Optimizing of in vitro Zygotic Embryo Culture of Salvia leriifolia Benth.

Document Type : Research Article

Authors

• Masumeh Modarres ¹
• Mehrdad Lahouti
• Ali Ganjeali
• Javad Asili ²

¹ Shahid Hasheminejad Campus -Farhangian University of Mashhad

² Faculty of Pharmacy, Mashhad University of Medical Sciences

https://doi.org/10.22067/jhorts4.v0i0.42782

Abstract

Salvia leriifolia (Lamiaceae) is endemic of Khorasan and Semnan province and an endangered plant. Poor seed germination of this plant has a serious problem with the high production. The first step to improve this precious plant is to produce sterile plantlets in order to prepare explants of appropriate vigor. In this study,in vitro culture of Salvia leriifolia zygotic embryo was performed through a factorial experiment in the form of completely random design including culture medium, BAP and NAA. The results showed that, MS and 1/2MS media appeared to be more efficient than B5 medium and significant differences were observed. Efficient concentrations of BAP and NAA were 1mgL-1 and had a significant effect in growth and development of embryos. The seedlings were obtained 10 days after planting. Based on these results, the best choice for quick access to strong seedlings, seedling growth and development of embryos is MS and 1/2MS media supplemented with 1mgL-1 BAP and NAA.

Keywords

• Optimizing
• Embryo culture
• Salvia leriifolia

20.1001.1.20084730.1393.28.3.6.1

References

1- پور ح.، نعمتی س.ح.، تهرانی‌فر ع.، شور م.، و جوهرچی م.ر. 1389. بررسی آرایش و عمق کشت بذر بر خصوصیات جوانه زنی و استقرار گیاهچه(Salvia leriifolia Benth.) به منظور اهلی سازی نوروزک. نشریه علوم باغبانی علوم و صنایع کشاورزی.24: 136-141.

2- تایز ل. و زایگر ا. 1386. فیزیولوژی گیاهی . ترجمه انجمن زیست شناسی ایران. انتشارات خانه زیست شناسی.تهران.

3- حدادخداپرست م.ح. و حسینی م. 1372. اثر عوامل محیطی بر جوانه زنی گیاه نوروزک در شرایط آزمایشگاهی. مجله پژوهش و سازندگی، 37: 45-42.

4- مدرس م.، ابریشم چی پ.، اجتهادی ح.، و رمضانی ع. 1386الف. تکثیر گیاه نوروزک با استفاده از کشت رویان. فصلنامه علمی پژوهشی تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران، 15: 141-129.

5- مدرس م.، ابریشم چی پ.، فرهوش ر.، و اجتهادی ح. 1386ب. بررسی تغییر فعالیت آنتی اکسیدانی ریشه و برگ نوروزک(Salvia leriifolia) در مراحل رشد و نمو. مجله علمی پژوهشی تحقیقات گیاهان دارویی و معطر ایران، 23: 294-285.

6- مدرس م.، و ابریشم‌چی پ. 1389. بررسی فعالیت ضد باکتریایی ریشه گیاه نوروزک (Salvia leriifolia)، در مراحل مختلف رشد و نمو ،مجله زیست شناسی ایران23 :717-707.

7- مدرس م.، و ابریشم‌چی پ. 1387 بررسی تأثیر زمان برداشت بر فعالیت ضد باکتریایی برگ گیاه نوروزک (Salvia leriifolia)، مجله علمی پژوهشی علوم دانشگاه تربیت معلم 8: 343-356.

8- Chin H.F., Krishnapillay B., and Alang Z.C. 1998. Media for embryo culture of some tropical recalcitrant species. Pertanika, 11: 357-363.

9- Craverol V., and Cointry E. 2007. Shortening the seed-to-seed cycle in artichoke breeding by embryo culture. Plant Breeding, 126: 222-224.

10- Hadad Khodaparast M.H., Haghdoost A., Elhami-Rad A.H., Movahhed, G., and Karazhiyan, H. 2006. Antioxidant activity and thermal Properties of salvia leriifolia (Norozak) root extract. Proceedings of the international conference on Innovations in Food and Bioprocess Technologies, 378.

11- Hosseinzadeh H., and Lary P. 2000. The effect of Salvia leriifolia Benth root extracts on morphine dependence in mice. Phytotherapy Research, 14:384-387.

12- Hosseinzadeh H., and Yavary M. 1999. Anti-inflammatory effect of Salvia leriifolia Benth leaf extract in mice and rat. Pharmaceutical and Pharmacological Letters, 9: 60-61.

13- Hosseinzadeh H., Haddad Khodaparast M.H., and Hosseini E. 2000.Anti-ulcer effect of Salvia leriifolia Benth leaf extract in mice. Pharmaceutical and Pharmacological Letters, 10:63-64.

14- Jalili A., and Jamzad Z. 1999. Red Data Book Of Iran .A Preliminary Survey of Endemic, Rare and Enudaugered Plants species in Iran. Research Institute of Forests and Rangelands (RIFR) Publication, Tehran,750 p.

15- Keiichi S., Tetsuya T., Fumio H., and Yusuke S. 2005. In vitro propagation of sterile mutant strains in Japanese morning glory by sub-culturing embryoids derived from an immature embryo. Journal of the Japanese Society for Horticultural Science, 74: 311-317.

16- Loizze M.R., Menchini F., Tundis R., Bonesi M., and Cenforti F. 2009. In vitro biological activity of Salvia leriifolia Benth essential oil relevant to the treatment of Alzheimer’s disease Journal of Oleo Science, 58: 443-446.

17- Misic D., Grubisic D. and Konjevic R. 2006. Micropropagation of Salvia brachyodon through nodal explants. Biologia Plantarum, 50: 473-476.

18- Ogunsola K.E., and Ilori C.O. 2008. In vitro propagation of miracle berry (Synsepalumdulcificum Daniel) through embryo and nodal cultures. African Journal of Biotechnology, 7: 244-248.

19- Rechinger K.H. 1982. Flora Iranica. N.150, Academishe Druk.u.Verlag sustalt Gratz.

20- Sanchez-Zamora M., Cos-terrer A., Frutos-Tomas D., and Garcia-Lopez R. 2006. Embryo germination and proliferation in vitro of Juglans regia L. Scientia Horticulture, 108: 317-321.

21- Thomas T.D. 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26: 618-631.

22- Toosi S., Dilmagani K., and Hecmatshoar H. 2010. Proliferation of Juglans regia L. by in vitro embryo culture. (JCBG)Journal of Cell Biology and Genetics, 1:13 - 20.

23- Zhao Z., Andersen S.U., Ljung K., Dolezal K., Miotk A., Schultheiss S.J., and Lohmann J.U. 2010. Hormonal control of the shoot stem-cell niche. Nature, 465: 1089-1092.