Document Type : Research Article

Authors

Department of Horticultural Sciences, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

Introduction
In recent years, the use of organic acids has increased due to their role in the quantitative and qualitative yield and resistance to environmental stresses. Ascorbic acid (vitamin C) is one of the important antioxidants and plays a role as the primary substrate in cyclic pathways to remove toxicity and neutralize superoxide and single oxygen radicals. Ascorbate is also involved in the regulation of cell division and photosynthesis and has nutritional value for humans and is probably important for the tolerance of plants against photo-oxidative stress. Oxalic acid is a metabolic end product in plants that has many physiological functions, the main ones is the induction of resistance to disease and environmental stress by increasing the activity of enzymes involved in resistance and secondary metabolites such as phenol, flavonoid, etc. Considering the importance of the physiological traits of the plant in the production of quantitative and qualitative yield of strawberry and on the other hand, the lack of sufficient information about the effect of external application of ascorbic acid and oxalic acid on the physiological traits of the plant, the present research work aimed to investigate some physiological and qualitative traits of strawberry leaves and fruits affected by foliar spraying of ascorbic acid and oxalic acid.
 
Materials and Methods
The experiment was conducted based on randomized complete block design (RCBD) with three replications in Darkalate village of Ramiyan city of Golestan province to investigate some physiological responses of strawberry cv. Camarosa to the foliar application of organic acids. The experimental treatments consisted of three levels: non-spraying as control, spraying with 1 mM ascorbic acid and 1 mM oxalic acid, which was performed in three stages (from end flowering stage to the green fruit stage) at 6 days intervals. Finally, three plants were selected from each experimental unit and leaf samples were taken and transferred to the laboratory to measure physiological traits i.e. leaf area, fresh and dry weights, total chlorophylls, chlorophyll a and chlorophyll b, total carotenoids, total sugars, total phenols and flavonoids. Also, when at least 75 percent of the fruit surface turned red, the fruits were harvested from each plot separately and immediately transferred to the Plant Physiology Laboratory of Gorgan University of Agricultural Sciences and Natural Resources. The physicochemical traits of strawberry fruits including total soluble solids (TSS), titratable acidity (TA), flavor index, vitamin C, total phenol, flavonoid, antioxidant activity, total anthocyanins were measured. Analysis of data were performed using SAS 9.2 statistical software and comparison of mean data were undertaken based on LSD statistical test.
 
Results and discussion
The results showed that the foliar application of ascorbic acid and organic acid had a significant effect on the leaf area, fresh and dry weights, total chlorophylls, chlorophyll a, chlorophyll b, carotenoids, total sugars, phenols and flavonoids. The highest mean leaf area (314.08 cm2), leaf fresh and dry weights (1.78 and 0.56 grams, respectively), chlorophyll a (0.43 mg/g), total carotenoids (0.29 mg/g), total sugars (1.43 µg/g), total phenols (0.70 mg/g) and flavonoids (0.19 mg/g) were observed in the application of oxalic acid. The control had the lowest mean in all studied traits (except carotenoids). The application of both ascorbic acid and oxalic acid resulted in an increase in photosynthetic pigments, elevating the total chlorophyll content by an average of 22% compared to the control group. Additionally, foliar spraying with ascorbic and oxalic acid led to higher levels of total soluble solids, increased antioxidant activity, and enhanced total flavonoid content in the fruit compared to the control group.Moreover, fruits harvested from plants treated with oxalic acid before harvest exhibited higher levels of total anthocyanin and phenol compared to fruits from plants treated with ascorbic acid before harvest and the control group. Fruits of plants treated with ascorbic acid compared to plants treated with oxalic acid had lower vitamin C and titratable acidity and higher flavor index. The physiological influence of oxalic acid was more superior than that of ascorbic acid and had higher positive effects in the studied traits were recorded. However, since ascorbate is one of the precursors of oxalic acid biosynthesis. Therefore, the changes caused by oxalic acid are indirectly influenced by ascorbic acid. Ascorbic acid acts as a cofactor in photosynthetic reactions and prevents the destruction of chlorophylls and carotenoids due to its antioxidant property, and by interfering in cell division and increasing the surface area of leaves; it increases photosynthesis and the production of carbohydrates. In general, according to the results, it was found that the application of ascorbic acid and oxalic acid improved the quality characteristics and the content of health related compounds of Camarosa strawberry fruit by increasing photosynthetic pigments and biomass.

Keywords

Main Subjects

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

  1. Abd El-Aziz, N.G., Mazher Azz, A.M., & El-Habba, E. (2006). Effect of foliar spraying ascorbic acid on growth and chemical constituents of Khaya senegalensis growth under salt condition. American- Eurasian Journal of Agricultural and Environmental Sciences, 1(3), 207-214.
  2. Abdel-Salam, M. (2016). Effect of foliar application with humic acid and two antioxidants on ruby seedless grapevine. Middle East Journal of Agriculture Research, 5(2), 123-131.
  3. Aghaeifard, F., Babalar, M., & Ahmadi, A. (2015). The effect of salicylic acid foliar spraying on qualitative characters of strawberry fruit (Fragaria x ananassa Camarosa). Iranian Journal of Horticultural Science, 45(4), 325-334. (In Persian with English abstract). https://doi.org/10.22059/ijhs.2015.53482
  4. Ahmed, M., Ullah, S., Razzaq, K., Rajwana, I.A., Akhtar, G., Naz, A., Amin, M., Khalid, M.S., & Khalid, S. (2021). Pre-harvest oxalic acid application improves fruit size at harvest, Physico chemical and sensory attributes of ‘Red Flesh’ apricot during fruit ripening. Journal of Horticultural Science and Technology, 4(2), 48-55.
  5. Anwar, R., Gull, S., Nafees, M., Amin, M., Hussain, Z., Khan, A.S, & Malik, A.U. (2018). Pre-harvest foliar application of oxalic acid improves strawberry plant growth and fruit quality. Journal of Horticultural Science and Technology, 1(1), 35-41.
  6. Asghari, M., Masoumi Zavariyan, A., & Yousefi Rad, M. (2016). The effect of foliar application of ascorbic acid on yield components and physiologic character of sweet corn under different irrigation regimes. Cereal Research, 6(2), 229-240. https://doi.org/20.1001.1.22520163.1395.6.2.8.8
  7. Barnes, J.D., Balaguer, L., Manrique, E., Elvira, S., & Davison, A.W. (1992). A reappraisal of the use of DMSO for the extraction and determination of chlorophyll `a` and `b` in lichens and higher plants. Environmental and Experimental Botany, 32(2), 85-90.
  8. Boudet, A.M. (2007). Evolution and current status of research in phenolic compounds. Phytochemistry, 68, 2722-2735. https://doi.org/10.1016/j.phytochem.2007.06.012
  9. Chang, C., Yang, M., Wen, H., & Chern, J. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food Drug Anal, 10, 178-182.
  10. Damatta, F.M., & Cochicho Ramalho, J.D. (2006). Impacts of drought and temperature stress on coffee physiology and production: A Review. Brazilian Journal of Plant Physiology, 18(1), 55-81. https://doi.org/1590/S1677-04202006000100006
  11. Dolatabadian, A., Modarres Sanavy, S.A.M., & Chashmi, N.A. (2008). The effects of foliar application of ascorbic acid (Vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of Canola (Brassica napus) under conditions of salt stress. Journal of Agronomy and Crop Science, 194(3), 206-213. https://doi.org/10.1111/j.1439-037X.2008.00301.x
  12. Dolatabadian, A., Modarres Sanavy, S.A.M., & Sharifi, M. (2009). Effect of water deficit stress and foliar application of ascorbic acid on antioxidants enzymes activity andsome biochemical changes in leaves of grain corn (Zea mays). Iranian Journal of Biology, 22(3), 407-422. (In Persian)
  13. El-Lethy,R., Ayad, H.S., & Reda, F. (2011). Effect of riboflavin, ascorbic acid and dry yeast on vegetative growth, essential oil pattern and antioxidant activity of geranium (Pelargonium graveolens L.). American Eurasian Journal of Agricultural and Environmental Sciences, 10, 633-638.
  14. Ergin, S., Aydogan, C., Ozturk, N., & Turhan, E. (2014). Effects of ascorbic acid application in strawberry plants during heat stress. Turkish Journal of Agricultural and Natural Sciences, 1(2), 1486-1491.
  15. (2021). Production/Yield quantities of Strawberries in World.
  16. Farokhzad, A., & Asghari, M. (2016). Effect of foliar spray with ascorbic acid on some qualitative characteristics and improving color of Apple fruit (Malus domestica Red Spur). Plant Productions, 39(3), 113-125.(In Persian with English abstract). https://doi.org/10.22055/ppd.2016.12062
  17. Fayed, T.A. (2010). Effect of some antioxidants on growth, yield and bunch characteristics of Thompson seedless grapevine. American-Eurasian Journal of Agricultural and Environmental Science, 8(3), 322-328.
  18. García-Pastor, M.E., Giménez, M.J., Valverde, J.M., Guillén, F., Castillo, S., Martínez-Romero, D., Serrano, M., Valero, D., & Zapata, P.J. (2020). Preharvest application of oxalic acid improved pomegranate fruit yield, quality, and bioactive compounds at harvest in a concentration-dependent manner. Agronomy, 10(10), 1522.https://doi.org/10.3390/agronomy10101522
  19. Ghorbani, A., Bakhshi, D., Haj Najari, H., Ghasemnezhad, M., & Taghidost, P. (2010). Phenolic compounds and antioxidant activity of some Iranian and import variant of apple in Karaj. Journal of Horticultural Science, 24(1), 83-90. https://doi.org/22067/jhorts4.v1389i1.3657
  20. Hancock,F. (2020). Strawberries, CAB International, Second edition, Boston, USA.
  21. He, Q., & Luo, Y. (2007). Enzymatic browning and its control in fresh-cut produce. Stewart Postharvest Review, 3, 123-132. https://doi.org/10.2212/spr.2007.6:3
  22. Jin, P., Wang, Y.S., Wang, C.Y., & Zheng, Y. (2011). Effect of cultural system and storage temperatureon antioxidant capacity and phenolic compounds in Food Chemistry, 124, 262-270. https://doi.org/10.1016/j.foodchem.2010.06.029
  23. Khosroabadi, H., Varasteh, F., & Seifi, E. (2016). Interaction effects of oxalic acid and storage time on some qualitative characteristics of plum fruit cv. Santa Rosa. Plant Productions, 40(2), 13-24. (In Persian with English abstract). https://doi.org/10.22055/ppd.2017.13113
  24. Kumar, N., Arora, N.K., Kaur, G., Gill, M.I.S., & Brar, J.S. (2017). Effect of pre-Harvest sprays of ascorbic Acid, calcium chloride and ethephon on fruit quality of grapes (Vitis vinifera). Journal of Krishi Vigyan, 6(1), 71-77. https://doi.org/10.5958/2349-4433.2017.00054.X
  25. Maratab, A., Liu, M., Wang, Z., Li, S., Jiang T., & Zheng, X. (2019). Pre-harvest spraying of oxalic acid improves postharvest quality associated with increase in ascorbic acid and regulation of ethanol fermentation in kiwifruit cv. Bruno during storage. Journal of Integrative Agriculture, 18(11), 2514–2520.
  26. Martinez-Espla, A., Zapata, P.J., Valero, D., Garcia-Viguera, C., Castillo, S., & Serrano, M. (2014). Preharvest application of oxalic acid increased fruit size, bioactive compounds, and antioxidant capacity in sweet cherry cultivars (Prunus avium). Journal of Agriculture and Food Chemistry, 62, 3432-3437. https://doi.org/10.1021/jf500224g
  27. Martinez-Espla, A., Serrano, M., Martínez-Romero, D., Valero, D., & Zapata, P.J. )2019(. Oxalic acid preharvest treatment increases antioxidant systems and improves plum quality at harvest and during postharvest storage. Journal of the Science of Food and Agriculture, 99(1), 235-243. https://doi.org/10.1002/jsfa.9165
  28. Mirshekari, M., Einali, A.R., & Valizadeh, J. (2017). Physiological and biochemical responses of Hibiscus sabdariffa to drought stress in the presence of salicylic acid. Iranian Journal of Plant Biology, 9(32), 21-38. (In Persian with English abstract)
  29. Muller-Moule, P., Conklin, P.L., & Niyogi, K.K. (2002). Ascorbate deficiency can limit Violaxanthin De-Epoxidase activity in vivo. Plant Physiology, 128(3), 970-977. https://doi.org/10.1104/pp.010924
  30. Munne-Bosch, S., Jubany-Mari, T., & Alegre, L. (2001). Drought-induced senescence is characterized by a loss of antioxidant defenses in chloroplasts. Plant, Cell and Environment, 24(12), 1319-1327. https://doi.org/10.1046/j.1365-3040.2001.00794.x
  31. Oz, A.T., Kafkas, E., & Bozdgan, A. (2016). Combined effects of oxalic acid treatment and modified atmosphere packaging on postharvest quality of loquats during storage. Turkish Journal of Agriculture and Forestry, 40, 433-440.
  32. Pavet, V.E., Olmos, G., Kiddle, S., Mowla, S., Kumar, J., Antoniw, M., Alvarez, E., & Foyer, C.H. (2005). Ascorbic acid deficiency activities cell death and disease resistance responses in Arabidopsis. Plant Physiology, 139, 1291–1303.
  33. Proietti, S., Moscatello, S., Leccese, A., Colla, G., & Battistelli, A. (2004). The effect of growing spinach (Spinacia oleracea) at two light intensities on the amounts of oxalate, ascorbate, and nitrate in their leaves. Journal of Horticultural Science & Biotechnology, 79(4), 606-609. https://doi.org/10.1080/14620316.2004.11511814
  34. Razavi, F., & Hajilou, J. (2016). Enhancement of postharvest nutritional quality and antioxidant capacity of peach fruits by preharvest oxalic acid treatment. Scientia Horticulture, 200, 95-101. https://doi.org/10.1016/j.scienta.2016.01.011
  35. Salami, P., Ahmadi, H., Keyhani, A., & Sarsaifee, M. (2010). Strawberry post-harvest energy losses in Iran. Researcher, 2(4).
  36. Serna-Escolano, V., Giménez, M.J., Castillo, S., Valverde, J.M., Martínez-Romero, D., Guillén, F., Serrano, M., Valero, D., & Zapata, P.J. (2021). Preharvest treatment with oxalic acid improves postharvest storage of lemon fruit by stimulation of the antioxidant system and phenolic content. Antioxidants, 10(6), 963.https://doi.org/10.3390/antiox10060963
  37. Sevinc Uzumcu, S., Koyuncu, M.A., Onursal, C.E., Guneyli, A., & Erbas, D. (2020). Effect of pre-harvest oxalic acid treatment on shelf-life of apricot cv. ‘Roxana’. Nevşehir Bilim ve Teknoloji Dergisi, 9(1), 73-80.
  38. Shazly, S.M., Eisa, A.M., Moatamed, A.M.H., & Kotb, H.R.M. (2013). Effect of some Agro-chemical pre-harvest foliar application on yield and quality of ʻSwellingʼ peach trees. Alexander Journal of Agricultural Research, 58(3), 219-229.
  39. Sheteawi, S.A. (2007). Improving growth and yield of salt-stressed soybean by exogenous application of jasmonic acid and ascorbic. International Journal of Agriculture and Biology, 9(3), 473-478.
  40. Shiukhy, S. (2022). The Effect of colored polyethylene mulch on quality characteristics, yield and chlorophyll fluorescence parameters of strawberry Cv. Camarosa. Journal of Horticultural Science, 36(2), 459-470. (In Persian with English abstract). https://doi.org/22067/jhs.2021.70983.1064
  41. Singh, J., & Mirza, A. (2018). Influence of ascorbic acid application on quality and storage life of fruits. International Journal of Current Microbiology and Applied Sciences, 7(07), 4319-4328. https://doi.org/10.20546/ijcmas.2018.707.503
  42. Slinkard, K., & Singleton, V.L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55.
  43. Smirnoff, N. (2000). Ascorbic acid, metabolism, and function of a multi-facetted Current Opinion in Plant Biology, 3(3), 229-235.
  44. Tagi, A.K., Mazid, M., & Firoz, M. (2011). A review of ascorbic acid potentialities against oxidative stress induced in plants. Journal of Agrobiology, 28(2), 97-111.
  45. Tassi, E., Pouget, J., Petruzzelli, G., & Barbafieri, M. (2008). The effects of exogenous plant growth regulators in the phytoextraction of heavy metals. Chemosphere, 71(1), 66-73. https://doi.org/10.1016/j.chemosphere.2007.10.027
  46. Varasteh, F., Arzani, K., Barzegar, M., & Zamani, Z. (2017). Pomegranate (Punica granatum) fruit storability improvement using pre-storage chitosan coating technique. Journal of Agriculture Science and Technology, 19, 389-400. https://doi.org/20.1001.1.16807073.2017.19.2.10.7
  47. Wagner, G.J. (1979). Content and vacuole/ extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology, 64, 88-93.
  48. Wang, Q., Lai, T.,Qin, G., & Tian, S. (2009). Response of jujube fruits to exogenous oxalic acid treatment based on the proteomic Plant, Cell and Physiol, 50(2), 230−242. https://doi.org/10.1093/pcp/pcn191
  49. Wu, F., Zang, D., Zang, H., Jiang, G., Su, X., Qu, H., Jang, Y., & Duan, X. (2011). Physiological and biochemical response of harvested plum fruit to oxalic acid during ripening or shelf-life. Food Research International, 44, 1299-1305.
  50. Zheng, X., & Tian, Sh. (2006). Effect of oxalic acid on control of postharvest browning of litchi fruit. Food Chemistry, 96, 519-523.
  51. Zhu, Y., Yu, J., Brecht, J., Jiang, T., & Zheng, X.(2016). Pre-harvest application of oxalic acid increases quality and resistance to Penicillium expansum in kiwifruit during postharvest storage. Food Chemistry, 1(190), 537-543. https://doi.org/10.1016/j.foodchem.2015.06.001
  52. Zulaikha, R. (2013). Effect of foliar spray of ascorbic acid, Zn, seaweed extracts force and biofertilizers on vegetative growth and root growth of olive (Olea europea) transplants cv. Hogblanca. International Journal of Pure and Applied Sciences, 17(2), 79-89.

 

 

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