1186/1999-3110-54-50
  15. Gaudeul, M., & Till-Bottraud, I. (2004). Reproductive ecology of the endangered alpine species Eryngium alpinum (Apiaceae): Phenology, gene dispersal and reproductive success. Annals of Botany, 93(6), 711–721. https://doi.org/10.1093/aob/mch098
  16. Harder, L.D., & Johnson, S.D. (2009). Darwin’s beautiful contrivances: Evolutionary and functional evidence for floral adaptation. New Phytologist, 183(3), 530–545. https://doi.org/10.1111/j.1469-8137.2009.02914.x
  17. Jamzad, Z. (2013). A survey of Lamiaceae in the flora of Iran. Rostaniha, 14(1), 59–67. https://doi.org/22092/botany.2013.101317
  18. Jorge, A., Loureiro, J., & Castro, S. (2015). Flower biology and breeding system of Salvia sclareoides (Lamiaceae). Plant Systematics and Evolution, 301(5), 1485–1497. https://doi.org/10.1007/s00606-014-1169-7
  19. Kalisz, S., & Vogler, D.W. (2003). Benefits of autonomous selfing under unpredictable pollinator environments. Ecology, 84(11), 2928–2942. https://doi.org/10.1890/02-0519
  20. Miyajima, D. (1996). Seed-producing system in Salvias. Journal of the American Society for Horticultural Science, 121(3), 419–422. https://doi.org/21273/JASHS.121.3.419
  21. Miyajima, D. (2001). Floral variation and its effect on self-pollination in Salvia splendens. Journal of Horticultural Science and Biotechnology, 76(2), 187–194. https://doi.org/1080/14620316.2001.11511349
  22. Miyake, Y.C., & Sakai, S. (2005). Effects of number of flowers per raceme and number of racemes per plant on bumblebee visits and female reproductive success in Salvia nipponica (Labiatae). Ecological Research, 20(4), 395–403. https://doi.org/10.1007/s11284-004-0035-4
  23. Nautiyal, B.P., Nautiyal, M.C., Khanduri, V.P., & Rawat, N. (2009). Floral biology of Aconitum heterophyllum: A critically endangered alpine medicinal plant of Himalaya, India. Turkish Journal of Botany, 33(1), 13–20. https://doi.org/10.3906/bot-0805-8
  24. Navarro, L. (1997). Is the dichogamy of Salvia verbenaca (Lamiaceae) an effective barrier to self-fertilization? Plant Systematics and Evolution, 207(1–2), 111–117. https://doi.org/10.1007/BF00985212
  25. Nebot, A., Cogoni, D., Fenu, G., & Bacchetta, G. (2016). Floral biology and breeding system of the narrow endemic Dianthus morisianus Vals. (Caryophyllaceae). Flora, 219, 1–7. https://doi.org/10.1016/j.flora.2015.12.004
  26. Ott, D., Hühn, P., & Claßen-Bockhoff, R. (2016). Salvia apiana — A carpenter bee flower? Flora: Morphology, Distribution, Functional Ecology of Plants, Flora, 221, 82-91. https://doi.org/10.1016/j.flora.2015.12.008
  27. Pannell, J.R., & Barrett, S.C.H. (1998). Baker’s Law Revisited: Reproductive Assurance in a Metapopulation. Source: Evolution, 52(3), 657–668. https://doi.org/10.1111/j.1558-5646.1998.tb03691.x
  28. Qu, L., & Widrlechner, M.P. (2011). Variation in the breeding system of prunella variation in the breeding system. Hortscience. HortScience, 46(5), 688-692. https://doi.org/21273/HORTSCI.46.5.688
  29. Rodríguez-Pérez, J. (2005). Breeding system, flower visitors and seedling survival of two endangered species of Helianthemum (Cistaceae). Annals of Botany, 95(7), 1229–1236.  https://doi.org/1093/aob/mci137
  30. Sanchez, L.A., Picado, A., Sommeijer, M.J., & Slaa, E.J. (2002). Floral biology, pollination ecology and seed production of the ornamental plant Salvia splendens Journal of Horticultural Science and Biotechnology, 77(4), 498–501. https://doi.org/10.1080/14620316.2002.11511529
  31. Şenol, G., Eroğlu1, V., Şentürk, O., Kaçmaz, F., & Avcl, A.B. (2017). The pollination and reproduction success of Salvia sclarea Serdar. Biological Diversity and Conservation, 3, 130–135.
  32. Silva, F.O., Da Viana, B.F., & Jacobi, C.M. (2005). Floral biology of Eriope blanchetii (Lamiaceae) in coastal sand dunes of NE Brazil. Austral Ecology, 30(3), 243–249. https://doi.org/10.1111/j.1442-9993.2005.01443.x
  33. Stöbbe, J., Schramme, J., & Claßen-Bockhoff, R. (2016). Training experiments with Bombus terrestris and Apis mellifera on artificial ‘Salvia’flowers. Flora, 221, 92-99. https://doi.org/10.1016/j.flora.2015.12.007
  34. Subaşi, Ü., & Güvensen, A. (2011). Breeding systems and reproductive success on Salvia smyrnaea. Turkish Journal of Botany, 35, 681–687. https://doi.org/10.3906/bot-1012-105
  35. Takano, A. (2013). Gynodioecy in Salvia omerocalyx Hayata (Lamiaceae). APG: Acta Phytotaxonomica et Geobotanica, 63(3), 149–153. https://doi.org/18942/apg.kj00008609663
  36. Wester, P., & Claßen-Bockhoff, R. (2006). Hummingbird pollination in Salvia haenkei (Lamiaceae) lacking the typical lever mechanism. Plant Systematics and Evolution, 257(3–4), 133–146. https://doi.org/10.1007/s00606-005-0366-9
  37. Wester, P., & Claßen-Bockhoff, R. (2007). Floral diversity and pollen transfer mechanisms in bird-pollinated Salvia Annals of Botany. Oxford University Press. pp. 401–421.
  38. Will, M., Schmalz, N., & Classen-Bockhoff, R. (2015). Towards a new classification of Salvial.: (re)establishing the genus Pleudia Raf. Turkish Journal of Botany, 39(4), 693–707. https://doi.org/10.3906/bot-1405-34
  39. Will, M., & Claßen-Bockhoff, R. (2017). Time to split Salvial. (Lamiaceae) – New insights from Old World Salvia phylogeny. Molecular Phylogenetics and Evolution, 109, 33–58. https://doi.org/10.1016/j.ympev.2016.12.041
  40. Wright, S.I., Kalisz, S., & Slotte, T. (2013). Evolutionary consequences of self-fertilization in plants. Proceedings of the Royal Society B. Biological Sciences, 280(1760), 20130133. https://doi.org/1098/rspb.2013.0133
  41. ZhenQiao, S., JianHua, W., HongGang, W., FuJuan, Z., & LiWu, H. (2009). Studies of the floral biology, breeding characters of Salvia miltiorrhiza. Acta Horticulturae Sinica, 36, 905–910.

 

 

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  • Receive Date 14 May 2019
  • Revise Date 15 February 2023
  • Accept Date 10 March 2021
  • First Publish Date 10 March 2021

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