Effects of Organic, Chemical and Biological Fertilizers on Crude Protein, Oil Yields and Fatty Acids of Black Seed (Nigella sativa L.)

Document Type : Research Article

Authors

• Parviz Rezvani Moghaddam
• Seyyed Mohammad Seyyedi

Ferdowsi University of Mashhad

https://doi.org/10.22067/jhorts4.v0i0.28253

Abstract

In order to investigate the effects of organic, chemical and biological fertilizers on crude protein and oil yields and fatty acids composition in black seed (Nigella sativa L.), a field experiment was conducted at Agricultural Research Station of Ferdowsi University of Mashhad, Iran, in 2009-2010. The experiment was arranged by using a complete randomized block design based on factorial arrangement with three replications and 12 treatments. The experimental treatments included fertilizer sources (vermi compost, urea fertilizer and control) as first factor and different biological fertilizers (nitroxin (including Azotobacter sp. and Azospirillum sp), mycorrhizae, biosulfur (including Thiobacillus sp.) + sulfur and control (no biofertilizer)) as second factor. Results showed that crude protein and oil yields of black seed in vermi compost were significantly higher than urea fertilizer. In addition, the biological fertilizer had no significant increasing effects on crude protein and oil yields, except biosulfur + sulfur. Chemical analysis of black seeds showed a composition of 10.9% crude protein and 24.5% fat. Linoleic (49.18%) and oleic acids (26.77%) was the major unsaturated while palmitic acid (12.68%) was the main saturated fatty acid.

Keywords

• unsaturated fatty acids
• Oil percentage
• Gas chromatography mass

20.1001.1.20084730.1394.29.1.14.2

References

1- خرم دل س.، کوچکی ع.، نصیری محلاتی م. و قربانی ر. 1387. اثر کاربرد کودهای بیولوژیک بر شاخص های رشدی سیاهدانه (Nigella sativa L.). مجله پژوهش های زراعی ایران 6: 294-285.

2- خندان ا. و آستارایی ع. 1384. تاثیر کودهای آلی (کمپوست زباله شهری، کود گاوی) و شیمیایی بر برخی خصوصیات فیزیکی خاک. بیابان 10: 368-361.

3- خواجه پور م.ر. 1385. گیاهان صنعتی. انتشارات جهاد دانشگاهی واحد صنعتی اصفهان. 564 صفحه.

4- رضوانی مقدم پ.، سیدی س.م. 1392. مقایسه تاثیر منابع آلی، شیمیایی و بیولوژیک نیتروژن بر کارایی مصرف نیتروژن در سیاهدانه (Nigella sativa L.). فصل نامه تحقیقات گیاهان دارویی و معطر ایران 3: در دست چاپ.

5- سجادی نیک ر.، یدوی ع.، بلوچی ح.ر. و فرجی ه. 1390. مقایسه تأثیر کودهای شیمیایی (اوره)، آلی (ورمی کمپوست) و زیستی (نیتروکسین) بر عملکرد کمی و کیفی کنجد (Sesamum indicum L.). نشریه دانش کشاورزی و تولید پایدار، 21: 101- 87.

6- سیدی س.م.، رضوانی مقدم پ.، قربانی ر. و نصیری محلاتی م. 1391. اثر دوره بحرانی رقابت علف های هرز بر عملکرد، اجزای عملکرد و عملکرد روغن سیاهدانه (Nigella sativa L.). نشریه علوم باغبانی 26: 122-113.

7- فروغی فر ح. و پورکاسمانی م.ا. 1381. علوم و مدیریت خاک (جلد اول) (ترجمه). انتشارات دانشگاه فردوسی مشهد. 336 صفحه.

8- محمدی آریا م.، لکزیان ا. و حق نیا غ. 1389. تاثیر مایه تلقیحی حاوی باکتری تیوباسیلوس و قارچ آسپرژیلوس بر رشد گیاه ذرت. مجله پژوهش های زراعی ایران 8: 89-82.

9- AI-Jassir M.S. 1992. Chemical composition and microflora of black cumin (Nigella sativa L.) seeds growing in Saudi Arabia. Food Chemistry, 45: 239-242.

10- AOCS: Official Methods and Recommended Practices of the American Oil Chemists Society. 1993. AOCS Press, Champaign, II. (USA).

11- Atta M.B. 2003. Some characteristics of nigella (Nigella sativa L.) seed cultivated in Egypt and its lipid profile. Food Chemistry, 83: 63-68.

12- Erkan N., Ayranci G., and Ayranci E. 2008. Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmaric acid and sesamol. Food Chemistry, 110: 76-82.

13- Farhoosh R., Haddad Khodaparast M.H., and Sharif A. 2009. Bene hull oil as a highly stable and antioxidative vegetable oil. Eurepian Journal of Lipid Science and Technology, 111:1259-1265.

14- Hussain A., Nadeem A., Ashraf I., and Awan M. 2009. Effect of weed competition periods on the growth and yield of black seed (Nigella sativa L.). Pakistan Journal of Weed Science Research, 15: 71-81.

15- Khattak K.F., Simpson T.J., and Hasnullah I. 2008. Effect of gamma irridation on the extraction yield, total phenolic content and free radical-scavengingactivity of Nigella sativa seed. Food Chemistry, 110: 967-972.

16- Mehta B.K., Pandit V., and Gupta M. 2009. New principle from seeds of Nigella sativa. Natural Product Research, 23: 138-148.

17- Nergiz C., and Otles S. 1993. Chemical composition of Nigella sativa L. seeds. Food Chemistry, 48: 259-261.

18- Nickavar B., Mojab F., Javidnia K., and Roodgar Amoli M.A. 2003. Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. Z. Naturforsch, 58: 629-631.

19- Ramadan M.F., and Morsel J.T. 2003. Analysis of glycolipids from black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.) and niger (Guizotia abyssinica Cass.) oilseeds. Food Chemistry, 80: 197-204.

20- Rodriguez Caceres E.A., Gonzalez Anta G., Lopez J.R., Di Ciocco C.A., Pacheco Basurco J.C., and Parada J.L. 1996. Response of field-grown wheat to inoculation with Azospirillum brasilense and Bacillus polymyxa in the semiarid region of Argentina. Arid Soil Research and Rehabilitation, 10:13-20.

21- Salem M.L., and Hossain M.S. 2000. Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection. International Journal of Immunopharmacology, 22: 729-740.

22- Salimpour S., Khavazi K., Nadian H., Besharati H., and Miransari M. 2010. Enhancing phosphorous availability to canola (Brassica napus L.) using P solubilizing and sulfur oxidizing bacteria. Australian Journal of Crop Science, 4: 330-334.