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نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 دانشگاه ارومیه

چکیده

شوری یکی از فاکتورهای مهم محیطی است که رشد و نمو گیاهان را تحت تاثیر قرار داده و تولید گیاهان را محدود می‌کند. سدیم نیترو پروسید (SNP) به طور معمول به عنوان ترکیب رها کننده نیتریک اکسید (NO) در گیاهان استفاده می‌شود. نیتریک اکسید رادیکال گازی نسبتاً پایداری است که در غلظت‌های پایین با ممانعت از تولید رادیکال‌های فعال اکسیژن مانع خسارت آن‌ها می‌گردد. این پژوهش به صورت فاکتوریل با طرح کاملاً تصادفی در سه تکرار، تیمار کلرید سدیم در پنج سطح صفر (شاهد)، 25، 50، 75 و 100 میلی‌مولار و سدیم نیتروپروسید به صورت محلول پاشی برگی درچهار غلظت صفر (شاهد)، 25/0، 5/0 و 75/0 میلی‌مولار انجام شد. بنابر نتایج بدست آمده در این پژوهش، تنش شوری باعث کاهش رشد رویشی گردید و با افزایش غلظت نمک، بیشتر ویژگی‌های مورفولوژیک گیاه تحت تاثیر منفی تنش قرار گرفتند. تنش شوری، نیز دارای اثرات منفی قابل توجهی بر ویژگی‌های فیزیولوژیکی بود به گونه‌ای که باعث کاهش کلروفیل شد و از طرفی باعث افزایش ظرفیت آنتی‌اکسیدانی، پرولین، قند محلول و نشت الکترولیت در گیاه گردید. تیمارهای مختلف سدیم نیتروپروسید توانست به طور معنی‌داری اثر منفی شوری به ویژه در سطوح پایین شوری بر گیاه را کاهش دهد. در بین غلظت‌های مختلف سدیم نیتروپروسید، غلظت 25/0 و 5/0 میلی‌مولار بر روی بهبود صفات مورفولوژیکی، غلظت های 25/0، 5/0 و 75/0 میلی‌مولار بر روی بهبود صفات فیزیولوژیکی و غلظت‌های 5/0 و 75/0 میلی مولار بر روی بهبود صفات بیوشیمیایی موثر واقع شدند.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation on the Protective Role of Nitric Oxide in Reducing Damages Induced by Salinity Stress in Calendula officinalis L.

نویسندگان [English]

  • Maryam Jabbarzadeh 1
  • َAli Tehranifar 1
  • Jafar Amiri 2
  • Bahram Abedy 1

1 Ferdowsi University of Mashhad

2 Urmia University

چکیده [English]

Introduction: Salinity is one of the most important environmental factors that regulates plant growth and development, and limits plant production. Researchers have shown that some plant growth regulators such as nitric oxide improve the plants resistance to environmental stresses such as heat, cold, drought and salinity. Sodium nitroprusside (SNP) commonly has been used as nitric oxide (NO) donor in plants. NO is a diffusible gaseous free radical. Low concentrations of NO inhibit the production of reactive oxygen species and protect plants against ROS damages. The aim of this study was to evaluate the role of SNP as NO donor on salt tolerance of Calendula officinalis and its effects on some morphological, physiological and biochemical characteristics of this plant.
Materials and Methods: In this study, the effects of salinity (0, 25, 50, 75 and 100 mM) and sodium nitroprusside (0.0, 0.25, 0.50 and 0.75 mM) on morphological and physiological characteristics of Calendula officinalis L. were investigated. Total leaf area and number of leaves were determined in the end of the experiment. Electrolyte leakage was used to asses’ membrane permeability. This procedure was based on Lutts et al.,1995. Soluble sugars were extracted and estimated by the method of Irigoyen et al., 1992. Chlorophyll a, b and carotenoid content were calculated from the absorbance of extract at 653, 666 and 470 nm using the formula of Dere et al., 1998. Proline was extracted by the method of Bates et al., 1973. DPPH radical- scavenging activity of sample was performed as described previously of Cleep et al., 2012. The SAS software was used for the analysis of variance (ANOVA), comparisons with P

کلیدواژه‌ها [English]

  • Proline
  • Reactive oxygen species
  • Sodium nitroprusside
  • Soluble sugars
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