Physico-Chemical Response and Seedling Growth of Three Aromatic Plants, Tanacetum parthenium, Achillea millefolium, and Zygophyllum eurypterum to Salinity Stress

Document Type : Research Article

Authors

• M. Ayaseh
• M. Kafi
• M. Khansefid
• M. Shokrpour
• R. Naderi

Department of Horticultural Science and Landscape Engineering, Faculty of Agriculture, University of Tehran, Kraj, Iran

https://doi.org/10.22067/jhs.2024.89514.1372

Abstract

Introduction
Today, a significant factor limiting plant growth is the rising salinity of soil and water, which poses challenges to food security, ecological stability, and agricultural systems. Numerous studies indicate that salinity hampers seed germination and early plant development by inducing osmotic stress, ion toxicity, and oxidative stress. Utilizing salinity-tolerant aromatic plants in saline areas can effectively enhance landscapes. Therefore, cultivating valuable plant species that meet high ecological demands may be the best strategy to mitigate salinity stress in agriculture and natural resources. These species can serve as alternative crops for oil seed production, food products, fodder, and both medicinal and ornamental uses. Tanacetum parthenium, Achillea millefolium, and Zygophyllum eurypterum are examples of medicinal and aromatic herbaceous perennials with low input requirements. They can be grown for various purposes, including ornamental use in dry and saline climates, as well as in the food, perfumery, cosmetics, and medicinal industries. Successful cultivation of these valuable plants in stressful ecosystems necessitates investigating their early growth responses to salinity stress. This research aims to identify suitable conditions for the successful establishment of these plants, ultimately leading to increased production.
 
Materials and Methods
In order to investigate the physicochemical responses and the early growth of seedlings in the shoot and root of three plants, T. parthenium, A. millefolium and Z. eurypterum to different levels of zero salinity stress (control), 1.5, 3 and 6 dS.m^-1, an experiment was conducted as factorial based on completely random design with three replications using a completely randomized design in a greenhouse under pot conditions in Department of Horticultural Science and Green Space Engineering at the University of Tehran. Seeds from three plant species—T. parthenium, A. millefolium, and Z. eurypterum—were obtained from the Research Institute of Forests and Rangelands (RIFR) in Karaj province, Iran. Prior to planting, the seeds were sterilized for one minute in a 2% sodium hypochlorite solution and then washed three times with sanitized water. The seeds were then planted in plastic pots (45, 66, and 23 cm) filled with a mixture of clay, sand, and perlite, at a depth of three centimeters, under greenhouse conditions. Sodium chloride was used to create salinity stress, exposing all studied plants to four salinity levels for a duration of six weeks. The greenhouse maintained a temperature of 20°C at night and 25°C during the day, with a relative humidity of 60-80%. All physiological and biochemical measurements were conducted after six weeks, once the plants were fully established. It is important to note that all samples were washed with distilled water post-sampling, the surface water was removed using filter paper, and samples were immediately stored at -80°C until measurements were taken.
Results and Discussion
The results of this study indicated that the concentration of photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll) decreased with increasing salinity levels, which correlated with a reduction in the growth length of both shoots and roots. The results indicated that under severe salinity stress, oxidative damage increased, leading to higher levels of fat peroxidation (MDA) and reduced cell membrane stability. This decrease in stability was linked to a reduction in photosynthetic pigments, ultimately disrupting optimal plant early growth. At all salinity levels, the total antioxidant capacity of A. millefolium leaves was greater than that of its roots. Among all three plants and salinity levels, the highest total antioxidant capacity of 78.57% was observed in the roots of Z. eurypterum under a salinity condition of 3 dS.m^-1. Conversely, the lowest total antioxidant capacity, at 25.38%, was found in the leaves of T. parthenium under non-saline conditions. The results indicated that the potassium to sodium ratio at equilibrium varied significantly depending on the plant species, plant organ, and salinity concentration and the highest ratio was found in the aerial parts of T. parthenium.
 
Conclusions
Overall, based on the biochemical and physiological responses to salinity stress, all three plants exhibited a relatively high tolerance. However, the T. parthenium plant demonstrated the greatest tolerance to salinity stress among them. Therefore, the cultivation of these three species, which possess ornamental, aromatic, and medicinal value, can serve as a viable solution for the ecological sustainability of saline ecosystems and their integration into urban landscaping.
 
Acknowledgement 
The authors are grateful for the scientific assistance of Dr. Behrouz Malekpour
 

Keywords

• Antioxidant capacity
• Aromatic plants
• Ion homeostasis
• Osmotic potential

Main Subjects

• Ornamental plants

©2024 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

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