نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه زنجان

2 پژوهشکده بیوتکنوژی کشاورزی

چکیده

به منظور بررسی اثرات شوری بر طول شاخه، تعداد برگ، تعداد جوانه جدید، کلروز، نکروز و غلظت عناصر سدیم، کلر، پتاسیم، نیتروژن و فسفر بر ریزنمونه های تهیه شده از کشت نوک شاخساره گلابی رقم درگزی ( Pyrus Communis cv. Dargazi) آزمایشی شامل تیمارهای سطوح مختلف شوری؛ صفر (شاهد)، 40، 80، 120، 160 میلی مولار کلرید سدیم در قالب طرح کاملاً تصادفی با چهار تکرار در سال 1391 در دانشگاه زنجان انجام شد. بعد از 6 هفته دوره کشت درون شیشه ای تحت تنش شوری، صفات ذکر شده مورد ارزیابی قرار گرفتند. طول شاخه و تعداد برگ با افزایش سطح شوری کاهش و تعداد برگ کلروزه و نکروزه تحت تأثیر شوری بطور معنی داری افزایش یافتند. بعلاوه با افزایش سطح شوری غلظت نیتروژن و پتاسیم بافت گیاه کاهش و میزان سدیم و کلر بافت افزایش یافت. غلظت فسفر بافت گیاهی تحت تأثیر تنش شوری قرار نگرفت.

کلیدواژه‌ها

عنوان مقاله [English]

Physiological Response of Pear (Pyrus Communis cv.Dargazi) to Salinity Stress Under In vitro Conditions

نویسندگان [English]

  • Fateme Zafari 1
  • Mohammad Esmaeil Amiri 1
  • Ali Vatanpour Azghandi 2

1 University of Zanjan

2 Agricultural Biotechnology Research Institute

چکیده [English]

This study was conducted to find out the influence of in vitro salinity on growth parameters shoot length, number of leaves, number of new buds, the chlorophyll, chlorosis and necrosis and absorption of sodium, chloride, potassium, nitrogen and phosphorus of pear (Pyrus communis cv. Dargazi) in vitro propagated shoots. The experiment was conducted as a complete randomized design with 5 salinity levels; (control), 40, 80, 120 and 160 mM of sodium chloride in 2013 at Zanjan University, Zanjan, Iran. Different mentioned parameters were assessed after 6 weeks of culture. Shoot length and leaf number per explant decreased and number of chlorotic and necrotic leaves increased with increasing salinity. Increasing salinity levels also decreased nitrogen and potassium content of plant tissues while their sodium and chloride contents increased. Phosphorus was not affected by salinity.

کلیدواژه‌ها [English]

  • Pear
  • salinity
  • growth
  • Nutrient uptake
  • In vitro conditions
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