تأثیر مرحله بلوغ میوه بر ویژگی‌های فیزیکیوشیمیایی و پلی‌فنل‌های میوه اترج صغیر (Citrus medica var. medica Proper.)

نوع مقاله : مقالات پژوهشی

نویسندگان

• نحله تقوایی فرد ¹
• عسکر غنی ²
• سید مهدی حسینی فرهی ³

¹ دانشگاه آزاد اسلامی واحد یاسوج

² دانشگاه جهرم

³ گروه علوم باغبانی، دانشگاه آزاد اسلامی، واحد یاسوج، ایران و گروه پژوهشی کشاورزی پایدار و امنیت غذایی، واحد یاسوج، دانشگاه آزاد اسلامی، یاسوج، ایران

https://doi.org/10.22067/jhs.2020.61947.0

چکیده

به منظور بررسی تأثیر مرحله بلوغ میوه بر ویژگی­های بیوشیمیایی بخش­های مختلف میوه اترج صغیر، آزمایشی به صورت فاکتوریل بر پایه طرح بلوک­های کامل تصادفی با دو فاکتور و چهار تکرار اجرا گردید. فاکتور اول، دارای چهار سطح شامل مراحل مختلف بلوغ میوه (سبزرسیده، تغییررنگ پوست، زردرسیده و بسیاررسیده) بود. فاکتور دوم دارای چهار سطح شامل بخش‌های مختلف میوه اترج صغیر (فلاودو، آلبیدو، تفاله و آب‌میوه) بود. مهمترین صفات اندازه­گیری شده شامل میزان فلاون و فلاونول، فلاونوئید کل، ترکیبات فنلی کل و فعالیت آنتی­اکسیدانی بود. تغییرات فیزیکی بخش­های مختلف میوه و همچنین ویژگی­های مربوط به آب­میوه (اسیدیته، مواد جامد محلول، اسیدیته قابل­تیتر و ویتامین ث) در طی بلوغ میوه اندازه­گیری شد. مهمترین ترکیبات فیتوشیمیایی بخش فلاودو شامل درصد و عملکرد اسانس، محتوی کلروفیل و پلی­فنل­ها ­(روتین، ترانس-فرولیک اسید، هسپریدین، هسپرتین و کوئرستین) در طی رسیدن میوه بررسی شد. نتایج نشان­دهنده تاثیر معنی­دار مرحله بلوغ میوه بر بیشتر صفات مورد بررسی بود. بیشترین میزان فلاون و فلاونول (377/0 میلی­گرم برگرم) و فلاونوئید کل (38/16 میلی­گرم برگرم) مربوط به فلاودو در مرحله بسیار رسیده بود. بالاترین میزان اسانس فلاودو (37/2 درصد حجمی-وزنی) در مرحله تغییر رنگ اندازه­گیری شد و با پیشرف بلوغ محتوی کلروفیل دستخوش تغییرات نزولی گردید. پیشرفت بلوغ باعث کاهش بیشتر اجزای پلی­فنل­های اندازه­گیری شده شامل هسپریدین (86/1 به 68/0 میلی­گرم برگرم)، کوئرستین (62/1 به 89/0 میلی­گرم برگرم) و روتین (42/0 به 17/0 میلی­گرم برگرم) گردید. به طور کلی بخش فلاودو به عنوان مهم­ترین بخش دارویی میوه اترج و مراحل ابتدایی بلوغ زمان مناسب­تری برای برداشت تعیین گردید.

کلیدواژه‌ها

• اترج صغیر
• اسانس
• فلاودو
• فعالیت آنتی‌اکسیدانی
• هسپریدین

20.1001.1.20084730.1400.35.3.9.3

موضوعات

• گیاهان دارویی

عنوان مقاله [English]

The Effect of Fruit Maturity on Physicochemical Properties and Polyphenols in Citron Fruit (Citrus medica var. medica Proper.)

نویسندگان [English]

• Nahle Taghvaeefard ¹
• Askar Ghani ²
• Seyyed Mehdi Hosseinifarahi ³

¹ Islamic Azad University, Yasooj Branch

² Jahrom University

³ Department of Horticultural Science, Islamic Azad University, Yasuj Branch, Yasuj, Iran and Sustainable Agriculture and Food Security Research Group, Yasuj Branch, Islamic Azad University, Yasuj, Iran

چکیده [English]

Introduction: The harvest time and type of plant part have important roles in obtaining maximum amounts of active substances from medicinal plants. Medicinal plants vary in terms of phenological stages and maturity and each medicinal plant can be evaluated separately in research. Determining the exact time of harvest and selecting a plant organ for maximum yield of secondary metabolites can be very important and economically effective. Othroj-Saghir (Citrus medica var. medica Proper) or Othroje-Kabir (Citrus medica var. macrocarpa) is one of the most important citrus species. The southern provinces of Iran, especially the southern cities of Fars province, are the largest areas in which this species is cultivated. In addition to fruit juice, other parts of the fruit especially the flavedo, can be used for medicinal purposes and in food industries. In this study, for the first time, some physical and biochemical changes of different parts of Citrus medica var medica fruits were evaluated during fruit ripening. Also, some active substances (i.e. essential oil content, flavonoid components, etc.) of the flavedo were measured as the most important medicinal parts of the fruit through different stages of ripening.
Materials and Methods: In this study, the effects of fruit ripening on the biochemical properties of different parts of Citron fruit were evaluated. A factorial experiment was set up according to a randomized complete block design (RCBD) with two factors and four replications. The first factor included four levels: different stages of fruit maturity (green mature, intermediate, yellow ripe and over ripe stage). The second factor had four levels: the different parts of the Citron fruit (i.e. outer skin or flavedo, inner skin or albedo, pulp and juice). The most important measured traits were flavon and flavonols, total flavonoids, total phenolic compounds and antioxidant activity. Physical changes in different parts of the fruit (fresh and dry weight of flavedo and albedo, fresh weight of fruit, fruit diameter and height of fruit, etc.) were measured.
Assessments also carried out to identify several features of fruit juice such as acidity, total soluble solids (TSS), titrable acidity (TA) and vitamin C during fruit ripening. Chemical measurements were aimed at determining the most important phytochemical compounds of the flavedo section during fruit ripening. These features included the essential oil percentage and yield, chlorophyll content and polyphenols constituents (rutin, trans-ferulic acid, hesperidin, hesperetin and quercetin by HPLC).
Results and Discussion: The results showed that the fruit maturity stage significantly affected most of the studied traits. The highest amounts of flavon and flavonols (0.377 mg quercetin/g dry weight) and total flavonoids (16.38 mg quercetin/g dry weight) were measured in the flavedo at the over ripe stage. The antioxidant activity initially increased during fruit ripening, but it decreased slightly (75.0%) from the yellow stage to the over ripe stage. The lowest rate of antioxidant activity (53.6%) was observed in the green stage and the highest rate (78.4%) was measured in the yellow ripe stage. The highest amounts of antioxidant activity (83.3% and 73.8%) were measured in the albedo and flavedo samples, respectively, and the lowest amount (54.1%) was recorded in the pulp section. The highest amount of flavedo essential oil (2.37%v/w) was measured in intermediate stage. The chlorophyll content with maturity progress was decreased. Progress in fruit maturity was reduced the most components of polyphenols. The maximum amount of hesperidin, as an important phenolic component, was identified in the peel of Citron fruit (1.86 mg/g dry weight) at the green mature stage. Its amount decreased through the stages of maturity, so that the lowest amount (0.68 mg/g dry weight) was measured at the over ripe stage.
Conclusion: In general, the stage of maturity and plant part had important effects on the amount of biochemical traits. The flavedo part of Citron fruit showed superior medicinal properties. Different active substances react variedly during maturity and so the preferable composition should be harvested according to the desired conditions. The best stages to reach maximum essential oil percentage and polyphenol components are the early stages of maturity (i.e. green mature and intermediate stages).

کلیدواژه‌ها [English]

• Antioxidant activity
• Citrus medica var. medica Proper
• Essential oil
• Flavedo
• Hesperidin

مراجع

• Ahankoub Ro M., Foutohi Ghazvini R., and Fattahi Moghadam J. 2014. Investigation biochemical diversity of peel and pulp from some natural citrus biotypes. Journal of Plant Production Research 21(4): 81-98. (In Persian with English abstract)
• Bhuiyan M.N.I., Begum J., Sardar P.K., and Rahman MS. 2009. Constituents of peel and leaf essential oils of Citrus medica Journal of Scientific Research 1: 387-392.
• Bhuyan N., Barua P.C., Kalita P., and Saikia A. 2015. Physico-chemical variation in peel oils of Khasi mandarin (Citrus reticulata Blanco) during ripening. Indian Journal of Plant Physiology 20: 227-231.
• Boudries H., Madani, K., Touati, N., Samiha S., Medouni S., and Chibane M. 2012. Pulp antioxidant activities, mineral contents and juice nutritional properties of Algerian clementine cultivars and mandarin. African Journal of Biotechnology 11(18): 4258-4267.
• Bourgou S., Rahali F.Z., Ourghemmi I., and Saidani Tounsi M. 2012. Changes of peel essential oil composition of four Tunisian citrus during fruit maturation. The Scientific World Journal 2012: 1-10.
• Carmona L., Zacarias L., and Rodrigo M.J. 2012. Stimulation of coloration and carotenoid biosynthesis during postharvest storage of Navelina orange fruit at 12°C. Postharvest Biology and Technology 74: 108-117.
• Del Río J.A., Fuster M.D., Gomez P., Porras I., García-Lidon A., and Ortuno A. 2004. Citrus lemon: a source of flavonoids of pharmaceutical interest. Food Chemistry 84: 457–461.
• Dere S., Gunes T., and Sivaci R. 1998. Spectrophotometric determination of chlorophyll a, b and total carotenoid contents of some algae species using different solvents. Journal of Botany 22: 13- 17.
• Dong X., Hu Y., Li Y., and Zhou Zh. 2019. The maturity degree, phenolic compounds and antioxidant activity of Eureka lemon [Citrus lemon (L.) Burm. f.]: A negative correlation between total phenolic content, antioxidant capacity and soluble solid content. Scientia Horticulturae 243: 281-289.
• Ebrahimzadeh M.A., Hosseinimehr S.J., Mahmoudi M., Ghayekhloo M.R., and Hosseini M. 2005. Measuring the amount of vitamin c in citrus fruits by a two step oxidation-reduction titration method. Journal of Mazandaran University of Medical Sciences 48: 26-32. (In Persian with English abstract)
• Fatahi Moghadam J., Seyed Ghasemi S. E., and Najafi K. 2018. Evaluation of fruit physico-chemical characteristics of new Mandarins Noushin (Citrus reticulata Noushin) and Shahin (Citrus reticulata cv. Shahin) during different harvesting times. The Plant Production (Scientific Journal of Agriculture) 40(4): 77-90. (In Persian with English abstract)
• Fatahi Moghadam J., Seyedghasemi S.E., and Madani S. 2017. The effect of five rootstocks on physical, mechanical and chemical characteristics of Yashar fruits -a new Mandarin- during ripening stages. Journal of Plant Production (Journal of Agricultural Sciences and Natural Resources) 24(2): 109-123. (In Persian with English abstract)
• Fattahi J., Hamidoghli Y., Fotouhi R., Ghaseminejad M., and Bakhshi D. 2011. Assessment of fruit quality and antioxidant activity of three citrus species during ripening. South Western Journal of Horticulture, Biology and Environment 2: 113-128.
• Fattahi J., Hamidoghli Y., Fotouhi R., Ghasemnejad M., and Bakhshi D. 2011. Evaluation of physicochemical properties and antioxidant activity of the peel of different commercial citrus species. Journal of Horticultural Science 25(2): 211-217. (In Persian with English abstract)
• Fotouhi Ghazvini R., and Fattahi Moghadam J. 2010. Citrus cultivation in Iran. Gilan: Gilan University Press.
• Ghasemi SH., Hemati K., Bashiri Sadr Z., Ghasem Nezhad A., and Ghasemi M. 2011. Quantitation of phenolic compounds in tissues of lime (Citrus aurantifolia) fruit during growth and maturation. Journal of Food Science and Technology 31(8): 69-75. (In Persian with English abstract)
• Habibi, F., and Ramezanian A. 2017. Changes in Physicochemical and Bioactive Compounds of Blood Orange Fruit ‘Sanguine’ during Ripening. Iranian Journal of Horticultural Science and Technology 18 (4): 365-376. (In Persian with English abstract)
• Hemati K.H., and Omidbaigi R. 2003. Investigation of the naringin flavonoid content during different fruit development of marsh grapefruit (Citrus paradisii). Journal of Agricultural Science 10(2): 65-72. (In Persian with English abstract)
• Hosni K., Zahed N., Chrif R., Abid I., Medfei W., Kallel M., Ben Brahim N., and Sebei H. 2010. Composition of peel essential oils from four selected Tunisian citrus species: Evidence for the genotypic influence. Food Chemistry 123: 1098-1104.
• Koca N., Karadeniz F., and Burdulu H.S. 2006. Effect of pH on chlorophyll degradation and colour loss blanched green peas. Journal Food Chemistry 100: 609- 615.
• Kumar, S., Narayan Jena S., and Nair N.K. 2010. ISSR polymorphism in Indian wild orange (Citrus indica Tanaka, Rutaceae) and related wild species in North-East India. Scientia Horticulturae 123: 350-359.
• Menichini F., Tundis R., Bonesi M., Loizzo M.R., Conforti F., Statti G., Di Cindi B., Houghton P.J., and Menichini F. 2009. The influence of fruit ripening on the phytochemical content and biological activity of Capsicum chinense Habanero. Food Chemistry 114: 553-560.
• Menchini F., and Tundis R. 2011. Phytochemichal profile, antioxidant, anti-inflammatory and hypoglycemic potential of hydroalcoholic extracts from Citrus medica cv. Diamante flowers, leaves and fruits at two maturity stages. Food and Chemical Toxicology 49: 1549-55.
• Mirheydar H. 2012. Fruits. Herbal education 2, Ninth ed, Islamic culture publishing office, Tehran, Iran.
• Mišan A., Mimica-Dukić N., Mandić A., Sakač M., Milovanović I., and Sedej I. 2011. Development of a rapid resolution HPLC method for the separation and determination of 17 phenolic compounds in crude plant extracts. Open Chemistry 9(1): 133-142.
• Oke F., Aslim B., Ozturk S., and Altundag S. 2009. Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia ten. Food Chemistry 112: 874-879.
• Pailly O., Tison G., and Amourouxb A. 2004. Harvest time and storage conditions of Star Ruby grapefruit (Citrus paradisi Macf.) for short distance summer consumption. Postharvest Biology and Technology 34: 65-73.
• Popova M., Bankova V., Butovska D., Petkov V., Nikolova-Damyanova B., Sabatini A.G., Marcazzan G.L., and Bogdanov S. 2004. Validated methods for the quantification of biologically active constituents of poplar-type propolis. Phytochemistry Analysis 15: 235-240.
• Rekha C., Poornima G., Manasa M., Abhipsa V., Pavithra Devi J., Vijay Kumar H.T., and Prashith Kekuda T.R. 2012. Ascorbic acid, total phenol content and antioxidant activity of fresh juices of four ripe and unripe citrus fruits. Chemical Science Transactions 1: 303-310.
• Serrano M., Martinez-Romero D., Guillen F., Castillo S., and Valero D. 2006. Maintenance of broccoli quality and functional properties during cold storage as effected by modified atmosphere packaging. Postharvest Biology and Technology 39: 61-68.
• Seyedghasemi S.E., Fattahi Moghadam J., and Babakhani B. 2018. Investigation of bioactive compounds changes of two Lisbon (Citrus limon Lisbon) and Cook eureka lemon (C. limon cv. Cook Eureka) varieties during ripening. Journal of Plant Research (Iranian Journal of Biology) 31(1): 182-193. (In Persian with English abstract)
• Sharma K., Mahato N., and Lee Y.R. 2018. Extraction, characterization and biological activity of citrus flavonoids. Reviews in Chemical Engineering 35(2): 1-20.
• Tripoli E., Guardia M.L., Giammanco S., Majo D.D., and Giammanco M. 2007. Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A Review. Food Chemistry 104: 466-479.
• Venturini N., Barboni T., Curk F., Costa J., and Paolini J. 2014. Volatile and flavonoid composition of the peel of Citrus medica var. Corsican fruit for quality assessment of its liqueur. Food Technology and Biotecnology 52: 403-410.
• Wang H.C., Huang X.M., Hu G.B., Yang Z.Y., and Huang H.B. 2005. A comparative study of chlorophyll loss and its related mechanism during fruit maturation in the pericarp of fast and slow-degreening Litchi pericarp. Scientia Horticulturae 106: 247- 257.
• Wang Y.C., Chuang Y.C., and Ku Y.H. 2007. Quantitation of bioactive compounds in citrus fruits cultivated in Taiwan. Food Chemistry 102: 1163-1171.
• Wojdylo A., Oszmianski J., and Czemerys R. 2007. Antioxidant activity and phenolic compound in 32 selected herbs. Food Chemistry 1005: 940-949.
• Wu Z., Li H., Yang Y., Zhan Y., and Dawei T. 2013. Variation in the components and antioxidant activity of Citrus medica var. sarcodactylis essential oils at different stages of maturity. Industrial Crops and Products 46: 311-316.
• Xu G., Ye X., Liu D., Ma Y., and Chen J. 2008. Composition and distribution of phenolic acids in Ponkan (Citrus poonensis ex Tanaka) and Huyou (Citrus paradisi Macf. Changshanhuyou) during maturity. Journal of Food Composition and Analysis 21: 382-389.
• Yoo K.M., Lee K.W., Park J.B., Lee H.J., and Hwang I.K. 2004. Vareation in major antioxidants and total antioxidant activity of Yuzu (Citrus junos ex Tanala) during maturation and between cultivars. Agricultural and Food Chemistry 52: 5907-5913.
• Yu J., Deepak V., Dandekar A., Romeo T., Toledo B., Rakesh K., Singh B., Bhimanagouda K., and Patil S. 2007. Supercritical fluid extraction of limonoids and naringin from grapefruit (Citrus paradisi ) seeds. Food Chemistry 105: 1026-1031.