علوم باغبانی
با همکاری انجمن علمی منظر ایران

تأثیر اسیدفولویک بر ویژگی‌های مورفو-فیزیولوژیکی و عمر گلجای رز رقم (Rosa hybrida c.v Dolce vita) تحت تنش شوری

نوع مقاله : مقالات پژوهشی

نویسندگان

مریم داننده 1
رسول آذرمی 1
موسی ترابی گیگلو 1
اسماعیل چمنی 1
زهرا اصلانی 2

1 گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

https://doi.org/10.22067/jhs.2025.92084.1410
چکیده
گل رز (Rosa hybrida) یکی از مهم‌ترین گل‌های شاخه ‌بریده در صنعت گلکاری است. شوری یکی از عوامل محیطی تأثیرگذار بر رشد و کیفیت این گیاه محسوب می‌شود. تغذیه مناسب می‌تواند نقش مهمی در افزایش تحمل گیاهان به تنش­های محیطی ایفا کند. این آزمایش در قالب طرح بلوک­های کامل تصادفی با چهار تکرار، در گلخانه تحقیقاتی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی اجرا گردید. تیمارهای آزمایشی شامل شاهد (بدون شوری و بدون اسیدفولویک)، شوری با غلظت 120 میلی­مولار، شوری + اسیدفولویک10 میلی­گرم در لیتر، شوری + اسیدفولویک30 میلی­گرم در لیتر، شوری + اسیدفولویک 60 میلی­گرم در لیتر، شوری + اسیدفولویک 90 میلی­گرم در لیتر، شوری + اسیدفولویک 120 میلی­گرم در لیتر بود. تنش شوری با استفاده از سدیم کلرید اعمال گردید و محلول غذایی روزانه متناسب با مرحله رشدی گیاه به‌میزان 100 تا 150 میلی‌لیتر به هر تیمار اضافه شد. نتایج نشان داد که اعمال تنش شوری با غلظت 120 میلی‌مولار باعث کاهش معنی­دار در وزن تر برگ، وزن تر ساقه، تعداد گل، عمر گلجای، قطر گل، محتوای نسبی آب برگ و میزان کلروفیل a، b و کل در مقایسه با شاهد شد. همچنین نتایج نشان داد که کاربرد اسیدفولویک تأثیر معنی­داری بر صفات مورد مطالعه داشت، به­طوری­که موجب افزایش شاخص‌های رشدی، فیزیولوژیکی و کیفی گل رز گردید. براساس یافته‌های این پژوهش، به‌کارگیری اسیدفولویک می­تواند در کاهش اثرات منفی تنش­های اسمزی و بهبود رشد و کارآیی گیاه رز در شرایط تنش شوری مؤثر باشد.

کلیدواژه‌ها

تنش غیرزیستی
گیاهان زینتی
مواد هیومیک
هیدروپونیک
موضوعات

عنوان مقاله English

Effect of Fulvic Acid on Morpho-Physiological Characteristics and the Vase Life of Rose Cut Flowers (Rosa hybrid c.v. Dolce vita) under Salinity Stress

نویسندگان English

M. Danandeh 1
R. Azarmi 1
M. Torabi Giglou 1
E. Chamani 1
Z. Aslani 2
1 Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده English

Introduction
Rosa hybrida is known as one of the most important cut flowers. Rosa hybrida is one of the most diverse and widespread plants in the Northern hemisphere in terms of growth habit, flower color and shape. Roses are mostly grown in the ornamental industries as cut flowers, potted plants and bedding plants due to their ornamental value. In addition, roses and their products have many applications in various industries such as beverages, cosmetics, food and pharmaceuticals. So, salinity stress is one of the environmental factors limiting the cultivation of this plant. Appropriate plant nutrition plays a significant role in raising the tolerance level of plants against various stresses.
 
Materials and Methods
This experiment was carried out in the form of a completely randomized design with four replications in the research greenhouse of the Faculty of Agriculture and Natural Resources of Mohaghegh Ardabili University. Experimental treatments included control, salinity with the concentration of 120 mM, salinity + fulvic acid 10 mg.L-1, salinity + fulvic acid 30 mg.L-1, salinity + fulvic acid 60 mg.L-1, salinity + fulvic acid 90 mg.L-1, salinity + fulvic acid 120 mg.L-1. After the plants were established in the original pots, they were placed on platforms one meter above the ground. On average, each pot was fed twice a day with a basal nutrient solution based on modified Hoagland nutrient solution. Experimental treatments were applied approximately 50 days after the plants were established. Salinity treatment was applied by sodium chloride. 100 to 150 ml of nutrient solution was added to each treatment according to the growth stage of the plant. The growth medium was rinsed once a week to minimize EC changes. The pH of the nutrient solution was adjusted to 6.5 with 37% hydrochloric acid. Flowers were harvested at the beginning of sepal opening (economic harvest stage) from a height of 45 cm of the flowering stem with a sharp knife. The stem was immediately placed in 500 ml bottles filled with 250 ml of distilled water containing 75 mg /L Sodium hypochlorite.
 
Results and Discussion
The results showed that the application of salt stress with a concentration of 120 mM caused a significant decrease in leaf fresh weight, stem fresh weight, number of flowers, vase life, flower diameter, relative water content of leaves and the amount of a, b and total chlorophyll in comparison with the control treatment. Also, the results showed that the application of fulvic acid had a significant effect on the studied traits. So that the application of fulvic acid increased the growth, physiological and quality parameters of roses. The results showed that leaf fresh weight, stem fresh weight, flower number, flower length and vase life were significantly affected by salinity and fulvic acid treatments. So that under salinity stress conditions, the highest leaf fresh weight (3.56 g) and stem fresh weight (27.51 g) were obtained in the 30 mg.L-1 fulvic acid treatment. Also, the highest number of flowers (2.25), flower length (45.51 mm) and vase life (10.50) were observed in the 30 mg.L-1 fulvic acid treatments in the non-salinity conditions, and the lowest levels of these traits were obtained in the salinity and non-fulvic acid treatments. Fulvic acid increases the availability of the elements in the plant by chelating the minerals including, potassium, magnesium, zinc, calcium, iron, copper and increasing enhance the growth of lateral roots.
 
Conclusion
It seems that the application of fulvic acid can be useful in removing osmotic stress and improving the growth and efficiency of rose plants under salt stress conditions. This experiment shows that adding fulvic acid to saline water can reduce the effects of salinity stress on rose plants. So that using different concentrations of fulvic acid (10, 30, 60 and 90 mg.L-1), especially the concentration of 30 mg.L-1, improved the absorption of nutrients such as nitrogen, potassium and calcium and photosynthetic pigments and moderated the adverse effects of salinity stress.

کلیدواژه‌ها English

Abiotic stress
Humic substances
Hydroponics
Ornamental plants

Authors retain the copyright. This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).
  1. Abaszadeh Faruji, R. M., Shoor, A., Tehrani Far, B., & Safari. N. (2018). Effects of humic acid and fulvic acid on some morphological characteristics of geranium. Journal of Horticultural Science, 32(1), 35-50.(in Persian with English abstract). https://doi.org/10.22067/jhorts4.v31i3.57849
  2. Abdolmaleki, M., Khosh, K. M., Eshghi, S., & Ramezanian, A. (2015). Improvement in vase life of cut rose cv.“Dolce Vita” by preharvest foliar application of calcium chloride and salicylic acid. International Journal of Horticultural Science and Technology‏, 2(1), 55-66. https://doi:22059/ijhst.2015.54264
  3. Abul, B., Giasuddin, A. M., Kanel, S., & hoi, H.C. (2007). Adsorption of humic acid onto nanoscale zerovalent iron and its effect on arsenic removal. Environmental Science and Technology, 41(6), 2022-2027.https://doi: 10.1021/es0616534.
  4. Ali, E. F., Al-Yasi, H. M., Issa, A. A., Hessini, K., & Hassan, F. A. (2022). Ginger extract and fulvic acid foliar applications as novel practical approaches to improve the growth and productivity of Damask Rose. Plants, 11(3), 412. https://doi:3390/plants11030412
  5. Arnon, A. N. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112-121.
  6. Aminifard, M. H., Aroiee, H., Nemati, H., Azizi, M., & Hawa, Z. E. (2012). Fulvic acid affects pepper antioxidant activity and fruit quality. African Journal of Biotechnology, 11, 13179-13185. https://doi:10.5897/AJB12.1507
  7. Balakumbahan, R., & Rajamani, K. (2010). Effect of biostimulants on growth and yield of senna (Cassia angustifoliavar1). Journal of Horticultural Science and Ornamental Plants, IDOSI Publication, 2, 16-8.
  8. Bayat, H., Shafie, F., Aminifard, M. H., & Daghighi, S. (2021). Comparative effects of humic and fulvic acids as biostimulants on growth, antioxidant activity and nutrient content of yarrow (Achillea millefolium). Scientia Horticulturae, 279, 109912. . https://doi:10.1016/j.scienta.2021.109912
  9. Canellas, L. P, Teixeira Junior, L. R. L, Dobbss, L. B, Silva, C. A, Medici, L. O., Zandonadi, D. B., & Façanha, A. R. (2008). Humic acids cross interactions with root and organic acids. Annals of Applied Biology, 153,157-66. https://doi:1111/j.1744-7348.2008.00249.x
  10. Calvo, P., Nelson, L., & Kloepper, J. W. (2014). Agricultural uses of plant biostimulants. Plant and Soil, 383, 3-41. https://doi:1007/s11104-014-2131-8
  11. Cai, X., Niu, G., Starman, T., & Hall, C. (2014). Response of six garden roses (Rosa × hybrida) to salt stress. Scientia Horticulturae, 168, 27-32. https://doi:10.1016/j.scienta.2013.12.032
  12. Capstaff, N. M., Morrison, F., Cheema, J., Brett, P., Hill, L., Muñoz-García, J. C., Khimyak, Y. Z. M, Domoney, C., & Miller, A. J. (2020). Fulvic acid increases forage legume growth inducing preferential up-regulation of nodulation and signalling-related genes. Journal of Experimental Botany, 71, 5689-5704. https://doi:10.1093/jxb/eraa283.
  13. Cotteni, A. (1980). Methods of plant analysis. In: Westerman R.L. (Ed.), Soil and Plant Testing. FAO Soil Bulletin, pp. 64-100.
  14. Dinler, B. S., Gunduzer, E., & Tekinay, T. (2016). Pre-treatment of fulvic acid plays a stimulant role in protection of soybean (Glycine max) leaves against heat and salt stress. Acta Biologica Cracoviensia Botny, 58, 29-41.
  15. https://doi: 10.1515/abcsb-2016-0002.
  16. Frairevelazquez, S., & Balderas-Hernández, V. E. (2013). Abiotic stress in plants and metabolic responses. In: Vahdati K, Leslie C (Eds.), Abiotic Stress-Plant Responses and Applications in Agriculture. Intechopen, pp. 25-48. https://doi:10.5772/54859
  17. Gaurav, A. K., Namita, D., Raju, M., Ramkumar, M., Singh, B., Singh, S. G., Krishnan S., Panwar, M., & Sevanthi, A. (2021). Genetic diversity analysis of wild and cultivated Rosa species of India using microsatellite markers and their comparison with morphology based diversity. Journal of Plant Biochemistry and Biotechnology, 31(1) 1-10. https://doi:1007/s13562-021-00655-3
  18. Gabr, S., Abouelsaad, I., Brengi, S., & Gouda. A. (2022). Salt stress mitigation of spinach plants as affected by silicon and fulvic acid. Advanced Agricultural Research and Technology Journal, 27, 26-42. https://doi:3390/plants12122359
  19. Gomaa, M. A., Kandil, E. E., & Gharib, A. (2019). Response of wheat plants to seaweed extracts and fluvic acid under irrigation with drainage water. Egyptian Academic Journal of Biological Sciences, 10, 35-44. https://doi:21608/eajbsh.2019.44518
  20. Hatami, E., Shokouhian, A. A., Ghanbari, A. R., & Naseri, L. A. (2018). Alleviating salt stress in almond rootstocks using of humic acid. Scientia Horticulturae, 237, 296-302. https://doi:1016/j.scienta.2018.03.034
  21. Hajlaoui, H. N., Ayeb, E. l., Garrec, J. P., & Denden, M. (2010). Differential effects of salt stress on osmotic adjustment and solutes allocation on the basis of root and leaf tissue senescence of two silage maize (Zea mays) varieties. Industrial Crops and Products, 31, 122-130. . https://doi:10.1016/j.indcrop.2009.09.007
  22. Hassanvanda, H., Rezaei Nejada, A., & Fanourakisb, D. (2019). Morphological and physiological components mediating the silicon-induced enhancement of geranium essential oil yield under saline conditions. Industrial Crops and Products, 134, 19-25. https://doi: 1016/j.indcrop.2019.03.049
  23. Hassan, F. A. S., Mazrou, R., Gaber, A., & Hassan, M. (2020). Moringa extract preserved the vase life of cut roses through maintaining water relations and enhancing antioxidant machinery. Postharvest Biology and Technology, 164, 111156. https://doi.org/10.1016/j.postharvbio.2020.111156
  24. Hosseini Farahi, M. B., Kholdebarin, S., Eshghi, B., Jamali, H., & Roosta, R. (2019). Changes in plant growth substances, contents and flower quality of rose cv.‘Dolce Vita’in response to nitrogen sources under soilless culture Journal of Plant Nutrition, 42(9), 1047-1060. https://doi.org 10.1080/01904167.2019.1578373
  25. Heidari Sharif Abad, H. (2011). Plants and Salinity. Research Institute of Forests and Rangelands Publications, Tehran, Iran. 199 p. (In Persian).
  26. Jesmin, A., Anh, L. H., Mai, N. P., Khanh, T. D., & Xuan, T. D. (2023). Fulvic acid improves salinity tolerance of rice seedlings: evidence from phenotypic performance, relevant phenolic acids, and momilactones. Plants, 12(12), 2359. https://doi.org 3390/plants12122359
  27. Khaled, H., & Fawy, H. (2011). Effect of different levels of humic acids on the nutrient content, plant growth and soil properties under conditions of salinity. Soil Water Research, 6, 21-29. https://doi.17221/4/2010-SWR
  28. Li, M., Su, Y., Chen, Y., Wan, R., Zheng, X., & Liu, K. (2016). The effects of fulvic acid on microbial denitrification: Promotion of NADH generation, electron transfer, and consumption. Applied Microbiology and Biotechnology, 100, 5607-18. https://doi. 10.1007/s00253-016-7383-1.
  29. Maraei, R., Eliwa, N., & Aly, A. (2019). Use of some biostimulants to improve the growth and chemical constituents of sweet pepper. Potravinarstvo Slovak Journal of Food Sciences,13, 553-561. https://orcid.org/0000-0003-3295-8806
  30. Munns, R. (2002). Comparative physiology of salt and water stress. Plant Cell Environment, 25, 239-250. https://doi.org/1046/j.0016-8025.2001.00808.x
  31. Munns, R., Richard, A., James, B., & Andre, L. (2006). Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany, 57(5), 1025-1043.
  32. Nasir, M., Sattar Khan, A., Basra, S. M. A., & Malik, A. U. (2016). Foliar applications of moringa leaf extract, potassium and zinc influence yield and fruit quality of ‘Kinnow’ mandarin. Horticultural Science, 210, 227-235 : https://doi.org/1016/j.scienta.2016.07.032
  33. Natesan, R., Kandasamy, S., Thiyageshwari, S., & Boopathy, P. M. (2007). Influence of lignite humic acid on the micronutrient availability and yield of blackgram in an alfisol. Iranian Journal of Science, 7, 1198-1206. https://doi.org/1093/jxb/erj100
  34. Nikbakbakht, A., Kafi, M., Babalar, M., Xia, Y. P., Luo, A., & Etemadi, N. A. (2008). Effect of humic acid on plantgrowh, nutrient uptake and post havest life of Gerbera. Journal of Plant Nutrition, 31, 2155-2167 https://doi.org/1080/01904160802462819
  35. Nikoogoftar-Sedghi, M., Rabiei, V., Razavi, F., Molaei, S., & Khadivi, A. (2024). Fulvic acid foliar application: a novel approach enhancing antioxidant capacity and nutritional quality of pistachio (Pistacia vera). BMC Plant Biology, 24(1), 241. https://doi.org/10.1186/s12870-024-04974-0
  36. Olaetxea, M., De Hita, D., Garcia, C. A., Fuentes, M., Baigorri, R., Mora, V., Garnica, M., Urrutia, O., Erro, J., & Zamarreño, A. M. (2018). Hypothetical framework integrating the main mechanisms involved in the promoting action of rhizospheric humic substances on plant root- and shoot- growth. Applied Soil Ecology, 123, 521-537.
  37. Parida, A. K., & Das, A. B. (2005). Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety, 60, 324-349. https://doi.org/10.1016/j.ecoenv.2004.06.01
  38. Qadir, M., Quillérou, E., Nangia, V., Murtaza, G., Singh, M., Thomas, R. J., Drechsel, P., & Noble, A. D. (2014). Economics of salt‐induced land degradation and restoration. In Natural Resources Forum, 4, 282-295. https://doi.org/1111/1477-8947.12054
  39. Roohani, N. S., Nemati, S. H, Moghadam, M., & Ardakanian, V. (2014). The role of humic acid on morphological, physiological and biochemical characteristics of three varieties of radish (Raphanus sativus ) under salt stress. M.Sc. Thesis, Ferdowsi University of Mashhad, Mashhad, Iran.
  40. Sun,, Qiu, C., Ding, Y., Wang, Y., Sun, L., Sun, L., Fan, K., Gai, Z., Dong, G., & Wang, J. (2020). Fulvic acid ameliorates drought stress-induced damage in tea plants by regulating the ascorbate metabolism and flavonoids biosynthesis. BioMed Central Genom, 21, 1-13. https://doi: 10.1186/s12864-020-06815-4.
  41. Sun, H. Y., Yoo, K. S., & Suh, S. G. (2014). Effect of foliar application of fulvic acid on plant growth and fruit quality of tomato (Lycopersicon esculentum). Horticulture, Environment, and Biotechnology, 55, 455-461.
  42. Serrano, R., & Rodriguez-Navarr, A. (2001). Ion homeostasis during salt stress in plants. Current Opinion in Cell Biology, 13, 399-404.
  43. Siddiqi, E. H., Ashraf, M., Hussain, M., & Jamil, A. (2009). Assessment of intercultivar variation for salt tolerance in safflower (Carthamus tinctorius) using gas exchange characteristics as selection criteria. Pakistan Journal of Botany, 41(5), 2251-2259.
  44. Tavakkoli, E., Fatehi, F., Coventry, S., Rengasamy, P., & McDonald, G. K. (2011). Additive effects of Na+ and Cl− ions on barley growth under salinity stress. Journal of Experimental Botany, 62, 2189-2203.
  45. Tabatabaei, S. J. (2006). Effect of salinity and N on the growth photosynthesis and N status of oliv (Olea europaea) trees. Scientia Horticulturae, 108(4), 432-438. https://doi.org/10.1016/j.scienta.2006.02.016
  46. Turner, C. (1981). Techniues and experimental approaches for measurement of plant water status. International Journal of Plant-Soil Relationships, 58, 339-366. https://doi.org/ 10.1007/BF02180062
  47. Yu, Y., Xu, T., Li, X., Tang, J., Ma, D., Li, Z., & Sun, J. (2016). NaCl-induced changes of ion homeostasis and nitrogen metabolism in two sweet potato (Ipomoea batatas ) cultivars exhibit different salt tolerance at adventitious root stage. Environmental and Experimental Botany, 129, 23-36. https://doi.org/ 10.1016/j.envexpbot.2015.12.006
  48. Yazdani, B. (2010). Effect of different concentrations of humic acid and fulvic acid on the qualitative and quantitative characteristics of Gerbera jamesonii. M.Sc. Thesis, Isfahan University of Agricultural Sciences, Isfahan, Iran. (In Persian)
  49. Veatch-blohm, M. E., Malinowski, M., & Keefer, D. (2012). Leaf water status, osmotic adjustment and carbon assimilation in colored calla lilies in response to saline irrigation. Scientia Horticulturae, 144, 65-73. https://doi.org/1016/j.scienta.2012.06.036
  50. Varanini, Z., & Pinton, R. (2000). Direct versus indirect effects of soil humic substances on plant growth and nutrition. In The Rhizosphere, 1st Ed.. Willig, S., Varanini, Z., Nannipieri, P., (Eds.). CRC Press: Boca Raton, FL, USA, pp. 157–174.
  51. Zlesak, D. C. (2007). Rose: Rosa x hybrida. In Flower Breeding and Genetics: Issues, Challenges and Opportunities for the 21st Century: 695-740: Number of 695-740 pp.

 

ارسال نظر در مورد این مقاله
نام را وارد کنید.
نشانی پست الکترونیکی را به درستی وارد کنید.
وابستگی سازمانی را به درستی وارد کنید.
توضیحات را وارد کنید (حداقل 50 حرف)
CAPTCHA Image
شناسه امنیتی را به درستی وارد کنید.

  • تاریخ دریافت 19 بهمن 1403
  • تاریخ بازنگری 23 اردیبهشت 1404
  • تاریخ پذیرش 24 اردیبهشت 1404
  • تاریخ اولین انتشار 24 اردیبهشت 1404

صفحه اصلی

اعضای هیات تحریریه

اصول اخلاقی انتشار مقاله

تماس با ما