Investigating the Effect of Salicylic Acid on Reduce Salinity Stress in Tomatoes

Document Type : Research Article

Authors

• Jaber Panahandeh
• Mohammad Sedigh Zare far
• Alireza Motallebi-Azar
• Fariborz Zare Nahandi
• Mina Amani

Department of Horticultural Science and Engineering, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

https://doi.org/10.22067/jhs.2023.79421.1206

Abstract

Introduction
Various methods of stress directly affected the growth and production yield of numerous plants. For example, environmental stress reduces the tomato manufacturing by the disrupting its natural metabolism, or, salinity stresses affect the it is growth and development from the germination stage to the fruit ripening stage. Salinity in tomatoes by stimulating the biosynthesis of growth regulators such as ethylene and abscisic acid leads to the acceleration of the aging of the leaves. Therefore, development of different methods to induce salinity stress tolerance in plants is necessary. Some approaches were studied to develop the salinity tolerant plants such as genetic breeding, environmental improvements and usage of phytohormones and signal molecules. Salicylic acid or orthohydroxybenzoic acid plays an important role in regulating the physiological and biochemical responses of plants to stress conditions, which improves the plant's resistance to adverse environmental conditions. For instance, salicylic acid is a facile and effective way to increase plant productivity under salt stress conditions. Considering the positive effects of salicylic acid in modulating the effects of salinity, this study was conducted with the aim of investigating the effects of salicylic acid’s usage in modulating the harmful effects of salinity on some vegetative, physiological, quantitative and qualitative characteristics of two tomato cultivars of Baneh local mass and Semi Dwarf line.
Materials and Methods
To investigate the effect of salicylic acid in modulating the effects of salinity stress in tomato, a factorial experiment was conducted in the form of a randomized complete block design, with 12 treatments, in 3 replications and with a total of 36 experimental units in the hydroponic greenhouse of the Department of Horticulture, Faculty of Agriculture, and university of Tabriz. The treatments included two levels of salicylic acid (0 and 1 mM) and salinity levels (0, 35 and 70 mM NaCl) on two tomato cultivars of Baneh and Semi Dwarf.
Results and Discussion
The results showed that in Baneh and Semi Dwarf cultivars, the increase in salinity levels caused a decrease in vegetative indices, meanwhile the treatment of salicylic acid along with salt stress increased same indices. Also, salt stress caused yield reduction in both Baneh and Semi Dwarf cultivars. By examining the qualitative indicators, it was observed that titratable acidity and vitamin C increased with salt stress and salicylic acid treatment in both cultivars. In terms of physiological indicators, the amount of proline increased at different salinity levels with salicylic acid treatment, but the amount of leaf chlorophyll index decreased with the increase of same condition.
Conclusion
The results of testing the effect of salicylic acid and the effects of salinity stress on vegetative, quantitative, qualitative and physiological indicators in Baneh and Semi Dwarf tomatoes showed a remarkable difference in terms of significance. In terms of vegetative traits; Plant height, leaf area index, shoot wet in Baneh and Semi Dwarf cultivars decreased with increasing salinity levels of vegetative indices, but salicylic acid treatment along with salinity stress increased same indices. Indicators such as yield, fresh weight of fruit, and percentage of dry matter of fruit showed different responses to different levels of salinity and salicylic acid treatment. The fresh weight of fruit increased with the application of salicylic acid. Also, salt stress caused an increase in the percentage of dry matter of the fruit. But salt stress caused yield reduction in both Baneh and Semi Dwarf cultivars. In terms of quality indicators; the amount of titratable acidity and vitamin C increased with salt stress and salicylic acid treatment in both cultivars. In terms of physiological indicators, the level of proline increased across various salinity levels with salicylic acid treatment. However, the leaf chlorophyll index decreased with rising salinity levels, even in the presence of salicylic acid treatment. Overall, salinity stress caused a decrease in most analyzed traits in the Baneh and Semi Dwarf cultivars. Nevertheless, it led to improvements in certain quality traits. Additionally, salicylic acid treatment enhanced the mentioned indices in most of the examined traits in both cultivars. Therefore, considering the positive effects of salicylic acid treatment on Baneh and Semi Dwarf cultivars under salinity stress conditions, its use is recommended.
 

Keywords

• Proline
• Salicylic acid
• Salinity stress
• Tomato
• Vitamin C

Main Subjects

• Growing vegetables

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

References

1. Ahmad, R., Hussain, S., Anjum, M.A., Khalid, M.F., Saqib, M., Zakir, I., & Ahmad, S. (2019). Oxidative stress and antioxidant defense mechanisms in plants under salt stress. Plant abiotic stress tolerance: Agronomic, Molecular and Biotechnological Approaches, 191-205. https://doi.org/10.1007/978-3-030-06118-0_8
2. Ahmadi, Sh. (2017). Effect of sodium nitroprusside and salicylic acid foliar applicationon morpho-physiological characters and postharvest quality of tomatounder salinity stress. University of Kurdistan. Faculty of Agriculture. Department of Horticultural Science.
3. Antonić, D., Milošević, S., Cingel, A., Lojić, M., Trifunović-Momčilov, M., Petrić, M., & Simonović, A. (2016). Effects of exogenous salicylic acid on Impatiens walleriana grown in vitro under polyethylene glycol-imposed drought. South African Journal of Botany, 105, 226-233. https://doi.org/10.1016/j.sajb.2016.04.002
4. )1980(. Official methods of analysis. 13^th Ed. Washington, D. C., Association of Official Analytical Chemists.
5. Bates, L.S., Waldren, R.A., & Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060
6. Bayat, H., Alirezaie, M., & Neamati, H. (2012). Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis) under salinity stress. Journal of Stress Physiology & Biochemistry, 8(1), 258-267.
7. Cheng, X., Deng, G., Su, Y., Liu, J.J., Yang, Y., Du, G.H., & Liu, F.H. (2016). Protein mechanisms in response to NaCl-stress of salt-tolerant and salt-sensitive industrial hemp based on iTRAQ technology. Industrial Crops and Products, 83, 444-452. https://doi.org/10.1016/j.indcrop.2015.12.086
8. Egamberdieva, D., Wirth, S., Bellingrath-Kimura, S.D., Mishra, J., & Arora, N.K. (2019). Salt-tolerant plant growth promoting rhizobacteria for enhancing crop productivity of saline soils. Frontiers in Microbiology, 10, 2791. https://doi.org/10.3389/fmicb.2019.02791
9. Farhadi, N., & Ghassemi-Golezani, K. (2020). Physiological changes of Mentha pulegium in response to exogenous salicylic acid under salinity. Scientia Horticulturae, 267, 109325. https://doi.org/10.1016/j.scienta.2020.109325
10. Flowers, T.J. (2004). Improving crop salt tolerance. Journal of Experimental botany, 55(396), 307-319. https://doi.org/10.1093/jxb/erh003
11. Gharib, F.A. (2006). Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agriculture and Biology, 4, 485-492.
12. Haghighi, M., & Mansouri, F. (2019). Effect of jasmonic acid and salicylic acid on growth and physiological changes of tomato under salinity stress. Journal of Soil and Plant Interactions-Isfahan University of Technology, 9(4), 1-14. http://dorl.net/dor/20.1001.1.20089082.1397.9.4.1.7
13. Hajiaghaei-Kamrani, M., & Hosseinniya, H. (2013). Effect of salinity on nutrient uptake in tomato (Lycopersicon esculentum) in hydroponic system. International Journal of Agronomy and Plant Production, 4(10), 2729-2733.
14. Horváth, E., Csiszár, J., Gallé, Á., Poór, P., Szepesi, Á., & Tari, I. (2015). Hardening with salicylic acid induces concentration-dependent changes in abscisic acid biosynthesis of tomato under salt stress. Journal of Plant Physiology, 183, 54-63. https://doi.org/10.1016/j.jplph.2015.05.010
15. Hussein, M.M., Balbaa, L.K., & Gaballah, M.S. (2007). Salicylic acid and salinity effects on growth of maize plants. Research Journal of Agriculture and Biological Sciences, 3(4), 321-328.
16. Jayakannan, M., Bose, J., Babourina, O., Rengel, Z., & Shabala, S. (2015). Salicylic acid in plant salinity stress signalling and tolerance. Plant Growth Regulation, 76, 25-40. https://doi.org/10.1007/s10725-015-0028-z
17. Kazemi, M. (2013). Influence of foliar application of 5-sulfosalicylic acid, malic acid, putrescine and potassium nitrate on vegetative growth and reproductive characteristics of strawberry cv.‘Selva’. Journal of Biological and Environmental Sciences, 7(20), 93-101.
18. Khorsandi, O., Hassani, A., Sefidkon, F., Shirzad, H., & Khorsandi, A.R. (2010). Effect of salinity (NaCl) on growth, yield, essential oil content and composition of Agastache foeniculum Iranian Journal of Medicinal and Aromatic Plants Research, 26(3), 438-451. https://doi.org/10.22092/ijmapr.2010.6807
19. Khoshbakht, D., & Asgharei, M.R. (2015). Influence of foliar-applied salicylic acid on growth, gas-exchange characteristics, and chlorophyll fluorescence in citrus under saline conditions. Photosynthetica, 53, 410-418.
20. Kiani, A.R., & Mirlatifi, S.M. (2012). Effect of different quantities of supplemental irrigation and its salinity on yield and water use of winter wheat (Triticum aestivum). Irrigation and Drainage, 61(1), 89-98. https://doi.org/10.1002/ird.629
21. Li, T., Hu, Y., Du, X., Tang, H., Shen, C., & Wu, J. (2014). Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis Merrillii seedlings by activating photosynthesis and enhancing antioxidant systems. PLOS one, 9(10), e109492.
22. Ma, X., Zheng, J., Zhang, X., Hu, Q., & Qian, R. (2017). Salicylic acid alleviates the adverse effects of salt stress on Dianthus superbus (Caryophyllaceae) by activating photosynthesis, protecting morphological structure, and enhancing the antioxidant system. Frontiers in Plant Science, 8, 600. https://doi.org/10.3389/fpls.2017.00600
23. Massaretto, I.L., Albaladejo, I., Purgatto, E., Flores, F.B., Plasencia, F., Egea-Fernández, J.M., & Egea, I. (2018). Recovering tomato landraces to simultaneously improve fruit yield and nutritional quality against salt stress. Frontiers in Plant Science, 9, https://doi.org/10.3389/fpls.2018.01778
24. Mohammadi, M., Khosravifar, F., & Siahi, N. (2024). The effect of glycine betaine on some morphological traits, osmolyte accumulations and antioxidant system of sports grass under salt stress. Journal of Horticultural Science. https://doi.org/10.22067/jhs.2024.85599.1305
25. Mohammadzadeh, M., Arouee, H., Neamati, S.H., & Shoor, M. (2013). Effect of different levels of salt stress and salicylic acid on morphological characteristics of four mass native basils (Ocimum basilcum). International Journal of Agronomy and Plant Production, 4(Special Issue), 3590-3596.
26. Nazar, R., Iqbal, N., Syeed, S., & Khan, N.A. (2011). Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars. Journal of Plant Physiology, 168(8), 807-815. https://doi.org/10.1016/j.jplph.2010.11.001
27. Nounjan, N., Nghia, P.T., & Theerakulpisut, P. (2012). Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes. Journal of Plant Physiology, 169(6), 596-604. https://doi.org/10.1016/j.jplph.2012.01.004
28. Pessarakli, M. (2016). Saltgrass, a minimum water and nutrient requirement halophytic plant species for sustainable agriculture in desert regions. Journal of Earth, Environment and Health Sciences, 2(1), 21.
29. Piri, H., Ansari, H., & Parsa, M. (2018). Determination of water- salinity production function by taking time performance and the assessment production indexes of forage sorghum. Water Resour, 11, 38. 15-26.
30. Poór, P., Borbély, P., Bódi, N., Bagyánszki, M., & Tari, I. (2019). Effects of salicylic acid on photosynthetic activity and chloroplast morphology under light and prolonged darkness. Photosynthetica, 57(2).
31. Putra, P.A., & Yuliando, H. (2015). Soilless culture system to support water use efficiency and product quality: a review. Agriculture and Agricultural Science Procedia, 3, 283-288. https://doi.org/10.1016/j.aaspro.2015.01.054
32. Rady, M.M., & Mohamed, G.F. (2015). Modulation of salt stress effects on the growth, physio-chemical attributes and yields of Phaseolus vulgaris plants by the combined application of salicylic acid and Moringa oleifera leaf extract. Scientia Horticulturae, 193, 105-113. https://doi.org/10.1016/j.scienta.2015.07.003
33. Rezvan, A., Eftekhari, S., Salehi, R., & Sedighi Dehkordi, F. (2022). Comparison of yield, fruit quality and some biochemical traits of four cherry tomato varieties in soilless culture. Journal of Horticultural Science, 35(4), 459-468. https://doi.org/10.22067/jhs.2021.60924.0
34. Rivas-San Vicente, M., & Plasencia, J. (2011). Salicylic acid beyond defence: its role in plant growth and development. Journal of Experimental Botany, 62(10), 3321-3338. https://doi.org/10.1093/jxb/err031
35. Mohamadiyeh, Z.S., Moghaddam, M., Abedy, B., & Samiei, L. (2015). Effects of salinity stress on some yield parameters and morphological characteristics of spearmint (Mentha spicata) in hydroponic conditions. Journal of Science and Technology of Greenhouse Culture, 6(23).
36. Salehi, S., Babalar, M., Tagvi, T., & Askari Sarcheshmeh, M.A. (2012). The effect of salicylic acid misting treatment on the growth, yield and quality traits of Camarosa strawberry. Journal of Horticultural Sciences of Iran, 44, 349-357.
37. Signore, A., Serio, F., & Santamaria, P. (2016). A targeted management of the nutrient solution in a soilless tomato crop according to plant needs. Frontiers in Plant Science, 7, 391. https://doi.org/10.3389/fpls.2016.00391
38. Weisany, W., Sohrabi, Y., Heidari, GH., & Ghassemi-Golzani, K. (2011). Physiological responses of soybean (Glycine max) to zinc application under salinity stress. Australian Journal of Crop Science, 5(11), 1441-1447.