Document Type : Research Article
Authors
Ferdowsi university of Mashhad
Abstract
Abstract
Gerbera is one of the most demanded plants in commercial cut flowers plants. Optimizing the micro-propagation of new cultivars is therefore important. In this research “Red Explosion” cultivar was used and Capitola (as explants) were directed to a modified solid Murashigue and Skoog medium with four concentration of TDZ (0.25, 0.50, 0.75 and 1.00 mgL-1) for regeneration. Three concentration of kinetin (4, 6 and 8 mgL-1) were applied and plantlets were transferred to free hormone medium for rooting. Number of new leaves produced, and fresh mass accumulation of cultured plantlets were used to determine the growth rate at 20 and 40 days after culture. Results showed that maximum shoot regeneration was occurred in 0.75 and 1.0 mgL-1TDZ. In proliferation stage first 20 days maximum increase in fresh weight in 6 mgL-1 kinetin and in second 20 days after culture in medium contained 4mgL-1 kinetin were observed. The highest number of new leaves was produced on the medium containing 6 mgL-1 kinetin and 87% of plantlets rooted after one month.
Key words: Gerbera, Tissue culture, Thidiazuron, Kinetin
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