اثرات ضد باکتریایی ترکیبی اسانس کرفس کوهی) Kelussia odoratissima Mozaffarian) و کلپوره (L. Teucrium polium) با نانوذرات سنتزی نقره علیه باکتری‌های غذا زاد

نوع مقاله : مقالات پژوهشی

نویسندگان

• مجید عزیزی ¹
• منصور مشرقی ¹
• فاطمه عروجعلیان ²
• ناصر شاه طهماسبی ¹

¹ دانشگاه فردوسی مشهد

² دانشگاه تهران

https://doi.org/10.22067/jhorts4.v30i2.40634

چکیده

به منظور بررسی برهم‌کنش نانوذرات نقره با اسانس‌های گیاهان دارویی کرفس‌کوهی و کلپوره از نظر اثرات ضدباکتریایی، آزمایشی براساس محاسبه حداقل غلظت بازدارندگی (MIC) ترکیبی و تعیین شاخص FIC یا غلظت بازدارنده افتراقی و با روش ساده شده checkboard طراحی و به اجرا درآمد. اسانس گیاهان مورد بررسی به روش تقطیر با آب استخراج گردید. در این تحقیق باکتری‌های آلوده کننده مواد غذایی رایج مانند Staphylococcus aureus، Bacillus cereus Listeria monocytogenes، Escherichia coli O157:H7 و Salmonella enterica و Pseudomonas aureogenosa مورد استفاده قرار گرفتند. نتایج این تحقیق نشان داد که خاصیت ضدباکتریایی اسانس کلپوره قویتر(MIC بین 16/0 تا 25/1 میلی‌گرم در میلی‌لیتر) از اسانس کرفس‌کوهی (MIC بین 3/0 تا 5/2 میلی‌گرم در میلی لیتر) بود. خاصیت ضدباکتریایی نانوذرات نقره نیز (MIC بین 006/0 تا 025/0 میلی‌گرم در میلی‌لیتر) بسیار قابل توجه بود. محاسبه غلظت بازدارنده افتراقی (شاخص FIC) حاکی از وجود اثرات هم افزایی بین نانوذرات نقره و اسانس هر دو گیاه بود که بستگی به نوع پاتوژن مورد بررسی داشت. در نتیجه‌ی کاربرد توام نانوذرات نقره و اسانس این دو گیاه بویژه اسانس کلپوره با غلظت کمتر اسانس خاصیت بازدارندگی قابل توجهی بدست آمد. در هیچکدام از تیمارها برهم‌کنش آنتاگونیستی بین نانوذرات نقره و اسانس این دو گیاه مشاهده نشد.

کلیدواژه‌ها

• برهمکنش
• سینرژیست
• گیاهان دارویی
• نانوذرات نقره

20.1001.1.20084730.1395.30.2.9.6

عنوان مقاله [English]

Antibacterial Activities of Kelussia odoratissima and Teucrium polium Essential Oils in Combination of Synthetic Silver Nanoparticles against Food- borne Pathogens

نویسندگان [English]

• Majid Azizi ¹
• Mansour Mashreghi ¹
• Fatemeh Oroojalian ²
• Naser Shahyahmasebi ¹

¹ Ferdowsi University of Mashhad

² Tehran University

چکیده [English]

To study the interaction of silver nanoparticles with antibacterial effects of Kelussia odoratissima and Teucrium polium essential oils consolidated trial based on calculation of MIC and FIC index or differential inhibitory concentration and simple method checkboard designed and implemented. Essential oil of the plants extracted by hydro-distillation using clevenger apparatus. In this study, common food contaminant bacteria such as Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli O157: H7, Salmonella enterica and Ps. aureogenosa were used. The results showed that the antibacterial properties of the essential oil of Teucrium is stronger (MIC between 0.16 to 1.25 mg/ ml) than of the oil of Kelussia (MIC between 0.3 to 2.5 mg/ml). Antibacterial properties of silver nanoparticles (MIC between 0.006 to 0.025 mg) were stronger significantly. Calculate the differential inhibitory concentration index (FICi) implies the existence of synergistic and additive effect between silver nanoparticles and oil of both plant which will depend on the type of pathogen. This means that the combined use of silver nanoparticles and essential oil of the plant especially Teucrium essential oils strong inhibitory effect with lower concentrations of the essential oils was observed. Antagonistic interaction between silver nanoparticles and oil treatments in any of these two plants were observed.

کلیدواژه‌ها [English]

• Kelussia odoratissima
• antibacterial properties
• microdilution technique
• Silver Nanoparticle

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