Effects of Foliar Application of Nano Zinc Chelate and Zinc Sulfate on Zinc Content, Pigments and Photosynthetic Indices of Holy Basil (Ocimum sanctum(

Document Type : Research Article

Authors

• Zohreh Moghimi pour ¹
• Mohammad Mahmoodi Sourestani ¹
• Naser Alemzadeh Ansari ²

¹ Shahid Chamran University of Ahvaz

² Shahid Chamran University of Ahva

https://doi.org/10.22067/jhorts4.v30i2.39348

Abstract

Introduction: Holy basil is a perennial plant belongs to Lamiaceae family. The plant is a perennial and thrives well in the hot and humid climate. Its aerial parts have been in use for food, pharmaceuticals, cosmetics and perfumery industries. Leaves contain 0.5-1.5% essential oil and main oil components are eugenol, methyl eugenol, carvacrol, methyl chavicol and1,8-cineole. A balanced fertilization program with macro and micronutrients is very important in producing high quality yield. Zinc is involved in IAA production, chlorophyll biosynthesis, carbon assimilation, saccharids accumulation, reactive oxygen radicals scavenging and finally carbon utilization in volatile oil biosynthesis.
Material and methods: In order to evaluate the effect on zinc foliar application on zinc content of leaves, photosynthetic indices and pigments of holy basil, an experiment was carried out in 2013 at a research farm of Horticultural Science, Shahid Chamran University (31°20'N latitude and 48°40'E longitude and 22.5 m mean sea level), Ahvaz (Iran), a region characterized by semi-dry climate. The experiment was arranged based on Randomized Complete Block Design (RCBD) with six treatments and three replications. The treatments were nano zinc chelate (0, 0.5, 1 and 1.5 g.l-1) and zinc sulfate (1 and 1.5 g.l-1) fertilizers. Land preparation includes disking and the formation of raising beds (15cm high and 45cm wide across the top) using a press-pan-type bed shaper. Holy basil seeds were sown on two rows on each bed, with 15 cm in-row and 40 cm between-row spacing. The plants were irrigated weekly as needed. Foliar application of zinc fertilizers was done at six-eight leaf stage and were repeated with interval 15 days until full bloom stage. Zinc content, stomata conductance (gs), CO2 under stomata (Ci), transpiration rate (E), net photosynthesis (Pn), light use efficiency (LUE), water use efficiency (WUE) and also chlorophyll a, chlorophyll b, chlorophyll a+b and carotenoid contents were measured at beginning of flowering stage. Photosynthetic parameters were measured by Infra-red gas analyzer (LCA4, ADC Co. Ltd., Hoddesdon, UK). Instantaneous water use efficiency (WUEinst) was calculated as Pn/E ratio. Light use efficiency was calculated as Pn/PPFD ratio.
Result and discussion: The results showed that the effect of foliar application of zinc fertilizers on all measured traits except Ci and WUE was significant (p≥0.01). The highest values of zinc content (110.53 mg.kg-1), chlorophyll a (0.99 mg.kg-1 fresh weight), chlorophyll b (0.30 mg.kg-1 fresh weight), chlorophyll a+b (1.29 mg.kg-1 fresh weight) and carotenoid (0.18 mg.kg-1 fresh weight) traits were obtained in plants sprayed with 1.5 g.l-1 nano zinc chelate. The lowest amount of zinc content (21.37 mg.kg-1), chlorophyll a (0.58 mg.kg-1 fresh weight), chlorophyll b (0.14 mg.kg-1 fresh weight), chlorophyll a+b (0.72 mg.kg-1 fresh weight) and carotenoid (0.13 mg.kg-1 fresh weight) traits were obtained in control plants. Foliar application of holy basil with 1.5 g.l-1 nano zinc chelate led to increase in stomata conductance (322.22 mm H2O.m-2.s-1), transpiration rate (2.86 mm H2O.m-2.s-1), net photosynthesis (11.75 μm CO2.m-2.s-1) and light use efficiency (6.10 μm CO2. μm photon-1). The minimum amount of stomata conductance (172.00 mm H2O.m-2.s-1), transpiration rate (2.16 mm H2O.m-2.s-1), net photosynthesis (8.23 μm CO2.m-2.s-1) and light use efficiency (4.46 μm CO2. μm photon-1) were observed in control plants. There were positive correlation (p≥0.01) between zinc content and chlorophyll a, chlorophyll b, chlorophyll a+b and carotenoid. Zinc content also had positive and significant correlation (p≥0.01) with stomata conductance, CO2 under stomata, transpiration rate, net photosynthesis and light use efficiency. So, providing zinc by foliar application with 1 and 1.5 g.l-1 nano zinc chelate and 1.5 g.l-1 zinc sulfate can lead to increase in chlorophyll and carotenoid contents. Increase in net photosynthesis may be due to higher photosynthesis pigments and also stomata conductance and CO2 under stomata. In the other hand, zinc is an essential micronutrient that acts either as a metal component of various enzymes or as a functional, structural, or regulatory cofactor such as carbonic anhydrase, ribulose 1, 5-bisphosphate carboxylase/oxygenase and fructose-1, 6-bisphosphate, and is thus associated with saccharide metabolism and photosynthesis.
Conclusion: Although the highest amount of most measured traits was obtained in plants that treated with 1.5 g.l-1 nano zinc chelate, there were no significant difference between 1 and 1.5 g.l-1 nano zinc chelate and 1.5 g.l-1 zinc sulfate treatments for zinc content, stomata conductance, CO2 under stomata, transpiration rate, net photosynthesis, water use efficiency and light use efficiency. Therefore, in order to increase zinc content and photosynthetic traits of holy basil, foliar application of with 1.5 g.l-1 zinc sulfate is recommended.

Keywords

• Carotenoid
• Chlorophyll
• Holy basil
• net photosynthesis
• stomatal conductance

20.1001.1.20084730.1395.30.2.10.7

References

1- Arnon D.I. 1989. Copper enzymes in isolated chloroplasts, polyphenoloxidase in Beta vulgaris, Plant Physiology, 24: 1-15.

2- Auld D.S. 2001. Zinc coordination sphere in biochemical zinc sites, Biometals, 14: 271–313.

3- Azizi G., Alimardani, L. and Syahmarghay A. 2011. The relationship between the number on chlorophyll content meter with chlorophyll content, photosynthesis and leaf nitrogen content in soybean (Glycine max), Journal of Plant Science Research, 23 (3): 34-40. (in Persian)

4- Derakhshani Z., Hassani A., Sadaghiani M.H.R., Hassanpouraghdam M.B., Khalifani, H. and Dalkani M. 2011. Effect of zinc application on growth and some biochemical characteristics of costmary (Chrysanthemum balsamita), Communications in Soil Science and Plant Analysis, 42 (20): 2493-2503.

5- Derakhshani Z., Hassani A., Sefidkon F., Rasouli Sadaghiani M.H., Hassanpouraghdam, M.B. and Gheibi S.A. 2011.Effects of soil and foliar application of zinc on some growth characteristics and essential oils of costmary (Tanacetum Balsamita L.), Journal of Horticultural Science and Technology, 12 (2): 99-103. (in Persian)

6- Farahat M.M., SoadIbrahim M.M., Lobna, S. and El-Quesni F. 2007. Response of vegetative growth and some chemical constituents of Cupressus sempervirens to foliar application of ascorbic acid and zinc at Nubaria, Soil Science, 3(4): 496-500.

7- Fateh H., Siosemardeh, A.M. and Sharafi S. 2012. Effect of drought stress on photosynthesis and physiological characteristics of barley, International Journal of Farming and Allied Sciences, 1(2): 33-42.

8- Gohari G., Hassanpouraghdam M., Dadpour, M. and Shirdel M. 2013.Influence of Zn foliar application on growth characteristics and essential oil yield of basil (Ocimum basilicum L.) under salinity stress, Journal of Science and Technology of Greenhouse Culture.4 (15):15-24. (In Persian with English abstract)

9- Hasnaa S., Ayad, F. and Abdalla M.S.A. 2010. Effect of putrescine and zinc on vegetative growth, photosynthetic pigments, lipid peroxidation and essential oil content of Geranium (Pelargonium graveolens), World Journal of Agricultural Science, 6(5): 601-608.

10- Heidari F., Zehtab Salmasi S., Javanshir A., Aliari, H. and Dadpoor M.R. 2008. The effects of application microelements and plant density on yield and essential oil of peppermint (Mentha piperita L.), Iranian Journal of Medicinal and Aromatic Plants, 24 (1): 1-9. (in Persian).

11- Kaya C., and Higgs D. 2002. Response of Tomato (Lycopersicon esculentum L) culture at low zinc, Scientia Horticulture, 93: 53–64.

12- Khalifa R.K.M., Shaaban, S.H.A., and Rawia A. 2011. Effect of foliar application of zinc sulfate and boric acid on growth, yield and chemical constituents of Iris plants, Ozean Journal of Applied Sciences, 4(2): 1-16.

13- Kumar K., Rao K.V., and Rao N.V. 1988. Studies on growth and activity of photosynthetic enzymes in Sorghum bicolor as influenced by micronutrients, Indian National Science Academy, 54(1): 54-79.

14- Kumar P., Rupesh Kumar M., Kavitha K., Jagadeesh S., and Rawoof Kh. 2012. Pharmacological actions of Ocimum sanctum, Indian Perfumer, 1(3): 404-416.

15- Ma X., Lee J.G., Deng, Y. and Kolmakov A. 2012. Interactions between engineered nanoparticles and plants: Phytotoxicity, uptake and accumulation, Science of the Total Environment, 408: 3053–3061.

16- Malakouti M.J., and Tehrani M.M. 2007. The Role of Microelements in Enhancing Performance and Improving the Quality of Agricultural Products. 3 th Edi. Tarbiat Modares University Pub., Tehran. (in Persian)

17- Marscher H. 2011. Mineral Nutrition of High Plant. 3th ed. Academic press.

18- Misra A., and Sharma S. 1991. Critical Zn concentration for essential oil yield and menthol concentration of Japanese mint, Nutrient Cycling in Agroecosystems, 29(3): 261-263.

19- Misra A., Dwivedi S.A., Srivastava K.D., Tewari K., Khan A., and Kumar R. 2007. Analysis of growth, physiology, photosynthesis, essential monoterpene oil(s) yield and quality in Ocimum sanctum L genotypes, Bioscience Research, 4(1): 01-05.

20- Nahed G.A., and Balbaa L.K. 2007. Influence of tyrosine and zinc on growth, flowering and chemical constituents of Salvia farinacea, Journal of Applied Sciences Research, 3(11): 1479-1481.

21- Norvell W.A., and Lindsy W.L. 1969. Reaction of EDTA coplexes of Fe, Zn, Mn and Cu with soils, Soil Science, 33: 86-91.

22- Omidbaigi R. 2005. Production and Processing of Medicinal Plant.Vol. 2 ed. 4.Astan Quds Razavi Pub. Mashad. (in Persian)

23- Peyvandi M., Parande, H. and Mirza M. 2011. Comparison of nano Fe chelate with Fe chelate effect on growth parameters and antioxidant enzymes activity of Ocimum basilicum, New Cellular and Molecular Biotechnology Journal, 1 (4):89-98. (in Persian)

24- Pirzad A.R., Tousi P., and Darvishzadeh R. 2013. Effect of Fe and Zn foliar application on plant characteristics and essential oil content of anise (Pimpinella anisum L.), Iranian Journal of Crop Sciences, 15(1): 12-23. (in Persian with English abstract)

25- Plank C.O. 1989. Plant Analysis Handbook for Georgia. University of Georgia Cooperative Extension Services, pp: 60-64.

26- Powell S.R. 2000. The antioxidant properties of zinc, Journal of Nutrition, 130: 1447-1449.

27- Prasad T., and Sudhakar P. 2012. Effect of nanoscale zinc oxide particles on the germination, growth and yield of Peanut (Arachis hypogaea), Journal of Plant Nutrition, 35: 905–927.

28- Rezaei R., Hosseini S.M., Shabanali Fami H., and Safa L. 2010. Identification and analysis of barriers to development of nanotechnology in agriculture, Science and Technology Policy, 2 (1): 17-26. (in Persian)

29- Said Al-Ahl H. and Mahmoud A. 2010. Effect of zinc and iron foliar application on growth and essential oil of sweet basil (Ocimum basilicum) under salt stress, Ozean Journal of Applied Sciences, 3(1): 97-111.

30- Sharmal P.N., Tripathi, A. and Bisht S.S. 1995. Zinc requirement for stomatal opening in Cauliflower. Plant Physiology, 107: 751-754.

31- Singh V., Amdekar S., and Verma O. 2011. Ocimum sanctum bio-pharmacological activities, Webmedcentral Pharmacology, 1(10): 1–7.

32- Weisany W., Rahimzadeh S., and Sohrabi Y. 2012. Effect of biofertilizers on morphological, physiological characteristic and essential oil content in basil (Ocimum basilicum L.), Iranian Journal of Medicinal and Aromatic Plants, 28 (1): 73-87. (in Persian with English abstract)