Document Type : Research Article

Authors

Sari Agricultural Sciences and Natural Resources University

Abstract

Introduction: Rosemary (Rosmarinus officinalis L.) is a perennial, ever green and fragrant plant belongs to Lamiaceae family. Vegetative parts of this plant have essential oil and compounds with anti oxidant and antibacterial properties which are used extensively in pharmaceutical, food and cosmetic industries. The use of biofertilizers such as vermicompost and Nitroxin could have beneficial effect on production of rosemary by increasing the production of plant growth hormones and the availability of macro and micro nutrients in growing media.
Materials and Methods: The effect of vermicompost and Nitroxin biofertilizers was investigated on growth, yield, the amounts of photosynthetic pigments, flavonoid, essential oil percentage and yield of rosemary. The experiment was based on a randomized complete block design with two factors, including vermicompost (0, 10, 20, 30 and 40% w/w) and Nitroxin (inoculated and non-inoculated with Nitroxin) with four replications. This research was done at Sari Agricultural Sciences and Natural Resources University, Sari, Iran, in 2012-2013. Uniform one-year old rooted rosemary cuttings were selected for this experiment. Before planting, rooted cuttings were treated in diluted Nitroxin solution in water (1:10) for 10 minutes. After planting, rosemary plants were fertilized twice by Nitroxin for every 45 days according to the producing company recommendation. During growth period, irrigation was done according to plants requirement. At the end of experiment, parameters such as plant height, shoot fresh and dry weight, root dry weight, chlorophyll a, total chlorophyll, leaf flavonoid and essential oil yield were measured. Data was analyzed using standard analysis of variance (ANOVA) using the general linear models procedure of SAS, (version 9.1; SAS Institute, Cary, N.C.). Differences among means were tested by least significant difference (LSD) (p ≤ 0.05).
Results and Discussion: Obtained results showed that the application of different levels of vermicompost and Nitroxin inoculation and their interactions had significant effect on some rosemary morphological and physiological properties such as plant height, shoot fresh and dry weight, root dry weight, chlorophyll a, total chlorophyll, leaf flavonoid and essential oil yield. The interaction of vermicompost and Nitroxin were highly significant for shoot weight. The highest fresh weight (71.55 g) for each plant were obtained from 40% vermicompost with Nitroxin inoculated treatment. The lowest fresh weight (45.62 g and 44.4g) belonged to 10% vermicompost and control treatments, respectively. Shoot dry weight yield was also affected by vermicompost with Nitroxin interaction and was highly significant. The highest dry weight (31.37 g) was obtained from 40% vermicompost with Nitroxin inoculated treatment while the lowest dry weight (18.68 g and 18.79 g) was observed in control and Nitroxin treatment, respectively. The amount of chlorophyll a and total chlorophyll were affected by vermicompost with Nitroxin interaction too. The highest amount of chlorophyll a (25.89 mg/g) was observed in 30% vermicompost treatment. The highest amount of chlorophyll b (2.13 and 1.81 mg/g) belonged to 20% and 30% vermicompost treatments, respectively and , the highest amount of total chlorophyll (27.7 mg/g) was measured in 20% vermicompost in combination with Nitroxin inoculated treatment. The highest amount of leaf flavonoid (6.25 mg/g) was determined in 10% vermicompost with Nitroxin inoculated treatment. The highest essential oil yield (0.263 and 0.272 g per plant) was obtained from 20% and 30% vermicomposts treatments, respectively. According to the presented results, amendment of growing media with vermicompost and Nitroxin inoculation significantly increased quantity and quality properties of rosemary.
Conclusions: The presented results showed that the use of vermicompost, and Nitroxin separately or in combination have positive effect on growth yield, pigments formation and essential oil yield of rosemary. The increased fresh and dry yield of rosemary in this study could be the result of optimum condition of growth media provided by vermicompost. The use of vermicompost increases water holding capacity and better ventilation environment for plant roots. In addition, the presence of vermicompost in growing media provides greater nutrient availability for plants and therefore increases plant fresh and dry yield. This greater nutrient availability could be due to increased microbial population and their activities in vermicompost- amended growth media.

Keywords

1- Adediran J. A., Taiwo L. B., Akande M. O., Sobulo R. A. and Idowu O. J. 2004. Application of organic and inorganic fertilizer for sustainable maize and cowpea yields in Nigeria. Journal of Plant Nutrition, 27: 1163-1181.
2- Anwar M., Patra D. D., Chand S., Alpesh K., Naqvi A. A., and Khanuja S. P. S. 2005. Effects of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation and oil quality of French basil. Communications in Soil Sciences and Plant Analysis. 36: 1737-1746.
3- Arancon N., Edwards C.A., Bierman P., Welch C and Metzger J.D., 2004. Influences of vermicomposts on field strawberries: 1. Effects on growth and yields. Bioresource Technology. 93: 145-153.
4- Carruba A., La Torre R., and Matranga A. 2002. Cultivation trials of some aromatic and medicinal plants in a semiarid Mediterranean environment. Proceeding of an international conference on MAP. Acta Horticulture (ISHS). 576: 207-213.
5- Chang CC., Yang MH., Wen HM and Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Food Drug Anal. 10: 178 – 82.
6- Croteau r., Burbott A.Y. and Lommis W.D. 1972. Biosynthesis of monoed sesquiterpenes in peppermint. phytochemistry. 11:2937-2948.
7- Darzi M.T., Ghalavand A., and Rejali F. 2008. Effect of mycorrhiza, vermicompost and phosphate biofertilizer application on flowering, biological yield and root colonization in fennel (Foeniculum vulgare Mill.). Iranian Journal of Crop Sciences. 10 (1):88-109.
8- Dehghani moshkani M. R., Naghdi badi H., Darzi M.T., Mehrafarin A., Rezazadeh Sh., and Kadkhoda Z. 2011. Effect of biological and chemical fertilizers on yield and quality of Shirazi chamomile (Matricaria recutita L.). Journal of Medicinal Plants. 10(2): 35-48.
9- Ebrahimzadeh J. 1998. Plant Physiology. vol 1: 78-79.
10- Khalesro Sh., Ghalavand A., Sefidkon F., and Asgharzadeh A. 2012. The effect of biological and organic inputs on quantity and quality of essential oil and some elements content of anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants. 27(4): 551-560.
11- Kokkini s., Karousou D. and Vokou D. 1994. Pattern of geographic variation of organum Vulgari trichumes and essential oil content in Greece. Biochem syst Ecol. 22: 517-528.
12- Malakooti M. 1996. Sustainable agriculture and increase application performance optimization in Iran. Publication of Agricultural Education. 377-379.
13- Mehrafarin A., Nghdi badi H., Poorhadi M., Hadavi A., Ghavami N., and Kadkhoda Z. 2011. Phytochemical and field response of Peppermint (Mentha piperita L.) to use of bio-fertilizers and urea. Journal of Medicinal Plants. 10(4): 107-118.
14- Moradi R. 2009. Effects of biological and organic fertilizers on yield, yield components and the essential oil of fennel (Foeniculum vulgare). MS Thesis, Faculty of Agriculture, Ferdowsi University of Mashhad.
15- Moradi R., Rezvani Moghaddam P., Nasiri Mahallati M., and Nezhadali A. 2011 . Effects of organic and biological fertilizers on fruit yield and essential oil of sweet fennel (Foeniculum vulgare var. dulce). Spanish Journal of Agricultural Research. 9(2):546-553.
16- Niakan M., Khavari nezhad R., and Rezai M. 2004. Effect of Different ratios of three fertilizer N, P, K on fresh weight and dry weight, leaf area and plant essential oil of peppermint (Mentha piperita L.). Iranian Journal of Medicinal and Aromatic Plants. 20(2): 131-148.
17- Porra R., J., Thomposon W. A., and Kriedemann P. E. 1989. Determination of accurate extinction coefficients Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Photosynthesis Research. 975: 384-394.
18- Ratti N., Kumar S., Verma H.N. and Gautam S.P.2001. Improvement in bioavailability of tricalcium phosphate to Cymbopogon martinii var. motia by rhizobacteria, AMF and Azospirillum inoculation Microbiological Research, 156: 145-149.
19- Saleh rastin N. 1998. Biofertilizers. Journal of Water and Soil Sciences. 12(3): 1-36.
20- Salehi A., Ghalavand A., Sefidkon F., and Asgharzade A. 2011. The effect of zeolite, PGPR and vermicompost application on N, P, K concentration, essential oil content and yield in organic cultivation of German Chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants. 27(2):188-201.
21- Sedghi moghadam M., and Mirzai M. 2008. Effects of municipal solid waste compost on some quantitative and qualitative properties of pumpkin. Proceedings of the Third National Congress of recycling and use of renewable resources in organic farming. April. 2008.
22- Shah hoseini R., Omidbaigi R., and kiani D. 2012. Effect of Nitroxin and biosulfur bio-fertilizers and Super absorbent Polymers on growth, yield and quality of essential oil of basil herb. Journal of Horticultural Science. 26(3): 246-254.
23- Sharma A.K. 2002. Biofertilizers for Sustainable Agriculture Agrobios, India.300p.
24- Weisany W., Rahimzadeh S.,and Sohrabi Y. 2012. Effect of biofertilizers on morphological, physiological characteristic and essential oil content in basil (Ocimum basilicum L.). Iranian Journal of Medicinal and Aromatic Plants. 28(1): 73-87.
25- Zargari A. 1997. Medicinal Plants. vol 4: 71-75.
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