بررسی تنوع مورفولوژیکی و فیتوشیمیایی جمعیت‌های مختلف گوش‌بره (Phlomis olivieri Benth.) در استان همدان

نوع مقاله : مقالات پژوهشی

نویسندگان

• مهتاب صالحی ¹
• رمضان کلوندی ²

¹ گروه علوم و مهندسی باغبانی، دانشگاه بوعلی سینا، مجتمع آموزش عالی نهاوند (ویژه دختران)، نهاوند، ایران

² بخش تحقیقات منابع طبیعی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان همدان، سازمان تحقیقات، آموزش و ترویج کشاورزی، همدان، ایران

https://doi.org/10.22067/jhs.2022.74320.1117

چکیده

جنس Phlomis از تیرۀ Lamiaceae حدود 113 گونه را در جهان شامل می­شود. براساس آخرین گزارش، جنس Phlomis دارای 20 گونه و 3 هیبرید در ایران است که 9 گونۀ آن، انحصاری ایران هستند. گیاه Phlomis olivieri Benth. از گونه­های بومی ایران است. گونه­های مختلف Phlomis در طب سنتی، برای درمان برخی بیماری­ها ازجمله زخم معده، دیابت و التهاب مورد استفاده قرار گرفته است. این پژوهش، به ‌منظور ارزیابی تنوع مورفولوژیکی و فیتوشیمیایی 11 جمعیت گیاه گوش­بره، در سال 1400 در مناطق مختلف استان همدان به اجرا درآمد. براساس نتایج به دست آمده، بیشترین وزن تر و وزن خشک سرشاخه گل­دار (96/6 و 48/3 گرم) و همچنین بیشترین وزن تر و وزن خشک گیاه (به ترتیب 77/11 و 86/5 گرم) به جمعیت کوهانی تعلق داشت. بلندترین طول گل­آذین (2/24 سانتی‌متر) مربوط به جمعیت جوزان و کوتاه­ترین طول گل­آذین (9/8 سانتی‌متر)  مربوط به جمعیت گاماسیاب بود. بیشترین قطر ساقه (45/4 میلی­متر) در جمعیت گرین مشاهده شد. بیشترین ارتفاع گیاه (4/49 سانتی‌متر) مربوط به جمعیت راهدارخانه بود که با جمعیت‌ گرین، تفاوت معنی‌داری نداشت (p<0.05) و کمترینِ آن، مربوط به جمعیت گرمک (31 سانتی‌متر) بود که با جمعیت گاماسیاب (50/33 سانتی­متر) اختلاف معنی­داری نداشت. همچنین، 31 ترکیب در اسانس شناسایی شد که کاریوفیلن، ژرماکرن دی، و ای ـ بتا ـ فارنسن، بیشترین درصد اجزای تشکیل­دهنده اسانس را به خود اختصاص دادند. در این مطالعه، بیشترین اسانس (04/0 درصد) از منطقه کوهانی به دست آمد که در بین جمعیت­های مورد مطالعه، دارای کمترین ارتفاع از سطح دریا بود. بنابراین به نظر می­رسد که عوامل محیطی نیز همانند عوامل ژنتیکی، در ایجاد تنوع در خصوصیات مورفولوژیکی و فیتوشیمیایی این گیاه مؤثر بوده­اند.

کلیدواژه‌ها

• ارتفاع از سطح دریا
• اسانس
• سرشاخه گلدار
• گل آذین

20.1001.1.20084730.1401.36.3.14.5

موضوعات

• گیاهان دارویی

عنوان مقاله [English]

Evaluation of Morphological and Phytochemical Diversity of different Populations of Phlomis olivieri Benth. in Hamedan Province

نویسندگان [English]

• Mahtab Salehi ¹
• Ramezan Kalvandi ²

¹ Horticultural Sciences and Engineering Department, Nahavand Higher Education Complex, Bu-Ali Sina University, Nahavand, Iran

² Natural Resources Research Division, Hamedan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Hamedan, Iran

چکیده [English]

Introduction
 Medicinal plants have played an essential role in the development of human culture. Medicinal plants are resources of new drugs and many of the modern medicines are produced indirectly from plants. Although the production of secondary metabolites is controlled by genes, their production is considerably influenced by environmental conditions, so environmental factors cause changes in the growth of medicinal plants as well as the amount of active substances. Essential oil quantity and quality are affected by the different environmental conditions. Physiological, morphological and genetic variations were seen in populations of species that occurred in different habitats. These variations were created in response to contrasting environmental conditions. In many plant species, studies on the pattern of variation in populations have shown the localized populations are adapted to the particular environmental conditions of their habitat. The genus Phlomis L. (Lamiaceae) includes about 113 perennial herbs or shrubs distributed in Asia, Europe, and Africa. Some of the Phlomis species have been reported for their traditional uses as analgesic, diuretic, tonic, anti-diarrheic agents and to treat various conditions such as gastric ulcer, inflammation, diabetes, hemorrhoids and wounds. In Flora of Iran, this genus is represented by 20 species, including Phlomis olivieri Benth.
Materials and Methods
 This study was conducted to evaluate the morphological and phytochemical diversity of eleven populations of P. olivieri Benth. from different districts of Hamedan province in 2021. Traits such as plant height, stem diameter, leaf length and width, fresh and dry weight of the flowering branch, inflorescence length, fresh and dry weight of the plant, number of inflorescence cycles, essential oil percentage were measured. In order to investigate the physical and chemical properties of soil, soil samples were collected from a depth of 30 cm. Then they were transferred to the soil laboratory. Plants samples were collected in the flowering stage and were dried at 25-30°C. They were stored in envelopes at 22±3°C away from the sun. For extracting the essential oil of the samples, 100 gr of the plant was milled and then distilled with water. Hydrodistillation lasted for 4 hours. The main components of essential oil were identified and determined by gas chromatography in the Institute of Medicinal Plants in Karaj. Gas chromatography was carried out on Agilent 6890 with capillary column 30m*0.25 mm, 0.25 mm film thickness. The grouping of populations based on morphological and phytochemical traits was done by cluster analysis in SPSS using the Ward method. Also, the traits correlation (quantitative) was done using the Pearson method.
Results and Discussion
 According to the results, the highest fresh and dry weight of flowering branch (6.96 g and 3.48 g) and also the highest fresh and dry weight of the plant (11.77 g and 5.86 g) belonged to the Koohani population. The tallest inflorescence (24.2 cm) belonged to the Jowzan population and the shortest inflorescence belonged to the Gammasiab population (8.9 cm). The highest stem diameter (4.45 mm) was observed in the Garin population. The maximum plant height (49.4 cm) was related to the Rahdarkhaneh population, which was not significantly different from the Garin population, and the minimum was related to the population of Garmak (31 cm), which was not significantly different from the population of Gammasiab (33.50 cm). Also, 31 compounds were identified in this plant essential oil that caryophyllene, germacrene D, and (E)-b-Farnesene had the highest percentage of essential oil constituents. In this study, the highest amount of essential oil (0.04%) was related to the Koohani population which had the lowest altitude among other populations. Therefore, it seems that environmental factors, as well as genetic factors, have been effective in creating diversity in morphological and phytochemical characteristics of this plant.
Conclusion
 The results obtained from this study showed that P. olivieri Benth. populations gathered from different regions of Hamedan province, had a high diversity in terms of essential oil content. The results showed that in addition to genetic factors, environmental and climatic factors also affect phytochemical traits. In this study, the highest amount of essential oil was produced in the Koohani population (located in Nahavand city) with the lowest altitude among other populations. According to the research on the essential oil components of P. olivieri in different regions of Iran, the components of its essential oil and their percentages are completely different; so, some of the components that are seen in one region, are not observed in another region, and this issue emphasizes on the effect of climatic conditions. This difference is quite evident even in the studied populations in a province.
 

کلیدواژه‌ها [English]

• Altitude
• Essential oil
• Flowering branch
• Inflorescence

مراجع

1.  

   1. Adams, R.P. (2001). Identification of essential oil components by gas chromatography/mass spectroscopy. Allured Publishing Corporation, Carol Stream, Australia.
   2. Alibakhshi, M., Mahdavi, S.Kh., Mahmoodi, J., & Ghelichnia, H. (2014). Phytochemical evaluation of Stachys inflata essential oil in different habitats of Mazandaran Province. Eco-phytochemical Journal of Medical Plants 2(2): 56-68. (In Persian) dor: 20.1001.1.23223235.1393.2.2.7.7.
   3. Alimohammadi, M., Yadegari, M., & Shirmardi, H.A. (2017). Effect of elevation and phonological stages on essential oil composition of Stachys. Turkish Journal of Biochemistry (Turk Biokimya Dergisi) 42(6): 647-656. https://doi.org/10.1515/tjb-2016-0267.
   4. Argyropoulou, C., Daferera, D., Tarantilis, P.A., Fasseas, C., & Polissiou, M. (2007). Chemical composition of the essential oil from leaves of Lippia citriodora H.B.K. (Verbenaceae) at two developmental stages. Biochemical Systematics and Ecology 35(12): 831-837. https://doi.org/10.1016/j.bse.2007.07.001.
   5. Azarnivand, H., Ghavam Arabani, M., Sefidkon, F., & Tavili, A. (2010). The effect of ecological characteristics on quality and quantity of the essential oils of Achillea millefolium L. subsp. millefolium. Iranian Journal of Medicinal and Aromatic Plants 25(4): 556-571. (In Persian with English abstract). https://doi.org/10.22092/ijmapr.2010.7141.
   6. Azizi, A., Hadian, J., Gholami, M., Friedt, W., & Honermeier, B. (2012). Correlations between genetic, morphological, and chemical diversities in a germplasm collection of the medicinal plant Origanum vulgare L. Chemistry and Biodiversity 9(12): 2784-2801. https://doi.org/10.1002/cbdv.201200125.
   7. Bigdeloo, M. (2012). Evaluation of morphological, genetic and phytochemical diversity of Thymus carmanicus. MSc. Thesis. Tehran University, 114p. (In Persian with English abstract)
   8. Bigdeloo, M., Nazeri, V., & Hadian, J. (2013). Study on effect of some environmental factors on morphological traits and essential oil productivity of Thymus caramanicus Jalas. Iranian Journal of Medicinal and Aromatic Plants 28(4): 756-766. (In Persian with English abstract). https://doi.org/10.22092/ijmapr.2013.2930.
   9. Chehregani, A., Mohsenzadeh, F., Mirazi, N., Hajisadeghian, S., & Baghali, Z. (2010). Chemical composition and antibacterial activity of essential oils of Tripleurospermum disciforme in three developmental stages. Pharmaceutical Biology 48(11): 1280-1284. https://doi.org/10.3109/13880201003770143.
   10. Crock, J., Wildung, M., & Croteau, R. (1997). Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-β-farnesene. PNAS (Proceedings of the National Academy of Sciences of the United States of America) 94(24): 12833-12838. https://doi.org/10.1073/pnas.94.24.12833.
   11. Fathalipoor, Z., Nabati Ahmadi, D., Rajabi Memari, H., Siyahpoosh, A., & Seddighi Dehkordi, F. (2014). Determination of plant variation using morphological properties and cluster analysis in Anethum germplasms. Journal of Plant Productions (Agronomy, Breeding and Horticulture) 37(4): 57-67. (In Persian)
   12. Ghasemi Dehkordi, N. (2002). Iranian herbal pharmacopoeia. Vol. 1, p18-21.
   13. Ghasemi Pirbalouti, A., Momeni, M., & Bahmani, M. (2013). Ethnobotanical study of medicinal plants used by Kurd tribe in Dehloran and Abdanan districts, Ilam province, Iran. African Journal of Traditional, Complementary and Alternative Medicines 10(2): 368-385. https://doi.org/10.4314/ajtcam.v10i2.24.
   14. Jamzad, Z. (2012). Flora of Iran: Lamiaceae. Research Institute of Forests and Rangelands, Tehran, p. 263-264. (In Persian)
   15. Khalilzadeh, M.A., Rustaiyan, A., Masoudi, Sh., & Tajbakhsh, M. (2005). Essential oils of Phlomis persica Boiss. and Phlomis olivieri Benth. from Iran. Journal of Essential Oil Research 17(6): 624-625. https://doi.org/10.1080/10412905.2005.9699014.
   16. Khorshidi, J., Shokrpour, M., & Nazeri, V. (2020). Assessment of morphological diversity among different populations of Thymus daenensis Celak. Journal of Plant Research (Iranian Journal of Biology) 33(3): 593-606. (In Persian with English abstract). dor: 20.1001.1.23832592.1399.33.3.8.2.
   17. Koike, T., Kitao, M., Quoreshi, A.M., & Matsuura, Y. (2003). Growth characteristics of root-shoot relations of three birch seedlings raised under different water regimes. Plant and Soil 255: 303-310. https://doi.org/10.1023/a:1026199402085.
   18. McLafferty, F.W., & Stauffer, D.B. (1989). Wiley / NBS Registry of Mass Spectral Data. 7 Volume Set. Wiley, New York.
   19. Mirza, M., & Nik, Z.B. (2003). Volatile constituents of Phlomis olivieri Benth. from Iran. Flavour and Fragrance Journal 18(2): 131-132. https://doi.org/10.1002/ffj.1156.
   20. Mohammadifar, F., Delnavazi, M.R., & Yassa, N. (2015). Chemical analysis and toxicity screening of Phlomis olivieri Benth. and Phlomis persica Boiss. essential oils. Pharmaceutical Sciences 21(1): 12-17. https://doi.org/10.15171/PS.2015.11.
   21. Montanari, R.M., Barbosa, L.C.A., Demuner, A.J., Silva, C.J., Carvalho, L.S., & Andrade, N.J. (2011). Chemical composition and antibacterial activity of essential oils from verbenaceae species: alternative sources of (E)-caryophyllene and germacrene-D. Química Nova 34(9): 1550-1555. https://doi.org/10.1590/S0100-40422011000900013.
   22. Mozaffarian, V. (2015). Identification of medicinal and aromatic plants of Iran. 2^nd edition, Farhang Moaser Publishers, Tehran, Iran, p. 540-543.
   23. Nemeth, E., & Bernath, J. (2008). Biological activities of yarrow species (Achillea spp.). Current Pharmaceutical Design 14(29): 3151-3167. https://doi.org/10.2174/138161208786404281.
   24. Omidbaigi, R. (2013). Approaches of production and products of medicinal plants. Vol. 1, 7^th edition, Tehran, Tarrahan-e-Nashr, p. 154. (In Persian)
   25. Sadeghi, F., Aboli, J., & Ali-Asgari, S. (2013). Chemical decomposition of essential oil of Phlomis olivieri Benth. by gas chromatography - mass spectrometry. Journal of Quantum Chemistry and Spectroscopy (JQCS) 3(8): 45-51. (In Persian)
   26. Salehi, M., & Kalvandi, R. (2020). Evaluation of morphological and phytochemical characteristics changes in different populations of Stachys inflata Benth. in Hamedan province. Journal of Horticultural Science 34(2): 247-260. (In Persian with English abstract). https://doi.org/10.22067/jhorts4.v34i2.79307.
   27. Santos-Gomes, P.C., Fernandes-Ferreira, M., & Vicente, A.M.S. (2005). Composition of the essential oils from flowers and leaves of Vervain [Aloysia triphylla (L'Herit.) Britton] grown in Portugal. Journal of Essential Oil Research 17(1): 73-78. https://doi.org/10.1080/10412905.2005.9698835.
   28. Sarkhail, P., Abdollahi, M., & Shafiee, A. (2003). Antinociceptive effect of Phlomis olivieri Benth., Phlomis anisodonta Boiss. and Phlomis persica Boiss. total extracts. Pharmacological Research 48(3): 263-266. https://doi.org/10.1016/S1043-6618(03)00151-8.
   29. Sarkhail, P., Amin, G., & Shafiee, A. (2006). Composition of the essential oil of Phlomis olivieri Benth. from north of Iran. DARU Journal of Pharmaceutical Sciences 14(2): 71-74.
   30. Schepetkin, I.A., Özek, G., Özek, T., Kirpotina, L.N., Khlebnikov, A.I., & Quinn, M.T. (2020). Chemical composition and immunomodulatory activity of Hypericum perforatum essential oils. Biomolecules 10(6): 916. https://doi.org/10.3390/biom10060916.
   31. Shahbazi Asl, F., & Jafari, A.A. (2020). Investigation of aerial part yield and morphological traits in populations of Mentha pulegium. Iranian Medicinal Plants Technology 3(1): 29-39. (In Persian with English abstract). https://doi.org/10.22092/mpt.2020.342777.1062.
   32. Statistical Center of Iran. (2012). Statistical yearbook of Hamedan province, Chapter 1. (In Persian)
   33. Sun, Y., Qiao, H., Ling, Y., Yang, S., Rui, C., Pelosi, P., & Yang, X. 2011. New analogues of (E)-β-farnesene with insecticidal activity and binding affinity to aphid odorant-binding proteins. Journal of Agricultural and Food Chemistry 59(6): 2456-2461. https://doi.org/10.1021/jf104712c.
   34. Tajbakhsh, M., Rineh, A., & Khalilzadeh, M.A. (2007). Chemical composition of the essential oils from leaves, flowers, stem and root of Phlomis olivieri Benth. Journal of Essential Oil Research 19: 501-503. https://doi.org/10.1080/10412905.2007.9699315.
   35. Vaezi, J., Behroozian, M., Joharchi, M.R., & Memariani, F. (2017). Phlomis iranica (Lamiaceae: Lamioideae), a new species from Khorassan-Kopet Dagh floristic province, NE Iran. Turkish Journal of Botany 41: 392-403. https://doi.org/10.3906/bot-1608-38.