Document Type : Research Article


1 Department of Horticulture, Faculty of Agricultural Technology, University of Tehran,Tehran, Iran

2 Department of Horticultural Science, Aburaihan Campus, University of Tehran, Tehran, Iran

3 Department of Agronomy and Plant Breeding, Aburaihan Campus, University of Tehran, Tehran, Iran


 Cantaloupes, are a group of Cucumis melo cultivars, that have round, fragrant fruits with netted skin. Our country, is one of the top five producers of cantaloupe, and melon in the world. Its original origin, is still debated, but one of the important centers of its diversity, is Iran. Although, native cultivars, such as, Saveh cantaloupe, Samsoori, Rish Baba, and various types of Tiles, are often of good quality, and taste, but they are sensitive to a variety of fungal and viral diseases and as a result, their yield is low. On the other hand, every year new cultivars and hybrids, are introduced by seed companies, which are welcomed by farmers, due to, their better agronomic characteristics, and resistance to a wider range of diseases. Continuation of this process, in addition to severe genetic erosion of native cultivars, and populations, and even the complete elimination of some of them, will have consequences such as, dependence of production on foreign companies, annual departure of currency from the country, and non-exploitation of the country's genetic resources. Therefore, it seemed that by creating a diverse population resulting from the crossing of superior native cultivars with cultivars of commercially resistant hybrid to diseases, and then, successive selections, new cultivars, with desirable traits of both parents, could be achieved. Thus, in this study, heritability, minimum and maximum mean values of parents, and F1 and F2 generations, aggressive segregation, and relative frequency of qualitative traits, in F2 generation, and selection of the best genotypes, in two dispersing NGF2 populations, were investigated.
Materials and Methods
 Parental cross, was performed in the spring of 2017, between Samsoori cantaloupe cultivar (round, striped fruit, cream skin color, completely reticulate, green flesh, very early ripening fruit, poor transportability and durability, free pollinator and high homogeneity) as the selected native paternal parent, and commercial cultivar from Gallia group, that called Cory and as maternal parent (round fruit, completely reticulate, no striped skin, yellow to cream skin color, high transportability and durability, green and very sweet flesh, high resistance to various plant diseases and viral diseases) and produced by Seminis Company. The first-generation seeds, obtained from parental crosses, were planted in the greenhouse in the fall of 2017, and were self-sown. In the spring of 1397, 1000 seeds of F2 generation, along with parent seeds, and their first generation, were planted in a seedling tray, in the greenhouses of the Faculty of Agricultural Technology (Aborihan) of Tehran University, and transplanted to the ground, in the rental research production farm, located, in Filistan village-Golzar sector-Pakdasht. Controlled pollination, (isolation of male flowers, and manual inoculation) was performed, for all second-generation plants. Selection for subsequent generations, was done by pedigree method, all plants in F2 generation, were evaluated, and selected. Morphological traits, such as, plant form and phenological traits, such as, day to fruit harvest, were evaluated, and recorded. Plant health, was assessed against common and important fungal, viral and mites, under normal field conditions by scoring the severity of infections in four categories. Fruit quality traits were measured based on nominal, sequential and interval scales and quantitative fruit traits were also measured. Tables of minimum and maximum values, variances, general heritability, transgressive segregation, etc for quantitative traits, also relative frequency percentages and observed genetic ratios for quantitative traits, were calculated.
Results and Discussion
 The results showed that all quantitative traits in the F2 population had a general heritability above 90%. The mean of all quantitative traits except soluble solids and fruit flesh thickness in F1 population was lower than the parental average. In F2 population, for all quantitative traits, positive transgressive segregation was observed, compared to, the superior parent, and negative transgressive segregation was observed, compared to, the less valuable parent. In the F2 population, nearly 80% of the genotypes, were completely reticulate, and 20% had less or no netting, on the fruit skin. These results were almost consistent with genetic ratios of 3.1. Also, 35% of F2 population genotypes, were striped and more than 80% of them had yellowish skin color and green flesh color. Due to, the fact that, selection based on traits with high heritability, will be more reliable, and successful in early generations, so, it is better, to select the best genotypes, in this population, based on fruit weight, soluble solids, and early maturity, respectively.
In the F2 population, positive transgressive segregation was evident for all quantitative traits, favoring the superior parent, while negative segregation was observed for the less valuable parent. Traits exhibiting high heritability showcased a pronounced influence of genetic variance over environmental variance. Consequently, selecting cultivars based on these traits in early generations is expected to yield more reliable and successful outcomes. Therefore, it is better, to selection, the best genotypes in the early generations, in the F2 population, based on fruit weight, (with high genetic efficiency), soluble solids, day to fruit ripening, and with the pedigree management, of the populations, it is finally, possible to achieve lines, that have the desirable traits, of both parental cultivars. The resulting lines, can be used to produce, new hybrids, or provided, to the farmer, as a single cultivar.


Main Subjects

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  1. References

    1. Adib, F.Y., Wiko, A.W., & Budi, S.D. (2022). Genetic stability of melon (Cucumis melo cv. Meloni) based on inter-simple sequence repeat and phenotypic characteristics. Biodiversitas, 23(6), 3042-3049.
    2. Anam, R., Shailesh, M., & Vijay, B. (2022). DUS Characterization in snap melon landraces of vindhayan region of estern U. P. Indian as per Muskmelon on PPV and FRA guidelines. Plant Archives, 22(2), 322-326.
    3. Choudhary, B.R., Pandey, S., Rao, E.S., & Sharma, S.K. (2015). DUS characterization of muskmelon (Cucumis melo) varieties. Indian Journal of Agricultural Sciences, 85(12), 1597–1601.
    4. Clayberg C.D. (1992). Interaction and linkage tests of flesh color genes in Cucumis melo Cucurbit Genetics Cooperative Report, 15, 53.
    5. Cuevas, H.E., Staub, J.E., Simon, P.W., & Zalapa, J.E. (2009). A consensus linkage map identifies genomic regions controlling fruit maturity and beta-carotene-associated flesh color in melon (Cucumis melo). TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik119(4), 741–756.
    6. Dal, Y., Kayak, N., Kal, Ü., Seymen, M., & Türkmen, Ö. (2016). Yerel kavun (Cucumis melo) Genotiplerinin bazı morfolojik Özellikleri.11. Sebze Tarımı Sempozyumu Ordu Üniversitesi.
    7. Damicone, J., Shrefler, J., & Brandenberger, L. (2020). Guide for identification and management of diseases of cucurbit vegetable crops. ‏Oklahoma Cooperative Extension Service. Division of Agricultural Sciences and Natural Resources Oklahoma State University, E-929,
    8. Danin-Poleg, Y., Tadmor, Y., Tzuri, G., Reis, N., Hirschberg, J., & Katzir, N. (2002). Construction of a genetic map of melon with molecular markers and horticultural traits, and localization of genes associated with ZYMV resistance. Euphytica, 125, 373-384.
    9. Dorri, P., Khavari-Khorasani, S., Valizadeh, M., & Taheri, P. (2014). The study of inheritance and gene effects on yield and agronomic traits of early generations of genetic maize Dehghan (KSC400). Plant Genetics Research, 1(2), 33-42. (In Persian with English abstract).
    10. Eduardo, I., Arus, P., & Van der Knaap, E. (2007). Estimating the genetic architecture of fruit quality traits in melon using a genomic library of near isogenic lines. Journal of the American Society for Horticultural Science, 132, 80-89.
    11. Ehdaie, B. (2008). Plant breeding. University of Tehran Press, p.589.
    12. A.O. (2016). Biodiversity: Agricultural biodiversity in FAO. From:
    13. A.O. 2019. FAOSTAT agricultural database. Available at:
    14. Feyzian, E., Dehghani, H. Rezai, A., & Jalali Javaran, M. (2009b). Correlation and sequential path model for some yiel related traits in Melon (Cucumis melo ). Iranian Journal of Agricultural Sciences, 11, 341-353.

    1. Feyzian, E., Dehghani, H., Rezai, A. M., & Jalali Javaran, M. (2009a). Diallel cross analysis for maturity and yiel related traits in melon (Cucumis melo L.). Euphytica, 168 (2), 215-223. DOI:1007/s10681-009-9904-9
    2. Fita, A., Pico, B., Monforte, A. J., & Nuez, F. (2008). Genetics of root system architecture using nearisogenic lines of melon. Journal of the American Society for Horticultural Science, 133, 448-458.

    1. Gholizadeh-Roshanagh, S. (2016). Genetic diversity of three viruses of cucumber mosaic, watermelon mosaic and squash yellow mosaic in Varamin melon and cantaloupe fields. MSc. thesis, University of Tehran.
    2. Harel-Beja, R., Tzuri, …, & Katzir, N. (2010). A genetic map of melon highly enriched with fruit quality QTLs and EST markers, including sugar and carotenoid metabolism genes. Theoretical and Applied Genetics, 121, 511-533.

    1. Herman, R., Zvirin, Z., … & Goodman E. (2008). Characterization of Fusarium race 1.2 resistance in melon and mapping of a major QTL for this trait near a fruit netting locus 1. Proceeding of the IX eucarpia meeting on genetics and breeding of Cucurbitaceae, 149 – 156.
    2. Iathet, C., & Piluek, K. (2006). Heritability, heterosis and correlations of fruit characters and yield in Thai slicing melon (Cucumis melo var. Conomon makino). Kasetsart Journal, Natural Sciences, 40(1), 20-25.
    3. Jariani, P., Ramshini, H., Lotfi, M., Amini, F., Abtahi, H., & Ahmadvand, R. (2022). Development of cantaloupe (Cucumis melo) lines carrying Vat gene with favorable fruit traits. European Journal of Horticultural Science, 87(5), 1-9. DOI:17660/eJHS.2022/052
    4. Kalb, T. J., & Davis, D. W. (1984). Evaluation of combining ability, heterosis, and genetic variance for yield, maturity, and plant characteristics in bush muskmelon. Journal of the American Society for Horticultural Science, 109(3), 416-419.
    5. Kayak, N., & Türkmen, Ö. (2022). Reveling morphological variability in some S1 level melon genotypes. International Journal of Agricultural and Natural Sciences. 15(1), 109-124.
    6. Kerje, T., & Grum, M. (2000). The origin of melon, Cucumis melo: a review of the literature. Acta Horticulture 510, 37-44.
    7. Kitroongruang, N., Poo-Swang, W., & Tokumasu, S. (1992). Evaluation of combining ability, heterosis and genetic variance for plant growth and fruit quality characteristics in Thai-melon (Cucumis melo acidulus Naud.). Scientia Horticulturae, 50(1), 79-87.
    8. Knavel, D.E. (1990). Inheritance of a short-internode mutant of Mainstream muskmelon. Hortscience, 25, 1274-1275.
    9. Kurosky, K., Kuhn, K., Luton, J., & Rosenberg, S. (2016). Field guide to cucurbit diseases reference guide to cucumber, melon, pumpkin and watermelon diseases. Translated by Alizadeh, M. (1th ed). Miyad Andisheh Publication, 246 p.
    10. McCreight, J.D. (1983). A long internode mutant in muskmelon. Cucurbit Genetics Cooperative Rep. 6, 45.
    11. Mohammadi, R., Dehghani, H., & Karimzadeh, G. (2014a). Graphic analysis of trait relations of cantaloupe using the Biplot method. Journal of Plant Production, 21(4), 43-62. (In Persian with English abstract).
    12. Mohammadi, R., Dehghani, H., Karimzadeh, Q., Dane, F., & Akrami, M. (2014b). Study on relationships between yield and its components in Iranian cantaloupe genotypes. Iranian Journal of Horticultural Sciences, 45(1), 1-10. (In Persian with English abstract).
    13. Monforte, A.J., Eduardo, I., Abad S., & Arus, P. (2005). Inheritance mode of fruit traits in melon: Heterosis for fruit shape and its correlation with genetic distance. Euphytica, 144, 31-38.
    14. Monforte, A.J, Oliver, M., Gonzalo, M.J., Alvarez, J.M., Dolcet-Sanjuan, R., & Arus, P. (2004). Identification of quantitative trait loci involved in fruit quality traits in melon (Cucumis melo). Theoretical and Applied Genetics, 108, 750-758.
    15. Moreno, E., Obando, J.M., Dos-Santos, N., Fernandez-Trujillo, J.P.,Monforte, A.J., & Garcia-Mas, J. (2008). Candidate genes andQTLs for fruit ripening and softening in melon. Theoretical and Applied Genetics, 116, 589-602.
    16. Nanthakumar, S., Sankar, R.S., & Rameshkumar, D. (2021). Correlation and path analysis studies on yield and yield components in Muskmelon. International Journal of Plant and Soil Science, 33(21), 130-136.
    17. Naroui-Rad, M.R., & Rafezi, R. (2020). Integrated genetic components and machine learning approaches for better selection of traits in breeding of melon under high tunnel cultivation codition. Plant Cell Biotechnology and Molecular Biology, 21(37and 38), 36-46.
    18. Naroui-Rad, M.R., Koohkan, S., Fanaei, H.R., & Khajedad, M. (2014). Multivariate analysis to determine relationship between phenological traits with yield components in native melon population (Cucumis melo). Scientific Journal of Crop Science, 3(5), 48-55. (In Persian with English abstract).
    19. Obando, J., Fernandez-Trujillo, J.P., Martinez, J.A., Alarcon, A.L., Eduardo, I., Arus, P., & Monforte, A.J. (2008). Identification ofmelon fruit quality quantitative trait loci using near-isogenicJournal of the American Society for Horticultural Science, 133, 139-151.
    20. Obando-Ulloa, J.M., Eduardo, I., Monforte, A.J., & Fernandez-Trujillo,P. (2009). Identification of QTLs related to sugar and organic acid composition in melon using near-isogenic lines.Scientia Horticulturae, 121, 425-433.
    21. Ohara, T., Kojima, A., Wako, T., & Ishiuchi, D. (2001). Inheritance of suppressed-branching in melon and its association with some other morphological characters. Journal of the Japanese Society for Horticultural Science, 70, 341-345.
    22. Périn, C., Dogimont, C., Giovinazzo, N., Besombes, D., Guitton, L., Hagen, L., & Pitrat, M. (1999). Genetic control and linkages of some fruit characters in melon. Cucurbit Genetics Cooperative Rep, 22, 16-18.
    23. Pistorale, S.M., Abbott, L.A., & Adriana, A. (2008). Genetic diversity and broad sense heritability in tall wheatgrass (Thinopyrum ponticum). Ciencia e Investigación Agraria, 35, 213-218.
    24. Pitrat, M. (2008). Melon In: Prohens, J. & Nuez, F. (Eds.). Handbook of plant breeding-vegetables I. Springer, New York, P: 283–316.
    25. Pouyesh, A., Lotfi, M., Ramshini, H., Shamsitabar, A., & Armiyoun, E. (2017). Genetic analysis of yield and fruit traits in cantaloupe cultivars. Plant Breeding, 136, 569–577.
    26. Rajitha Nair, S., & Kumar, S. (2021). Innovation in hybrid seed production of vegetable Crops, A review. The Pharma Innovation Journal, 10(7), 1270-1275.
    27. Rakhi, R., & Rajamony, L. (2005). Variability, heritability and genetic advance in landraces of culinary melon (Cucumis melo ). Journal of Tropical Agriculture, 43(1-2), 79-82.
    28. Ramezani, F., Ramshini, H., Lotfi, M., Mortazavian, M.M., & Pourmombeini, S. (2020). SNP marker assisted selection for improving the sugar content in the cantaloupe. Iranian Journal of Horticultural Science, 51(1), 165-176.
    29. Rieseberg, L.H., Archer, M.A., & Wayne, R.K. (1999). Transgressive segregation, adaptation and speciation. Heredity, 83, 363-372.
    30. Robinson, D.C., Comstock, R.E., & Harvey, P.H. (1955). Genetic variances in open pollinated corn. Genetics, 40, 45-60.
    31. Roy, K.W. (1997). Fusarium solani on soybean roots: Nomenclature of the causal agent of sudden death syndrome and identity and relevance of solani form B. Plant Disease, 81, 259-266.
    32. Saadalla M.M., Shanahan J.F., & Quick J.S. (1990). Heat tolerance in winter wheat: I. hardening and genetic effects on membrane thermo stability. Crop Science, 30, 1243-1247.
    33. Shirali, A., Ramshini, H., & Sharezai, A. (2015). Isolation and identification of fungi associated with cantaloupe and root rot in the southeast of Tehran province.Sc. thesis. University of Tehran. 248 p.
    34. Tadmor, Y., Burger, J., & Katzir, N. (2010). Genetics of flavonoid, carotenoid, and chlorophyll pigments in melon fruit rinds. Journal of Agricultural and Food Chemistry, 58, 10722-10728.
    35. Upadhyaya, H.D., Sharma, S., & Gowda L.L. (2011). Major genes with additive effects for seed size in Kabuli chickpea (Cicer arietinum). Indian Academy of Sciences, 90(3), 1-4.
    36. Wall, J.R. (1967). Correlated inheritance of sex expression and fruit shape in Cucumis. Euphytica, 16, 199-208.
    37. Zalapa, J.E., Staub, J.E., & Mccreight, J.D. (2006). Generation means analysis of plant architectural traits and fruit yield in melon. Plant Breeding, 125(5), 482-487.
    38. Zalapa, J.E., Staub, J.E., & Mccreight J.D. (2008). Variance component analysis of plant architectural traits and fruit yield in melon. Euphytica, 162(1), 129-143.
    39. Zhang, Y., Kang, M.S., & Lamkey K.R. (2005). DIALLELSAS05: a comprehensive program for Griffings and Gardner-Eberhart analyses. Agronomy Journal, 97, 1097-1106.