Document Type : Research Article

Authors

Ferdowsi University of Mashhad

Abstract

Introduction: The ever-increasing tendency to the use of medicinal plants in the world has grown concerns about their cultivation and production processes. As medicinal plants are more compatible with the nature, special interest and attention have recently been given to herb therapy, and use of medicinal plants, being limited by the rise of pharmaceutical drugs, has become again common and widespread due to a number of reasons. In a sustainable agriculture system, application of the fertilizers which are nature friendly and suitable for plants is essential. This becomes more important when dealing with medicinal plants. Doing studies over the effect of nano‌pharmax and humic acid fertilizer on the plant, no research findings were obtained. So, in order to use less chemical fertilizers to prevent environmental pollution and encourage farmers to use more organic fertilizer, the present study was carried out to evaluate the effect of foliar application of humic acid and nano-pharmax fertilizer on the growth index, yield, yield components, essential oil content of N. sativa.
Materials and Methods: The experiment was conducted at Research Station, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, in 2012-2013. Land preparation was done in October. The land area was 80 square meters in which three blocks were designed. Eight plots were prepared within each block and each plot was planted with 4 lines. Furrow sowing operations were carried out on October 29. The plants were thinned in 4-6 leaf stage. First irrigation after planting, and subsequent ones were done every 7 days until the end of the growing season. No herbicides were applied in this plan and weeding was done by hand. The test treatments included humic acid and nano‌pharmax fertilizer with the levels of 0, 1, 3 and 6 mg per liter and 0 and 1 ml per liter, respectively. Fertilizer treatments was applied at the 8-10 leaf stage and continued once every two weeks, three times until after flowering. Foliar application continued until the plants were well treated with the solution. Plant height, leaf area index, dry weight, the number of branches, and the number of capsules per plant, the number of seeds per capsule, seed weight, seed yield, biological yield, essential oil content and yield were measured. The factorial experiment was conducted in a completely randomized block design with three replications. For the variance analysis of test data and drawing graphs, Excel and Minitab-16 software was used. All the averages data were compared at the 5 and 1% level, according to LSD test.
Results and Discussion: Different levels of humic acid imposed a significant effect on plant height, leaf area index, dry weight, the number of branches, and the number of capsules per plant, the number of seeds per capsule, seed weight, seed yield, biological yield, essential oil content and yield. Nano fertilizer application significantly increased the yield and essential oil content of Nigella sativa. Combined treatment at various levels had significant effect on dry weight, seed weight, biological yield and essential oil content and yield. According to the results, it can be concluded that the levels of 6 mg.l-1 of humic acid and Nano fertilizer (Farmks®) application the treatments were more effective in the increase of growth index, yield, yield components, essential oil content on other traits. The increased seed yield affected by humic acid and nano‌ fertilizer can be attributed to the better vegetative growth, canopy development and consequently, more appropriate use of solar radiation and high photosynthesis. Using nano ‌fertilizer, the time and speed of nutrients dispersion is coordinated by food requirements of the plant and thus, the plant will be able to absorb the maximum amount of nutrients and therefore, while reducing the leaching of nutrients and the crop yield increases.
Conclusion: The results showed that the use of nano fertilizer and humic acid can have positive effects on yield components and yield of N. sativa. Application of nano‌ fertilizer and humic acid concentrations of 6 mg/l showed higher yield than other treatments. Intensifying the vegetative growth, these treatments increased the yield in N. sativa species. The combined treatments significantly increased the seed weight, growth index, seed yield, biological yield essential oil content and yield. Therefore, on the basis of the results of current study, it sounds like that nano‌ fertilizer and humic acid can reduce the use of chemical fertilizers and environmental pollution. They also play an important role in achieving the goals of sustainable agriculture.

Keywords

1-Albayrak S., and Camas N. 2005. Effect of different levels and application times of humic acid on root and leafyield and yield component of Forage turnip (Brassica rapaL.). Journal of Agronomy, 42: 130-133.
2-Amuamuha L.,PirzadA.,andHadi H. 2012.Effect of varying concentrations and time of nanoiron foliar application on the yield and essential oil of pot Marigold(Calendula officinalis L).Journal of Applied and Basic Sciences, 3(10): 2085-2090.
3- Arancon Q., Edwards C.A., Lee S., and Byrne R. 2006. Effects of humic acids from vermicomposts on plant growth.European Journal of Soil Biology, 42: 865-869.
4-Ayas H., and Gulser F. 2005.The effect of sulfur and humic acid on yield components and macronutrient contents of spinach (SpinaciaoleraceaL.).Journal of biological sciences, 5(6): 801- 804.
5-Azarpou A. 2012.Evaluation and determination of the best time of priming and priming solution levels for germination indexes of fenugreek (TrigonellafoenumgracumL.).Journal of Agricultural and Biological Science, 7(3):141-146.
6- Balakumbahan R., and Rajamani K. 2010.Effectof bio stimulants on growth and yield of Senna(Cassia angustifolia L.).Journal of Horticultural Sciences, 2(1): 16-18.
7- Befrozfar M.R., Habibi D., Asgharzadeh A., Sadeghi- Shoae M., and Tookalloo M.R. 2013. Vermicompost, plant growth promoting bacteria and humic acid can affect the growth and essence of sweet basil(OcimumbasilicumL.). Annals of Biological Research, 4(2): 8-12.
8-Baghayi N., Keshavarzan N., and Nazaran M.M. 2011.Effect of Khazranano-chelated iron fertilizer on yield and yield components of rice (Oryza sativa L.).1th National Conference on Modern Topicsin Agriculture.
9-Bozorgi H.M. 2013.Study effects of nitrogen fertilizer management under nao iron chelate foliar spraying on yield components of eggplant(SolanummelongenaL.). Journal of Food Science and Technology 5(4): 398-403.
10- Cacilia R., Juarez R., Craker L., and Mendoza A. 2011. Humic substances and moisture content in the production of biomass and bioactive constituents of Thyme (Thymus vulgaris.L.).Articulocientifico, 34(3):183-188.
11-Chen Y., Clapp C.E., and Magen H. 2004. Mechanisms of plant growth stimulation by humic substances: The role of organo-iron complexes. SoilScience and Plant Nutrition,50:1089-1095.
12-Cu H., Sun C., Liu Q., Jiang J., and Gu W. 2006. Applications of nanotechnology in agrochemical formulation, perspectives, challenges and strategies. P. 1-6. Institute of Environment and Sustainable Development in Agriculture.Chinese Academy of Agricultural Sciences. Beijing. Chin.
13- Derosa M.R., Monreal C, Schnitzer M., Walsh R., and Sultan Y. 2010.Nanotechnology in fertilizers.NatureNanotechnol, 5:91.
14-Davazdahemami S.,andMajnoonhossein N.2008. Cultivation and production of some medicinal and spice plants. Tehran University Publication.300 pages.
15- El-Ghamry A., Kamar M., El- Hai A., and Khalid G. 2009. Amino and humic acids promote growth, yield and disease resistance of Broad bean (Faba vulgaris L. ) cultivated in clayey soil.Australian Journal of Basic and Applied Sciences, 3(2): 731-739.
16-Griffe P., Metha S., and Shankar D. 2003. Organic production of medicinal, aromatic and dye-yielding plants (MADPs): Forward, Preface and Introduction, FAO.
17-Ghorbani S., Khazayi H.M, Kafi M., Banayanaval M.2010.Effect of Humic acid in irrigation water on yield and yield components of maize(Zea mays L.).Journal of Ecology, 2(1):131-123.(In Persian with English abstract).
18-Hassanpouraghdam M.B., Tabatabaie S.J., Nazemiyeh H. and Aflatuni A. 2008. N and nutrition levels affect growth and essential oil content of costmary (Tanacetumbalsamita L.). Food, Agriculture and Environment, 6(2): 150 -154.
19-Khazaie H.R., EyshiRezaie E., and Bannayan M. 2011. Application times and concentration of humic acid impact on above ground biomass and oil production of hyssop (Hyssopusofficinalis). Medicinal Plants Research, 5(20):5148-5154.
20- Keshavarz N., BaghayiN.,andGhafari M. 2011.Effect of foliar applications of chelated iron nano-organic fertilizer on quantitative and qualitative properties of dryl and wheat(TriticumaestivumL.).1thNational Conference on Modern opicsinAgriculture.SavehIslamic Azad University.
21-Khoramdel S., kuchaki A., NasiriMahalati M., and Ghorbani R. 2008.Effect of biofertilizers on growth indices of black cumin (Nigella sativa L.).Journal ofAgriculture Research,6:294-285.(In Persian with English abstract).
22- Monreal C.M. 2010. Nanofertilizers for Increased N and P Use Efficiencies by Crops. p. 12-13. In summary of information currently provided to MRI concerning applications for Round of the Ontario Research Fund-Research Excellence program.
23- Musazadeh M., Baradaran R., and Seghatoleslami M.M. 2009. Investigation of density and spraying time effects on theyield and yield components and harvest index of black cumin(NigellasativaL.).Journal of Agriculture Research,8:42-48. (In Persian with English abstract).
24- Nazaran M.H., Khalaj H., Labafi M.R., Shamsabadi M., andRazaziA.2009.Investigation of iron chelatednano-organic fertilizer spraying time effect on quantitative and qualitative dryl and wheat(TriticumaestivumL.).Second National Conference on Application of Nanotechnology in Agriculture.Seed and Plant Improvement Institute. Karaj.16th to 15th October.
25- NazariM., Mehrafarin A., NaghdibadiH., andKhalighi-sigaroodiF. 2012.Morphological traits of sweet basil(OcimumbasilumL.) as influenced by foliar application of methanol nano-iron chelate fertilizers.Annals of Biological Research, 3 (12):5511-5514.
26- Omidbaigi R. 2009. Production and processing of medicinal plant (3 rdedition.Vol. 1).RazaviGhodsAstan Publication.347 pp.
27- Prasad T.N., Sudhakar P., Sreenivasulu Y., Latha P., Munaswamy V., Raja Reddy K., Sreeprasad T.S., Sajanlal P.R., and Pradeep T. 2012. Effect of nanoscales Zinc Oxide on the germination, growth and yield of peanut(ArachishypogaeaL).Journal of Plant Nutrition, 35: 905-912.
28- Shaalan M.N. 2005. Influence of biofertilizers and chicken manure on growth, yield and seeds quality ofblack cumin(Nigella sativa L.) plants. Egyptian Journal of Agricultural Research, 83:811-828.
29-Shaban S.H.A., Manal F., and Afifi H.M.N. 2009. Humicacid Foliar application to Minimize soil applied fertilization of surface- irrigated Wheat(TriticumaestivumL.).Journal of Agricultural science,5(2):207-210.
30-Soumare M.F., Tack M.G., and Verloo M.G. 2003.Effect of a municipal solid waste compost and mineral fertilization on plant growth in two tropical agricultural soils of Mali. Bioresource Technology, 86: 15-20.
31-Terzi A., Coban S., Yildiz F., Ates M., Bitiren M., Taskin A., and Aksoy N. 2010. Protective effects of black cumin(Nigella sativaL.)on intestinal ischemia-reperfusion injury in rats, Journal of Investigative Surgery, 23(1): 21-27.
32- Zhang L., Hong F., Lu S., and Liu C. 2005. Effect of nano TiO2 on strength of naturally aged seeds and growth of spinach (SpinaciaeracealoL.).Biological Trace Element Research 105: 83–9.
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