بررسی ترکیبات فیتوشیمیایی عصاره‌های اتانولی و اتیل استاتی سه اکوتیپ گیاه دارویی درمنه کوهی (Artemisia aucheri)

نوع مقاله : مقالات پژوهشی

نویسندگان

• سپیده هوشمند ¹
• سعیده علیزاده سالطه ¹
• صاحبعلی بلندنظر ¹
• الیاس آریاکیا ²

¹ گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

² عضو هیأت علمی بانک گیاهی، مرکز ملی ذخایر ژنتیکی و زیستی ایران (IBRC) (ACECR)

https://doi.org/10.22067/jhs.2024.80951.1233

چکیده

از جمله گیاهان با ارزش ایران، گونه‌های جنس Artemisia متعلق به تیره کاسنی (Asteraceae) می‌باشند که پراکندگی نسبتاً وسیعی در نقاط مختلفی از ایران دارند. با توجه به اینکه پتانسیل آنتی‌اکسیدانی به‌میزان قابل توجهی با ماهیت حلال، روش استخراج گیاه جمع‌آوری ‌شده از هر منطقه و نیز بستگی به پارامترهای زیادی از جمله آب‌وهوا، ارتفاع و نور دارد. در این مطالعه، گیاه دارویی درمنه کوهی با نام علمی Artemisia aucheri از سه ناحیه مختلف ایران (سمنان، مازندران و اصفهان) در سال 1400 جمع‌آوری و پتانسیل آنتی‌اکسیدانی این اکوتیپ‌ها ارزیابی شد. در این تحقیق، خواص دارویی از جمله محتوای آنتی‌اکسیدانی (با دو روش DPPH و FRAP)، مقدار فنل کل و فلاونوئید در عصاره‌های قطبی (اتانول) و غیرقطبی (اتیل استات) و درصد و تنوع ترکیبات اسانس مورد ارزیابی قرار گرفت. اکوتیپ سمنان دارای بیشترین فعالیت آنتی‌اکسیدان (95/15 درصد) می‌باشد. حلال غیرقطبی اتیل استات فعالیت احیاکنندگی بالاتری را نشان داده که این مورد بیانگر این است که نوع حلال مورد استفاده در عصاره‌گیری بر فعالیت آنتی‌اکسیدانی بسیار مؤثر می‌باشد. اکوتیپ اصفهان دارای بیشترین محتوای فنلی در عصاره قطبی (62/62 میلی‌گرم معادل اسید گالیک بر گرم وزن خشک) و اکوتیپ مازندران بیشترین محتوای فلاونوئیدی را در عصاره غیرقطبی (8/52 میلی‌گرم معادل کورستین بر گرم وزن خشک) نشان داد. اکوتیپ سمنان دارای بیشترین ترکیبات مونوترپن اکسیژن‌دار، بیشترین ترکیب در اسانس را در بین دیگر اکوتیپ‌ها دارا بود. این نتایج بیانگر این مورد هستند که گونه A. aucheri دارای منابع غنی آنتی‌اکسیدان‌های طبیعی بوده و می‌توانند در صنایع غذایی و دارویی کاربرد فراوان داشته باشند. تنوع محتویات فنلی، ترکیبات اسانس و آنتی‌اکسیدانی مشاهده شده در سه اکوتیپ مورد مطالعه می‌تواند به‌علت عوامل مختلف اکولوژیکی، ژنتیکی، جغرافیایی و فاکتورهای تغذیه‌ای باشد.

کلیدواژه‌ها

• آرتمیزیا
• DPPH
• فنل
• عصاره قطبی
• عصاره غیر قطبی

موضوعات

• گیاهان دارویی

عنوان مقاله [English]

Studying the Phytochemical Compounds of Ethanol and Ethyl Acetate Extracts of Three Ecotypes of Artemisia aucheri

نویسندگان [English]

• Sepideh Houshmand ¹
• Saeideh Alizade ¹
• Sahebali Bolandnazar ¹
• Elyas Aryakia ²

¹ Department of Horticultural Science and Engineering, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

² Department of Plant Bank, Iranian Biological Resource Center (IBRC)(ACECR), Tehran, Iran

چکیده [English]

Introduction
Taking into account Iran's unique meteorological and biological characteristics due to its geographic position. This has contributed to the variety and abundance of plant species cultivated there. The Artemisia species, which are among Iran's most valuable plants, are members of the Asteraceae family and are found across the country in reasonably large numbers. Antioxidants now play an indisputable role in the food, pharmaceutical, and healthcare industries. Given that the antioxidant capability is greatly influenced by the kind of solvent used, the technique used to extract the plants that were harvested from each location, as well as other factors like the weather, altitude, and light. The substantial antioxidant activity of phenolic and flavonoid compounds and their protective significance in cancer illnesses are caused by these compounds' regenerative properties.
 
Materials and Methods
In this work, Artemisia aucheri, a medicinal plant, was gathered from Semnan, Mazandaran, and Isfahan in Iran, and the antioxidant activity of these ecotypes was assessed. In this study, the quantity of total phenol and flavonoids in polar (ethanol) and non-polar (ethyl acetate) extracts, as well as the proportion and diversity of essential oil components, were assessed. Antioxidant content was also determined using the DPPH and FRAP techniques.
 
Results and Discussion
The most active antioxidant is found in the Semnan ecotype. The polar solvent of ethanol showed the strongest inhibition whereas the non-polar solvent of ethyl acetate shown stronger reducing activity, proving the importance of the extraction solvent on antioxidant activity in various processes. The non-polar extract (ethyl acetate) from the Mazandaran ecotype had the greatest flavonoid concentration, while the polar extract (ethanol) from the Isfahan ecotype had the highest phenolic content. The ethanolic extract performed the best when assessing total phenol. The most crucial elements of essential oils are oxidized monoterpenes. Oxygenated monoterpenes are present in 54.82% of the Semnan ecotype, 38.81% of the Mazandaran ecotype, and 24.41% of the Isfahan ecotype. In comparison to other ecotypes, the Semnan ecotype exhibited the most oxygenated monoterpene compounds and the greatest number of essential oil-containing compounds.
 
 
Conclusion
These findings suggest that A. aucheri possesses abundant natural antioxidant sources and is useful in both the food and pharmaceutical industries. A key aspect is the act of extraction, which is focused on the extraction's goal. The solvent used during extraction significantly affects the outcomes. Regarding the chemical makeup of the compounds, the solvent's polarity directly affects the solutes that are extracted. Since phenolic chemicals are more attracted to polar solvents, they are found in plant samples. The kind of flavonoids found in plants and their degree of polarity determine the variation in the quantity of flavonoid content between polar and non-polar extracts. On the other hand, a variety of ecological, genetic, regional, and dietary variables may have contributed to the variation in phenolic contents, essential oils, and antioxidant chemicals found in the three analyzed ecotypes.

کلیدواژه‌ها [English]

• Artemisia
• DPPH
• Non-polar extract
• Phenol
• Polar extract

©2024 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

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