تأثیر شوری بر جوانه‌زنی و رشد گیاهچه در توده‌های بومی گراس فسکویه بلند در ایران

نوع مقاله : مقالات پژوهشی

نویسندگان

• آزاده موسوی بزاز ¹
• علی تهرانی فر ¹
• محمد کافی ¹
• علی گزانچیان ²
• محمود شور ¹

¹ دانشگاه فردوسی مشهد

² مرکز تحقیقات و منابع طبیعی خراسان رضوی

https://doi.org/10.22067/jhorts4.v0i0.33997

چکیده

نیاز به گراس‌های متحمل به شوری در حال افزایش است. رشد سریع جمعیت شهر نشین فشار عمدهای را به منابع آب شیرین وارد ساخته است. به همین منظور جهت بررسی تاثیر تنش شوری بر جوانه‌زنی و رشد گیاهچه توده‌های مختلف فسکویه بلند (Festuca arundinacea) آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با 12 ژنوتیپ فسکویه بلند از مناطق اصفهان (یزد آباد)، کامیاران، یاسوج، داران، سناجان، بروجن، مشهد، سمیرم، گندمان (نصیرآباد)، سنندج و البرز و فسکویه بلند وارداتی به عنوان فاکتور اول و چهار سطح شوری 0، 45، 90 و 135 میلی‌مولار Nacl به عنوان فاکتور دوم با چهار تکرار انجام شد. نتایج نشان داد که افزایش سطوح شوری موجب کاهش معنی‌دار درصد جوانه‌زنی، سرعت جوانه‌زنی، طول ریشهچه، طول ساقهچه وشاخصبنیه بذر در تمام ژنوتیپ ها شد. همچنین، اثر متقابل شوریو ژنوتیپ نیز برای صفات سرعت و درصد جوانه زنی و بنیه بذر معنی‌دار بود.اکثر گراس‌های مورد مطالعه در این آزمایش شوری تا 45 میلی‌مولار را بدون آن‌که مولفه‌های جوانه‌زنی به طور قابل ملاحظه‌ای تحت تاثیر قرار بگیرند را تحمل نمودند. توده‌های مشهد و بروجن در سطح شوری 135 میلی‌مولار بیشترین میزان را در صفات درصد و سرعت جوانه‌زنی نشان دادند. همچنین، در مجموع صفات جوانه‌زنی، توده‌های داران و مشهد نسبت به سایرین کمترین کاهش را داشته و می‌توانند به عنوان ژنوتیپ‌های متحمل به شوری در مرحله جوانه‌زنی پیشنهاد گردند.

کلیدواژه‌ها

• بنیه بذر
• سرعت جوانه‌زنی
• طول ریشه‌چه
• طول ساقه‌چه

20.1001.1.20084730.1394.29.2.12.2

عنوان مقاله [English]

Effect of Salinity on Seed Germination and Seedling Growth of Native Populations of Tall Fescue in Iran

نویسندگان [English]

• Azadeh Mousavi Bazaz ¹
• Ali Tehranifar ¹
• Mohammad Kafi ¹
• Ali Gazanchian ²
• Mahmood Shoor ¹

¹ Ferdowsi University of Mashhad

² Natural Resources Research Center of Khorassan

چکیده [English]

Introduction: Worldwide, more than one-third of irrigated land is salinized, and in many regions, fresh water shortage has resulted in restrictions on the use of potable water for landscape irrigation. On the other hand, rapidly expanding population growth is occurring in many arid regions, where soil and water salinity are problems and there are increased demands on limited fresh water resources (9). In the turf grass industry, with the increased use of saline and non-potable water, the development of turf grass landscapes in arid and seashore regions where saline soil is common, and with the use of salt for deicing roadways, the need for salinity tolerant turf grasses is very important (16).Seed germination and early seedling growth is usually the most critical stage in plant establishment, and determining successful crop production (23). Tall fescue (Festuca arundinacea Schrub.) is an important perennial cool-season grass in temperate regions and it is widely used for both forage and turf purposes (25). There is no study on the evaluation of salinity on tall fescue native populations in Iran. The major objective of this study is to determine the relative salt tolerance and growth response of native populations of tall fescue to salinity in germination stage.
Materials and Methods: In this experiment, seeds of some native populations of tall fescue (TF) (F. arundinacea Schreb) including: Semirom, Mashhad, Sanandaj, Sanajan, Yasuj, Yazd Abad, Daran, Kamyaran, Gandoman, Borujen, Nasir Abad, Alborz and commercial TF (C. TF) seeds were used. Four replicates of 25 seeds were germinated on filter papers with 5 ml of NaCl concentrations placed in 9 cm Petri dishes. NaCl concentrations included: 0, 45, 90 and 135 milimolar. The Petri dishes were transferred to germinator at 23°C.Germinated seeds were counted on the3rd, 5th, 8th, 11th and 14th days. Germination was considered to have occurred when the root length was 2 mm long. The seedling with short, thick, and spiral formed hypocotyls and stunted primary root were considered as abnormally germinated. Then, the total germination percentage, germination rate, root length and shoot length were calculated on Day 15. The vigor index was calculated as VI= (RL+SL)×GP, where for the VI, RL is the root length, SL is the shoot length and GP is the germination percentage. Root length and shoot length were measured manually with a ruler. The experimental design was a completely randomized design with 4 replications and 25 seed per replicate. The data were statistically analyzed by JMP 8.0. The difference between the means was compared using LSD values (P < 0.01).
Results and Discussion: The results indicated that the increase of salinity level leads to a significant decrease in germination percentage, germination rate, length of shoot, root and vigor index in all genotypes. Also, interaction of salinity and genotype was significant for germination percentage, germination rate, and vigor index. The highest germination percentage was related to Mashhad population at 45 milimolar, and Daran population at 90 and 135 milimolar. Also, the lowest germination percentage was related to Kamyaran population at 45 and 90 milimolar and Commercial tall fescue at 135 milimolar. Mashhad population at 135 milimolar, Daran population at 90 and 135 milimolar had the highest germination rates. The lowest germination rate was recorded at 45, 90 and 135 milimolar in Commercial tall fescue, Kamyaran and Sanandaj populations, respectively. The highest root length was seen in Commercial tall fescue at 90 and 135 milimolar, and Mashhad population at 45 milimolar. For shoot length factor, Mashhad population, Commercial tall fescue and Mashhad population had the highest lengths at 135, 90 and 45 milimolar, respectively. The highest vigor index was related to Mashhad, Daran and Mashhad populations, at 45, 90 and 135 milimolar, respectively. NaCl has an inhibitory effect on seedgermination and its effect on germination showed time course dependence for absorption of Na and Cl by thehypocotyls (28).Increasing salinity levels caused delays in seedlingemergence as a result of reducing cell division and plant growth metabolism (28). The negative effect of salinity on seed germination and early growth could be due to the toxic effects of NaCl on seeds, or to the osmotic effect, that prevents the seeds from imbibitions (21).
Conclusion: Major grasses mentioned above could tolerate 45 milimolar salinity without noticeable changes in germination traits. Mashhad and Brujen populations were least affected by 135 Mm Nacl at germination rate and percentage. Also, Daran and Mashhad populations were least affected by different salinity levels in all germination factors compared to other populations and could be suggested as salt-tolerant genotypes at germination stage.

کلیدواژه‌ها [English]

• Germination rate
• Length of root
• Length of shoot
• Vigor index

مراجع

1- Baldwin J.C., and Dombrowski J.E. 2006. Evaluation of Lolium temulentum as a model grass species for the study of salinity stress by PCR-based subtractive suppression hybridization analysis. Plant Science, 171: 459–469.

2- Bybordi A., and Tabatabaei J. 2009. Effect of salinity stress on germination and seedling properties in canola cultivars (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37: 71-76.

3- Goncalo A., de Souza F., Ferreira B.S., Dias J.M., Queiroz K.S., Branco A.T., ressan-Smith R. E., Oliveira J.G., and Garcia A.B. 2003. Accumulation of salt protein in rice plants as a response to environmental stresses. Plant Science, 164: 623-628.

4- ISTA. 1996. Rules for Seed Testing. International Seed Testing Association. Seed Science Technology, Zurich, Switzerland.

5- Khaleghi E., and Moallemi N. 2009. Effect of different levels of salinity and temperature on seed germination of Cocks Comb (Celosia argentea). Journal. of Plant Production, 16 (1): 149-163. (in Persian with English abstract)

6- Khosh Kholgh Sima N. A. 1999. Physiological aspects of fodder production on salt affected soils. PhD Thesis.

7- Khosh Kholgh Sima N. A., Ali Tabar R., Eghbali Nezhad M., Babazadeh P., and Tale Ahmad S. 2013. Effect of salinity on seed germination and tolerance threshold in Barley. Iranian Journal of Field Crops Research, 11: 107-120. (in Persian)

8- Khosh-Khui M. 2005. Plant propagation: Principle and practices. Shiraz University, shiraz. (in Persian)

9- Marcum K.B. 2006. Use of saline and non-potable water in the turfgrass industry: Constraints and developments. Agriculture water management, 80: 132-146.

10- Mohamadian S.M., Arzani A., and Rezaei A.M. 2011. The Effect of Salinity on Seed Germination and Seedling Growth of Triticale Genotypes. Journal of Crop Production and Processing, 1: 35-50. (in Persian with English abstract)

11- Mohammadi S., Khosh Kholgh Sima N.A., Majidi Heravan A., Noor Mohammadi Gh., and Saeedi A. 2004. Response of wheat genotypes to salinity during germination. Journal of Agricultural science, 14:88-105. (in Persian).

12- Mostafavi K., and Heydarian A. 2012. Effects of different salinity levels on germination indices in four sunflower varieties. Journal of Agronomy and Plant Breeding, 8: 123-131. (in Persian)

13- Noor E., Azhar. F. M., and Khan A. L. 2001. Differences in responses of gossypium hirsutum L. varieties to NaClsalinity at seedling stage. International Journal of Agriculture and Biology, 3: 345-347.

14- Oksu G., Kaya M. D., and Atak M. 2005. Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turkish Journal of Agriculture and Forestry, 29: 237-242.

15- Pahlavani M.H., Saeidi G., and Mirlohi A.F. 2006. Estimates of genetic parameters for seed germination of safflower in different salinity levels. Asian Journal of Plant Science, 5: 133–138.

16- Qian Y.L., Engelke M.C., and Foster M.J.V. 2000. Salinity effects on Zoysia grass cultivars and experimental lines. Crop Science, 40:488-492.

17- Riahinia Sh., Khazaee H.M., and Razmjoo Kh. 2011. Effect of salinity on germination and seedling growth of populations of turfgrasses. Iranian Journal of Field Crops Research, 9 (2): 222- 228. (in Persian).

18- Roberts E.C., and Zybura E.L. 1967. Effect of sodium chloride on grasses for roadside use. Highway Research Record, 193: 35-42.

19- Roohollahi I., Kafi M. Sayyad Amin P., and Arghavani M. 2008. Salinity effect on germination and Initial growth of Poa pratensis , Cynodon dactylon , Lolium perenne. Pajouhesh & Sazandegi, 81: 147-153. (in Persian with English abstract)

20- Rouhi H.R., Aboutalebian M.A., and Sharifzadeh F. 2011. Seed priming improves the germination traits of tall fescue (Festuca arundinacea). Notulae Scientia Biologicae, 3: 57-63.

21- Shakarami B., Dianati-Tilaki Gh., Tabari M., and Behtari B. 2011. The effect of priming treatments on salinity tolerance of Festuca arundinacea Schreb and Festuca ovina L. seeds during germination and early growth. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 18 (2): 318-328. (in Persian with English abstract)

22- Sherma M. L.1973. Simulation of drought and its effect on germination of five pasture species. Agronomy Journal, 65: 982-987.

23- Soltani A., Ghalipoor M. and Zeinali E. 2006. Seed reserve utilization and seedling of wheat as affected by drought and salinity. Journal of Environmental and Experimental Botany, 55: 195-200.

24- Taghipour F., and Salehi M. 2008. The study of salt tolerance of Iranian barley (Hordeum vulgar) genotypes in seedling growth stages. American-Eurasian Journal of Agricultural and Environmental Science, 1: 53-58.

25- Tehran parks and green space organization. 2005. Lawning principles. Tehran parks and green space organization. (in Persian).

26- Tester M., and Davenport R. 2003. Na+ tolerance and Na+ transport in higher plants. Annual Botany, 91: 503–527.

27- Tlig T., Gorai M., and Neffati M. 2008. Germination responses of Diplotaxis harra to temperature and salinity. Flora, 203: 421-428.

28- Turhan, H., and Ayaz C. 2004. Effect of salinity on seedling emergence and growth of sunflower(Helianthus annuus.) cultivars. International Journal of Agricultur and Biology, 6: 149–152.

29- Wahome P.K., Jesch H.H., and Grittner I. 2001. Mechanisms of salt stress tolerance in two rose rootstocks: Rosa chinensis `Major' and R. rubiginosa. Scientia Horticulturae, 87: 207-216.

30- Yildirim E., and Guvenc I. 2006. Salt tolerance of pepper cultivars during germination and seedling growth. Turkish Journal of Agriculture and Forestry, 30: 347-353.

31- Zia S., and Ajmal KhanM.2004. Effect of light, salinity and temperature onseed. Canadian Journal Botany, 82: 151-157.