Document Type : Research Article
Authors
1 Ferdowsi University of Mashhad
2 Urmia University
Abstract
Introduction: Salinity is one of the most important environmental factors that regulates plant growth and development, and limits plant production. Researchers have shown that some plant growth regulators such as nitric oxide improve the plants resistance to environmental stresses such as heat, cold, drought and salinity. Sodium nitroprusside (SNP) commonly has been used as nitric oxide (NO) donor in plants. NO is a diffusible gaseous free radical. Low concentrations of NO inhibit the production of reactive oxygen species and protect plants against ROS damages. The aim of this study was to evaluate the role of SNP as NO donor on salt tolerance of Calendula officinalis and its effects on some morphological, physiological and biochemical characteristics of this plant.
Materials and Methods: In this study, the effects of salinity (0, 25, 50, 75 and 100 mM) and sodium nitroprusside (0.0, 0.25, 0.50 and 0.75 mM) on morphological and physiological characteristics of Calendula officinalis L. were investigated. Total leaf area and number of leaves were determined in the end of the experiment. Electrolyte leakage was used to asses’ membrane permeability. This procedure was based on Lutts et al.,1995. Soluble sugars were extracted and estimated by the method of Irigoyen et al., 1992. Chlorophyll a, b and carotenoid content were calculated from the absorbance of extract at 653, 666 and 470 nm using the formula of Dere et al., 1998. Proline was extracted by the method of Bates et al., 1973. DPPH radical- scavenging activity of sample was performed as described previously of Cleep et al., 2012. The SAS software was used for the analysis of variance (ANOVA), comparisons with P
Keywords
1-Agarwal S., and Pandy V. 2004. Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia Plantarum, 48: 555-560.
2-An L., Liua Y., Zhang M., Chen T., and Wang X. 2005. Effects of nitric oxide on growth of maize seedling leaves in the presence or absence of ultraviolet-B radiation. Journal of plant physiology, 162:317–326.
3-Azari A., Modares Sanavi S.A.M., Askari H., Ghanati F., Naji A.M., and Alizadeh B. 2012. Effect of salt stress on morphological and physiological traits of two species of rapeseed (Brassica napus and B. rapa). Iranian Journal of Crop Sciences, 14(2):121-135. (in Persian with English abstract)
4-Bajji M., Lutts S., and Kinet J.M. 2001. Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Science, 160: 669-681.
5-Bates L.S., Waldran R.P., and Teare I.D. 1973. Rapid determination of free proline for water studies. Plant Soil, 39: 205–208.
6-Beligni M.V., Lamattina L. 2000. Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light inducible responses in plants. Planta, 210:215–221.
7-Brayant J.P., Chapin F.S., and Klein D.R. 1983. Carbon nutrient balance of boreal plants in relation to vertebrate herbivory. Oikos, 40: 357-368.
8-Bohnert H.J., and Jensen R.G. 1996. Strategies for engineering water stress tolerance in plants. Trends Biotechnol, 14: 89–97.
9-Celep E., Aydin A., Kirmizibekmez H., and Yesilada E. 2013. Appraisal of in vitro and in vivo antioxidant activity potential of cornelian cherry leaves. Food and Chemical Toxicology, 62: 448-455.
10-Chaparzadeh N., Amico M. D’., Khavari-Nejad R., Izzo R., and Navari-Izzo F. 2004. Antioxidative responses of Calendula offıcinalis under salinity conditions. Plant Physiology and Biochemistry, 42: 695–701.
11-Chinnusamy V., Jagendorf A.,and Zhu J.K. 2005. Understanding and improving salt tolerance in plants. Crop Sciences, 45: 437-448.
12-Coue´e I., Sulmon C., Gouesbet G., and Amrani A. 2006. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. Journal of Experimental Botany, 57:449–459.
13-Corpas F.J., Barroso J.B., Carreras A., Quiros M., Leo´n A.M., Romero- Puertas M.C., Esteban F.J., Valderrama R., Palma J.M., and Sandalio LM. 2004. Cellular and subcellular localization of endogenous nitric oxide in young and senescent pea plants. Plant Physiology, 136:2722–2733.
14-Dere S., Gines T. and Sivaci R. 1998. Spectrophotometric determination of chlorophyll- a, b and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22: 13-17.
15-Fan H., Guo S., Jiao Y., and Zhang R, Li. 2007. Effects of exogenous nitric oxide on growth, active oxygen species metabolism, and photosynthetic characteristics in cucumber seedlings under NaCl stress. Frontiers of Agriculture in China, 1:308–314.
16-FAO. 2011. FAO land and plant nutrition management service. Available at: http://www.fao.org/ag/agl/agll/spush/. Accessed 25 November 2011.
17-Geholt H. S., Purohit A., and Shekhawat N.S. 2005 Metabolic changes and protein patterns associated with adaptation to salinity in Sesamun indicum cultivars. Journal of Cell and Molcular Biology, 4: 31-39.
18-Hayat S., Yadav S., Wani A., Irfan M., and Ahmad A. 2011. Nitric Oxide Effects on Photosynthetic Rate, Growth, and Antioxidant Activity in Tomato, International Journal of Vegetable Science, 17: 333-348.
19-Iraki N.M., Bressan R.A., Hasegawa P.M. and Carpita N.C. 1989. Alteration of the Physical and Chemical Structure of the Primary Cell Wall of Growth-Limited Plant Cells Adapted to Osmotic Stress. Plant Physiology, 91: 39-47.
20-Irigoyen J.J., Emerich D.W., and Sa´nchez-D´ıaz M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84: 55–60.
21-Kang H. M. and Saltveit M.E. 2002. Effect of chilling on antioxidant enzymes and DPPH-radical scavenging activity of high- and low-vigour cucumber seedling radicles. Plant Cell Environment, 25: 1233-1238.
22-Kim J.H. and Lee C.H. 2005. In vivo deleterious effects specific to reactive oxygen species on photosystem II afterphotooxidative treatment of rice leaves. Plant Sciences, 168: 1115-1125.
23-Laspina N.V., Groppa M.D., Tomaro M L., and Benavides M.P. 2005. Nitric oxide protects sunflower leaves against Cd-induced oxidative stress. Plant Sciences, 169: 323-330.
24-Leshem Y.Y., and Hamaraty E. 1996. Plant aging: the emission of NO and ethylene and effect of NO-releasing compounds on growth of pea (Pisum sativum L.) foliage. Journal of Plant Physiology, 148, 258–263.
25-Li Q., Niud H., Yind J., Wanga M., Shaob H., Dengd D., Chend X., Rend J., and Li Y. 2008. Protective role of exogenous nitric oxide against oxidative-stress induced by salt stress in barley (Hordeum vulgare) . Colloids and Surfaces B: Biointerfaces, 65: 220–225.
26-Luttes S., Kinet J.M., and Bouharmont J. 1995. Changes in Plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany, 46,1843-1852.
27-Misra N., and Saxena P. 2009. Effect of salicylic acid on proline metabolism in lentil grown nder salinity stress. Plant Science, 177: 181-188.
28-Molassiotis A., Sotiropoulos T., Tanou G., Diamantidis G., and Therios I. 2006. Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM9 (Malus domestica Borkh). Environmental and Experimental Botany, 56: 54–62.
29-Martinez G.R., Mascio P.D., Bonini M.G., Augusto O., Briviba K., and Sies H. 2000. Peroxynitrite does not decompose to singlet oxygen (1gO2) and nitroxyl (NO-). Proceedings of the National Academy of Sciences, 97:10307–10312.
30-Nasibi F, and Kalantari K.M. 2009. Influence of nitric oxide in protection of tomato seedling against oxidative stress induced by osmotic stress. Acta Physiologia Plantarum, 31:1037–1044.
31-Neill S., Desikan R., Clarke A., and Hancock J.T. 2002 Nitric oxide is a novel component of abscisic acid signalling in stomatal guard cells. Plant Physiology, 128:13–16.
32-Pessarakli M. 1999: Handbook of Plant and Crop Stress. Second Edition. Marcel Dekker, Inc. New York. 545p.
33-Prabijot K.G., Arun D.S., Prabhjeet S., and Singh B. 2001. Effect of various abiotic steress on the grown in light and darkness. Bulg. Journal of Plant Physiology, 27: 72-84.
34-Qiao W., Xiao S., Yu L., and Fan L.M. 2009. Expression of a rice gene OsNOA1 re-establishes nitric oxide synthesis and stress-related gene expression for salt tolerance in Arabidopsis nitric oxideassociated 1 mutant Atnoa1. Environmental and Experimental Botany, 65:90–98.
35-Radin J.W., and Ackerson, R.C. 1981. Water relations of cotton plants under nitrogen deficiency .II. Stomatal conductance, photosynthesis and abscisic acid. Plant Physiology, 67,115-119.
36-Rawson H.M., long M.j., and Munnus R. 1988. Growth and development in NaCl treated plant. Journal of Plant Physiology, 15:519-527.
37-Ribeiro E.A., Cunha F.Q., Tamashiro W.M.S.C., and Martins I.S. 1999. Growth phase-dependent subcellular localization of nitric oxide synthase in maize cells. FEBS Letters, 445:283–286.
38-Rosa-Ibara M.D.L., and Maiti R.K. 1995. Biochemical mechanism in glossay sorgum lines for resistance to salinity stress. Plant Physiology, 146:515-519.
39-Shi S.Y., Wang G., Wang Y.D., Zhang L.G., and Zhang L.X. 2005. Protective effect of nitric oxide against oxidative stress under ultraviolet-B radiation. Nitric Oxide, 13:1–9.
40-Singh A.K., and Dubey R.S. 1995. Changes in chlorophyll a and b contents and activities of photosystems 1 and 2 in rice seedlings induced by NaCl. Photosythetica, 31: 489–499.
41-Singh H.P., Batish D.R., Kaur G., Arora K., and Kohli R.K. 2008 Nitric oxide (as sodium nitroprusside) supplementation ameliorates Cd toxicity in hydroponically grown wheat roots. Environmantal and Experimental Botany, 63:158–167
42-Song L.L., Ding W., Zhao M.G., Sun B.T., and Zhang L.X. 2006. Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed. Plant Science, 171:449–458.
43-Stevens J., Senaratna T., and Sivasithamparam K. 2006. Salicylic acid induces salinity tolerance in tomato (Lycopersicon esculentum cv. Roma): associated changes in gas exchange, water relations and membrane stabilisation. Plant Growth Regulation, 49: 77–83.
44-Terasaki S., Sakurai N., Yamamoto R., Wada N., and Nevins D.J. 2001. Changes in cell wall polysaccharides of kiwifruit and the viscoelastic properties detected by laser Doppler method .Journal of the Japanese Society for Horticultural Science, 70:572–580.
45-Wang H.H., Liang X.L., Wan Q., Wang X.M., and Bi Y.R. 2009. Ethylene and nitric oxide are involved in maintaining ion homeostasis in Arabidopsis callus under salt stress. Planta, 230:293–307.
46-Wu X., Zhu W., Zhang H., Ding H., and Zhang H . J. 2011. Exogenous nitric oxide protects against salt-indaced oxidative stress in the leaves from two genotypes of tomato ( Lycopersicum esculentum Mill.). Acta Physiologiae Plantarum, 33:1199-1209.
47-Zhang M., An L., Feng H., Chen T., Chen K., Liu Y., Tang H., Chang J., and Wang X. 2003. The cascade mechanisms of nitric oxide as a second messenger of ultraviolet-B in inhibiting mesocotyl elongations. Photochemistry and Photobiology, 77:219–225.
48-Zhang Y.Y., Wang L.L., Liu Y.L., Zhang Q., Wei Q.P.,and Zhang W.H. 2006. Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na?/H? antiport in the tonoplast. Planta, 224:545–555.
49-Zhang Y., Han X., Chen X., Jin H., and Cui X. 2009. Exogenous nitric oxide on antioxidative system and ATPase activities from tomato seedlings under copper stress. Scientia Horticulturae, 123:217–223.
50-Zheng C.F., Dong J.G., Liu F.L., Dai T.B., Liu W.C., Jing Q., and Cao W.X. 2009. Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity. Environmental and Experimental Botany, 67:222–227.
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