نوع مقاله : مقالات پژوهشی
نویسندگان
1 استادیار گروه تولیدات گیاهی، دانشگاه تربتحیدریه
2 دانشگاه فردوسی مشهد
3 دانش‌آموخته کارشناسی رشته گیاهان دارویی و معطر، گروه تولیدات گیاهی، دانشگاه تربتحیدریه
چکیده
اسید هیومیک به عنوان یک اسید آلی، بدون اثرات مخرب زیستمحیطی میتوانـد در جهـت تغذیه صحیح سبزیها و بهبود کمی و کیفی آن استفاده شود.در همین راستا آزمایش حاضر به منظور بررسی کاربرد غلظتهای مختلف اسید هیومیک در زمانهای مختلف و اثرات آن بر شاخص‌های کمی و کیفی اسفناج به صورت فاکتوریل در قالب طرح کاملاً تصـادفی با سه تکرار در گلخانه تحقیقاتی دانشگاه تربتحیدریه انجام شد. تیمارها شامل سه غلظت صفر، 3 و 6 میلیلیتر در لیتر اسید هیومیک و دو زمان کاربرد یک هفته و دو هفته کاربرد آن بود. با کاربرد 3 میلیلیتر در لیتر اسید هیومیک صفات مورفولوژیک گیاه اسفناج شامل تعداد برگ، طول برگ، عرض برگ و ارتفاع بوته به ترتیب 21، 59، 134 و 75 درصد افزایش نسبت به شاهد (غلظت صفر) نشان دادند. همچنین در این غلظت صفات فیزیولوژیک شامل شاخص کلروفیل، کلروفیل a، کلروفیل b، سطح برگ، تاجپوشش، کاروتنوئید و زیستتوده به ترتیب 48، 59، 56، 45، 108، 31 و 275 درصد افزایش نسبت به شاهد (غلظت صفر) داشتند. بر اساس نتایج بین غلظت 3 و 6 میلیلیتر اسید هیومیک تفاوت معنیداری وجود نداشت. در بیشتر صفات زمان کاربرد دو هفته نسبت به یک هفته از مقادیر بالاتری برخوردار بود بطوریکه مقدار زیستتوده اسفناج در زمان کاربرد دو هفته 156 و در زمان کاربرد یک هفته 74 میلیگرم بود. بر اساس نتایج آزمایش حاضر کاربرد اسید هیومیک در غلظت 3 میلیلیتر در لیتر میتواند باعث بهبود صفات مورفوفیزیولوژیک و تولید گیاه اسفناج شود.
کلیدواژه‌ها
عنوان مقاله [English]
The Effect of Concentration and Time of Application of Humic Acid on Morpho-physiological Characteristics of Spinacia oleracea
نویسندگان [English]
- Mahboobeh Naseri 1
- Hossein Arouiee 2
- Maryam Mohammadi 3
1 Assistant Professor Department of Plant Production, University of Torbat Heydariyeh
2
3 Graduate Master, Department of Plant Production, University of Torbat Heydariyeh
چکیده [English]
Introduction: Spinacia olerace is the most important leaf vegetable of the Chenopodiacea family, which has a special place in human nutrition because of its variety of minerals and vitamins. Cultivation of this plant in Iran is several thousand years old and accordingly Iran is considered as the main place and a source of spinach in the world. Growth of plants is affected by genetics, environmental conditions, growing season, nutrients, and soil, harvesting method, temperature, intensity and quality of light. Among these factors, soil nutrients have a significant influence on plant growth and yield. Nowadays, the consumption of organic matter as fertilizer due to its high cost and limited availability is not sufficient and the major nutritional needs of plants are met by chemical fertilizers, which can lead to environmental problems and, over time, reduced yields. In this connection humic acid is a naturally occurring organic compound and contains 2% to 5% organic matter. Humic acid can be used to seedling nutrition and improve its quality and quantity. Given the recent use of organic acids such as humic acid to improve crops and horticulture, but little research has been done on transplant birth, this experiment was conducted to investigate the application of different levels of humic acid in irrigation water at the time of irrigation. Different effects and their effects on growth indices and spinach production were investigated.
Material and Methods: The present experiment was conducted to investigate the application of different concentrations of humic acid at different times and its effects on quantitative and qualitative indices of spinach in a factorial completely randomized design with three replications in research greenhouse at Torbat Heydariyeh University. Treatments consisted of three concentrations of humic acid (0, 3 and 6 ml/L) at two application times (one-week and two-week). The mean daily greenhouse temperature at the time of plant growth was 25°C and the mean nighttime temperature was 18°C, the average relative humidity was 60%. After seedlings were planted in the main pots at four-leaf stage, they were irrigated with humic acid (0, 3 and 6 ml/L) at different intervals once a week, twice weekly. The humic fertilizer used belonged to Green Seed Company, containing 24% humic acid and 3% folic acid and 2% potassium. Five weeks after treatment, the traits were measured. Transplant growth indices including number of leaves (by counting the number of shoots per plant), leaf length and width, leaf area, canopy, height, chlorophyll index, chlorophyll a, b, carotenoid and dry weight of each plant were determined.
Results and Discussion: Based on the results of this experiment, the effect of humic acid concentration on morphological and physiological traits of spinach transplant was significant. The highest leaf number (4.6) at 3 ml concentration and the lowest number (3.5) were at zero concentration. These traits were subjected to humic acid titer, but the increase in concentration had no significant effect on these traits. The increased number of leaves and traits mentioned in the early stages of transplant growth is probably due to the rapid expansion of the root system of the plant at high concentrations of humic acid, which in turn leads to increased nutrient uptake, better plant growth and subsequent growth. Leaf number and other traits become leaf dependent. The results showed that the highest chlorophyll a (1.8 ml/g fresh leaf), chlorophyll b (2.5 ml/ml fresh leaf), carotenoids (7.1 ml/ml). Fresh leaf g) and biomass (150 mg) at 3 ml concentration and the lowest at zero concentration. The highest chlorophyll index (74.1 ml/ml fresh leaf g) was also found in the concentration of 6 ml and the lowest was obtained from zero concentration. Humic acid increased spinach transplant biomass by increasing the amount of photosynthetic pigments and leaf area or the same photosynthetic capacity. In most of the traits, the two-week application time was higher than the one-week, so that spinach transplant biomass increased by 110% over the two-week application period. So that spinach transplant biomass was 156 at the two-week application and 74 mg at the one-week application. Based on the results of this experiment, humic acid application can improve the quantitative and qualitative traits of spinach transplant and its production.
Conclusion: Production of vegetable seedlings have an important role in the production and olericulture economy. Specializing in different activities in the vegetable production process will simplify the production process and increase efficiency. This means that the best conditions for seedling growth should be provided during transplanting. Application of humic acid organic fertilizer instead of chemical fertilizer can reduce environmental pollution in line with sustainable agriculture and healthy eating with vegetables to prevent the accumulation of chemicals in the human body to be effective. In this regard, the results of this study showed that using low humic acid as organic fertilizer can improve morphophysiological traits of spinach transplant.
کلیدواژه‌ها [English]
- Carotenoid
- Chlorophyll a
- Chlorophyll b Humus
- Organic matter
-
1- Aiken G.R., McKnight D.M., Wershaw R.L., and Mac Carthy P. 1985. Humic Substances in Soil, Sediment, and Water. New York. USA: Wiley InterScience.
2- Aisha H.A., Shafeek M.R., Asmaa MR., and M. El-Desuki. 2014. Effect of Various Levels of Organic Fertilizer and Humic Acid on the Growth and Roots Quality of Turnip Plants (Brassica rapa). Current Science International 3(1): 7-14.
3- Arnon A.N. 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal 23: 112-121.
4- Askari M., Habibi D., and Naderi G. 2012. Effect of vermicompost, plant growth, promoting rhizobacteria and humic acid on growth factor of Mentha piperata L. in central Province. Journal of Agriculture and Plant Breeding 4: 41-54.
5- Atiyeh R.M., Lee S., and Edwards C.A. 2002. The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology 84: 7-14.
6- Ayas H., and Gulser F. 2005. The effect of sulfur and humic acid on yield components and macronutrient contents of spinach. Journal of Biological Sciences 5(6): 801-804.
7- Chamani E., Bonyadi M., and Ghanbari A. 2016. Effects of Salicylic acid and Humic acid on Vegetative Indices of Periwinkle (Catharanthus roseus L.). Journal of Horticultural Science29(4): 631-641.
8- Chen Y., and Aviad T. 1990. Effects of humic substances on plant growth. PP. 161-186. Humic Substances in Soil and Crop Science: Selected Readings, SSSA and ASA, Madison, WI, USA.
9- Dalvand M., Solgi M., and Khaleghi A. 2018. Effects of foliar application of humic acid and drought stress on growth and physiological characteristics of marigold (Taget erecta). Journal of Science and Technology of Greenhouse Culture 9(2): 67-80
10- Fatemi H., Ameri A., Aminifard M.H., and Arvii H. 2011. The Impact of Humic Acid on Essence and Growth Characteristics in Basil, First National Conference on New Issues in Agriculture, Saveh, Islamic Azad University, Saveh Branch.
11- Fallah M., Peyvast G., Olfati J., and Sammak B. 2014. Effects of chemical fertilization and organic fertilizer on spinach (Spinacia oleracea L.) yield and nitrate accumulation. Journal of Plant Production Research 21(1): 49-68.
12- Heidari M., and Khahlil S. 2014. Effect of humic acid and phosphorus fertilizer on seed and flower yield, photosynthetic pigments and mineral elements concentration in sour tea (Hisbiscus sabdariffa L.). Iranian Journal of Field Crop Science 45(2): 191-199.
13- Kafi M., Daneshvar Hakimi Meybodi N., Nikbakht A., Rejali F., and Deneshkhah M. 2013. Effect of humic acid and mycorrhiza fungi on some characteristics of “Speedy green” perennial ryegrass (Lolium perenne L.). Journal of Science and Technology of Greenhouse Culture 4(1): 49-59.
14- Kauser A., and Azam F. 1985. Effect of humic acid on wheat seeding growth. Journal Environmental and Experimental Botany 25: 245-252.
15- Maccarthy P. 2001. The principles of humic substances. Soil Science 166: 738–751.
16- Mackowiak C.L., Grossl P.R., and Bugbee B.G. 2001. Beneficial effects of humic acid on micronutrient availability to wheat. Soil Science 65: 1744-1750.
17- Mobasser H.R., Pourmand H., Akbari Nodehi D., and Ghasemi M. 2016. Bio-refining effect of silicon on cadmium and lead in plant tissues of Spinach (Spinacia oleracea L.). Journal of Soil Management and Sustainable Production 5(4): 175-184.
18- Nardi S., Pizzeghello D., Muscolo A., and Vianello A. 2002. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry. 34: 1527-1536.
19- Nasiri D.A., Makarian H., Varnaseri G.V., and Salarian N. 2018. Investigation of effect of humic acid and vermicompost application on yield and yield components of cumin (Cuminum cyminum L.). Applied Research in Field Crops 31(1): 93-113.
20- Norman Q., Stephen L., Clive A., and Rola A. 2003. Effects of humic acids derived from cattle, food and paper-waste vermicomposts on growth of greenhouse plants. International Journal of Current Research in Biosciences and Plant Biology 47: 741-744.
21- Rostami G., Moghaddam M., Saeedi Pooya E., and Ajdanian L. 2019. The effect of humic acid foliar application on some morphophysiological and biochemical characteristics of spearmint (Mentha spicata L.) in drought stress conditions. Journal of Environmental Stresses in Crop Sciences 12(1): 95-110.
22- Sadeghi Pour M. 2010. Nitrogen Use Efficiency of Spinach. Journal of Water and Soil 24(2): 244-253.
23- Sánchez-Sánchez A., Sánchez-Andreu J., Juárez M., Jordá J., and Bermúdez D. 2002. Humic substances and amino acids improve effectiveness of chelate FeEDDHA in lemon trees. Journal of Plant Nutrition25: 2433-2442.
24- Shahsavan Markadeh M., and Chamani E. 2014. Effects of various concentrations and time of humic acid application on quantitative and qualitative characteristics of cut stock flower (Matthiola incana ‘Hanza’). Journal of Science and Technology of Greenhouse Culture 5(3): 157-171.
25- Sharif M., Khattak R.A., and Sarir M.S. 2002. Effect of different levels of lignitic coal drived humic acid on growth of maize plants. Communication in Soil Science and Plant Analysis 33: 3567-3580. (In Persian)
26- Souri M.K.F. 2018. Physiological characteristics of tomato seedlings under application of chemical and organic-based fertilizers. Journal of Soil and Plant Interactions 8(4): 67-77.
27- Talebi P., Jabbarzadeh Z., and Rasouli Sadaghiani M. 2017. Effect of application mode and different concentrations of humic acid on yield and content of nutrients uptake of Rosa chinensis var. Minima ‘Baby masquerade‘.Journal of Crops Improvement 18(4): 789-804.
28- Türkmen Ö., Dursun A., Turan M., and Erdinç Ç. 2004. Calcium and humic acid affect seed germination, growth, and nutrient content of tomato (Lycopersicon esculentum L.) seedlings under saline soil conditions. Acta Agriculturae Scandinavica 7: 168-174.
)
ارسال نظر در مورد این مقاله